Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

• Roberta D'Agata,
• Pasquale Palladino and
• Giuseppe Spoto

Beilstein J. Nanotechnol. 2017, 8, 1–11, doi:10.3762/bjnano.8.1

• AuNPs are increasingly used for biosensing purposes. We report here a study of AuNPs surface-functionalized with streptavidin-biotinylated oligonucleotide, focussing on the role played by the oligonucleotide probes in the stabilization/destabilization of the functionalized nanoparticle dispersion. The

• further studies on oligodeoxyribonucleotide (ODN)-functionalized SA-conjugated AuNPs. SA-conjugated AuNPs have not been so widely studied as thiol-conjugated AuNPs. Nevertheless, ODN-functionalized SA-conjugated AuNPs are largely used for different purposes including biosensing [15][16][17]. Results from

• spectroscopic, dynamic light scattering (DLS), zeta-potential (ζ), transmission electron microscopy (TEM) and SPR investigations of ODN-functionalized SA-mediated AuNPs are here presented with the aim to provide fundamental information useful to improve performances of biosensing assays using SA-mediated AuNPs

From iron coordination compounds to metal oxide nanoparticles

• Mihail Iacob,
• Carmen Racles,
• Codrin Tugui,
• George Stiubianu,
• Adrian Bele,
• Liviu Sacarescu,
• Daniel Timpu and
• Maria Cazacu

Beilstein J. Nanotechnol. 2016, 7, 2074–2087, doi:10.3762/bjnano.7.198

• surfaces are often used for biomedical applications (e.g., biosensing, hyperthermia and MRI) [10]. In biomedical applications, the morphology of the nanoparticle significantly influences both pharmacokinetics and cell uptake [11]. Nanoparticles are also preferred as fillers for polymers to induce certain

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

• Maryam Daneshpour,
• Kobra Omidfar and
• Hossein Ghanbarian

Beilstein J. Nanotechnol. 2016, 7, 2023–2036, doi:10.3762/bjnano.7.193

• pretreatment or amplification. Consequently, our biosensing strategy offers such a promising application to be used for clinical early detection of GC and additionally the screen of any miRNA sequence. Keywords: electrochemical nanobiosensor; gastric cancer; gold–magnetic nanoparticle; miR-106a; Introduction

• -characterized nanomaterial and the complementarity principle of nucleic acid molecules, this electrochemical nanobiosensor showed remarkable accuracy in evaluating miRNA target concentrations via differential pulse voltammetry (DPV) analyses. The principal steps of the biosensing procedure are schematically

• revealed that better streptavidin coating at the primer step can significantly result in higher probe immobilization efficacy and surely elevate the sensitivity and stability of following biosensing procedure. Characterization of modified electrodes and assessment of the fabrication procedure The

Layered composites of PEDOT/PSS/nanoparticles and PEDOT/PSS/phthalocyanines as electron mediators for sensors and biosensors

• Celia García-Hernández,
• Cristina García-Cabezón,
• Fernando Martín-Pedrosa,
• José Antonio De Saja and
• María Luz Rodríguez-Méndez

Beilstein J. Nanotechnol. 2016, 7, 1948–1959, doi:10.3762/bjnano.7.186

• electron mediators to facilitate the transfer of electrons from the enzyme to the electrode [20]. PEDOT/PSS is becoming popular as an electron mediator in biosensing [21][22]. Gold nanoparticles and phthalocyanines have also been positively demonstrated as electron mediators in tyrosinase biosensors [16

• composites of PEDOT/PSS and AuNPs, CuPc or LuPc2 as electron mediators and to investigate the existence of synergistic effects in the electron transfer occurring during biosensing. In order to pursue the two objectives proposed, PEDOT/PSS/EM electrodes were prepared by depositing a layer of AuNPs, CoPc or

Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring

• Niina Halonen,
• Joni Kilpijärvi,
• Maciej Sobocinski,
• Timir Datta-Chaudhuri,
• Antti Hassinen,
• Someshekar B. Prakash,
• Peter Möller,
• Pamela Abshire,
• Sakari Kellokumpu and
• Anita Lloyd Spetz

Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179

• viability screening are lethal to the cells. Hence there is a growing interest in noninvasive, label-free, real-time, data-rich biosensing systems that measure electrical, optical, magnetic, or mass related properties of the biological sample. Such sensing techniques include surface plasmon resonance

• and spread out over the surface of the chip and LTCC. The Dupont 951 LTCC material has also been reported as biocompatible by others [25]. Cell measurements To demonstrate the feasibility of using the LTCC package in biosensing applications, data were recorded for several hours from a CMOS sensor chip

Large-scale fabrication of achiral plasmonic metamaterials with giant chiroptical response

• Morten Slyngborg,
• Yao-Chung Tsao and
• Peter Fojan

Beilstein J. Nanotechnol. 2016, 7, 914–925, doi:10.3762/bjnano.7.83

• previously used for biosensor applications [10]. Furthermore, compared to PCMs fabricated by another scalable technique [29], the CD linewidths of the honeycomb ECMs are more narrow. Hence the heterogeneity and 3D structure has not limited the use of the honeycomb ECMs for biosensing applications. While the

Direct formation of gold nanorods on surfaces using polymer-immobilised gold seeds

• Majid K. Abyaneh,
• Pietro Parisse and
• Loredana Casalis

Beilstein J. Nanotechnol. 2016, 7, 809–816, doi:10.3762/bjnano.7.72

• ], photocatalysis [25], chemical sensing, biosensing [26][27] and surface-enhanced Raman spectroscopy (SERS) [28]. Last but not least, the synthesis process can be easily extended to screen printing or other thick film deposition processes for batch synthesis procedures [29]. Results and Discussion There are

Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

• M. Fátima Barroso,
• M. Alejandra Luna,
• Juan S. Flores Tabares,
• Cristina Delerue-Matos,
• N. Mariano Correa,
• Fernando Moyano and
• Patricia G. Molina

Beilstein J. Nanotechnol. 2016, 7, 655–663, doi:10.3762/bjnano.7.58

Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies

• Claudia Koch,
• Fabian J. Eber,
• Carlos Azucena,
• Alexander Förste,
• Stefan Walheim,
• Thomas Schimmel,
• Alexander M. Bittner,
• Holger Jeske,
• Hartmut Gliemann,
• Sabine Eiben,
• Fania C. Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2016, 7, 613–629, doi:10.3762/bjnano.7.54

• diverse achievements obtained with TMV-based particles, compares them to the progress with related viruses, and focuses on latest results revealing special advantages for enzyme-based biosensing formats, which might be of high interest for diagnostics employing 'systems-on-a-chip'. Keywords: biotemplate

• scaffolding biotemplates in various applications, ranging from biosensing to tumor therapeutics. Hence, in the recent past, the number of reports on the successful construction of advantageous virus-derived hybrid materials and devices has been growing rapidly and reviewed extensively [5][7][8][65][66][67][68

• : the two-step method achieved maximum surface occupation with full enzymatic activity retained on the viral backbones. TMV adapter scaffolds conducive to biosensing layouts A main goal of the case study on TMV enzyme carriers was to find out if biodetection setups would profit from an integration of

Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers

• Ilaria Solano,
• Pietro Parisse,
• Ornella Cavalleri,
• Federico Gramazio,
• Loredana Casalis and
• Maurizio Canepa

Beilstein J. Nanotechnol. 2016, 7, 544–553, doi:10.3762/bjnano.7.48

• ellipsometry; Introduction The design of biosensing devices requires precise immobilization, with controlled orientation, of functional proteins on a surface, generally consisting of a substrate functionalized with a molecular receptor layer [1][2]. After seminal works [3], the interaction of chelated Ni ions

Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

• Maryse D. Nkoua Ngavouka,
• Pietro Capaldo,
• Elena Ambrosetti,
• Giacinto Scoles,
• Loredana Casalis and
• Pietro Parisse

Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20

• -mechanical and electrochemical) and current label-free surface-based biosensing strategies, according to recent literature. As we can see from the table, SPR strategies seem to be the most promising in terms of limit of detection. However, in these devices the surface area is larger, limiting multiplexing

Controlled graphene oxide assembly on silver nanocube monolayers for SERS detection: dependence on nanocube packing procedure

• Martina Banchelli,
• Bruno Tiribilli,
• Roberto Pini,
• Luigi Dei,
• Paolo Matteini and
• Gabriella Caminati

Beilstein J. Nanotechnol. 2016, 7, 9–21, doi:10.3762/bjnano.7.2

• ], optoelectronics [3], energy-harvesting applications [4], cancer imaging and therapy [5], sensing and biosensing applications [6][7]. In particular, sensors based on arrays of noble metal nanoparticles have become increasingly popular for the ultrasensitive detection of a variety of species ranging from small

Self-organization of gold nanoparticles on silanated surfaces

• Htet H. Kyaw,
• Salim H. Al-Harthi,
• Azzouz Sellai and
• Joydeep Dutta

Beilstein J. Nanotechnol. 2015, 6, 2345–2353, doi:10.3762/bjnano.6.242

• structures [8]. AuNPs have been studied intensively for a wide range of applications such as catalysis [9], biosensing [10], colorimetric sensing [11], optical sensing (surface plasmon resonance (SPR) and surface-enhanced Raman scattering (SERS)) [12][13], photonics [13][14], photovoltaic devices [15] and

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• ferrocene groups after the addition of antigen. One can conclude that ferrocene is useful as a transducing system to follow biosensing events but foreseeing the exact electrochemical behavior (amperometric vs potentiometric) remains very difficult. Furthermore, the redox center directly linked to the gold

• the specific analyte the current variation is weak denoting a very good specificity of the device. The current rise in the latter case is probably due to structural similarity between gIgG (i.e., antibody to hIgG antigen produced in goat) and the other immune proteins. Enzyme-based biosensing The

• fast electron-transfer rates from the redox moiety to the electrode surface. Furthermore, the ferrocene vicinity to the HRP enzyme is useful to constantly re-oxidize the enzyme FAD cofactor thus allowing the design of fast response biosensing devices endowed with a large dynamic range. The LoD and the

Template-controlled mineralization: Determining film granularity and structure by surface functionality patterns

• Nina J. Blumenstein,
• Jonathan Berson,
• Stefan Walheim,
• Petia Atanasova,
• Johannes Baier,
• Joachim Bill and
• Thomas Schimmel

Beilstein J. Nanotechnol. 2015, 6, 1763–1768, doi:10.3762/bjnano.6.180

• the site-selective mineralization of a semiconductor material, zinc oxide (ZnO), on a chemically patterned surface [3]. ZnO thin films are of special interest since they can be used for different applications such as solar cells [16], biosensing devices [17] and others [18]. By using a nearly

Thermal treatment of magnetite nanoparticles

• Beata Kalska-Szostko,
• Urszula Wykowska,
• Dariusz Satula and
• Per Nordblad

Beilstein J. Nanotechnol. 2015, 6, 1385–1396, doi:10.3762/bjnano.6.143

• their application in various fields. The list of possible applications encompasses biomedical engineering, MRI contrast agents, hyperthermia treatment, sensing and biosensing [2][3]. They are also very promising candidates for electrical-related applications, for example, energy and magnetic storage

Growth and morphological analysis of segmented AuAg alloy nanowires created by pulsed electrodeposition in ion-track etched membranes

• Ina Schubert,
• Loic Burr,
• Christina Trautmann and
• Maria Eugenia Toimil-Molares

Beilstein J. Nanotechnol. 2015, 6, 1272–1280, doi:10.3762/bjnano.6.131

• for surface enhanced Raman spectroscopy [38][41][42][43] and for biosensing [44]. Furthermore, such gaps with precisely controlled dimensions allow for the systematic investigation of multipole surface plasmon modes [39][45][46]. Because of their high electrical conductivity [47], Au and Ag metallic

Synergic combination of the sol–gel method with dip coating for plasmonic devices

• Cristiana Figus,
• Maddalena Patrini,
• Francesco Floris,
• Lucia Fornasari,
• Paola Pellacani,
• Gerardo Marchesini,
• Andrea Valsesia,
• Flavia Artizzu,
• Daniela Marongiu,
• Michele Saba,
• Franco Marabelli,
• Andrea Mura,
• Giovanni Bongiovanni and
• Francesco Quochi

Beilstein J. Nanotechnol. 2015, 6, 500–507, doi:10.3762/bjnano.6.52

• 10.3762/bjnano.6.52 Abstract Biosensing technologies based on plasmonic nanostructures have recently attracted significant attention due to their small dimensions, low-cost and high sensitivity but are often limited in terms of affinity, selectivity and stability. Consequently, several methods have been

• at a metal–dielectric interface [1][2][3][4][5]. Recently, due to such remarkable properties, biosensing technologies based on plasmonic nanostructures have attracted significant attention, particularly in the development of label-free sensors [6][7][8]. It is well known that surface plasmons (SPs

• hydrophobic property of the surface is an important characteristic from the perspective of biosensing applications since it also allows for altering the surface affinity for specific molecules. The surface of the films prepared from fresh sol of pH ≈4 was further characterized through contact angle

Hollow plasmonic antennas for broadband SERS spectroscopy

• Gabriele C. Messina,
• Mario Malerba,
• Pierfrancesco Zilio,
• Ermanno Miele,
• Michele Dipalo,
• Lorenzo Ferrara and
• Francesco De Angelis

Beilstein J. Nanotechnol. 2015, 6, 492–498, doi:10.3762/bjnano.6.50

• large enhancement of the vibrational features both in the case of resonant excitation and out-of-resonance excitation. Such characteristics indicate that these structures are potential candidates for plasmonic enhancers in multifunctional opto-electronic biosensors. Keywords: biosensing; multiband

• has become an important factor for the routine application of this technique to biology. There has been a significant increase in the number of studies concerning applications based on plasmonic effects in recent years. The areas of interest range from biomedicine, with examples of biosensing devices

A scanning probe microscope for magnetoresistive cantilevers utilizing a nested scanner design for large-area scans

• Tobias Meier,
• Alexander Förste,
• Ali Tavassolizadeh,
• Karsten Rott,
• Dirk Meyners,
• Roland Gröger,
• Günter Reiss,
• Eckhard Quandt,
• Thomas Schimmel and
• Hendrik Hölscher

Beilstein J. Nanotechnol. 2015, 6, 451–461, doi:10.3762/bjnano.6.46

• effects; magnetostriction; scanning probe microscopes and components; Introduction Since its invention in the 1980s [1] the atomic force microscope (AFM) became a versatile tool frequently used in nanoscale metrology, biosensing, maskless lithography and high density data storage with nearly as many

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• ][45][54] and the construction of biosensing platforms [22]. Herein we present a feasible method to perform the postassembly bio-functionalization of amyloid fibrils. Our results demonstrate the robustness and stability of such amyloid. The resultant multicomponent nanostructure (nanoladders) should

Formation of stable Si–O–C submonolayers on hydrogen-terminated silicon(111) under low-temperature conditions

• Yit Lung Khung,
• Siti Hawa Ngalim,
• Andrea Scaccabarozzi and
• Dario Narducci

Beilstein J. Nanotechnol. 2015, 6, 19–26, doi:10.3762/bjnano.6.3

• order to gain access to the electronic properties of silicon, it is imperative that the organic layer on the top surface be kept thin enough to avoid a masking of the intrinsic properties of silicon, especially in biosensing application [1]. So far, hydrosilylation is among the most commonly accepted

Sequence-dependent electrical response of ssDNA-decorated carbon nanotube, field-effect transistors to dopamine

• Hari Krishna Salila Vijayalal Mohan,
• Jianing An and
• Lianxi Zheng

Beilstein J. Nanotechnol. 2014, 5, 2113–2121, doi:10.3762/bjnano.5.220

• electronic platform for biosensing [14]. Previously, numerous works have demonstrated that nucleic acid-functionalized CNT-based FETs exhibit fast, specific, and reproducible response to the detection of various polar molecules [15][16]. Since ssDNA has four bases, it gives the possibility of numerous

Effect of channel length on the electrical response of carbon nanotube field-effect transistors to deoxyribonucleic acid hybridization

• Hari Krishna Salila Vijayalal Mohan,
• Jianing An,
• Yani Zhang,
• Chee How Wong and
• Lianxi Zheng

Beilstein J. Nanotechnol. 2014, 5, 2081–2091, doi:10.3762/bjnano.5.217

• -dimensional electronic materials, and their further development has been encouraged in high frequency applications [8][9], chemical sensing [10][11] and biosensing [12][13]. Recently, SWCNTs have been demonstrated as one of the best biosensors for a number of reasons: i) their diameter (several nanometers) is

• characteristics, which are indicators of the various contributing FET biosensing mechanisms [19], vary with channel length. A change in more than one of these electrical parameters after hybridization indicates the influence of multiple FET mechanisms. Therefore, it becomes essential to take into consideration

High speed e-beam lithography for gold nanoarray fabrication and use in nanotechnology

• Jorge Trasobares,
• François Vaurette,
• Marc François,
• Hans Romijn,
• Jean-Louis Codron,
• Dominique Vuillaume,
• Didier Théron and
• Nicolas Clément

Beilstein J. Nanotechnol. 2014, 5, 1918–1925, doi:10.3762/bjnano.5.202

• after the self-assembly process. The developed technology, which will surely be of great importance for molecular electronics study on such nanoarrays, also promising exciting future works in chemistry and biosensing. Experimental Nanoarray fabrication As described in [1][8], for e-beam lithography, we