Defects and defect-mediated engineering of two-dimensional materials: challenges and open questions

• Arkady V. Krasheninnikov,
• Matthias Batzill,
• Anouar-Akacha Delenda,
• Marija Drndić,
• Chris Ewels,
• Katharina J. Franke,
• Mahdi Ghorbani-Asl,
• Alexander Holleitner,
• Ado Jorio,
• Ute Kaiser,
• Daria Kieczka,
• Hannu-Pekka Komsa,
• Jani Kotakoski,
• Manuel Längle,
• David Lamprecht,
• Yun Liu,
• Steven G. Louie,
• Janina Maultzsch,
• Thomas Michely,
• Katherine Milton,
• Anna Niggas,
• Hanako Okuno,
• Joshua A. Robinson,
• Marika Schleberger,
• Bruno Schuler,
• Alexander Shluger,
• Kazu Suenaga,
• Kristian S. Thygesen,
• Richard A. Wilhelm,
• E. Harriet Åhlgren and
• Carla Bittencourt

Beilstein J. Nanotechnol. 2026, 17, 454–488, doi:10.3762/bjnano.17.31

• 1200 K, vacancy clusters (dark spots of varying size) are found to be exclusively attached to the bright areas of the moiré. They form indeed a lattice with about 90% of the sites occupied. Transfer of such nanomeshes to a foreign substrate [157] makes it possible to explore their use in membrane

Nanocarrier-integrated multilayer films produced by 3D printing for improved skin adhesion and curcumin photostability

• Thayse Viana de Oliveira,
• Ana Paula Farias Leão,
• Júlia Leão,
• Cesar Liberato Petzhold and
• Ruy Carlos Ruver Beck

Beilstein J. Nanotechnol. 2026, 17, 440–453, doi:10.3762/bjnano.17.30

• hydrophilic membrane, Millipore®), corresponding to a 500-fold dilution, as shown in Equation 1. The zeta potential was determined by measuring electrophoretic mobility at 25 °C using the same instrument (Zetasizer® Nano ZS, ZEN 3600, Malvern Instruments, USA). Prior to analysis, the samples were diluted 500

• -fold in an aqueous 10 mmol/L NaCl solution and filtered through a 0.45 µm membrane (Millipore®). The pH of the formulations was measured without prior dilution at 25 °C using a calibrated potentiometer (VB-10, Denver Instrument, USA). where C2 is the concentration of the sample after dilution, C1 is

• extraction with acetonitrile for 20 min, followed by filtration through a 0.45 µm membrane (Millipore®) prior to HPLC analysis. All analytical procedures were performed under dark conditions to protect the samples from light. Encapsulation efficiency was determined using an ultrafiltration–centrifugation

Nanoinformatics: spanning scales, systems and solutions

• Iseult Lynch,
• Diego S. T. Martinez,
• Kunal Roy and
• Georgia Melagraki

Beilstein J. Nanotechnol. 2026, 17, 423–427, doi:10.3762/bjnano.17.28

• Roy constructed a quantitative structure–property relationship (QSPR) model with 132 metal oxide (MeOx) nanomaterials to understand the possible mechanisms of cell membrane damage and the role of zeta potential (a proxy for surface charge) in particular. The results showed that zeta potential, along

• with periodic-table-based descriptors such as an increase in oxygen count, electronegativity, and formation of a cationic charge, all influence cell membrane damage, and had the potential to influence oxidative damage through free radical accumulation, which could lead to changes in the survival rate

Biomimetic nanoparticles in cancer photodynamic therapy: a review of targeted delivery systems and therapeutic outcomes

• Valentina I. Gorbacheva,
• Alexey S. Grabovoy,
• Polina S. Marukhina,
• Anastasiia O. Syrocheva and
• Ekaterina P. Kolesova

Beilstein J. Nanotechnol. 2026, 17, 396–422, doi:10.3762/bjnano.17.27

• body’s immune response [48]. BNPs utilizing a cell membrane to coat a synthetic core were reported in 2011. These nanoparticles consisted of a poly(lactic-co-glycolic) acid (PLGA) core coated with an outer layer derived from red blood cell (RBC) membranes [49]. It was demonstrated that the presence of

• lysis is essential for the extraction of biomimetic membranes; the membrane composition is directly influenced by the severity of the lysis method. Common techniques include (i) ultrasound, that is, membrane rupture via pressure waves (20–50 kHz), requiring precise control to prevent heat damage [54

• rupture [53]. Lysis buffer selection (containing membrane disruptors and protease inhibitors) depends on cell type and target protein localization. For example, cancer cells and macrophages respond better to hypotonic protocols [57], and harsh homogenization suits cytomembrane protein extraction (above 30

Eco-efficient materials for agricultural crops based on a mineral rich in MOR- and HEU-type zeolites

• Esperanza Yamile de la Nuez-Pantoja,
• Inocente Rodríguez-Iznaga,
• Gerardo Rodríguez-Fuentes,
• Vitalii Petranovskii,
• Ariel Martínez García,
• José Juan Calvino Gámez and
• Daniel Goma Jiménez

Beilstein J. Nanotechnol. 2026, 17, 381–395, doi:10.3762/bjnano.17.26

• electron microscope was used; prior to measurements, samples were adhered to supports to coat them with a gold layer. For TEM analysis, samples were deposited on a 200-mesh copper grid with carbon membrane. Images were observed on a Talos F200X ThermoScientific microscope at 44000× magnification in S-MET

Polycatecholamine nanocoatings on stainless steel: the effect on attachment of human fibroblasts and platelets

• Paulina Trzaskowska,
• Ewa Rybak,
• Maciej Trzaskowski,
• Kamil Kopeć,
• Jakub Krzemiński,
• Rafał Podgórski,
• Hatice Genc,
• Mehtap Civelek and
• Iwona Cicha

Beilstein J. Nanotechnol. 2026, 17, 365–380, doi:10.3762/bjnano.17.25

• very well with the surface area covered by fibroblasts. The dependence was directly proportional at the beginning of culture, and inversely proportional after 7 d. Figure 10b shows the schematic presentation of the different effects of high-Rku and low-Rku profiles on cell membrane stretching. In line

• cell membrane is strongly stretched, which deteriorates subsequent spreading and firm attachment [28] (Figure 10b). We speculate that this effect was responsible for the detachment of fibroblasts observed on the SS-PDA 4 h coating variant in our study (see Supporting Information File 1, Figure S3). The

• . (a) Graphical explanation of high-Rku and low-Rku profiles. (b) Cellular membrane stretching on high-Rku (top) and low-Rku (bottom) surfaces. Figure 10a was redrawn from [45]. Figure 10b was redrawn from [28]. Contact angle (CA) values measured for SS and each type of coating. SD – standard deviation

Beam shaping techniques for pulsed laser ablation in liquids: Unlocking tunable control of nanoparticle synthesis in liquids

• Sergio Molina-Prados,
• Nadezhda M. Bulgakova,
• Alexander V. Bulgakov,
• Jesus Lancis,
• Gladys Mínguez Vega and
• Carlos Doñate-Buendia

Beilstein J. Nanotechnol. 2026, 17, 309–342, doi:10.3762/bjnano.17.22

• microlens arrays, cylindrical lenses, diffractive lenses, and flat optics, which provide fixed spatial beam profiles [102]. For dynamic beam control, spatial light modulators (SLMs) are employed, with liquid crystal modulators, membrane mirrors, and digital micromirror devices (DMDs) being the most employed

• flexibility for some applications. Membrane mirrors, in contrast, allow for complete control of the beam including intensity and full surface actuation. Nevertheless, their low number of actuators restrict the precision of the modulation, making them primarily useful for correcting optical aberrations

Durable antimicrobial activity of fabrics functionalized with zeolite ion-exchanged nanomaterials against Staphylococcus aureus and Escherichia coli

• Perla Sánchez-López,
• Kendra Ramirez Acosta,
• Sergio Fuentes Moyado,
• Ruben Dario Cadena-Nava and
• Elena Smolentseva

Beilstein J. Nanotechnol. 2026, 17, 262–274, doi:10.3762/bjnano.17.18

• example, CuO NPs with a size of 4.8 nm demonstrated better antibacterial activity than larger particles (7.8 nm) [16]. The small Cu NPs have a greater capacity to penetrate the cell membrane [17][18]. Zinc oxide NPs are well known for their photocatalytic properties. Also, recent studies have demonstrated

Comparative study on 3D morphologies of delignified, single tracheids and fibers of five wood species

• Helen Gorges,
• Felicitas von Usslar,
• Cordt Zollfrank,
• Silja Flenner,
• Imke Greving,
• Martin Müller,
• Clemens F. Schaber,
• Chuchu Li and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2026, 17, 239–250, doi:10.3762/bjnano.17.16

• part of the membrane (torus) that is slightly larger than the pit aperture [4][7]. Similar to other plant cells, fibers and tracheids have a primary wall (P) composed of cellulose, hemicelluloses, and pectin that are formed during cell growth [8][9]. Once fully grown, they develop two secondary walls

From shield to spear: Charge-reversible nanocarriers in overcoming cancer therapy barriers

• Madhuri Yeduvaka,
• Pooja Mittal,
• Ameer Boyalakuntla,
• Usman Bee Shaik,
• Himanshu Sharma,
• Thakur Gurjeet Singh,
• Siva Nageswara Rao Gajula and
• Lakshmi Vineela Nalla

Beilstein J. Nanotechnol. 2026, 17, 159–175, doi:10.3762/bjnano.17.10

• enhances cellular uptake by facilitating better interaction with the negatively charged cell membrane, improving drug delivery efficiency [45]. For example, nanoparticles engineered with pH-responsive polymers facilitate the controlled release of therapeutic agents specifically within the acidic tumour

Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery

• Dushko Shalabalija,
• Ljubica Mihailova,
• Nikola Geskovski,
• Andreas Zimmer,
• Otmar Geiss,
• Sabrina Gioria,
• Diletta Scaccabarozzi and
• Marija Glavas Dodov

Beilstein J. Nanotechnol. 2026, 17, 139–158, doi:10.3762/bjnano.17.9

• (Merck Group, Germany). CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS) and CytoTox-ONETM Homogeneous Membrane Integrity Assay (CytoTox) were obtained from Promega (Wisconsin, USA). Alexa FluorTM Phalloidin 488, Hoechst fluorescent stain and Dil stain (1,1'-dioctadecyl-3,3,3',3

• parabolic flow profile within the channel. This profile induces smaller particles to elute more swiftly compared to larger particles, particularly in proximity to the semipermeable membrane [22]. In particular, AF4 is a precise method for separating liposomes based on their hydrodynamic size, with particle

• , focusing rate and duration, sample loading, and carrier conditions. The composition of the carrier buffer, as well as its ionic strength and pH are crucial considerations for stabilizing the structures, preventing agglomeration or sedimentation, and avoiding interference with analytes and the membrane [23

Development and in vitro evaluation of liposomes and immunoliposomes containing 5-fluorouracil and R-phycoerythrin as a potential phototheranostic system for colorectal cancer

• Raissa Rodrigues Camelo,
• Vivianne Cortez Sombra Vandesmet,
• Octavio Vital Baccallini,
• José de Brito Vieira Neto,
• Thais da Silva Moreira,
• Luzia Kalyne Almeida Moreira Leal,
• Claudia Pessoa,
• Daniel Giuliano Cerri,
• Maria Vitória Lopes Badra Bentley,
• Josimar O. Eloy,
• Ivanildo José da Silva Júnior and
• Raquel Petrilli

Beilstein J. Nanotechnol. 2026, 17, 97–121, doi:10.3762/bjnano.17.7

• . These systems are formed by monoclonal antibodies linked to the lipid bilayer, allowing the nanoparticle to recognize and specifically bind to receptors overexpressed in the membrane of tumor cells. This active functionalization expands the therapeutic potential of liposomes by promoting greater

• cancer, but there are cellular mechanisms of resistance, such as membrane transporters, which lower the therapeutic response rate. However, when the treatment with 5-FU was evaluated, associated with photodynamic therapy using Foslip® as a liposomal system, it was found that this treatment was effective

• extracted R-PE (0.73) suggests the presence of other biomolecules – such as polysaccharides and residual proteins – which may further contribute to membrane stabilization during liposome formation [15]. For 5-FU, no significant difference was observed between the HSPC 25 and HSPC 50 formulations, which may

Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

• location for its absorption. Here, it attaches to a specific receptor (Cubam) on the ileal enterocyte membrane and is internalized into the enterocytes via endocytosis. Meanwhile, lysosomes break down IF, freeing VB12. This allows VB12 to bind to TC-II (HoloTC), facilitating its transport in the

• against ICP-MS results [112]. The CL potential of luminol for the determination of Co2+adsorbed on a chitosan membrane was assessed based on the catalytic effect of these ions on the luminol–H2O2 CL reaction, where the chitosan membrane provided the recognition surface, luminol acted as the indicator, and

• the detected CL light emission was the sensing output. Linear range and LOD of Co2+ were found to be 0.4 pg/L to 40 μg/L and 4.0 fg/L, respectively. The membrane/liquid CL system demonstrated analytical performance comparable to the liquid-only CL system. The pre-concentration of Co2+ on chitosan

Microplastic pollution in Himalayan lakes: assessment, risks, and sustainable remediation strategies

• Sameeksha Rawat,
• S. M. Tauseef and
• Madhuben Sharma

Beilstein J. Nanotechnol. 2025, 16, 2144–2167, doi:10.3762/bjnano.16.148

• water bodies. The simplest process to remove MPs from water supplies is by physical processes. Filtration processes, including sand filtration and membrane bioreactors (MBRs), utilize size exclusion to remove MPs [41]. Tiwari et al. established that specifically MBRs have been highly efficient, with

• them are new electrochemical technologies, membrane separation technologies, microbial technologies, and AOPs. The efficiency, mechanism, strengths, weaknesses, and applications of each technology are utilized to assess each technology. These technologies are indicative of the multidimensional

• treatment plants (WWTPs) are significant contributors to MP pollution, upgrading filtration technology is crucial. Germany and Switzerland have also enhanced their WWTPs to add more advanced membrane bioreactors and electrocoagulation systems to minimize MP discharge [136]. Implementation of microfiber

Quality by design optimization of microemulsions for topical delivery of Passiflora setacea seed oil

• Daniel T. Pereira,
• Douglas Dourado,
• Danielle T. Freire,
• Dayanne L. Porto,
• Cícero F. S. Aragão,
• Myla L. de Souza,
• Guilherme R. S. de Araujo,
• Ana Maria Costa,
• Wógenes N. Oliveira,
• Anne Sapin-Minet,
• Éverton N. Alencar and
• Eryvaldo Sócrates T. Egito

Beilstein J. Nanotechnol. 2025, 16, 2116–2131, doi:10.3762/bjnano.16.146

• Discussion Oil extraction and characterization OPS was extracted using the Soxhlet method with n-hexane as the solvent, yielding 30.5 ± 0.8% (w/w) relative to the initial seed mass. After extraction, the oil was dried using a rotary evaporator to remove residual solvent, filtered through a PTFE membrane

• 60 °C. Afterward, the solvent was removed using a rotary evaporator under reduced pressure at a water bath temperature of 40 °C. The obtained oil was then filtered through a 0.45 µm PTFE membrane and stored in amber glass bottles under refrigeration (4 °C) until use. The oil yield was calculated

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• nanoparticles with bacterial cells and the production of ROS, which causes DNA damage and denaturation of proteins close to the bacterial membrane, causes cell membrane damage [37][38]. In addition, the electrostatic force generated between the bacterial cells and the synthesized NCs causes distraction of the

• -dependent studies, larger nanoparticles tend to have higher MICs for E. coli, indicating that membrane permeability and nanoparticle size are key factors affecting antimicrobial strength [42]. Of particular interest is the high resistance exhibited by B. subtilis, which demonstrated an MIC of 5.0 mg/mL and

Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• membrane applications. Thermal and crystalline analyses demonstrated improved thermal stability upon NE-βCp incorporation. Collectively, these results provide robust evidence for the feasibility of producing multifunctional nanofiber membranes that successfully integrate a polymer–lipid hybrid core

• an interconnected nanoarchitecture, forming a membrane with porosity that enhances surface hydrophobicity and promotes strong adhesion forces with water [61]. In core–shell nanofibers, the use of PLA as the shell inherently implies that the nanofibers will exhibit a hydrophobic surface due to the

• high hydrophobicity of this polymer. In cases where not all the hydrophilic core content is fully coated by the shell, a reduction in membrane hydrophobicity is observed due to the presence of hydrophilic nanofibers [77]. Surface hydrophilicity assays revealed that the produced nanofibers exhibit high

The cement of the tube-dwelling polychaete Sabellaria alveolata: a complex composite adhesive material

• Emilie Duthoo,
• Aurélie Lambert,
• Pierre Becker,
• Carla Pugliese,
• Jean-Marc Baele,
• Arnaud Delfairière,
• Matthew J. Harrington and
• Patrick Flammang

Beilstein J. Nanotechnol. 2025, 16, 1998–2014, doi:10.3762/bjnano.16.138

• . In P. californica, the concentration of magnesium is sufficient to balance the negative charges of the phosphates [9]. This high magnesium concentration is indicative of the presence of an ATP-dependent H+/Mg+ antiporter in the granule membrane [9]. In S. alveolata, the heterogeneous granules also

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• ) can be considered for its much lower hydrophobicity compared to DBCO [57]. A study using TCO-tetrazine conjugation for ultrasound-guided membrane anchoring highlights the versatility of TCO-functionalized PEG lipids in mediating bioorthogonal conjugation. Successful incorporation of DOPE-PEG-TCO into

• deposition of complement fragments and formation of the membrane attack complex [64]. A study showed that exposure of LNPs containing 5 mol % DSPE-PEG2k to anti-PEG IgM and complement-active serum induced up to 50.5% release of encapsulated mRNA payload. In the widely used LNP composition containing 1.5 mol

Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties

• Laryssa Ferreira do Nascimento Silva,
• Douglas Dourado,
• Thayse Silva Medeiros,
• Mariana Alice Gonzaga Gabú,
• Maria Cecilia Queiroga dos Santos,
• Daiane Rodrigues dos Santos,
• Mylena Lemos dos Santos,
• Gabriel Bezerra Faierstein,
• Rosângela Maria Rodrigues Barbosa and
• Fabio Rocha Formiga

Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132

• conducted in a laboratory under controlled conditions, where samples of the nanoemulsion were applied to simulated human skin (using an artificial membrane) and exposed to previously fasted adult females of Ae. aegypti. The formulation, at a concentration of 200 µg/mL, provided a protection rate of 77.67

Current status of using adsorbent nanomaterials for removing microplastics from water supply systems: a mini review

• Nguyen Thi Nhan and
• Tran Le Luu

Beilstein J. Nanotechnol. 2025, 16, 1837–1850, doi:10.3762/bjnano.16.127

• ]. Recently, Yan et al. developed a reduced graphene oxide (S-rGO) membrane with small lateral size and a rejection rate of up to 99.9% while maintaining high water permeability (236.2 L·m−2·h−1·bar−1) [56]. As another type of material belonging to carbon-based adsorbents, CNTs have also gained attention

• ppm [64]. Based on the characteristics of MOFs, a MOF-covalent organic framework (COF) hybrid membrane (FS-50/COF(MATPA)-MOF(Zr)/PDA@PVDF) was constructed and achieved an MP removal rate of approximately 100%. This hybrid membrane was evaluated as a robust and environmentally friendly material [65

• certain level of MP removal depending on the specific treatment processes and operational conditions. To be specific, when applying the coagulation/sedimentation and membrane filtration processes, the concentration of MPs decreased by about 49.6% in raw water. In a WTP, the conventional treatment process

Phytol-loaded soybean oil nanoemulsion as a promising alternative against Leishmania amazonensis

• Victória Louise Pinto Freire,
• Mariana Farias Alves-Silva,
• Johny W. de Freitas Oliveira,
• Matheus de Freitas Fernandes-Pedrosa,
• Alianda Maira Cornélio,
• Marcelo de Souza-Silva,
• Thayse Silva Medeiros and
• Arnóbio Antônio da Silva Junior

Beilstein J. Nanotechnol. 2025, 16, 1826–1836, doi:10.3762/bjnano.16.126

• 1.65-fold decrease in the PHYT-NE IC50 values at 24 hours, and approximately a 5.6-fold decrease at 48 hours when compared to free-PHYT. A study conducted by da Silva et al. (2015) [17] demonstrated that the leishmanicidal activity of phytol is associated with induction of mitochondrial membrane

• parasite survival; therefore, to exert its effect, the compound must be taken up and accumulate in the cytosol [47]. In the free-living promastigote form, nanoemulsions may facilitate drug access to the intracellular target by more efficiently permeating the membrane of the parasite – either due to their

• nanoscale size, lipid fusion with membrane components, or disruption of the plasma membrane structure [48][49]. However, several studies have also highlighted the remarkable potential of nanoemulsions against intracellular amastigote forms. In addition to enhancing cutaneous penetration, nanoemulsions also

Electrical, photocatalytic, and sensory properties of graphene oxide and polyimide implanted with low- and medium-energy silver ions

• Josef Novák,
• Eva Štěpanovská,
• Petr Malinský,
• Vlastimil Mazánek,
• Jan Luxa,
• Ulrich Kentsch and
• Zdeněk Sofer

Beilstein J. Nanotechnol. 2025, 16, 1794–1811, doi:10.3762/bjnano.16.123

• ]. GO film was prepared through filtration using a polycarbonate membrane (Nucleopore 45 μm, 90 mm in diameter). The density of the resulting film was measured as 1.36 g·cm−2. An additional material incorporated in the study was polyimide (PI) with a density 1.42 g·cm−3 and a surface resistance above

Exploring the potential of polymers: advancements in oral nanocarrier technology

• Rousilândia de Araujo Silva,
• Igor Eduardo Silva Arruda,
• Luise Lopes Chaves,
• Mônica Felts de La Roca Soares and
• Jose Lamartine Soares Sobrinho

Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122

• solubility and permeability in the cell membrane both in vitro and in vivo, demonstrated the importance of these characteristics for the pharmacokinetic performance of drugs and developed the “Biopharmaceutical Classification System” (BCS), which remains widely used today by regulatory agencies, research

• opening of these intercellular tight junctions [75] as described in Table 2 and discussed in Section 4.1.1. M cells are characterized by invaginations in their membrane, which facilitate the selective entry of substances into the lymphatic tissue beneath the intestinal mucosa via active transport. This

• ]. Endocytosis is the primary pathway through which NPs enter cells. Hydrophobic polymeric NPs often exhibit stronger interactions with the lipid bilayer, which may facilitate their internalization through endocytic mechanisms. In contrast, hydrophilic NPs tend to adsorb onto the cell membrane surface before

Advances of aptamers in esophageal cancer diagnosis, treatment and drug delivery

• Yang Fei,
• Hui Xu,
• Chunwei Zhang,
• Jingjing Wang and
• Yong Jin

Beilstein J. Nanotechnol. 2025, 16, 1734–1750, doi:10.3762/bjnano.16.121

• development as a cost-effective, yet highly specific, molecular probe. Mass spectrometry analysis confirmed its target as a membrane-associated protein. Another study [57] showed that EpCAM was overexpressed in esophageal cancer samples, and the expression level of EpCAM was detected using antibodies or

• , which may be due to the fact that the aptamer was not modified and was easily degraded by nucleases. The cytotoxicity of Te4 to TE-1 and HEEC cells showed no potential as a therapeutic agent for EC alone. In addition, Te4 target has been identified as a membrane protein only by flow cytometry, and the

• aptamers, such as P42, P58, ZY3A and SPP1 aptamers, mostly exert pharmacological effects on the principle of target inhibition. ZY3A targets the membrane protein p37, and SPP1 belongs to secreted proteins. SOX2 and SOX2/CDP interfaces, the targets of P42 and P58, exist in cells, requiring aptamer