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Search for "room temperature" in Full Text gives 1416 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.
New application of bimetallic Ag/Pt nanoplates in a colorimetric biosensor for specific detection of E. coli in water
Beilstein J. Nanotechnol. 2024, 15, 95–103, doi:10.3762/bjnano.15.9
- room temperature using a Zetasizer Nano-ZS90 Malvern. The elementary compositions of the samples were determined using EDS on a Tescan energy-dispersive spectrometer. The ImageJ software was used to determine the gray intensity of the blue color of the paper-based microfluidic device. Synthesis of Ag
- (10–108 CFU·mL−1) were combined with the aptamer-Ag/Pt NPL solution and gently shaken at room temperature. The peroxidase activity of NPLs in the presence of TMB and H2O2 was evaluated using a UV–vis spectrophotometer after the incubation period was shortened. Analysis in real samples Tap water and
Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface
Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8
- NF sample) and incubated at room temperature for 10 min two times. The stripping buffer was removed from the nanofibers, and the NFs were washed with PBS (adding and incubating for 10 min two times). The two stripping buffer steps were repeated and three 5 min PBS wash steps took place after them
- /mL) against ricin was incubated for 1 h at room temperature. Then, biotin-labelled ricin (1 µL 1 mg/mL in blocking buffer) was immunocaptured by the anchored antibodies over 1 h of incubation. Biotin-ricin was added to both NF and microplate immunocapture systems at different times: day 0
- (immediately after antibody anchoring; it is considered the reference value) and 7 days, 30 days, and 90 days after antibody anchoring, bringing the immunocapture systems to room temperature only covered by aluminum foil. Washing steps were carried out between each step above in order to remove non-linked
Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency
Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7
- /PLA, and BBR NPs/PLA were fabricated through the electrospinning of the aforementioned prepared solutions. After the solutions were cooled down to room temperature, they were transferred to a 5 mL syringe with a 22-gauge stainless-steel needle. The needle was linked to a high-voltage power supply
- Multi-function shaker at room temperature with constant agitation at 40 rpm. At each time interval, 2 mL of each solution was withdrawn, the UV–vis absorbance at 421 nm was measured, and then the amount of BBR release based on the standard calibration curve was calculated. The percentage of released BBR
Curcumin-loaded nanostructured systems for treatment of leishmaniasis: a review
Beilstein J. Nanotechnol. 2024, 15, 37–50, doi:10.3762/bjnano.15.4
- following the Ambisome® path. Nanostructured lipid carriers Nanostructured lipid carriers (NLCs) are lipid-based formulations with a solid matrix at room temperature that differ from solid lipid nanoparticles when it comes to their matrix organizational level. Nanostructured lipid carriers offer advantages
Measurements of dichroic bow-tie antenna arrays with integrated cold-electron bolometers using YBCO oscillators
Beilstein J. Nanotechnol. 2024, 15, 26–36, doi:10.3762/bjnano.15.3
- measured in the current bias mode with a readout scheme based on AD745 low-noise JFET operational amplifiers working at room temperature of 300 K. The estimated NEP for this measurement setup is about 5 × 10−16 W/√Hz with further improvement down to 1 × 10−16 W/√Hz in the voltage bias mode [24]. Discussion
TEM sample preparation of lithographically patterned permalloy nanostructures on silicon nitride membranes
Beilstein J. Nanotechnol. 2024, 15, 1–12, doi:10.3762/bjnano.15.1
- magnetron sputtering through the resist aperture. We used DC magnetron sputtering in a pure Ar environment at a pressure of 1 Pa to deposit Py at room temperature. The effective permalloy target had a diameter of 8 mm. The sputtered material almost forms a parallel beam when it approaches the substrate at a
unDrift: A versatile software for fast offline SPM image drift correction
Beilstein J. Nanotechnol. 2023, 14, 1225–1237, doi:10.3762/bjnano.14.101
- ]. Hence, many processes relevant at room temperature or elevated temperatures are impossible to study in a cryogenic environment. The same applies to SPM studies at the solid–liquid interface. For these measurements, the effect of thermal drift needs to be compensated. A variety of different strategies
Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells
Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100
- . Compared to the quantum yields of FITC, RhBITC, and DAPI bound to DNA, which are in the range of 40–90% at room temperature [31][32][34], Ox-BSA/PDA NPs reveal low emission efficiency. However, even if the quantum yield of Ox-BSA/PDA NPs is low, it is significantly higher than the quantum yield of non
- am Harz, Germany) for 48 h to provide lyophilized foam, which was stored at room temperature and protected from light before use. Fluorescent BSA/polydopamine nanoparticles Fluorescent BSA/PDA nanoparticles with a BSA/DA ratio of 10 were synthesized by using two different ways: (i) by oxidation of
- 2 mL of H2O2 solution (30 wt %) were stirred at room temperature in the dark for 24 h. Residual H2O2 was removed from the reaction medium by dialyzing about 8 mL of the suspension against 1 L Milli-Q® water using a Spectra/Por® dialysis membrane with a molecular weight cut-off at 8–10 kDa. The
Determination of the radii of coated and uncoated silicon AFM sharp tips using a height calibration standard grating and a nonlinear regression function
Beilstein J. Nanotechnol. 2023, 14, 1200–1207, doi:10.3762/bjnano.14.99
- calibration grating Linewidth scanning in contact mode using each of the selected tips with an applied constant normal load of 0.01 nN perpendicular to the calibration grating was carried out at room temperature and 40% humidity. The scanning frequency of the tips was 0.03 Hz. The tips were moved from right
A combined gas-phase dissociative ionization, dissociative electron attachment and deposition study on the potential FEBID precursor [Au(CH3)2Cl]2
Beilstein J. Nanotechnol. 2023, 14, 1178–1199, doi:10.3762/bjnano.14.98
Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment
Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96
- , 150 °C for 15 min, 170 °C for 1 h, and 195 °C for 1 h. Afterwards, the sample was allowed to cool down to room temperature (RT), and then the PDMS was peeled off (Figure 1A(iv)). To form the “reinforced” SU-8 nanohole mold, a nanohole template was first formed on the positive photoresist AZ1518, which
Elasticity, an often-overseen parameter in the development of nanoscale drug delivery systems
Beilstein J. Nanotechnol. 2023, 14, 1149–1156, doi:10.3762/bjnano.14.95
- elasticity of nanomaterials. Even though the exact temperature of the particles is not known, it is assumed to be equal to the surrounding temperature after a sufficient equilibration time. Many studies are performed at room temperature or at 25 °C instead of at the temperature in which biologic studies are
Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study
Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93
- solution produced by reverse engineering, and make quality control more challenging [19]. Additionally, the degummed silk tends to aggregate when stored for long periods in an aqueous solution, typically for weeks at room temperature and for months at 4 °C [20]. Amongst various carriers, albumin seems to
- mixed with 20 mL of 0.25 M Na2CO3 under constant vigorous stirring for 30 s at room temperature (RT). HSA solution (0.1%) was subsequently added to the mixture under stirring for 5 min to avoid particle aggregation. The obtained particle suspension was separated by centrifugation (6,000g for 3 min
Dual-heterodyne Kelvin probe force microscopy
Beilstein J. Nanotechnol. 2023, 14, 1068–1084, doi:10.3762/bjnano.14.88
- ) illumination. An SPV image can eventually be recalculated as the difference between both sets of data [1]. The inherent drawback of this dual-pass method is that the source images used for the SPV calculation may feature misalignments, which is especially unavoidable at room temperature (RT) due to thermal
- kHz (demodulation at the second eigenmode) for its heterodyne counterpart. Dual-Heterodyne Kelvin Probe Force Microscopy: Implementation Non-contact AFM (nc-AFM) and KPFM experiments are performed with a VT-beam AFM system (Scienta-Omicron) at room temperature (RT) under ultra-high vacuum (UHV
Experimental investigation of usage of POE lubricants with Al2O3, graphene or CNT nanoparticles in a refrigeration compressor
Beilstein J. Nanotechnol. 2023, 14, 1041–1058, doi:10.3762/bjnano.14.86
- XRD and compared with previous studies [23][24] in the literature. The XRD patterns of the Al2O3 nanoparticle samples were recorded in the range 2θ (15°–75°) at room temperature. The XRD pattern of γ-Al2O3 is shown in Figure 3. The diffraction pattern of γ-Al2O3 shows strong peaks at angle (2θ) of
Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one
Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85
- h at room temperature under stirring. After another washing in aqua dest. the MnCO3 matrix was dissolved using 0.25 M EDTA. After 30 min incubation time, 0.2 mg/mL NaBH4 in 0.1 M NaOH was added to prevent oxidation of Hb to met-Hb. Incubation for another 30 min followed. After triplicate washing in
- NaOH) was added to the particle sample (final concentration: 12.5 mg/mL), mixed, and incubated at room temperature for 15 min while stirring. After centrifugation (20,000g; 10 min) the supernatant was taken off and, immediately, the absorbance at a wavelength of 574 nm was determined photometrically in
- , and CD204, or all of them, were blocked by specific antibodies (CD14-block+HbMP-prefed-30′, CD33-block+HbMP-prefed-30′, CD163-block+HbMP-prefed-30′, CD204-block+HbMP-prefed-30′, and CD14-33-163-204-block+HbMP-prefed-30′). After an incubation period of 30 min protected from light and at room
A visible-light photodetector based on heterojunctions between CuO nanoparticles and ZnO nanorods
Beilstein J. Nanotechnol. 2023, 14, 1018–1027, doi:10.3762/bjnano.14.84
- because of its large exciton binding energy of 60 meV at room temperature [20], excellent chemical and thermal stability, high electron mobility, non-toxicity, low cost, and simple synthesis [21][22]. Various shapes of ZnO nanomaterials can be easily obtained by controlling synthesis conditions (e.g
- sensitivity to other wavelengths including 464 nm (blue), 532 nm (green), and 640 nm (red). Figure 5a shows the room temperature I–V characteristics of the CuO NPs/ZnO NRs photodetector under different wavelengths. The photoresponse to wavelengths longer than 395 nm is in accordance with the UV–vis absorbance
- ) solution in an oven at 95 °C within 3 h (as presented in Supporting Information File 1, Figure S1). CuO NPs were synthesized via a simple and low-cost sol–gel method. At first, a mixture of 1.21 g Cu(NO3)2·5H2O and 0.8 g NaOH was dissolved in 50 mL deionized water under constant stirring at room
Upscaling the urea method synthesis of CoAl layered double hydroxides
Beilstein J. Nanotechnol. 2023, 14, 927–938, doi:10.3762/bjnano.14.76
- ; and (3) X25M: [CoCl2] = 250 mM, [AlCl3] = 125 mM, and [urea] = 875 mM. All obtained solids were filtered, washed three times with H2O, H2O/EtOH, and finally with EtOH. The samples were dried at room temperature and kept in desiccators until further characterization. Synthesis of α-Co layered hydroxide
- The α-Co layered hydroxide synthesis was carried out at room temperature by using the epoxide route for a period of 48 h under constant stirring with solutions of [CoCl2] = 10 mM and [NaCl] = 100 mM, in the presence of glycidol, [Gly] = 500 mM. Chemical and structural characterization Powder X-ray
- -ray absorption spectroscopy (XAS) measurements were performed at the BL-22 (CLÆSS) beamline of the ALBA synchrotron (Barcelona, Spain), proposal: 2022097096. XANES and EXAFS Co K edge spectra were measured at room temperature in transmission mode. Absorbents of as-synthesized fresh samples were
Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes
Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73
- behavior at room temperature, and are taken up by macrophages without being toxic for these mammalian cells [9]. The application of SPIONs in treating leishmaniasis has been studied by different groups over the past few years, showing promising and satisfactory results [10][11][12][13]; thus, using SPIONs
N-Heterocyclic carbene-based gold etchants
Beilstein J. Nanotechnol. 2023, 14, 865–871, doi:10.3762/bjnano.14.71
- test solution in 20 mL glass vials. Vials were capped and left at room temperature in a fume hood for the allotted time of the experiment. The tokens were then removed from the test solution and rinsed in the respective organic solvent of the test solution before drying under a stream of 99.998
Ultralow-energy amorphization of contaminated silicon samples investigated by molecular dynamics
Beilstein J. Nanotechnol. 2023, 14, 834–849, doi:10.3762/bjnano.14.68
- cooled down until it reaches room temperature again. All simulations are carried out at 300 K to reproduce Ar beam processes at room temperature. Results and Discussion In Figure 1 the status of the sample after 500 impacts (corresponding to a fluence of 2.6 × 1015 atoms/cm2) is represented for each
Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies
Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66
- % amplitude using an ultrasonic processor (130 Watts, Cole-Parmer, USA) equipped with a 6 mm titanium tip. After the sonication process, NLC-BNZ were obtained by leaving the hot suspension to cool to room temperature. The remaining volume was then measured. Measurement of the encapsulation efficiency
- Instruments, New Castle, DE, USA). Freeze dried formulations of approximately 10 mg were accurately weighed in a platinum pan. Measurements were performed from room temperature to 600 °C at a heating rate of 10 °C/min under nitrogen atmosphere to avoid thermo-oxidative degradation. Attenuated total reflection
Silver-based SERS substrates fabricated using a 3D printed microfluidic device
Beilstein J. Nanotechnol. 2023, 14, 793–803, doi:10.3762/bjnano.14.65
- centrifuged at 12,000 rpm for 5 min to separate the Ag NPs solution and remove the oil phase. Finally, the Ag NPs suspension was stored in a dark vial at room temperature for further experiments. Fabrication of porous silicon (PS) substrate A p-type Si(100) wafer with a resistivity of 0.01–0.09 Ω·cm was used
- submerged in concentrated HNO3, rinsed with deionized water, and then dried at room temperature to produce the PS substrates. Fabrication of the SERS substrate The deposition of Ag particles on the substrates is based on the SAM method. Colloidal Ag NPs solutions were generated at the methanol/air interface
- with the analyte solution (30 µL) and air-dried at room temperature. SERS signals were then collected from random locations. Results and Discussion Fabrication of droplet-based microfluidic device using 3D printing SLA 3D printing is an additive manufacturing technology that employs a laser to
Carboxylic acids and light interact to affect nanoceria stability and dissolution in acidic aqueous environments
Beilstein J. Nanotechnol. 2023, 14, 762–780, doi:10.3762/bjnano.14.63
- transmission electron microscopy (TEM) imaging, samples were sonicated for 10 min in a sonication bath. Lacey carbon, 300 mesh, copper grids were dipped into the solution for approximately 5 s and dried overnight at room temperature. TEM was used to obtain images of particles throughout the experiment’s
In situ magnesiothermic reduction synthesis of a Ge@C composite for high-performance lithium-ion batterie anodes
Beilstein J. Nanotechnol. 2023, 14, 751–761, doi:10.3762/bjnano.14.62
- than Si at room temperature) conductivity [14]. However, electrodes based on alloying suffer from deterioration during repeated lithiation/delithiation because of the large variation in volume. Despite the lower volume change ratio compared to Si (370% for Ge and 400% for Si) and the structural
- at 180 °C for 12 h. After cooling to room temperature, the solid was collected and rinsed with DI water to neutral pH. The dried solid was denoted as Ge/C-HT180. Solid-state reaction coupling to synthesize Ge/C-SS750 composite Relevant amounts of Ge and BC-800 at a mass ratio of 2:5 were well ground
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