Nanostructured materials characterized by scanning photoelectron spectromicroscopy

• Matteo Amati,
• Alexey S. Shkvarin,
• Alexander I. Merentsov,
• Alexander N. Titov,
• María Taeño,
• David Maestre,
• Sarah R. McKibbin,
• Zygmunt Milosz,
• Ana Cremades,
• Rainer Timm and
• Luca Gregoratti

Beilstein J. Nanotechnol. 2025, 16, 700–710, doi:10.3762/bjnano.16.54

• decades, many characterization tools used to investigate chemical, electronic, or structural properties of materials have been developed or upgraded to match the requirements imposed by nanotechnology. Imaging capabilities covering from meso- to atomic scales, spatially resolved spectroscopies with

• four decades, several important upgrades have spread the original ability of XPS of chemical analysis to include, for instance, band mapping through angle resolved measurements (ARPES), spin detection, and imaging or spectromicroscopy at a nanoscale spatial resolution [9][10]. It is worth noting that

• several improvements have been developed at synchrotron light facilities where unique properties of X-ray radiation can be found. Scanning photoelectron microscopy (SPEM) combines XPS analysis with lateral resolution; chemical imaging as well as XPS spectroscopy at nanoscale sized areas can be performed

Colloidal few layered graphene–tannic acid preserves the biocompatibility of periodontal ligament cells

• Teissir Ben Ammar,
• Naji Kharouf,
• Dominique Vautier,
• Housseinou Ba,
• Nivedita Sudheer,
• Philippe Lavalle and
• Vincent Ball

Beilstein J. Nanotechnol. 2025, 16, 664–677, doi:10.3762/bjnano.16.51

• was used for imaging, using excitation/emission wavelengths of 485/530 nm. DMSO-treated cells served as a control group. All procedures were carried out in darkness to prevent light exposure. The ImageJ software was used to analyze fluorescence intensity in microscopy images. Results are expressed as

Nanoscale capacitance spectroscopy based on multifrequency electrostatic force microscopy

• Pascal N. Rohrbeck,
• Lukas D. Cavar,
• Franjo Weber,
• Peter G. Reichel,
• Mara Niebling and
• Stefan A. L. Weber

Beilstein J. Nanotechnol. 2025, 16, 637–651, doi:10.3762/bjnano.16.49

• its performance with established techniques through capacitance imaging of a model microcapacitor system and a perfluoroalkyl-alkane F(CF2)14(CH2)20H (F14H20) sample. Theory Multifrequency electrostatic force microscopy The electrostatic force FES between tip and sample can be understood in terms of

• of the long-range interactions from tip cone and cantilever in the C′ signal. Thus, the overall impact of the local surface dielectric properties under the tip apex is reduced as compared to the impact of the dielectric properties probed by tip cone and cantilever (see Figure 3). Imaging C′ versus C

• ″ To demonstrate the capabilities of MFH-EFM as an imaging method, we performed experiments on self-assembled nanostructures consisting of the amphiphilic molecule F14H20 (Figure 7). F14H20 exhibits a strong dipole moment of 3.1 D oriented along the chains consisting of fluorinated and hydrogenated

Polyurethane/silk fibroin-based electrospun membranes for wound healing and skin substitute applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Muhammad Waqas,
• Ahsan Nazir and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 591–612, doi:10.3762/bjnano.16.46

• efficiency. In imaging and histological tests, the SF/CS/nHA-BMSC scaffold combined with CGF significantly improved bone repair compared to the control groups [89]. Safdari et al. incorporated ceftazimide (STZ) to develop antibiotic-loaded SF/gelatin nanofibers through electrospinning for wound dressing

Nanomaterials in targeting amyloid-β oligomers: current advances and future directions for Alzheimer's disease diagnosis and therapy

• Shiwani Randhawa,
• Trilok Chand Saini,
• Manik Bathla,
• Rahul Bhardwaj,
• Rubina Dhiman and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2025, 16, 561–580, doi:10.3762/bjnano.16.44

• of AD by targeting AβOs is crucial for improving outcomes. Current imaging methods, such as computed tomography (CT)/positron emission tomography (PET) with FDA-approved 18F-radiotracers (e.g., Amyvid™ and Tauvid™), detect plaques and tau tangles but not AβOs [13]. There is an urgent need for agents

• biosensors for early diagnosis and improving the sensitivity of AβO detection. In imaging, nanoparticles (NPs) can help to visualize localized protein accumulation, complementing existing diagnostic methods. Materials such as carbon-based NMs (e.g., graphene oxide) and metal NPs (e.g., gold and silver

• ) enhance imaging sensitivity because of their distinct electrical or photoluminescent properties. For treatment, NPs can serve as drug carriers, improving delivery across the BBB and reducing side effects. Their large surface area allows for controlled drug release and targeted therapy, enhancing treatment

Functionalized gold nanoflowers on carbon screen-printed electrodes: an electrochemical platform for biosensing hemagglutinin protein of influenza A H1N1 virus

• Carlos Enrique Torres-Méndez,
• Sharmilee Nandi,
• Klara Martinovic,
• Patrizia Kühne,
• Yifan Liu,
• Sam Taylor,
• Maria Lysandrou,
• Maria Ines Berrojo Romeyro Mascarenhas,
• Viktoria Langwallner,
• Javier Enrique Sebastián Alonso,
• Ivana Jovanovic,
• Maike Lüftner,
• Georgia-Vasiliki Gkountana,
• David Bern,
• Abdul-Raouf Atif,
• Ehsan Manouchehri Doulabi,
• Gemma Mestres and
• Masood Kamali-Moghaddam

Beilstein J. Nanotechnol. 2025, 16, 540–550, doi:10.3762/bjnano.16.42

• . DPV measurements were performed with equilibration of 5 s, scanning potential from 0.3 to −0.3 V (vs Ag/AgCl), Estep = 0.01 V, Epulse = 0.086 V, and tpulse = 4 s at a scan rate of 100 mV/s. SEM analysis of AuNFs Prior to SEM imaging, the electrode samples were sputtered with a layer of gold/palladium

Electron beam-based direct writing of nanostructures using a palladium β-ketoesterate complex

• Chinmai Sai Jureddy,
• Krzysztof Maćkosz,
• Aleksandra Butrymowicz-Kubiak,
• Iwona B. Szymańska,
• Patrik Hoffmann and
• Ivo Utke

Beilstein J. Nanotechnol. 2025, 16, 530–539, doi:10.3762/bjnano.16.41

• 0.35 nA. The operating pressure was 1.5 × 10−5 mbar. Results and Discussion The square deposit, fabricated on the native-oxide Si(100) substrate using a spiral inward scanning strategy, was analyzed to assess its morphology and structure. Secondary electron detection in SEM imaging reveals the

• prepared on an ultrathin carbon support film spanning a lacey carbon membrane (Figure 2c). The deposit appears smeared because of drift caused by charging effects during the deposition process. High-resolution STEM imaging (Figure 2d,e) revealed a granular nanostructure with nanograins of around 2 nm in

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

Biomimetics and bioinspired surfaces: from nature to theory and applications

• Rhainer Guillermo Ferreira,
• Thies H. Büscher,
• Manuela Rebora,
• Poramate Manoonpong,
• Zhendong Dai and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2025, 16, 418–421, doi:10.3762/bjnano.16.32

• , substrate compliance, and overall pad geometry. The attachment system of a second stick insect species was structurally investigated by Thomas et al. [4]. This article employed a range of imaging techniques to elucidate the ultrastructure and material composition of the two attachment pad types of this

Size control of nanoparticles synthesized by pulsed laser ablation in liquids using donut-shaped beams

• Abdel Rahman Altakroury,
• Oleksandr Gatsa,
• Farbod Riahi,
• Zongwen Fu,
• Miroslava Flimelová,
• Andrei Samokhvalov,
• Stephan Barcikowski,
• Carlos Doñate-Buendía,
• Alexander V. Bulgakov and
• Bilal Gökce

Beilstein J. Nanotechnol. 2025, 16, 407–417, doi:10.3762/bjnano.16.31

• GROC·UJI, Institute of New Imaging Technologies, Universitat Jaume I, Av. de Vicent Sos Baynat, s/n, 12071 Castellón, Spain 10.3762/bjnano.16.31 Abstract The potential to modify the size distribution of nanoparticles synthesized by pulsed laser ablation in liquids is demonstrated using a donut-shaped

• the donut-shaped laser beam. We performed time-resolved shadowgraph imaging of the laser-induced cavitation bubble, revealing a toroidal structure that overruns the ring-shaped ablation site, compared to the quasi-hemispherical bubble covering the ablation spot produced by the Gaussian beam. Based on

ReactorAFM/STM – dynamic reactions on surfaces at elevated temperature and atmospheric pressure

• Tycho Roorda,
• Hamed Achour,
• Matthijs A. van Spronsen,
• Marta E. Cañas-Ventura,
• Sander B. Roobol,
• Willem Onderwaater,
• Mirthe Bergman,
• Peter van der Tuijn,
• Gertjan van Baarle,
• Johan W. Bakker,
• Joost W. M. Frenken and
• Irene M. N. Groot

Beilstein J. Nanotechnol. 2025, 16, 397–406, doi:10.3762/bjnano.16.30

• structure of materials under reaction conditions. We demonstrate this by imaging a Pd(100) single crystal at 450 K with combined AFM/STM. The surface is compared under ultrahigh vacuum and under 0.5 bar O2 pressure showing a notable increase in RMS current, which we attribute to oxidation. Also, we study

Engineered PEG–PCL nanoparticles enable sensitive and selective detection of sodium dodecyl sulfate: a qualitative and quantitative analysis

• Soni Prajapati and
• Ranjana Singh

Beilstein J. Nanotechnol. 2025, 16, 385–396, doi:10.3762/bjnano.16.29

• SDS concentration of 0.1% to 0.00625% for 30 min showed its toxicity in terms of loss of focus, lens transparency, significant increase in lens wet weight, and axial length. Confocal imaging confirmed concentration-dependent mitochondrial loss in the ocular lens [8]. Human exposure to SDS via air was

• delivery, targeting, sensing, and imaging [23]. Also, there are a wide variety of nanoparticles available for desired applications. In the case of detection of contaminants or sensing applications, carbon and metal nanoparticles are mostly preferable [24]. Despite their use, these nanoparticles possess

• nanoparticles is a step ahead of the reported studies. Polymer nanoparticles prepared from PEG–PCL are widely used for drug delivery, tumour targeting, and imaging [28]. To our knowledge, there is no report regarding using PEG–PCL nanoparticles (PEG–PCL NPs) as a contaminant detection system. Accordingly, we

Pulsed laser in liquid grafting of gold nanoparticle–carbon support composites

• Madeleine K. Wilsey,
• Teona Taseska,
• Qishen Lyu,
• Connor P. Cox and
• Astrid M. Müller

Beilstein J. Nanotechnol. 2025, 16, 349–361, doi:10.3762/bjnano.16.26

• , rapid, scalable, acid-free process to make carbon fiber paper hydrophilic without destroying the carbon network, as other carbon fiber paper oxidation methods do [22], evident from scanning electron microscopy (SEM) imaging (Figure 2A). Hydrophilicity was achieved by graphitic edge carbon oxygenation

Graphene oxide–chloroquine conjugate induces DNA damage in A549 lung cancer cells through autophagy modulation

• Braham Dutt Arya,
• Sandeep Mittal,
• Prachi Joshi,
• Alok Kumar Pandey,
• Jaime E. Ramirez-Vick,
• Govind Gupta and
• Surinder P. Singh

Beilstein J. Nanotechnol. 2025, 16, 316–332, doi:10.3762/bjnano.16.24

• and an image analysis system (Andor Technology, Belfast, UK) and a software (KOMET 5.0, Kinetic Imaging, UK) at 400× magnification. Three independent experiments were performed for each group. The mean value of three Comet parameters, tail DNA (%), tail length (μm), and Olive tail moment (OTM) were

• fluorescent microscope equipped with a Nikon Digital slight Ds-Ri1 CCD camera and a NIS element BR imaging software (Nikon, Minato Tokyo, Japan). Three independent experiments were performed for each group and a representative image is shown in the results. Transfection of the GFP-LC3 plasmid The GFP-LC3

Fabrication and evaluation of BerNPs regarding the growth and development of Streptococcus mutans

• Tuyen Huu Nguyen,
• Hong Thanh Pham,
• Kieu Kim Thanh Nguyen,
• Loan Hong Ngo,
• Anh Ngoc Tuan Mai,
• Thu Hoang Anh Lam,
• Ngan Thi Kim Phan,
• Dung Tien Pham,
• Duong Thuy Hoang,
• Thuc Dong Nguyen and
• Lien Thi Xuan Truong

Beilstein J. Nanotechnol. 2025, 16, 308–315, doi:10.3762/bjnano.16.23

• tubes were incubated at 37 °C. After 24 h, the samples were fixed onto a graphite substrate, and FE-SEM imaging was performed. Inhibition of biofilm formation The Crystal Violet Biofilm Assay was used to evaluate the biofilm formation of S. mutans [43]. BerNPs solution was diluted in TSB + 1% sucrose

Enhancing mechanical properties of chitosan/PVA electrospun nanofibers: a comprehensive review

• Nur Areisman Mohd Salleh,
• Amalina Muhammad Afifi,
• Fathiah Mohamed Zuki and
• Hanna Sofia SalehHudin

Beilstein J. Nanotechnol. 2025, 16, 286–307, doi:10.3762/bjnano.16.22

• not impossible. This was demonstrated by Olvera Bernal et al. [58] who employed differentially interferential contrasting to accentuate the color contrast for the fiber diameter measurements of electrospun chitosan/PVA. Imaging techniques based on the principles of electron beams such as scanning

• microscopy has been used to investigate morphology as well as the nanomechanical properties of individual fibers, including magnetization, friction, and mechanical strength [147]. These imaging techniques are critical for linking the mechanical behavior to the microstructure of the fibers. Based on fiber

Radiosensitizing properties of dual-functionalized carbon nanostructures loaded with temozolomide

• Radmila Milenkovska,
• Nikola Geskovski,
• Dushko Shalabalija,
• Ljubica Mihailova,
• Petre Makreski,
• Dushko Lukarski,
• Igor Stojkovski,
• Maja Simonoska Crcarevska and
• Kristina Mladenovska

Beilstein J. Nanotechnol. 2025, 16, 229–251, doi:10.3762/bjnano.16.18

• structures attached to the graphene sheets, attributed dominantly to PEG6000. No clear distinction regarding the morphology of single- and dual-functionalized CNs and structures with and without TMZ could be made with the used imaging technique. Also, no morphological differences were observed between the

Recent advances in photothermal nanomaterials for ophthalmic applications

• Jiayuan Zhuang,
• Linhui Jia,
• Chenghao Li,
• Rui Yang,
• Jiapeng Wang,
• Wen-an Wang,
• Heng Zhou and
• Xiangxia Luo

Beilstein J. Nanotechnol. 2025, 16, 195–215, doi:10.3762/bjnano.16.16

• boundaries of thermal therapy, achieving unparalleled therapeutic effects through their diverse composite structures and demonstrating enormous potential in promoting retinal drug delivery and photoacoustic imaging. This paper provides a comprehensive summary of the structure–activity relationship between

• (Figure 1). In addition, the application progress of photothermal nanomaterials in promoting retinal drug delivery and enhancing photoacoustic imaging was also discussed. Finally, we address the current challenges and prospects in ophthalmic treatment technologies based on photothermal nanomaterials, with

• synthesis of AuNPs and served as a surface coating for AuNPs, promoting the coupling of DOX, enhancing anti-tumor activity, and improving the biocompatibility of AuNPs. The significant extinction coefficient of these nanoparticles enhances the contrast in photoacoustic imaging within the tumor region

Probing the potential of rare earth elements in the development of new anticancer drugs: single molecule studies

• Josiane A. D. Batista,
• Rayane M. de Oliveira,
• Carlos H. M. Lima,
• Milton L. Lana Júnior,
• Virgílio C. dos Anjos,
• Maria J. V. Bell and
• Márcio S. Rocha

Beilstein J. Nanotechnol. 2025, 16, 187–194, doi:10.3762/bjnano.16.15

• extracted as well, providing robust information about the effects of the rare earths on the DNA double helix [19][16]. In addition, atomic force microscopy (AFM) imaging assays were also performed to confirm DNA compaction/condensation by erbium and neodymium, allowing for a direct visualization of these

A review of metal-organic frameworks and polymers in mixed matrix membranes for CO₂ capture

• Charlotte Skjold Qvist Christensen,
• Nicholas Hansen,
• Mahboubeh Motadayen,
• Nina Lock,
• Martin Lahn Henriksen and
• Jonathan Quinson

Beilstein J. Nanotechnol. 2025, 16, 155–186, doi:10.3762/bjnano.16.14

• MMMs Material characterization techniques are pivotal for the assessment of novel MOF-based MMMs structures. Electron microscopy enables direct imaging of a sample with up to sub-nanometer resolution [139]. Scanning electron microscopy (SEM) is a popular and straightforward method to obtain images of

Instance maps as an organising concept for complex experimental workflows as demonstrated for (nano)material safety research

• Benjamin Punz,
• Maja Brajnik,
• Joh Dokler,
• Jaleesia D. Amos,
• Litty Johnson,
• Katie Reilly,
• Anastasios G. Papadiamantis,
• Amaia Green Etxabe,
• Lee Walker,
• Diego S. T. Martinez,
• Steffi Friedrichs,
• Klaus M. Weltring,
• Nazende Günday-Türeli,
• Claus Svendsen,
• Christine Ogilvie Hendren,
• Mark R. Wiesner,
• Martin Himly,
• Iseult Lynch and
• Thomas E. Exner

Beilstein J. Nanotechnol. 2025, 16, 57–77, doi:10.3762/bjnano.16.7

• for a proof-of-concept of the treatment of antibiotic-resistant bacterial lung infections. In addition, controls are prepared for imaging purposes to verify the suitability of the MACRAMÉ approach to quantify and characterise the aerosols upon exposure of in vitro lung models. Relevant exposure points

A nanocarrier containing carboxylic and histamine groups with dual action: acetylcholine hydrolysis and antidote atropine delivery

• Elina E. Mansurova,
• Andrey A. Maslennikov,
• Anna P. Lyubina,
• Alexandra D. Voloshina,
• Irek R. Nizameev,
• Marsil K. Kadirov,
• Anzhela A. Mikhailova,
• Polina V. Mikshina,
• Albina Y. Ziganshina and
• Igor S. Antipin

Beilstein J. Nanotechnol. 2025, 16, 11–24, doi:10.3762/bjnano.16.2

• Cytell Cell Imaging multifunctional system (GE Healthcare Life Science, Sweden) [38]. The experiments utilized a Chang liver cell line (human liver cells) from the N.F. Gamaleya National Research Center for Epidemiology and Microbiology and a cell culture of WI-38 VA 13 subline 2RA (human embryo lung

Orientation-dependent photonic bandgaps in gold-dust weevil scales and their titania bioreplicates

• Norma Salvadores Farran,
• Limin Wang,
• Primoz Pirih and
• Bodo D. Wilts

Beilstein J. Nanotechnol. 2025, 16, 1–10, doi:10.3762/bjnano.16.1

• that their orientation and nature can be identified using reciprocal k-space imaging [28]. To see whether the gold-dust weevil scales contain diamond photonic crystal networks, we performed reciprocal space imaging using a 50× (NA = 0.95) objective. The real space images with full-aperture illumination

• are presented in Supporting Information File 1, Figure S6. Reciprocal space imaging also allows one to map the bandgap diagram. For this, we measured reflectance spectra in the reciprocal space using full-aperture illumination from a domain that was almost perfectly {100}-oriented (Figure 3d). The

• series [5×, 10×, 20×, 50×, and 100×] with apertures [NA = 0.13, 0.30, 0.60, 0.95, and 0.95], respectively. For reciprocal space imaging, we used the 50× and 100× objectives. The 100× objective has a smaller minimal illumination spot (field), but a bigger minimal aperture (angle of the illumination cone

Liver-targeting iron oxide nanoparticles and their complexes with plant extracts for biocompatibility

• Shushanik A. Kazaryan,
• Seda A. Oganian,
• Gayane S. Vardanyan,
• Anatolie S. Sidorenko and
• Ashkhen A. Hovhannisyan

Beilstein J. Nanotechnol. 2024, 15, 1593–1602, doi:10.3762/bjnano.15.125

• Fe3O4 NPs have great potential for commercial use and have already found applications in biomedicine, such as magnetic resonance imaging (as contrast enhancement agents), targeted drug or gene delivery, tissue engineering, biological fluid detoxification, hyperthermia, biological sensing, nanozymes, and

Facile synthesis of size-tunable L-carnosine-capped silver nanoparticles and their role in metal ion sensing and catalytic degradation of p-nitrophenol

• Akash Kumar,
• Ridhima Chadha,
• Abhishek Das,
• Nandita Maiti and
• Rayavarapu Raja Gopal

Beilstein J. Nanotechnol. 2024, 15, 1576–1592, doi:10.3762/bjnano.15.124