Multifrequency AFM integrating PeakForce tapping and higher eigenmodes for heterogeneous surface characterization

• Yanping Wei,
• Jiafeng Shen,
• Yirong Yao,
• Xuke Li,
• Ming Li and
• Peiling Ke

Beilstein J. Nanotechnol. 2025, 16, 2077–2085, doi:10.3762/bjnano.16.142

• [24]. For AFM characterization, an aqueous suspension was prepared from this pre-exfoliated powder at a concentration appropriate for AFM imaging, approximately 0.1 mg·mL−1. This suspension was treated with mild ultrasound (bath sonication, 15 min) to ensure thorough dispersion. Subsequently, a 20 μL

Laser ablation in liquids for shape-tailored synthesis of nanomaterials: status and challenges

• Natalie Tarasenka

Beilstein J. Nanotechnol. 2025, 16, 1963–1997, doi:10.3762/bjnano.16.137

• layered materials, resulting in 2D nanostructures. This approach is utilized for the formation of carbon-based and metal dichalcogenide nanosheets. Apart from gases, ions and molecules present in the solution can participate in the exfoliation as well as applied ultrasound. The application of ultrasound

• can be useful to control the pressure in laser-generated cavitation bubbles. In [61] ultrasound application during PLAL allowed the formation of bismuth nanosheets, while bismuth NPs formed without ultrasound action in the same experimental conditions of ns PLAL of a bismuth target in water. The

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• ) can be considered for its much lower hydrophobicity compared to DBCO [57]. A study using TCO-tetrazine conjugation for ultrasound-guided membrane anchoring highlights the versatility of TCO-functionalized PEG lipids in mediating bioorthogonal conjugation. Successful incorporation of DOPE-PEG-TCO into

Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties

• Laryssa Ferreira do Nascimento Silva,
• Douglas Dourado,
• Thayse Silva Medeiros,
• Mariana Alice Gonzaga Gabú,
• Maria Cecilia Queiroga dos Santos,
• Daiane Rodrigues dos Santos,
• Mylena Lemos dos Santos,
• Gabriel Bezerra Faierstein,
• Rosângela Maria Rodrigues Barbosa and
• Fabio Rocha Formiga

Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132

• trichomes, which are the secretory tissues present in different vegetative parts of plants, such as leaves, flowers, roots, fruits, or stems [67]. These can be obtained by distillation with water or steam, microwave or ultrasound assisted extraction, or from the epicarp of fruits by a mechanical process, or

• in thymol (54.22%), p-cymene (15.04%), and γ-terpinene (10.46%) [116][117]. The oil was extracted by hydrodistillation (1.8% yield), and the nanoemulsions were prepared with 5% of oil or thymol, 5% of Tween® 80, and 90% of water by ultrasound at 50 °C. The nanoemulsions presented average sizes of 65

• ultrasound, with a ratio of 1:0.5 (oil/surfactant, polysorbate 80) and Milli-Q water. It presented an average size of 52.18 nm (±4.53), a polydispersity index of 0.237 (±0.006), and thermal stability. In bioassays, third instar larvae exposed to concentrations between 5 and 150 ppm showed LC50 of 58.72 ppm

Piezoelectricity of layered double hydroxides: perspectives regarding piezocatalysis and nanogenerators

• Evgeniy S. Seliverstov,
• Evgeniya A. Tarasenko and
• Olga E. Lebedeva

Beilstein J. Nanotechnol. 2025, 16, 1812–1817, doi:10.3762/bjnano.16.124

• biocompatibility might exhibit a piezoelectric effect under ultrasound irradiation and generate superoxide anion radicals [5]. The results confirmed the potential of the selected LDH for application in novel piezocatalytic tumor therapy as an effective sonosensitizer. The obtained data indicated that the

• ultrasound exposure broke the inversion symmetry of Ni/Fe-LDH, causing positive and negative polarization charges to arise. The migration of created charges in opposite directions under external mechanical force caused an intrinsic electric field. The authors also hypothesized that LDHs, as some 2D layered

• the same time, our review also reveals methodological and interpretative weaknesses that complicate direct comparison of results. These weaknesses are: (1) Lack of unified metrology: In many reports, piezoelectrical properties are derived from indirect effects (enhanced degradation under ultrasound or

Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• was analyzed using transmission electron microscopy (TEM) with a MORGAGNI 268D (FEI Company, USA), operated at 80 kV. Before imaging, the samples were sonicated in an ultrasound bath for 15 min, and a drop of the suspension was placed onto a copper grid (200 mesh) coated with formvar/carbon. Excess

• the droplets and PdI values decreased, indicating improved uniformity. PdI values below 0.3 suggest greater size homogeneity [79]. Under the same ultrasound emulsification conditions, the droplet diameter reaches an optimal point, indicating surfactant saturation at the droplet interface. This reduces

• through ultrasound [96]. The dilution process of the O/W(L-3%) system facilitated a more uniform distribution of lecithin molecules around the surface of the LO droplets, effectively reducing the interfacial tension. This process, coupled with low-energy ultrasound, allowed for the reduction of the

Prospects of nanotechnology and natural products for cancer and immunotherapy

• Jan Filipe Andrade Santos,
• Marcela Bernardes Brasileiro,
• Pamela Danielle Cavalcante Barreto,
• Ligiane Aranha Rocha and
• José Adão Carvalho Nascimento Júnior

Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116

• traditional therapies. Advances in cancer nanotechnology include the development of smart nanocarriers capable of responding to internal stimuli (such as pH, redox potential, and enzymes) and external stimuli (such as magnetic fields, heat, or ultrasound), enabling precise and controlled drug release [16][17

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• ]. Dellera et al. developed wound dressings containing silver sulfadiazine 7 and platelet lysate encapsulated in SLNs to treat persistent skin lesions. The Ag-encapsulating SLNs were prepared using ultrasound and hot homogenization techniques and incorporated into chitosan glutamate or hydroxypropylmethyl

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• immunoregulation using engineered liposomes targeting ferroptosis. While engineered liposomes facilitate ferroptosis induction, their therapeutic efficacy can be significantly enhanced through stimuli-responsive strategies and combination therapies. Stimuli such as pH, temperature, light, ultrasound, and ROS can

• , external stimuli (e.g., temperature, light, or ultrasound (US)) [172] can also be used. The release of drugs from liposomes stimulated by external stimuli offers greater precision in terms of timing and location of release as well as better regulation of drug delivery and dosage [172][173]. Figure 4 shows

Better together: biomimetic nanomedicines for high performance tumor therapy

• Imran Shair Mohammad,
• Gizem Kursunluoglu,
• Anup Kumar Patel,
• Hafiz Muhammad Ishaq,
• Cansu Umran Tunc,
• Dilek Kanarya,
• Mubashar Rehman,
• Omer Aydin and
• Yin Lifang

Beilstein J. Nanotechnol. 2025, 16, 1246–1276, doi:10.3762/bjnano.16.92

Hydrogels and nanogels: effectiveness in dermal applications

• Jéssica da Cruz Ludwig,
• Diana Fortkamp Grigoletto,
• Daniele Fernanda Renzi,
• Wolf-Rainer Abraham,
• Daniel de Paula and
• Najeh Maissar Khalil

Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90

• method was performed, followed by ultrasound for 5 minutes at 25 °C [95]. Xu et al. (2019) dissolved PVA in water at 90 °C, with separate addition of sodium alginate and glucomannan polysaccharide [140]. The freeze/thaw process was repeated three times. The cross-linking was done with saturated solutions

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• with ultrasound (US) exposure. The results showed that the NMs are not cytotoxic at the concentrations tested and the migration ability and calcium deposit formation of the cells treated with the NMs and upon US exposure were significantly increased. These results demonstrate that the hBNs have the

• stimulate electrically excitable cells without requiring an external power source, making them highly attractive for biomedical applications such as treating cardiac arrhythmias and promoting bone regeneration [17][18]. Mechanical stimulation, notably ultrasound (US), can induce a piezoelectric response in

• these materials [19]. In clinical practice, low-intensity pulsed ultrasound is commonly applied at intensity ranges between 20–50 mW/cm2 [20]. Combining piezoelectric materials including polymers such as polyvinylidene fluoride (PVDF) and inorganic materials such as barium titanate (BaTiO3) and

Supramolecular hydration structure of graphene-based hydrogels: density functional theory, green chemistry and interface application

• Hon Nhien Le,
• Duy Khanh Nguyen,
• Minh Triet Dang,
• Huyen Trinh Nguyen,
• Thi Bang Tam Dao,
• Trung Do Nguyen,
• Chi Nhan Ha Thuc and
• Van Hieu Le

Beilstein J. Nanotechnol. 2025, 16, 806–822, doi:10.3762/bjnano.16.61

• exhibits the aqueous dispersions derived from the sonication of GO-SG-ZH hydrogel and powder in water (see Supporting Information File 1, Figure S3). Ultrasound waves vibrated water molecules and created cavitation in the hydrogel structure, leading to the exfoliation of graphene-based nanosheets in water

Polyurethane/silk fibroin-based electrospun membranes for wound healing and skin substitute applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Muhammad Waqas,
• Ahsan Nazir and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 591–612, doi:10.3762/bjnano.16.46

• ultrasound to incorporate BP nanosheets for improved stability and processability. As a result, silk fibroin-doped black phosphorus nanosheets (BP@SF) were produced, which possess good water dispersity and long-term stability. BP@SF nanosheets were consolidated into fibrous membranes based on SF and PLGA by

Nanomaterials in targeting amyloid-β oligomers: current advances and future directions for Alzheimer's disease diagnosis and therapy

• Shiwani Randhawa,
• Trilok Chand Saini,
• Manik Bathla,
• Rahul Bhardwaj,
• Rubina Dhiman and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2025, 16, 561–580, doi:10.3762/bjnano.16.44

• cell-free therapeutic strategies for AD [84]. Additionally, Deng et al. explored exosomes isolated from ultrasound-stimulated human astrocytes (US-HA-Exo). Characterization via nanoparticle tracking and proteomic analysis revealed that ultrasound treatment increased exosome release approximately five

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

• undetectable levels after treatment. Employing a similarly galactosylated PLL system, Jing et al. explored a novel approach using Gal–PLL (Mw = 48 kDa, Gal/PLL = 24:1) to enhance the targeted delivery of c-myc antisense oligodeoxynucleotides to hepatocellular carcinoma (HCC) cells via ultrasound-targeted

Recent advances in photothermal nanomaterials for ophthalmic applications

• Jiayuan Zhuang,
• Linhui Jia,
• Chenghao Li,
• Rui Yang,
• Jiapeng Wang,
• Wen-an Wang,
• Heng Zhou and
• Xiangxia Luo

Beilstein J. Nanotechnol. 2025, 16, 195–215, doi:10.3762/bjnano.16.16

• , providing additional histological information. In addition to applying the photoacoustic effect to photoacoustic imaging, laser-induced focused ultrasound can also be used to perform high-precision cavitation ablation treatments of ocular tissues [208]. Conclusion Research on photothermal nanomaterials has

Liver-targeting iron oxide nanoparticles and their complexes with plant extracts for biocompatibility

• Shushanik A. Kazaryan,
• Seda A. Oganian,
• Gayane S. Vardanyan,
• Anatolie S. Sidorenko and
• Ashkhen A. Hovhannisyan

Beilstein J. Nanotechnol. 2024, 15, 1593–1602, doi:10.3762/bjnano.15.125

• described in [61]. For the preparation of extracts, the plant powder was mixed with the extragents, that is, 96%, 70% and 50% ethanol, diluted in phosphate buffer (pH 7.4) in a 1:30 (w/v) ratio and exposed to ultrasound at 75 W (ultrasonic homogenizer, Sonic-150W, MRC, Israel). After 24 h of incubation on

Nanotechnological approaches for efficient N2B delivery: from small-molecule drugs to biopharmaceuticals

• Selin Akpinar Adscheid,
• Akif E. Türeli,
• Nazende Günday-Türeli and
• Marc Schneider

Beilstein J. Nanotechnol. 2024, 15, 1400–1414, doi:10.3762/bjnano.15.113

• injection doses. However, these doses can also cause BBB disruption [139]. Researchers are working on the focused-ultrasound-mediated delivery of aducanumab by shortly opening the BBB, indicating the need for novel targeting strategies for mAbs [140]. As another example, ocrelizumab showed modest disease

Hymenoptera and biomimetic surfaces: insights and innovations

• Vinicius Marques Lopez,
• Carlo Polidori and
• Rhainer Guillermo Ferreira

Beilstein J. Nanotechnol. 2024, 15, 1333–1352, doi:10.3762/bjnano.15.107

• biological systems, it is possible to create innovative solutions in various fields, including communication, medical devices, and materials science. Certain hymenopterans are capable of emitting stridulatory sounds at high frequencies, including ultrasound (≥20 kHz) [179][180]. The design of medical devices

• could greatly benefit from innovations inspired by these stridulation mechanisms. Ultrasound technology has demonstrated significant potential in diagnostic imaging and ultrasound-responsive drug delivery [181][182]. Ultrasound technology is particularly promising for cancer treatment and disease

• effective ultrasound equipment, thus improving patient outcomes in both diagnostics and treatment. Furthermore, studying the use of acoustic signals in hymenopterans may offer valuable inspiration for future research, as these context-dependent signals can modulate the production or inhibition of chemical

Recent updates in applications of nanomedicine for the treatment of hepatic fibrosis

• Damai Ria Setyawati,
• Fransiska Christydira Sekaringtyas,
• Riyona Desvy Pratiwi,
• A’liyatur Rosyidah,
• Rohimmahtunnissa Azhar,
• Nunik Gustini,
• Gita Syahputra,
• Idah Rosidah,
• Etik Mardliyati,
• Tarwadi and
• Sjaikhurrizal El Muttaqien

Beilstein J. Nanotechnol. 2024, 15, 1105–1116, doi:10.3762/bjnano.15.89

• into the solid lipid NPs using the microemulsion-probe ultrasound method, while mannose 6-phosphate was conjugated to albumin. The mannose 6-phosphate-conjugated albumin was then decorated onto the surface of matrine-loaded solid lipid NPs, and its HSC-targeting efficiency was evaluated in vitro and in

Entry of nanoparticles into cells and tissues: status and challenges

• Kirsten Sandvig,
• Tore Geir Iversen and
• Tore Skotland

Beilstein J. Nanotechnol. 2024, 15, 1017–1029, doi:10.3762/bjnano.15.83

• imaging (i.e., positron emission tomography (PET), single-photon emission computed tomography (SPECT), optical/fluorescence, magnetic resonance imaging (MRI), computed tomography (CT; using X-ray) and ultrasound imaging), and the spatial resolution, depth of imaging, sensitivity, and advantages

Exfoliation of titanium nitride using a non-thermal plasma process

• Priscila Jussiane Zambiazi,
• Dolores Ribeiro Ricci Lazar,
• Larissa Otubo,
• Rodrigo Fernando Brambilla de Souza,
• Almir Oliveira Neto and
• Cecilia Chaves Guedes-Silva

Beilstein J. Nanotechnol. 2024, 15, 631–637, doi:10.3762/bjnano.15.53

• nitride as the initial material. In addition to using fewer reagents compared to conventional methods involving acidic baths, oxidizers, ultrasound, multiple reaction steps, exfoliation, and washes, the presented method operates in a single step [18][19]. In this study, we explore the exfoliation of TiN

Classification and application of metal-based nanoantioxidants in medicine and healthcare

• Nguyen Nhat Nam,
• Nguyen Khoi Song Tran,
• Tan Tai Nguyen,
• Nguyen Ngoc Trai,
• Nguyen Phuong Thuy,
• Hoang Dang Khoa Do,
• Nhu Hoa Thi Tran and
• Kieu The Loan Trinh

Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36

• delivery but also enhances stability and biocompatibility. Recently, Shang et al. used a Au/Cu1.6O/P–C3N5/Arg@HA nanocomposite hydrogel spray coupled with ultrasound for diabetic wound healing (Figure 4) [162]. This nanocomposite spray exhibited five types of enzyme-like activities, that is, CAT-, SOD