Search results
Search for "inhibition" in Full Text gives 148 result(s) in Beilstein Journal of Nanotechnology.
The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots
Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26
- carried out for 30 min at room temperature, cells were then washed and taken to a microscope. Inhibition of endocytosis MDCKII cells were pretreated with 25 μg/mL chlorpromazine [44], 30 μg/mL 5-(N,N-dimethyl)amiloride [45] and 1 μg/mL filipin III [46] (predissolved in dimethyl sulfoxide 0.5 μg/μL) upon
Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells
Beilstein J. Nanotechnol. 2015, 6, 167–176, doi:10.3762/bjnano.6.16
- experiments should be carried out with different cell lines and other well-defined nanoparticle species to elucidate possible general principles. Keywords: Caveolin-1; CDC42; endocytosis inhibition; iron oxide nanoparticles; nanoparticle uptake; Introduction Nanotechnology is expected to be a very powerful
- be of special interest for better control of particle properties. To obtain a deeper understanding of the signaling network underlying the uptake of SCIONs and SPIONs by tumor cells approaches like chemical inhibition of distinct endocytotic pathways, colocalization of particles with key-structures
SERS and DFT study of copper surfaces coated with corrosion inhibitor
Beilstein J. Nanotechnol. 2014, 5, 2489–2497, doi:10.3762/bjnano.5.258
- employed for such a purpose. A class of very efficient corrosion inhibitors for copper and its alloys in different media is formed by 1,2,4-triazole and its derivatives [1][2][3][4][5][6]. In particular, 1,2,4-triazole exhibits comparable corrosion inhibition [7] as compared to benzotriazole, which is much
- triazole (spectra D). Again, in this case, the spectrum does not show Raman bands due to the formation of cuprous oxide, which is related to the initial stage of the corrosion process of the metal. Therefore, the SERS findings highlight the corrosion inhibition effect by the layer of adsorbed triazole. To
- -transfer from molecule to metal with respect to the interaction with a neutral copper atom, about 0.5∙|e| versus 0.3∙|e|. Corrosion inhibition effect Considering the interaction of the molecule with the metal surface via the N4 atom, the possibility to form chains of adsorbed molecules linked by hydrogen
Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions
Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250
- , **p < 0.01. Inhibition of vacuolar ATPase by bafilomycin A1 antagonizes the toxic effect of PS-NH2 nanoparticles. (A) Analysis of lysosomal permeabilization of cells stimulated with PS-NH2 (100 µg/mL) with or without bafilomycin A1 (BFA, 6 nM) for 72 h. After treatment with nanoparticles, cells were
Nanobioarchitectures based on chlorophyll photopigment, artificial lipid bilayers and carbon nanotubes
Beilstein J. Nanotechnol. 2014, 5, 2316–2325, doi:10.3762/bjnano.5.240
- radical scavenging ability (affording an antioxidant activity of 73.25%) and enhanced biocidal ability, offering inhibition zones of 12.4, 11.3 and 10.2 mm in diameter, against Escherichia coli, Staphylococcus aureus and Enterococcus faecalis, respectively. Keywords: antibacterial activity; antioxidant
- (including the diameter of sample) on the agar surface around the sample with a minimum cut-off value set at 8 mm. The inhibition zone was measured and expressed in millimetres. In this study triplicate plates were prepared for each sample and bacterial strain. The mean zone of inhibition was calculated with
- ) showed weak antibacterial activity (see Figure 9), offering inhibition zone diameters in the range of 5.0–6.1 mm. However, their biohybrids with carbon nanotubes exhibited enhanced biocidal features due to the presence of SWCNTs, which are known to possess antimicrobial properties [47][48]. Kang et al
Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme
Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238
- PTX, and showed remarkable cancer growth inhibition after 48 h from I.P. injection. The increase rates of mean tumor volume in the PTX, saline, DDMC/PTX4, and DDMC/PTX5 groups were 1.85, 1.84, 1.39, and 1.53, respectively. Although PTX alone was ineffective, tumor volume for DDMC/PTX4 treatment fell
- the B16F10 melanoma cells, which are a well-known model of pulmonary metastasis. It is possible that the DDMC/PTX complex, which has the ability for angiogenesis prevention, growth inhibition, and suppression of fatal metastasis on B16F10 melanoma cells, is effective by these mechanisms. The efficacy
- [24]. Potential energy curve of paclitaxel (large) and DDMC/PTX complex (small) in the tumor inhibition reaction. Ea: activation energy of paclitaxel, Ea′: activation energy of the DDMC/PTX complex. Reprinted from [24]. In vivo analysis of the anticancer activity and survival rates of the DDMC/PTX
Effect of silver nanoparticles on human mesenchymal stem cell differentiation
Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214
- rapidly to biomolecules, such as DNA [27], negatively charged cell-wall components and the sulfhydryl groups of metabolic enzymes [7][28][29], which results in the inhibition of DNA-replication, an increase in membrane permeability and the disturbance of different metabolic pathways [30]. In contrast to
- . analyzed the expression of alkaline phosphatase in MSCs in the presence of Ag-NP (up to 1 μg·mL−1) after prolonged cell sulture (35 d). In contrast to the significant inhibition in cell proliferation observed at the highest concentration of Ag-NP, the authors found no influence on the activity of alkaline
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
- inhibition could be very interesting in the development of new anticancer therapies. Since all known drugs that are mutagenic are also capable of inducing SCE, we explored levels of SCE in K1 in cells treated with silver nanoparticles and untreated cells (Figure 13) and found a significant increase in the
Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages
Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174
- suggested that macropinocytosis and phagocytosis, as well as clathrin-mediated endocytosis, play a crucial role. The uptake of 40 nm nanoparticles in alveolar epithelial A549 cells was inhibited after depletion of cholesterol in the plasma membrane (preventing caveolin-mediated endocytosis) and inhibition
- ; endocytosis; inhibition; nanoparticles; uptake proteins; Introduction In recent years, the use of engineered nanoparticles (NPs) (defined as <100 nm in three dimensions according to ISO TS 27687:2008) has witnessed a strong rise in biomedical and pharmaceutical applications, specifically for targeted drug
- with specific inhibitors as a control to investigate clathrin-mediated endocytosis and fluorescent ctx-b with inhibitors as a control to analyze caveolin-mediated endocytosis. Polystyrene particles of 1 µm diameter were used to demonstrate the inhibition of phagocytosis. The uptake of fluorescently
Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity
Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158
- µg/mL and 32.68 µg/mL for Hela, which shows that these nanoparticles cause 50% inhibition of the cancer cell at a lower concentration in comparison to the fibroblasts. This can be attributed due to the difference in the surface charge at different pH conditions. The surface charge on BSA-AuNPs at
- was measured by using a microplate reader (FL600, Microplate Fluorescence Reader, Bio-Tek Company) at a wavelength of 570 nm. The concentration of the sample causing 50% inhibition of proliferation of the cells (IC50) was determined by plotting the percentage of cell viability versus the sample
Near-field photochemical and radiation-induced chemical fabrication of nanopatterns of a self-assembled silane monolayer
Beilstein J. Nanotechnol. 2014, 5, 1441–1449, doi:10.3762/bjnano.5.156
- patches disappeared. These findings indicate, that the small features of the gold mask do not lead to a complete inhibition of the destruction of the amino groups by the oxygen plasma. Chemically functional APTES SAM nanostructures were thus formed by the etching with oxygen plasma only at a resolution of
- and highly localized inhibition of the photochemical process induced by the UV–ozone treatment. Discussion The methods described here have great potential to generate nanopatterns as templates for the selective binding of molecules or nanoparticles with high spatial resolution. The high resolution
PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system
Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144
- binding to DNA, CPT antitumor activity is due to inhibition of the nuclear enzyme topoisomerase I [4][5]. In spite of its potential as chemotherapeutic agent, CPT suffers from a reduced in vivo antitumor efficacy owing to its poor water-solubility and chemical instability (Figure 1). CPT-derivatives with
Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone
Beilstein J. Nanotechnol. 2014, 5, 610–621, doi:10.3762/bjnano.5.72
- an inhibition of the differentiation of osteoclasts from their respective precursor cells (Figure 1B). In addition, after exposure of SaOS-2 cells to biosilica these cells increase the synthesis of the bone morphogenetic protein 2 (BMP2), a cytokine that induces osteoblast differentiation and
Mesoporous cerium oxide nanospheres for the visible-light driven photocatalytic degradation of dyes
Beilstein J. Nanotechnol. 2014, 5, 517–523, doi:10.3762/bjnano.5.60
- . Control experiments were performed in the absence of scavengers (black line, Figure 5a). The established scavengers used include sodium oxalate for h+ (red), CrO3 for e− (green), isopropanol for •OH (blue), and 1,4-benzoquinone for •OOH/•O2− (grey, Figure 5a) [5]. The inhibition of photocatalytic activity
Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating
Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35
- by using a cell culture incubator. The results indicated that the promotion of HSP70 was minimized during rapid heating. As mentioned before, ROS can activate the expression of HSP70. The inhibition of HSP70 during rapid-rate and low thermal dose heating could possibly mean the abolishment of ROS
- significantly impact cell growth. It is also noteworthy that NP concentrations equivalent to 5 µM IR820 had a slight cell growth inhibition effect on MES-SA cells. This is in line with our previous observations on the cytotoxicity effects of free IR820 on MES-SA, and seems to be related to the fact that drug
- (STATs) to the HSP70 promoters in vascular smooth muscle cells (VSMCs) [32]. This group exposed VSMCs to H2O2 and found that the cytoplasmic janus tyrosine kinase 2 (JAK2)/STAT pathway can up-regulate HSP70 and minimize oxidative stress effects on the cells. The inhibition of HSP70 expression under laser
3D-nanoarchitectured Pd/Ni catalysts prepared by atomic layer deposition for the electrooxidation of formic acid
Beilstein J. Nanotechnol. 2014, 5, 162–172, doi:10.3762/bjnano.5.16
- density of 0.26 A·mg−1 at −0.2 V because of the oxidation of HCOOH. A further increase of the potential leads to a decrease of the current density due to the oxidation of the palladium and the inhibition of the catalytic activity of the metallic system by reaction intermediates [9][41]. On the reverse
Some reflections on the understanding of the oxygen reduction reaction at Pt(111)
Beilstein J. Nanotechnol. 2013, 4, 956–967, doi:10.3762/bjnano.4.108
- similar decrease in current in this potential region during the reduction of H2O2 [25], suggest that adsorbed hydrogen on the electrode, Hads, may prevent the O–O bond cleavage and block reactive surface sites. Therefore only two electrons are exchanged in this potential range [17][23]. This inhibition by
New hybrid materials based on poly(ethyleneoxide)-grafted polysilazane by hydrosilylation and their anti-fouling activities
Beilstein J. Nanotechnol. 2013, 4, 671–677, doi:10.3762/bjnano.4.75
- shows the influence of the length of the grafted PEO and of the Si–H/allyl ratio on the bacterial adhesion in VNSS media. Compared to the unmodified PSZ coating, all PSZ-PEO surfaces exhibit lower levels of bacteria adhesion. The inhibition efficiency varies inversely with the ratio of Si–H/allyl. It is
- , there is 0.29 chains of allyl-PEO per ten Si–H groups of the PSZ chain. The bacterial adhesion inhibition depends on the grafting density and the chain length of PEO. The role of the grafting density is more important than that of the chain length. The PSZ-PEO350 coatings prepared at a ratio SiH/allyl
Functionalization of vertically aligned carbon nanotubes
Beilstein J. Nanotechnol. 2013, 4, 129–152, doi:10.3762/bjnano.4.14
- prevented, extending the catalyst lifetime [50]. Another positive effect of the use of the oxidant is the inhibition of Ostwald ripening if injected during the annealing treatment of the catalyst film; the Ostwald ripening causes the formation of large particles thus reducing the yield of the CNT synthesis
Paper modified with ZnO nanorods – antimicrobial studies
Beilstein J. Nanotechnol. 2012, 3, 684–691, doi:10.3762/bjnano.3.78
- bacteria Escherichia coli were found to be inhibited in an area that is 239% and 163% the area of the paper sample under different room lighting conditions, i.e., halogen and fluorescent lamp illumination, respectively. For Gram-positive bacteria Staphylococcus aureus the zones of inhibition surrounding
- measure of zones of inhibition (edge of the square inhibition zone in centimeters) around the paper squares of side 1.5 cm for different samples with ZnO nanorods. The antimicrobial activity in the dark is due to the slow release of Zn2+ ions arising from partial dissolution of ZnO in the moist
- inhibits the growth of microbes coming in contact with it but also prevents microbial growth in the area surrounding the paper [21]. The zones of inhibition around the paper samples are shown in Figure 2. In our experiments we observed comparable zones of inhibition for E. coli after incubation times of 24
The oriented and patterned growth of fluorescent metal–organic frameworks onto functionalized surfaces
Beilstein J. Nanotechnol. 2012, 3, 570–578, doi:10.3762/bjnano.3.66
- surface occurs indeed selectively on the MUDA-functionalized surface (square areas), while the HDT surface shows remarkable inhibition of [Zn2(adc)2(dabco)] nucleation. The AFM measurement (Figure 7b,c) shows more details of the SURMOF pattern. In contrast to the nonpatterned MTCA surface, the [Zn2(adc)2
Magnetic-Fe/Fe3O4-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages
Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51
- . Different concentrations of nanoparticles were taken up by double-stable Mo/Ma cells over 24 h; the nanoparticle-concentration ranged from 0 to 320 μg/mL MNP-SN38 in fresh medium. After 24 h, the inhibition of cell proliferation was measured by using the MTT assay (Figure 4). We found only 20% of inhibition
- of nanoparticles contained 0.427 mg of iron, indicating that this amount of iron would be high enough for alternating magnetic field hyperthermia in combination with chemotherapy [54]. The MTT assay indicated that 8 pg of iron can be easily loaded in each cell (20% inhibition of cell proliferation
- ) (Figure 4 and Figure 7). It is even possible to load 16 pg of iron in each cell (with 50% inhibition of cell proliferation) indicating that 2.1·10−15 mol of SN38 can be easily loaded in each delivery cell. To test the release of SN38 by the self-contained Tet-On Advanced system, Mo/Ma cells were plated in
Conducting composite materials from the biopolymer kappa-carrageenan and carbon nanotubes
Beilstein J. Nanotechnol. 2012, 3, 415–427, doi:10.3762/bjnano.3.48
- commonly referred to as E407 (European Union specification) as well as being approved by the US Food and Drug Administration as a direct food additive [40]. Recent demonstrations of other applications include their use in drug delivery for the inhibition of viral infections [41][42]. Glycerin (or glycerol
Other Beilstein-Institut Open Science Activities
