Modeling adsorption of brominated, chlorinated and mixed bromo/chloro-dibenzo-p-dioxins on C₆₀ fullerene using Nano-QSPR

• Piotr Urbaszek,
• Agnieszka Gajewicz,
• Celina Sikorska,
• Maciej Haranczyk and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 752–761, doi:10.3762/bjnano.8.78

• task is easiest for dioxins with symmetrically substituted halogen atoms, such as TCDD. Inside the cell it interacts with the AhR receptor, proteins, and finally, by entering the nucleus, it reacts with the so-called dioxin responsive element (DRE) region on the mRNA surface and causes errors in the

• translation process and synthesis of new proteins. Most studies are focused on chlorinated dioxins, but brominated dioxins can also be found in environmental samples [7]. Furthermore, in some cases, brominated dioxins show an even higher AhR receptor binding affinity than 2,3,7,8-TCDD [6][7][10]. Because of

• experimental studies aimed at interactions between fullerenes or other carbon nanomaterials with particles such as proteins [34], porphyrines [35], toxic water pollutants [14], solid phases [36], or other materials [37]. Dibenzo-p-dioxins and dibenzofurans produced during incineration of nanomaterials have

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• fluorescence as the ink below the feature surface also contributes to the signal. Protein and antibody binding onto lipid arrays The selective binding of proteins to the lipid arrays on HEMA polymer was first tested using fluorophore labelled streptavidin (STV-FITC) for the coupling with biotin headgroups

• still unknown, just like in case of lipids spotted on PVDF membranes [7], how the polymer mesh accommodates lipid geometry and packaging, and how it affects the accessibility of the target lipid regions for the binding of proteins and antibodies. However, the infiltrated lipid arrays show high stability

• differences between various protein complexes isolated from pathological samples from mice models and patient samples. Antibody based detection approaches could be suitable to analyze the levels of AR proteins bound onto the lipid arrays. The described model system would thereby have a significant impact on

Calculating free energies of organic molecules on insulating substrates

• Julian Gaberle,
• David Z. Gao and
• Alexander L. Shluger

Beilstein J. Nanotechnol. 2017, 8, 667–674, doi:10.3762/bjnano.8.71

• trajectories are finite and it is difficult to determine how long such simulations need to be. Attempts to compute free energies have been made for, i.e., proteins [32][33][34], ion solvation [35][36], small molecular clusters [37][38] and small molecules on surfaces [39][40][41]. While well-converged results

Dispersion of single-wall carbon nanotubes with supramolecular Congo red – properties of the complexes and mechanism of the interaction

• Anna Jagusiak,
• Barbara Piekarska,
• Tomasz Pańczyk,
• Małgorzata Jemioła-Rzemińska,
• Elżbieta Bielańska,
• Barbara Stopa,
• Grzegorz Zemanek,
• Janina Rybarska,
• Irena Roterman and
• Leszek Konieczny

Beilstein J. Nanotechnol. 2017, 8, 636–648, doi:10.3762/bjnano.8.68

• red shows unusual protein-binding properties – it preferentially interacts with partly unfolded beta-sheets and thus stabilizes unstable structural states that may emerge as a result of conformational changes linked with the biologic activity of some proteins (e.g., the alpha-1-proteinase inhibitor

• binding to carbon nanotubes but also for interaction with proteins (as it can adapt to the protein binding site) [31][34]. At solutions of low ionic strength, when CR supramolecular properties are much weaker, its interaction with SWNTs is greatly decreased. At higher ionic strength of the solution, which

The longstanding challenge of the nanocrystallization of 1,3,5-trinitroperhydro-1,3,5-triazine (RDX)

• Florent Pessina and
• Denis Spitzer

Beilstein J. Nanotechnol. 2017, 8, 452–466, doi:10.3762/bjnano.8.49

• insensitivity towards friction, but with the same impact sensitivity as conventional micrometer-sized RDX (Table 4). Reus et al. [45] then processed bicomponent systems: proteins and RDX/TNT. It is mentioned that the XRD patterns of the final products differ from that of the raw material, which seems to

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• polyethyleneimine (PEI; cationic nanoparticles) or carboxymethyl cellulose (CMC; anionic nanoparticles) and loaded with defined amounts of the fluorescently labelled proteins HTRA1, HTRA2, and BSA. The nanoparticles were purified by ultracentrifugation and characterized by dynamic light scattering and scanning

• electron microscopy. Various cell types (HeLa, MG-63, THP-1, and hMSC) were incubated with fluorescently labelled proteins alone or with protein-loaded cationic and anionic nanoparticles. The cellular uptake was followed by light and fluorescence microscopy, confocal laser scanning microscopy (CLSM), and

• flow cytometry. All proteins were readily transported into the cells by cationic calcium phosphate nanoparticles. Notably, only HTRA1 was able to penetrate the cell membrane of MG-63 cells in dissolved form. However, the application of endocytosis inhibitors revealed that the uptake pathway was

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• bioassay. We further evaluated non-specific binding by probing rabbit IgG and other non-target proteins. Figures S6–S9 (Supporting Information File 1) show the LSPR responses after the biochip was exposed to rabbit IgG (3 mg/mL), myoglobin (1 μg/mL), and cardiac troponin I (1 μg/mL). Consistent with the

• results in our previous work [17], the GNR sensor with antibodies thiolated with Traut’s reagent showed high specificity and the non-target proteins hardly caused a spectral shift. Similarly, the sensors resulting from the DTT, PEG6-CONHNH2, and SH-PEG-NH2/EDC modified antibodies did not probe rabbit IgG

Nanoscale isoindigo-carriers: self-assembly and tunable properties

• Tatiana N. Pashirova,
• Andrei V. Bogdanov,
• Lenar I. Musin,
• Julia K. Voronina,
• Irek R. Nizameev,
• Marsil K. Kadirov,
• Vladimir F. Mironov,
• Lucia Ya. Zakharova,
• Shamil K. Latypov and
• Oleg G. Sinyashin

Beilstein J. Nanotechnol. 2017, 8, 313–324, doi:10.3762/bjnano.8.34

• the treatment of leukaemia [28][29][30][31][32]. (iii) Another advantage of isatin and isoindigo derivatives is that they display binding properties of biomacromolecules (DNA, proteins and enzymes). It is noteworthy that isatin derivatives interact with DNA via an intercalating mechanism [12][13][33

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• single recognition reaction [6][8]. Luo and co-workers showed better sensitivities and shortened times for the detection of C-reactive proteins by using a quantum-dot-labeled immunoassay [13]. Accordingly, an improvement in sensitivity of 5,000 times for the detection of the ataxia telangiectasia mutated

• biomolecules [17][18]. Moreover, the AuNP–biomolecule binding can be completed by different procedures. Biomolecules can be simply adsorbed on the nanoparticle surface by means of electrostatic or hydrophobic interactions, leading to a high number of proteins per particle and random orientation of biomolecules

• , the strategy which demonstrated better sensitivity was used for detection of gliadin from wheat gluten, one of the main proteins of wheat gluten [24]. Gluten refers to a group of proteins contained in wheat, barley and rye and is thought to be the cause of celiac disease (CD). CD is an autoimmune

Impact of surface wettability on S-layer recrystallization: a real-time characterization by QCM-D

• Jagoba Iturri,
• Ana C. Vianna,
• Alberto Moreno-Cencerrado,
• Dietmar Pum,
• Uwe B. Sleytr and
• José Luis Toca-Herrera

Beilstein J. Nanotechnol. 2017, 8, 91–98, doi:10.3762/bjnano.8.10

• isolated SbpA bacterial surface proteins onto silicon dioxide substrates of different surface wettability. Surface modification by UV/ozone oxidation or by vapor deposition of 1H,1H,2H,2H-perfluorododecyltrichlorosilane yielded hydrophilic or hydrophobic samples, respectively. Time evolution of frequency

• : bacterial S-layers; Quartz crystal microbalance with dissipation monitoring (QCM-D); recrystallization kinetics; surface wettability; Introduction Crystalline bacterial protein layers (S-layers) are arrays of (glyco)proteins (Mw of 40 to 200 kDa) forming the outermost envelope of prokaryotes, and represent

• obtained. The importance of such factors for the morphology and biological function of isolated proteins has been already shown in literature [16]. In this regard, the quartz crystal microbalance with dissipation monitoring (QCM-D) has proven to be a powerful technique to follow in situ the binding of S

Structural and tribometric characterization of biomimetically inspired synthetic "insect adhesives"

• Matthias W. Speidel,
• Malte Kleemeier,
• Andreas Hartwig,
• Klaus Rischka,
• Angelika Ellermann,
• Rolf Daniels and
• Oliver Betz

Beilstein J. Nanotechnol. 2017, 8, 45–63, doi:10.3762/bjnano.8.6

• have revealed that they form heterogeneous (emulsion-like) mixtures of aliphatic lipids, carbohydrates and proteins [4][15][16][17][18][19]. Adhesive secretions may form both oil-in-water (o/w) [20][21][22] or water-in-oil (w/o) emulsions [14][23]. Possible functional advantages lie in (i) their

• performed analyses [15][18] have confirmed the presence of polysaccharides, peptides and (glycosylated) proteins in the adhesive secretion of the desert locust Schistocerca gregaria and the Madagascan hissing cockroach Gromphadorhina portentosa and have thus confirmed previous assumptions of Vötsch et al

• their phase volume ratios and droplet-size distributions. Their adhesive and frictional performances were determined by nanotribometric measurements. We thus aimed at clarifying the influence of the various chemical compounds such as lipids, proteins and carbohydrates on the tribometric and rheological

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

• Roberta D'Agata,
• Pasquale Palladino and
• Giuseppe Spoto

Beilstein J. Nanotechnol. 2017, 8, 1–11, doi:10.3762/bjnano.8.1

• local aggregation of AuNPs that could be exploited to increase the sensitivity of AuNP-enhanced nucleic acid detection assays. Results and Discussion Functionalization of nanoparticles The adsorption of proteins on nanoparticles has been widely investigated over the last decade [26]. In particular, it

• has been demonstrated that the interaction between proteins and citrate-stabilized AuNPs occurs through a mechanism involving carboxylate–ammonium interactions established between citrate and lysine or histidine amino groups on the protein surface. The mechanism also includes contributions from steric

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

• Huijuan Zhang,
• Fuqiang Wu,
• Yazhen Li,
• Xiping Yang,
• Jiamei Huang,
• Tingting Lv,
• Yingying Zhang,
• Jianzhong Chen,
• Haijun Chen,
• Yu Gao,
• Guannan Liu and
• Lee Jia

Beilstein J. Nanotechnol. 2016, 7, 1861–1870, doi:10.3762/bjnano.7.178

• reactive amino side groups, chitosan could be made available via chemical modifications or ionic interactions [14]. Chitosan-bearing protonated amino groups could interact with a wide variety of natural or synthetic anionic species, such as negatively charged proteins, DNA [15][16][17][18][19], and some

Effective intercalation of zein into Na-montmorillonite: role of the protein components and use of the developed biointerfaces

• Ana C. S. Alcântara,
• Margarita Darder,
• Pilar Aranda and
• Eduardo Ruiz-Hitzky

Beilstein J. Nanotechnol. 2016, 7, 1772–1782, doi:10.3762/bjnano.7.170

• ]. Particularly, biological species can be employed in the preparation of these materials, giving rise to biohybrids, which represent a growing field of research addressed to produce advanced functional materials [2]. Many studies have demonstrated that even large molecules, such as polypeptides and proteins

• intercalation of gelatin into montmorillonite [4], other biohybrids also based on the assembly of smectite clays and proteins (e.g., bovine serum albumin, gelatin, casein or soy) have been vastly studied [5][6][7][8][9][10]. However, protein adsorption on montmorillonite clay can be considered a complex process

• interactions that can occur in less-studied proteins. Zein is the major storage protein of corn and an important source of protein in the human diet either through direct consumption or through consumption of animals whose feed is based on corn, such as poultry or swines [13]. Although zein is known since 1821

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• size of adhesion proteins and their ligands, or on the size of cells, the effective structuring of surfaces with nanometer to micrometer precision is required. In this section, we review the currently available most common techniques and materials applied for the fabrication of appropriate micro

• obtain nanofibers from natural proteins such as fibronectin. Recently, an alternative method has been developed where nanofibers of extruded fibronectin through a nanoporous aluminum oxide membrane were obtained. This method is based on a mechanical force to provoke fibrillogenesis (generation of fibers

• in its surface chemistry since biological cell adhesion via integrins or other adhesion molecules will generally not directly occur to inorganic or organic polymeric materials. Thus, further modification of the surface with adhesive molecules, for example with proteins from the extra cellular matrix

Polystyrene-block-poly(ethylene oxide) copolymers as templates for stacked, spherical large-mesopore silica coatings: dependence of silica pore size on the PS/PEO ratio

• Roberto Nisticò,
• Giuliana Magnacca,
• Sushilkumar A. Jadhav and
• Dominique Scalarone

Beilstein J. Nanotechnol. 2016, 7, 1454–1460, doi:10.3762/bjnano.7.137

• ; mesoporous silica; soft templating; sol–gel; Introduction Mesoporous materials with large, tunable porosity are currently being investigated as selective molecular sieves, finding potential applications in many fields such as catalysis, encapsulation of proteins, filtration and separation of large molecules

False positives and false negatives measure less than 0.001% in labeling ssDNA with osmium tetroxide 2,2’-bipyridine

• Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 1434–1446, doi:10.3762/bjnano.7.135

• ), and the Ph29 connector channel have been investigated as single-molecule sensing devices for ssDNA, RNA, dsDNA, and proteins [11][12][13]. The concept of nanopore-based sequencing, patented in 1998 [14], is based on applying a potential across an open pore embedded within an insulating membrane that

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

• Claudia Messerschmidt,
• Daniel Hofmann,
• Anja Kroeger,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

• adsorption of proteins to the silica surface followed by some kind of flocculation process. The hydrodynamic radius of these agglomerates was in the order of 100 to 300 nm (Table 2, Figure 2A). Morphological examination of the NP–cell membrane interaction This directly raises the question, if the

• interaction of SiNPs with cellular membranes and membrane proteins severely influences cell function and integrity. Hence, we exemplarily incubated HeLa cells for as long as 24 h using high particle concentrations of 3400 µg·mL−1. The subsequent TEM examination showed only cell fragments for all three

• membrane of a HeLa cell (Figure 3). The agglomeration of silica NPs is driven by the adsorption of proteins to the NP surface [31] followed by a kind of flocculation (Supporting Information File 1, Figure S4 shows the adsorbed proteins on the NP surface). The SEM micrographs reveal that the cell membrane

Straightforward and robust synthesis of monodisperse surface-functionalized gold nanoclusters

• Silvia Varela-Aramburu,
• Richard Wirth,
• Chian-Hui Lai,
• Guillermo Orts-Gil and
• Peter H. Seeberger

Beilstein J. Nanotechnol. 2016, 7, 1278–1283, doi:10.3762/bjnano.7.118

• carbohydrates [13], proteins [14], antibodies [15] and DNA [16] are commonly used as multivalent materials for biological studies. Gold nanoparticles have been used in vivo as radiotracers [15][17], for targeted delivery [18] and, when functionalized with carboxylic acids, inhibit β-amyloid fibril growth

Functional diversity of resilin in Arthropoda

• Jan Michels,
• Esther Appel and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2016, 7, 1241–1259, doi:10.3762/bjnano.7.115

• molecular prerequisites, resilin features exceptional rubber-like properties including a relatively low stiffness, a rather pronounced long-range deformability and a nearly perfect elastic recovery. Within the exoskeleton structures, resilin commonly forms composites together with other proteins and/or

• outstanding properties. Keywords: biological materials; biomechanics; composites; elastomeric proteins; functional morphology; Review Resilin – the pliant protein Elastomeric proteins occur in a large range of organisms and biological structures, and the spectrum of their biological functions is very broad

• [1]. They feature a great diversity including well-known examples such as elastin, titin and fibrillin present in vertebrate muscles and connective tissues, byssus and abductin of bivalve molluscs and gluten of wheat [1]. Besides spider silk proteins, resilin is certainly the best-known among the

Tunable longitudinal modes in extended silver nanoparticle assemblies

• Serene S. Bayram,
• Klas Lindfors and
• Amy Szuchmacher Blum

Beilstein J. Nanotechnol. 2016, 7, 1219–1228, doi:10.3762/bjnano.7.113

• [5], DNA [6], and proteins [7], including virus coat proteins [8][9] as well as rigid templates such as carbon nanotubes [10] have been extensively implemented and programmed for desired assemblies. In addition, assisted organization and alignment of nanoparticles via external directing magnetic [11

Preparation of alginate–chitosan–cyclodextrin micro- and nanoparticles loaded with anti-tuberculosis compounds

• Albert Ivancic,
• Fliur Macaev,
• Fatma Aksakal,
• Veaceslav Boldescu,
• Serghei Pogrebnoi and
• Gheorghe Duca

Beilstein J. Nanotechnol. 2016, 7, 1208–1218, doi:10.3762/bjnano.7.112

• active compound at the site of infection for a longer time, which consequently reduces the frequency of drug administration [10]; • interaction with plasma proteins [11], which has been shown to influence the biokinetics of the particles [12]. The main challenges in the usage of aerosols with

Reasons and remedies for the agglomeration of multilayered graphene and carbon nanotubes in polymers

• Rasheed Atif and
• Fawad Inam

Beilstein J. Nanotechnol. 2016, 7, 1174–1196, doi:10.3762/bjnano.7.109

• osteoblasts (bone-forming cells) and neurons, and found to be effective nano-carriers for several biomolecules such as proteins, DNA and carbohydrates [4]. Recently, MLG/CNT–polymer nanocomposites have been explored as scaffolds for cell growth and load-bearing implant materials for replacing defective human

High antiviral effect of TiO₂·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture

• Asya S. Levina,
• Marina N. Repkova,
• Elena V. Bessudnova,
• Ekaterina I. Filippova,
• Natalia A. Mazurkova and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2016, 7, 1166–1173, doi:10.3762/bjnano.7.108

• (+)strand may indicate that the negative strand is attacked immediately after its release from the complex with the viral proteins (nucleoprotein and polymerases) before transcription into (+)mRNA and replication into complementary (+)cRNA. The most efficient DNA5 oligonucleotide directed to the 3

An ellipsometric approach towards the description of inhomogeneous polymer-based Langmuir layers

• Falko O. Rottke,
• Burkhard Schulz,
• Klaus Richau,
• Karl Kratz and
• Andreas Lendlein

Beilstein J. Nanotechnol. 2016, 7, 1156–1165, doi:10.3762/bjnano.7.107

• based layers [18], as well as for those consisting of phospholipids [11], polymers [19], and even proteins [20]. All of them are amphiphilic in character and nonabsorbing in the visible spectral range, similar to PPDL, making the latter an appropriate candidate to represent this group of compounds

• films. It is our expectation that achievement of a comparable or even a greater impact by ellipsometric mapping is possible for analysis of layers consisting of proteins, enzymes or other biomolecules. Furthermore, the vertical dimension of their monolayers may even exceed the ultrathin film limit