Artificial bioconjugates with naturally occurring linkages: the use of phosphodiester

• Takao Shoji,
• Hiroki Fukutomi,
• Yohei Okada and
• Kazuhiro Chiba

Beilstein J. Org. Chem. 2018, 14, 1946–1955, doi:10.3762/bjoc.14.169

• ]. The supported trinucleotide 1 was prepared from the support in 83% yield over 8 steps (Scheme S1, Supporting Information File 1) and used as a model in combination with 5-(benzylmercapto)-1H-tetrazole (BMT) as an activator (Table 1). The reaction was found to be rather sensitive to the concentration

• , entry 4). When the activator was switched to tetrazole, the yield was slightly decreased (Table 1, entry 5), while dicyanoimidazole (DCI) was proven to be an inefficient option for the reaction (Table 1, entry 6). It should be noted that the 5’-activated supported trinucleotide 2 was stable throughout

• the work-up procedure routinely used for the ACSS-assisted liquid-phase method. Namely, the 5’-activated supported trinucleotide 2 was readily separated as a precipitate by the addition of acetonitrile, and washing the precipitate with acetonitrile simultaneously rinses away excess reagents to afford

Preparation of trinucleotide phosphoramidites as synthons for the synthesis of gene libraries

• Ruth Suchsland,
• Bettina Appel and
• Sabine Müller

Beilstein J. Org. Chem. 2018, 14, 397–406, doi:10.3762/bjoc.14.28

• of potentially successful candidates. This purpose is best achieved by the usage of trinucleotide synthons for codon-based gene synthesis. We here review the strategies for the preparation of fully protected trinucleotides, emphasizing more recent developments for their synthesis on solid phase and

• on soluble polymers, and their use as synthons in standard DNA synthesis. Keywords: gene library; protein engineering; soluble support; synthesis; trinucleotide; Introduction Protein engineering is a highly actual research area with a number of potential applications [1][2][3][4]. The construction

• size and degree of randomization can be restricted [13][14]. Nevertheless, although those methods and sophisticated variations of them [14][15][16][17] have improved library design and synthesis, full control over randomization is not possible. This can be achieved only by the usage of trinucleotide

Synthetic mRNA capping

• Fabian Muttach,
• Nils Muthmann and
• Andrea Rentmeister

Beilstein J. Org. Chem. 2017, 13, 2819–2832, doi:10.3762/bjoc.13.274

• deprotection with ammonia is not possible. An early example of capped RNA prepared by solid-phase synthesis was reported by the group of Sekine in 2001 [112]. A 2,2,7-trimethylguanosine (TMG)-capped trinucleotide block of U1 snRNA with the structure m32,2,7G5′pppAm2′Um2′A was prepared, starting from a 5

A journey in bioinspired supramolecular chemistry: from molecular tweezers to small molecules that target myotonic dystrophy

• Steven C. Zimmerman

Beilstein J. Org. Chem. 2016, 12, 125–138, doi:10.3762/bjoc.12.14

• bonds, hypothetically forming base-triplet with 2,4,6-triamino-1,3,5-triazine. (c) Aromatic recognition unit tethered to intercalator in stacked and unstacked conformation. (d) Ligand 27, an inhibitor of MBNL1N sequestration. (a) CTG trinucleotide repeat expansion in DMPK gene produces expanded