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Search for "SELEX" in Full Text gives 7 result(s) in Beilstein Journal of Organic Chemistry.

Enhanced target cell specificity and uptake of lipid nanoparticles using RNA aptamers and peptides

  • Roslyn M. Ray,
  • Anders Højgaard Hansen,
  • Maria Taskova,
  • Bernhard Jandl,
  • Jonas Hansen,
  • Citra Soemardy,
  • Kevin V. Morris and
  • Kira Astakhova

Beilstein J. Org. Chem. 2021, 17, 891–907, doi:10.3762/bjoc.17.75

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  • exponential enrichment (SELEX) [30]. SELEX is an iterative process that begins with a large oligonucleotide library that, through a process of negative and positive selections, ends with a few candidates that are specific for a particular protein [30][31]. Using HIV-1 as our model, we explored the use of two
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Published 26 Apr 2021

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

  • Ekaterina A. Bryushkova,
  • Erik R. Gandalipov and
  • Julia V. Nuzhina

Beilstein J. Org. Chem. 2020, 16, 1135–1141, doi:10.3762/bjoc.16.100

Graphical Abstract
  • random sequence molecules, also known as SELEX (systematic evolution of ligands by exponential enrichment), and was a holistic harpin [22]. In further work MGA was separated into two strands, and nucleic acid binding arms were added to each strand, allowing MGA to target a sequence of interest
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Published 27 May 2020

Towards open-ended evolution in self-replicating molecular systems

  • Herman Duim and
  • Sijbren Otto

Beilstein J. Org. Chem. 2017, 13, 1189–1203, doi:10.3762/bjoc.13.118

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  • amplification and selection processes using a technique called systematic evolution of ligands by exponential enrichment, or SELEX. In SELEX, a library of DNA and RNA sequences is exposed to a certain target. In multiple selection rounds the binding species are selected and amplified, while the non-binding DNA
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Published 21 Jun 2017

Learning from the unexpected in life and DNA self-assembly

  • Jennifer M. Heemstra

Beilstein J. Org. Chem. 2015, 11, 2713–2720, doi:10.3762/bjoc.11.292

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  • be a privileged structure for the engineering of aptamers into split aptamers, as it offers two putative splitting sites that are distant from the typical target binding site (Figure 4a). Excitingly, Stojanovic and co-workers had recently demonstrated that SELEX could be carried out using a
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Published 23 Dec 2015

DNA display of glycoconjugates to emulate oligomeric interactions of glycans

  • Alexandre Novoa and
  • Nicolas Winssinger

Beilstein J. Org. Chem. 2015, 11, 707–719, doi:10.3762/bjoc.11.81

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  • co-workers elegantly extended the utility of SELEX [30] to generate aptamers functionalized with glycans through CuAAC [31][32]. Their approach, termed SELMA (selection with modified aptamers), is a multistep procedure that allows screening, selection and amplification of DNA glycoconjugates (Scheme
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Published 11 May 2015

Versatile synthesis of amino acid functionalized nucleosides via a domino carboxamidation reaction

  • Vicky Gheerardijn,
  • Jos Van den Begin and
  • Annemieke Madder

Beilstein J. Org. Chem. 2014, 10, 2566–2572, doi:10.3762/bjoc.10.268

Graphical Abstract
  • catalysts, also called DNAzymes [15][16][17] and RNAzymes [10][16][18][19][20][21], respectively. Most catalytic nucleic acids are generated using the SELEX (Systematic Evolution of Ligands by EXponential enrichment) protocol [22][23][24][25] that generates a library of potential catalysts, which are then
  • screened for catalytic potential using natural substrates or transition state analogues. Although examples [26][27][28][29] exist illustrating the introduction of modified residues and by that the development of new catalysts, the SELEX protocol becomes more labor intensive and reproducibility of results
  • groups for later incorporation into nucleic acid duplexes via solid phase DNA synthesis [33]. Results and Discussion As mentioned earlier, a number of research groups have used the SELEX or in vitro selection protocol for the incorporation of amino acid-like modifications into DNA or RNA where there is
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Published 04 Nov 2014

Synthesis of a bifunctional cytidine derivative and its conjugation to RNA for in vitro selection of a cytidine deaminase ribozyme

  • Nico Rublack and
  • Sabine Müller

Beilstein J. Org. Chem. 2014, 10, 1906–1913, doi:10.3762/bjoc.10.198

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  • ; SELEX; Introduction Since the discovery of the first catalytic RNA in the ciliate Tetrahymena thermophilia in 1982 [1], a number of naturally occurring ribozymes have been described [2]. Whereas all of these natural ribozymes accelerate transesterifications or, as in the case of the ribosome, the
  • reaction would be a valuable addition to the repertoire of RNA activities with relevance to the RNA world theory. A very useful and often applied technique for the generation of catalytic RNA structures is a variation of the classical SELEX approach (Systematic Evolution of Ligands by EXponential
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Published 15 Aug 2014
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