Computation-guided scaffold exploration of 2E,6E-1,10-trans/cis-eunicellanes

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1Department of Chemistry, University of Florida, PO Box 117200, Gainesville, FL 32611, USA
2Department of Chemistry, University of California–Davis, 1 Shields Ave., Davis, CA 95616, USA
3Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India
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Associate Editor: S. Bräse
Beilstein J. Org. Chem. 2024, 20, 1320–1326. https://doi.org/10.3762/bjoc.20.115
Received 03 Feb 2024, Accepted 21 May 2024, Published 07 Jun 2024
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Abstract

Eunicellane diterpenoids are a unique family of natural products containing a foundational 6/10-bicyclic framework and can be divided into two main classes, cis and trans, based on the configurations of their ring fusion at C1 and C10. Previous studies on two bacterial diterpene synthases, Bnd4 and AlbS, revealed that these enzymes form cis- and trans-eunicellane skeletons, respectively. Although the structures of these diterpenes only differed in their configuration at a single position, C1, they displayed distinct chemical and thermal reactivities. Here, we used a combination of quantum chemical calculations and chemical transformations to probe their intrinsic properties, which result in protonation-initiated cyclization, Cope rearrangement, and atropisomerism. Finally, we exploited the reactivity of the trans-eunicellane skeleton to generate a series of 6/6/6 gersemiane-type diterpenes via electrophilic cyclization.

Introduction

The eunicellane diterpenoids are a family of nearly 400 natural products that present a conserved 6/10-bicyclic hydrocarbon framework [1-3]. Mostly known from soft corals [2], but with a growing number of family members in bacteria and plants [4], these diterpenoids have four main structural differences: the number and location of oxidized carbons, the absence or presence of transannular ether bridges, the configuration (cis or trans) of the bicyclic ring fusion, and the presence and configuration (E or Z) of alkenes in the 10-membered ring (Figure 1A). During biosynthesis, the eunicellane skeleton is first constructed by terpene synthases that cyclize the diterpene precursor geranylgeranyl diphosphate (Figure 1B) [5-9]. The latter two structural elements, the configurations of the 6/10 system and the C2–C3 alkene, are instilled by these terpene synthases.

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Figure 1: Eunicellane diterpenoids and their biosyntheses. (A) The 6/10-bicyclic hydrocarbon framework is conserved in eunicellane diterpenoids. Selected natural products consisting of cis- (red) and trans-eunicellane skeletons (blue) are shown. (B) Four types of diterpene synthases are known to form the eunicellane skeleton, each with differing configurations at the ring-fused carbons and the 2,3-alkene.

Four types of eunicellane synthases are known (Figure 1B). The first eunicellane synthase identified, Bnd4 from the biosynthesis of benditerpenoic acid in Streptomyces sp. (CL12-4) [5], forms a cis-eunicellane named benditerpetriene (1) [6]. In 1, the C2–C3 and C6–C7 alkenes are E-configured, with the latter alkene configuration being conserved in all known eunicellane cyclization mechanisms. The first trans-eunicellane synthase, AlbS from the biosynthesis of albireticulone in Streptomyces albireticuli [10], was also identified from bacteria and forms albireticulene (2), a C1 diastereomer of 1 that also features the 2E alkene [7]. Two coral enzymes, BaTC-2 and EcTPS1, were found to form klysimplexin R (3), a 2Z-cis-eunicellane [8,9]. Recently, a third bacterial version, MicA, was identified as producing the 2Z-trans-eunicellane (4, microeunicellene) necessary for microeunicellol biosynthesis in Micromonospora sp. HM134 [11].

During our work on Bnd4, AlbS, and their enzymatic products 1 and 2, we noticed that the cis and trans-eunicellane skeletons behaved differently under certain conditions. For example, we successfully collected NMR data of 1 in chloroform [5], but when we dissolved 2 in chloroform for NMR, it cyclized into two 6/6/6-tricyclic diterpenes (5 and 6) [7]. We discovered that 2 was much more sensitive to acid than 1 and eventually took advantage of its reactivity to determine its absolute configuration [7]. Here, we sought to understand the molecular basis of chemical and thermal reactivities of these diterpene skeletons. We also took advantage of their intrinsic chemical properties to transform the eunicellanes in functionalized tricyclic skeletons of natural product importance.

Results and Discussion

Ring fusion configuration does not affect protonation-induced cyclization

Our initial observation that albireticulene (2) was unstable in chloroform, resulting in two tricyclic isomers gersemienes A (5) and B (6) [7], while benditerpetriene (1) was stable [5], led us to investigate the protonation-induced cyclization of the eunicellane skeleton (Figure 2). When 1 and 2 were individually subjected to acidic conditions (i.e., TFA in CHCl3), both cyclized into 6/6/6-tricyclic skeletons via a selective C2–C7 cyclization after protonation at C6 to give trans-BC ring systems (i.e., cis,trans-6/6/6 for 1 and trans,trans-6/6/6 for 2; Figure 2A). Only a single isomer, with the exocyclic olefin on ring C, of cis,trans-gersemiene was found, which matched a previous report of 1 cyclization in aqueous 0.1 M HCl into gersemiene C (7, Figures S1–S3, Supporting Information File 1) [12].

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Figure 2: Protonation-mediated cyclization of trans- and cis-eunicellanes. (A) The 2E-trans- and 2E-cis-eunicellane skeletons form 6/6/6-tricyclic gersemiene skeletons in the presence of acid. Epoxidation of 1 with mCPBA yields the epoxide 9, but the similar reaction with 2 yields gersemienol 8. Isolation yields are provided. (B and C) Results of DFT calculations on the protonation-induced cyclizations of 1 and 2. The energies of the cationic intermediates (italicized values) are not on the same energy scale as for the substrates and products (i.e., the free energy of B2+ is relative to A2+, while those of 5 and 6 are relative to that of 2; the free energy of B1+ is relative to A1+, while that of 7 is relative to 1; the energies given in B and C are not relative to each other). Note that the other rotamer of 7 is predicted to be 3.5 kcal mol−1 higher in energy than the one shown, an energy difference that is larger than expected based on experiment.

To assess these chemically-induced cationic cyclization mechanisms, we performed quantum chemical calculations [mPW1PW91/6–31+G(d/p)/SMD(chloroform)] [13-20] to obtain the relative free energies of the cationic intermediates and transition states that interconvert them, as well as the relative free energies of the neutral products. In the cyclization of 2, protonation at C6, results in a C7 tertiary cationic intermediate (A2+) where C2 is only 1.65 Å away from C7 (Figure 2B); this structure can be viewed as protonated 5 or 6 with a strongly hyperconjugated C2–C7 bond [21]. Reducing this hyperconjugation via bending the exocyclic methyl group such that the formal p-orbital at C3 no longer aligns with the C2–C7 bond results in the 6/6/6-tricyclic C3 tertiary cation B2+, which is 13.0 kcal mol−1 higher in energy. In principle, either A2+ or B2+ could be deprotonated to form products. A similar energy profile is seen for protonation-induced cyclization of 1, although the 6/6/6-tricyclic C3 tertiary cation is 7.1 kcal mol−1 higher in energy than its preceding intermediate (Figure 2C). A lower energy conformer of 1 exists, but we have been unable to find the corresponding cation A1b+, which implies that protonation and cyclization may be concerted for that conformer (Figure S4, Supporting Information File 1).

We previously transformed 2 into the trans,trans-6/6/6-tricyclic C6 alcohol 8 using mCPBA to epoxidize the C6–C7 alkene [7]. Under the same conditions, epoxidation of 1 yielded the 6,7-epoxide 9, which readily crystallized (Figure 2A and Figures S5–S10, Table S1 in Supporting Information File 1). Similarly, the 6,7-epoxy derivatives of klysimplexin R (3) and microeunicellene (4) were recently synthesized and isolated [11,21]; 3 cyclized to the 6/6/6-scaffold after the addition of acid [22]. An X-ray structure of 9 allowed us to solve its absolute configuration and measure the C2–C7 distance to be 3.31 Å. This distance matches reasonably well with the calculated distance, 3.36 Å, of the lowest energy conformer of 9. Calculation of the proposed 6,7-epoxy derivative of 2, which was never isolated, gave a similar estimated C2–C7 distance of 3.57 Å, suggesting that a factor other than distance controls any subsequent or concomitant cyclization.

Cope rearrangement is facile for trans-eunicellanes

During our study of the albireticulene (2) biosynthetic gene cluster, we found that 2 easily undergoes Cope rearrangement at 90 °C to generate the stereospecific 6/6-bicyclic product 10 in 96% yield (Figure 3A) [10]. This Cope rearrangement product was found as two inseparable atropisomers (10a/10b) at room temperature, which coalesced into a single conformer at 130 °C [10]. We anticipated that 1 would similarly undergo Cope rearrangement. However, when we heated 1 up to 200 °C for 5 h, we did not observe any Cope rearrangement products and we were able to recover >90% of 1. DFT calculations [mPW1PW91/6–31+G(d/p)/SMD(toluene)] on the Cope rearrangement of 2 revealed a free energy barrier through the chair–chair transition state of 28.1 kcal mol−1 and an overall ΔG from 2 to 10a/10b, which have the same predicted energy, of −2.0 kcal mol−1 (Figure 3B). The relative free energy barrier through the chair–boat transition state was calculated to be 36.9 kcal mol−1, but would require a prior chair-to-boat conformational change of 9.7 kcal mol−1, providing a similar activation barrier for the Cope rearrangement step itself (27.2 kcal mol−1). For 1, a potential Cope rearrangement was predicted to be 32.5 kcal mol−1, an overall higher energetic barrier compared to 2 (Figure S11, Supporting Information File 1). The lowest transition state for a Cope rearrangement, a chair–chair structure at 29.3 kcal mol−1, originates from a DD (down–down orientations of the methyl groups on the 10-membered ring) conformer that is 3.2 kcal mol−1 higher in free energy than the most dominant conformer, leading to the predicted overall barrier of 32.5 kcal mol−1 and consistent with the diminished reactivity observed experimentally. The two lowest energy conformers of 1 (−3.2 and −3.0 kcal mol−1 relative to the DD conformer mostly likely to undergo Cope rearrangement) would both require significantly higher free energy barriers of 38.0 and 38.8 kcal mol−1, respectively, to react through their associated chair–boat Cope transition states (Figure S11, Supporting Information File 1). In addition, the resulting products were predicted to be approximately 10 kcal mol−1 higher in free energies than the reactants. We note here, circumstantially, that no Cope rearrangement products of cis-eunicellanes have been reported. Overall, the trans ring fusion of the 2E,6E-trans-eunicellanes appears to control whether Cope rearrangement is energetically possible.

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Figure 3: Cope rearrangement in trans-eunicellanes. (A) The 2E-trans-eunicellane undergoes thermal Cope rearrangement, yielding atropisomers 10a/10b; an isolation yield of 96% was previously reported [10]. A biosynthetically related analog is similarly proposed to undergo oxy-Cope rearrangement to yield 13a/13b. (B) Results of DFT calculations indicate that the Cope rearrangement of 2 is more favorable via a chair–chair transition state than a chair–boat transition state. (C) Results of DFT calculations on the atropisomers 10a/10b and the free energy barriers for clockwise (blue) and counter-clockwise (red) rotations.

In the same biosynthetic study [10], we also identified what appeared to be an oxy-Cope product (13a/13b), which would logically originate from a 5-hydroxyalbireticulene analog (11, Figure 3A). Because this product was isolated directly from the producing bacterium as inseparable atropisomers (i.e., no heat was applied), we speculated that a lower activation energy may allow for oxy-Cope rearrangement at 28 °C. DFT calculations (in toluene) of 5-hydroxyalbireticulene for both the pseudo-axial and pseudo-equatorial conformations of the C5 hydroxy moiety suggest that there is not a significant difference in the free energy barriers for the oxy-Cope rearrangement (27.7 kcal mol−1; Figure S12, Supporting Information File 1) of uncharged 11 compared to that of 2 (Figure 3B). The free energy barrier value for 11 is likely too high to be non-enzymatic at 28 °C and may at least require deprotonation of the hydroxy group or at least H-bonding with solvent [23].

In regard to the atropisomerism of 10a/10b, the two atropisomers are approximately equivalent in energy (0.5 kcal mol−1 difference) and require at least 17.4 kcal mol−1 to undergo conversion (Figure 3C). Interestingly, rotation in different directions requires barriers that differ by 2 kcal mol−1.

Scaffold exploration of the eunicellane skeleton

During protonation-induced cyclization of 1 and 2, the C6–C7 alkene showed higher nucleophilicity than either of the other two double bonds likely due to the unique conformation of the eunicellane skeleton. This selective reactivity was further supported when the C6–C7 alkene of 2 was oxidized by mCPBA oxidation, resulting in formation of the gersemianol derivative 8 [7]. Inspired by the reactivity of the trans-eunicellane skeleton, we conducted a series of chemical transformations to convert 2 into trans/trans-6/6/6 bicyclic skeletons with various functional groups at C6 (Figure 4). The goals were to evaluate the scope of electrophile-mediated cyclization and diversify the gersemiane skeleton for bioactivity assays. A structural similarity search of 5 and 6 revealed that several natural products, including the coral gersemiols [24] and plagicosin N from liverwort [25], possess a similar 6/6/6-skeleton and likely originate from a cis-eunicellane skeleton.

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Figure 4: Scaffold exploration of the 2E-trans-eunicellane skeleton.

We first independently tested 2 with halogen electrophiles NCS and NBS (1.2 equivalents). NCS and NBS provided the C6-monohalogenated 6/6/6-tricyclic products 14 and 15 in 78% and 74% yields (Figure 4 and Figures S13–S23 in Supporting Information File 1). The 1H NMR chemical shifts for H6 of 6-chlorogersemiene (14), 3.81 ppm (J = 12.1, 4.8 Hz), and 6-bromogersemiene (15), 4.03 ppm (J = 12.4, 4.7 Hz), supported their assignments. The large coupling constants (>10 Hz) of H6 support its axial position and therefore the equatorial nature of the halogen. These configurations were also verified by NOESY correlations between H6 and H2 in both compounds. Some minor isomers of the halogenated derivatives were detected by LC–MS, but were deemed too low in abundance to purify. Two gersemiane derivatives (16 and 17), in a ratio of 3:1 in 82% overall yield, with a phenylselenyl groups at C6 were obtained when 2 was reacted with PhSeBr in acetonitrile overnight (Figure 4 and Figures S24–S36 in Supporting Information File 1). A similar reaction with PhSCl was also tested but yielded only several minor products and mostly starting material, hence we decided these products were not worth further purification. The minor isomers of the halogenated derivatives were presumably detected but in too low yields to purify. Since 6/6/6-tricyclic diterpenes are known to show cytotoxicity [26], we tested our seven analogs for cytotoxicity against human colon carcinoma HCT-116 cells. Unfortunately, none of the compounds up to 10 μM showed any activity when tested in MTT-based cell viability assays.

Conclusion

Eunicellane diterpenoids have been known for over 50 years, but it was not until recently that their biosynthetic precursors, the eunicellane skeletons, were identified and isolated. Currently, four different eunicellane skeletons are known: 2E-cis (1), 2E- trans (2), 2Z-cis (3), and 2Z-trans (4). Based on the current distribution of eunicellane diterpenoids isolated, the coral-derived 2Z-cis-eunicellane appears to be the dominant form in nature (≈98%). Perhaps the inherent reactivity seen for 2E-trans-eunicellanes contributes to their presumed rarity in nature, as certain conditions or transformations, either enzymatic or spontaneous, may alter the hydrocarbon skeleton. This idea is supported by the recent discovery of the aridacins, which transforms a 2E-cis-eunicellane into a 6/7/5-tricyclic diterpene via a cytochrome P450 [12]. Coincidentally, these reactive skeletons also provide chemists the ability to access synthetically challenging scaffolds with simple electrophilic cyclizations. With genome mining of bacterial eunicellane synthases suggesting there are a significant number of both cis- and trans-eunicellane diterpenoids remaining to be discovered [5,10-12], we expect continuing synthetic and biosynthetic advancements in eunicellane diterpenoids in the near future.

Supporting Information

Supporting Information File 1: Experimental methods, NMR and MS spectra, and crystallographic information.
Format: PDF Size: 6.5 MB Download

Acknowledgements

We acknowledge the University of Florida Center for Nuclear Magnetic Resonance Spectroscopy and Ion Ghiviriga for excellent NMR support. We acknowledge the University of Florida Mass Spectrometry Research and Education Center (MSREC), which is supported by the NIH (S10 OD021758-01A1), and Jodie Johnson for GC–MS support. We thank Wenbo Ning for measuring the melting point of 9.

Funding

This work was funded in part by NIH grant R35 GM142574 (to J.D.R.), NSF grant CHE-1856416 (to D.J.T.), and NSF CHE-2020110 (to S.L.). Computational resources were provided by NSF ACCESS program. S. J. was supported in part by the Institute of International Education (IIE) and the United States-India Education Foundation (USIEF) as part of a Fulbright Fellowship.

Data Availability Statement

All data that supports the findings of this study is available in the published article and/or the supporting information to this article. A data set collection of computational results is available in the ioChem-BD repository and can be accessed via https://doi.org/10.19061/iochem-bd-6-334. Crystal data for compound 9 was deposited into the CCDC, deposition number 2326275.

References

  1. Welford, A. J.; Collins, I. J. Nat. Prod. 2011, 74, 2318–2328. doi:10.1021/np200125v
    Return to citation in text: [1]
  2. Li, G.; Dickschat, J. S.; Guo, Y.-W. Nat. Prod. Rep. 2020, 37, 1367–1383. doi:10.1039/d0np00016g
    Return to citation in text: [1] [2]
  3. Dictionary of Natural Products. http://dnp.chemnetbase.com (accessed Dec 12, 2023).
    Return to citation in text: [1]
  4. Li, Z.; Rudolf, J. D. J. Ind. Microbiol. Biotechnol. 2023, 50, kuad027. doi:10.1093/jimb/kuad027
    Return to citation in text: [1]
  5. Zhu, C.; Xu, B.; Adpressa, D. A.; Rudolf, J. D.; Loesgen, S. Angew. Chem., Int. Ed. 2021, 60, 14163–14170. doi:10.1002/anie.202102453
    Return to citation in text: [1] [2] [3] [4] [5]
  6. Xu, B.; Tantillo, D. J.; Rudolf, J. D. Angew. Chem., Int. Ed. 2021, 60, 23159–23163. doi:10.1002/anie.202109641
    Return to citation in text: [1] [2]
  7. Li, Z.; Xu, B.; Kojasoy, V.; Ortega, T.; Adpressa, D. A.; Ning, W.; Wei, X.; Liu, J.; Tantillo, D. J.; Loesgen, S.; Rudolf, J. D. Chem 2023, 9, 698–708. doi:10.1016/j.chempr.2022.12.006
    Return to citation in text: [1] [2] [3] [4] [5] [6] [7]
  8. Scesa, P. D.; Lin, Z.; Schmidt, E. W. Nat. Chem. Biol. 2022, 18, 659–663. doi:10.1038/s41589-022-01027-1
    Return to citation in text: [1] [2]
  9. Burkhardt, I.; de Rond, T.; Chen, P. Y.-T.; Moore, B. S. Nat. Chem. Biol. 2022, 18, 664–669. doi:10.1038/s41589-022-01026-2
    Return to citation in text: [1] [2]
  10. Li, Z.; Xu, B.; Alsup, T. A.; Wei, X.; Ning, W.; Icenhour, D. G.; Ehrenberger, M. A.; Ghiviriga, I.; Giang, B.-D.; Rudolf, J. D. J. Am. Chem. Soc. 2023, 145, 22361–22365. doi:10.1021/jacs.3c09446
    Return to citation in text: [1] [2] [3] [4] [5] [6]
  11. Li, J.; Chen, B.; Fu, Z.; Mao, J.; Liu, L.; Chen, X.; Zheng, M.; Wang, C.-Y.; Wang, C.; Guo, Y.-W.; Xu, B. ChemRxiv 2023. doi:10.26434/chemrxiv-2023-jzr1p
    Return to citation in text: [1] [2] [3]
  12. Wang, Z.; Yang, Q.; He, J.; Li, H.; Pan, X.; Li, Z.; Xu, H.-M.; Rudolf, J. D.; Tantillo, D. J.; Dong, L.-B. Angew. Chem., Int. Ed. 2023, 62, e202312490. doi:10.1002/anie.202312490
    Return to citation in text: [1] [2] [3]
  13. Adamo, C.; Barone, V. J. Chem. Phys. 1998, 108, 664–675. doi:10.1063/1.475428
    Return to citation in text: [1]
  14. All structures were fully optimized (and confirmed as minima or transition state structures through vibrational analysis). Free energies were computed at this level of theory, which has been validated previously for terpene-forming carbocation cyclization/rearrangements; see, for example [15-18].
    Return to citation in text: [1]
  15. Tantillo, D. J. Exploring Terpenoid Biosynthesis With Quantum Chemical Computations. In Comprehensive Natural Products III: Chemistry and Biology; Liu, H.; Begley, T. P., Eds.; Elsevier: Amsterdam, Netherlands, 2020; pp 644–653. doi:10.1016/b978-0-12-409547-2.14656-6
    Return to citation in text: [1] [2]
  16. Matsuda, S. P. T.; Wilson, W. K.; Xiong, Q. Org. Biomol. Chem. 2006, 4, 530–543. doi:10.1039/b513599k
    Return to citation in text: [1] [2]
  17. Tantillo, D. J. Nat. Prod. Rep. 2011, 28, 1035–1053. doi:10.1039/c1np00006c
    Return to citation in text: [1] [2]
  18. Tantillo, D. J. Angew. Chem., Int. Ed. 2017, 56, 10040–10045. doi:10.1002/anie.201702363
    Return to citation in text: [1] [2]
  19. Gaussian 16, Revision C.01; Gaussian, Inc.: Wallingford, CT, 2016.
    Return to citation in text: [1]
  20. A data set collection of computational results is available in the ioChem-BD repository and can be accessed via https://doi.org/10.19061/iochem-bd-6-334. doi:10.19061/iochem-bd-6-334.
    Return to citation in text: [1]
  21. Tantillo, D. J. Chem. Soc. Rev. 2010, 39, 2847–2854. doi:10.1039/b917107j
    Return to citation in text: [1] [2]
  22. Scesa, P. D.; Schmidt, E. W. Angew. Chem., Int. Ed. 2023, 62, e202311406. doi:10.1002/anie.202311406
    Return to citation in text: [1]
  23. Schneider, C.; Weise, C. F. Cope, Oxy-Cope, and Anionic Oxy-Cope Rearrangements. Comprehensive Organic Synthesis, 2nd ed.; Elsevier: Amsterdam, Netherlands, 2014; Vol. 5, pp 867–911. doi:10.1016/b978-0-08-097742-3.00520-6
    Return to citation in text: [1]
  24. Angulo-Preckler, C.; Genta-Jouve, G.; Mahajan, N.; de la Cruz, M.; de Pedro, N.; Reyes, F.; Iken, K.; Avila, C.; Thomas, O. P. J. Nat. Prod. 2016, 79, 1132–1136. doi:10.1021/acs.jnatprod.6b00040
    Return to citation in text: [1]
  25. Qiao, Y.-N.; Jin, X.-Y.; Zhou, J.-C.; Zhang, J.-Z.; Chang, W.-Q.; Li, Y.; Chen, W.; Ren, Z.-J.; Zhang, C.-Y.; Yuan, S.-Z.; Lou, H.-X. J. Nat. Prod. 2020, 83, 1766–1777. doi:10.1021/acs.jnatprod.9b00895
    Return to citation in text: [1]
  26. Matias, D.; Nicolai, M.; Saraiva, L.; Pinheiro, R.; Faustino, C.; Diaz Lanza, A.; Pinto Reis, C.; Stankovic, T.; Dinic, J.; Pesic, M.; Rijo, P. ACS Omega 2019, 4, 8094–8103. doi:10.1021/acsomega.9b00512
    Return to citation in text: [1]
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