Volatile emission and biosynthesis in endophytic fungi colonizing black poplar leaves

• Christin Walther,
• Pamela Baumann,
• Katrin Luck,
• Beate Rothe,
• Peter H. W. Biedermann,
• Jonathan Gershenzon,
• Tobias G. Köllner and
• Sybille B. Unsicker

Beilstein J. Org. Chem. 2021, 17, 1698–1711, doi:10.3762/bjoc.17.118

• endophyte, a species of Cladosporium, we isolated and characterized two sesquiterpene synthases that can produce a number of mono- and sesquiterpenes like (E)-β-ocimene and (E)-β-caryophyllene, compounds that are dominant components of the herbivore-induced volatile bouquet of black poplar trees. As several

• -insect interactions. Keywords: Ascomycota; Cladosporium; Salicaceae; terpene synthases; volatile organic compound (VOC); Introduction Plant volatile organic compounds (VOCs) can mediate plant–insect, plant–microbe, and plant–plant interactions [1][2][3][4]. The constitutive and herbivore-induced

• , Cladosporium, Fusarium, Sordaria, and Stemphylium (Table 1). One unidentified species was also included in the volatile analysis. All the identified fungi belong to the Ascomycota, the largest fungal phylum. Endophytic fungi emit typical plant VOCs Altogether, we detected 77 volatile compounds in the

Isolation of fungi using the diffusion chamber device FIND technology

• Benjamin Libor,
• Henrik Harms,
• Stefan Kehraus,
• Ekaterina Egereva,
• Max Crüsemann and
• Gabriele M. König

Beilstein J. Org. Chem. 2019, 15, 2191–2203, doi:10.3762/bjoc.15.216

• litre of agar solution to obtain the required target concentration. Using this ratio, in our experiments, however, we had hardly any success (apart from one fungal isolate, i.e., Cladosporium allicinum and Heydenia cf. alpina from samples 2 and 3, respectively). Obviously in these experiments the

• resistant strains were isolated from soil but also not further processed. One isolate from experiment 4 was found to belong to the genus Cladosporium, and therefore was also not considered further. The remaining 36 isolates were identified at least to the genus level (Table 1). The pooled results from

• salt tolerance or dependency. Thus, we analysed the growth behaviour of Cladosporium allicinum and Heydenia cf. alpina on agar plates containing growth medium of increasing salinity from 0–35‰ (sea water has a salinity of approx. 35‰). As outcome the diameter of radial growth was measured after 14 days