Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• arrangement of these two phases directly influences its degradation behavior. Studies have shown that PLA with higher crystallinity, in both film [73][74] and fiber forms [65], exhibits a higher rate of hydrolytic degradation in phosphate-buffered saline (PBS) at pH 7.4. This is attributed to the higher

The cement of the tube-dwelling polychaete Sabellaria alveolata: a complex composite adhesive material

• Emilie Duthoo,
• Aurélie Lambert,
• Pierre Becker,
• Carla Pugliese,
• Jean-Marc Baele,
• Arnaud Delfairière,
• Matthew J. Harrington and
• Patrick Flammang

Beilstein J. Nanotechnol. 2025, 16, 1998–2014, doi:10.3762/bjnano.16.138

• % paraformaldehyde in phosphate-buffered saline, rinsed, and air-dried. Worms were left in the remaining proximal part of the tube and were provided with glass beads (425–600 µm in diameter; Sigma) to reconstruct the missing part [25]. Scanning electron microscopy and elemental composition analyses For secondary

• % paraformaldehyde solution in phosphate-buffered saline (pH 7.4). The samples were then dehydrated through graded ethanol series and embedded in paraffin wax. Sections of 14 µm in thickness were cut with a Microm HM 340 E microtome and mounted on Superfrost Ultra Plus (Thermo Scientific) microscope slides using a

• suggested that secretion is accompanied by a jump in pH from 5 in the secretory granule to 8.2 in seawater that could trigger a change from electrostatic to ionic bonds between divalent cations and phosphate, the effect of which would be to harden spontaneously and solidify the hollow spheroids [19]. This

Self-assembly and adhesive properties of Pollicipes pollicipes barnacle cement protein cp19k: influence of pH and ionic strength

• Shrutika Sawant,
• Anne Marie Power and
• J. Gerard Wall

Beilstein J. Nanotechnol. 2025, 16, 1863–1872, doi:10.3762/bjnano.16.129

• achieved (Supporting Information File 1, Figure S1). Purified protein was dialysed into 10 mM sodium acetate (pH 4.0) or 10 mM sodium phosphate (pH 8.0) buffer, with NaCl concentrations ranging from 0 to 600 mM. Transmission electron microscopy TEM analysis identified the formation of fibril structures by

• . Formation of protein fibrils Purified rPpolcp19k-his protein was concentrated to 500 µg/mL using an Amicon® Ultra Centrifugal Filter, 10 kDa MWCO (Merck). The protein was reconstituted at 500 µg/mL in 10 mM sodium acetate buffer (pH 4.0) or 10 mM sodium phosphate buffer (pH 8.0) with NaCl concentrations

• rPpolcp19k-his protein (500 µg/mL), incubated for 0, 3, 11, or 21 days under varying pH and NaCl conditions, was applied to 200 mesh Cu formvar/carbon-coated grids (Agar Scientific) and allowed to settle for 5 min. Grids were washed three times in phosphate-buffered saline for 5 min each, followed by three

Exploring the potential of polymers: advancements in oral nanocarrier technology

• Rousilândia de Araujo Silva,
• Igor Eduardo Silva Arruda,
• Luise Lopes Chaves,
• Mônica Felts de La Roca Soares and
• Jose Lamartine Soares Sobrinho

Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122

Advances of aptamers in esophageal cancer diagnosis, treatment and drug delivery

• Yang Fei,
• Hui Xu,
• Chunwei Zhang,
• Jingjing Wang and
• Yong Jin

Beilstein J. Nanotechnol. 2025, 16, 1734–1750, doi:10.3762/bjnano.16.121

• , incorporating specific assessments for immunotoxicity, organ toxicity, and nanoparticle accumulation in organs. Second, the negatively charged phosphate backbone of nucleic acid aptamers generates electrostatic repulsion with the anionic phospholipid bilayer of cell membranes, significantly limiting their

Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• addition to PFAs, phospholipids are notable for their structure, which includes a hydrophilic part (phosphate groups) and a hydrophobic part (fatty acid chains). This unique configuration allows phospholipids to interact at the interface between the lipid and aqueous layers of the tear film, increasing its

• ), triton-X100 (code T8787), phosphate buffered saline (PBS, code P4417), and F.A.M.E. Mix (C8–C24 unsaturated, code 18918) were purchased from Merck®/Sigma-Aldrich® (Brazil). Hydrochloric acid (HCl, code R0101811000), sodium chloride (NaCl, code C1003460500), ethyl acetate (CH3COOCH2CH3, code R2800491000

• ), and ethanol (C2H5OH, code R0401701000) were purchased from CRQ Produtos Químicos. Disodium phosphate (Na2HPO4·2H2O, code P.10.0513.012.00.27), petroleum ether (code P.10.0450.000.04.81), sodium dodecyl sulfate (C12H25SO4Na, code P.10.0645.000.00.27) were purchased from Dinâmica Química Contemporânea

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• ). The gel was stained with Coomassie Brilliant Blue R-250 solution and scanned [48]. In vitro protein release To monitor the protein release profile, 1.5 mL of venom-loaded nanoparticles (0.1% PLA w/v, 1% venom w/w) were suspended in 1 mL of phosphate buffer solution (pH 7.4, KH2PO4 0.05 mol⋅L−1), in a

Transient electronics for sustainability: Emerging technologies and future directions

• Jae-Young Bae,
• Myung-Kyun Choi and
• Seung-Kyun Kang

Beilstein J. Nanotechnol. 2025, 16, 1545–1556, doi:10.3762/bjnano.16.109

• and the presence of specific ions, such as hydrogen phosphate and chloride (HPO42− and Cl−) [43] are known to accelerate the degradation process. The biodegradation rate of silicon is also influenced by its crystallographic structure. Polycrystalline silicon degrades faster than single-crystalline

• ; pH 7.4 phosphate-buffered saline (PBS) at 37 °C) [37][53]. In contrast, Mo and W degrade significantly more slowly with dissolution rates of 0.001 µm/day (Mo; pH 7 buffer at rt) [58] and 0.48–1.44 µm/day (W; pH 7.4 SBF at rt) [59], which is advantageous for long-term device stability but poses

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

The role of biochar in combating microplastic pollution: a bibliometric analysis in environmental contexts

• Tuan Minh Truong Dang,
• Thao Thu Thi Huynh,
• Guo-Ping Chang-Chien and
• Ha Manh Bui

Beilstein J. Nanotechnol. 2025, 16, 1401–1416, doi:10.3762/bjnano.16.102

• ) MPs significantly reduce total exchangeable cations (Na+, K+, Mg2+, and Ca2+) by 4–6% [58]. Additionally, non-biodegradable MPs such as PE and PVC inhibit organic phosphate mineralization, alkaline phosphatase activity, and inorganic phosphate solubilization. By contrast, biodegradable MPs such as PLA

• do not affect alkaline phosphatase activity [59]. MPs also reduce available phosphorus (Olsen-P) levels by 9.7–38.6% (PE, PVC) and 38.4–73.6% polyactide (PLA) [60]. Furthermore, MPs compete with plant roots for phosphate adsorption, with distribution coefficients (Kd) in the order PVC (5.19 L/kg

Parylene-coated platinum nanowire electrodes for biomolecular sensing applications

• Chao Liu,
• Peker Milas,
• Michael G. Spencer and
• Birol Ozturk

Beilstein J. Nanotechnol. 2025, 16, 1392–1400, doi:10.3762/bjnano.16.101

• hydrochloride power (98%, Sigma-Aldrich) to 10 mL 0.01 M phosphate-buffered saline (pH 7.4, Sigma-Aldrich). This stock dopamine solution was added into 30 mL 0.01 M phosphate-buffered saline solution to get desired concentrations. Same Ag/AgCl reference and platinum counter electrodes as above were used in

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• in peroxisomes serve as substrates for lipid peroxidation. Clear-cell renal cell carcinoma (ccRCC) cells show elevated levels of PUFA-ePLs, likely due to their increased expression of alkylglycerone phosphate synthase (AGPS, an essential enzyme in the synthesis of PUFA-ePLs), which makes them

Enhancing the photoelectrochemical performance of BiOI-derived BiVO₄ films by controlled-intensity current electrodeposition

• Huu Phuc Dang,
• Khanh Quang Nguyen,
• Nguyen Thi Mai Tho and
• Tran Le

Beilstein J. Nanotechnol. 2025, 16, 1289–1301, doi:10.3762/bjnano.16.94

• converted into porous BiVO4 photoanodes. This approach yielded electrodes with enhanced PEC activity, achieving a photocurrent density of approximately 1.25 mA·cm−2 at 0.5 V vs RHE in neutral phosphate buffer under AM1.5G illumination. This study highlighted the potential of BiOI-derived BiVO4 as a template

Acrocomia aculeata oil-loaded nanoemulsion: development, anti-inflammatory properties, and cytotoxicity evaluation

• Verónica Bautista-Robles,
• Hady Keita,
• Edgar Julián Paredes Gamero,
• Layna Tayná Brito Leite,
• Jessica de Araújo Isaías Muller,
• Mônica Cristina Toffoli Kadri,
• Ariadna Lafourcade Prada and
• Jesús Rafael Rodríguez Amado

Beilstein J. Nanotechnol. 2025, 16, 1277–1288, doi:10.3762/bjnano.16.93

• to each well [41]. The plates were incubated for 1 h at 37 °C and were centrifuged at 3000 rpm at 5 °C for 15 min. After centrifugation, the concentration was suspended in 50 mL of phosphate-buffered saline (PBS, pH 7.4). The amount of hemoglobin was determined at 540 nm using a docetaxel (DTX) 880

Hydrogels and nanogels: effectiveness in dermal applications

• Jéssica da Cruz Ludwig,
• Diana Fortkamp Grigoletto,
• Daniele Fernanda Renzi,
• Wolf-Rainer Abraham,
• Daniel de Paula and
• Najeh Maissar Khalil

Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90

• physical properties [136]. They can be formed by preparing an aqueous solution of keratin at concentrations of 8 or 20%, for example [137]. With the "disulfide shuffling" method, phosphate-buffered saline (PBS) is used to dissolve 15% lyophilized keratin and 1.5% cysteine. This solution requires vigorous

Mechanical stability of individual bacterial cells under different osmotic pressure conditions: a nanoindentation study of Pseudomonas aeruginosa

• Lizeth García-Torres,
• Idania De Alba Montero,
• Eleazar Samuel Kolosovas-Machuca,
• Facundo Ruiz,
• Sumati Bhatia,
• Jose Luis Cuellar Camacho and
• Jaime Ruiz-García

Beilstein J. Nanotechnol. 2025, 16, 1171–1183, doi:10.3762/bjnano.16.86

• conditions. Of particular interest was the extraction and comparison of nanomechanical maps obtained in the low range of loading forces to quantify its morphology, membrane stiffness, Young’s modulus of elasticity and adhesion when PA was tested in hypotonic (Milli-Q water), isotonic (0.1 M phosphate

• –Hinton agar and again incubated for 24 h under the same conditions. Isolated colonies of PA were collected using a calibrated loop and suspended in 5 mL of phosphate buffer supplemented with 50 µL of Mueller–Hinton broth. The suspension was vortexed for 10 s before its preparation for AFM analysis

• then exposed to the desired osmolarity conditions for investigation. The applied solutions were ultrapure water (Milli-Q), phosphate-buffered saline solution (PBS), and 0.5 M sodium chloride. Bacteria were first analyzed in tapping mode to determine their location and observe their morphology

Fabrication of metal complex phthalocyanine and porphyrin nanoparticle aqueous colloids by pulsed laser fragmentation in liquid and their potential application to a photosensitizer for photodynamic therapy

• Taisei Himeda,
• Risako Kunitomi,
• Ryosuke Nabeya,
• Tamotsu Zako and
• Tsuyoshi Asahi

Beilstein J. Nanotechnol. 2025, 16, 1088–1096, doi:10.3762/bjnano.16.80

• = AlCl, Fe, Co, Zn) and Pt complex octaethylporphyrin (PtOEP) by nanosecond laser fragmentation of the corresponding microcrystalline powders in an aqueous solution of the amphiphilic polymer Pluronic® F-127. All nanoparticles dispersed stably in phosphate-buffered saline and cell culture media without

• polypropylene oxide (PPO) arranged in a triblock structure. Here, the stability in phosphate-buffered saline (PBS, pH 7.2), which is used widely in pharmacology and biomedical experiments, was examined. We evaluated the phototoxicity of the fabricated nanoparticle colloids in vitro against PC12 cells (a cell

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• nucleation of calcium and phosphate ions, imparting stiffness and resistance to bone. Additionally, collagen and noncollagenous matrix proteins contribute to bone formation by offering a scaffold for hydroxyapatite deposition [2][3]. Bone tissue consists of four main cell types: bone lining cells

• , while proteoglycans help form the organic matrix [8]. During matrix mineralization, calcium and phosphate ions generate hydroxyapatite crystals within the matrix region. Once bone formation is complete, some osteoblasts become embedded within the matrix, transforming into osteocytes, which leads to

• using an ultrasonic bath before treatment. Using an ultrasonic bath, US was applied to cells after NMs treatment and within the incubation period, 3 times for 5 s daily at 37 kHz for all in vitro experiments. After the incubation, the medium was removed and washed with phosphate buffered saline (1X PBS

Soft materials nanoarchitectonics: liquid crystals, polymers, gels, biomaterials, and others

• Katsuhiko Ariga

Beilstein J. Nanotechnol. 2025, 16, 1025–1067, doi:10.3762/bjnano.16.77

• , electronic devices, and electrochemical energy conversion and storage. Marmisollé and colleagues conducted research on “electrochemical nanoarchitectonics through polyaminobenzylamine–dodecyl phosphate complexes” and proposed a straightforward strategy for modifying electrode surfaces with self-assembled

• polyelectrolyte–surfactant complexes (Figure 10) [260]. The complex structures consist of polyaniline appended with amino groups and monododecyl phosphate. The intricate films were produced using a spin coating method. The films displayed distinct lamellar structures, resulting from the strong interactions

• between the phosphate groups of the complexes and the positively charged ammonium groups of the polyelectrolyte. The alignment of lamellae in a parallel orientation to the substrate is significantly influenced by the percentage of surfactant present. An increase in surfactant concentration results in the

Shape, membrane morphology, and morphodynamic response of metabolically active human mitochondria revealed by scanning ion conductance microscopy

• Eric Lieberwirth,
• Anja Schaeper,
• Regina Lange,
• Ingo Barke,
• Simone Baltrusch and
• Sylvia Speller

Beilstein J. Nanotechnol. 2025, 16, 951–967, doi:10.3762/bjnano.16.73

• the cytosolic abundant enzyme glyceraldehyde-3-phosphate dehydrogenase (Figure 2c). We analysed 21 metabolically active mitochondria from HeLa cells with SICM, resulting in a data set of 65 measurements (some mitochondria were measured several times). Shape analysis is based on the roundness of the

• demonstrate the quality of mitochondria of both isolation methods. (c) A minor signal for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) shows low contamination by cytosolic proteins. False-colour topographies and statistical analyses to illustrate the geometric properties of metabolically active

Serum heat inactivation diminishes ApoE-mediated uptake of D-Lin-MC3-DMA lipid nanoparticles

• Demian van Straten,
• Luuk van de Schepop,
• Rowan Frunt,
• Pieter Vader and
• Raymond M. Schiffelers

Beilstein J. Nanotechnol. 2025, 16, 740–748, doi:10.3762/bjnano.16.57

• by which FCS heat inactivation could affect cellular interactions with LNPs were studied in detail. Materials and Methods Buffers, reagents, materials Phosphate buffered saline (PBS), bovine serum albumin (BSA), thioflavin T (ThT), cholesterol and distearoylphosphatidylcholine (DSPC) were purchased

Efficiency of single-pulse laser fragmentation of organic nutraceutical dispersions in a circular jet flow-through reactor

• Tina Friedenauer,
• Maximilian Spellauge,
• Alexander Sommereyns,
• Verena Labenski,
• Tuba Esatbeyoglu,
• Christoph Rehbock,
• Heinz P. Huber and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2025, 16, 711–727, doi:10.3762/bjnano.16.55

• Materials Curcumin from turmeric rhizome (95%) was purchased from Thermo Fisher GmbH. Cannabidiol (≈99%) was provided by “Ott im Pott – CBD Shop Essen”. Acetonitrile, potassium dihydrogen phosphate, and orthophosphoric acid (>85%) for HPLC analysis were purchased in HPLC grade from VWR International GmbH

• −1 solution of potassium dihydrogen phosphate previously adjusted to pH 2.0 with phosphoric acid (A) and acetonitrile (B). The initial concentration of mobile phase B is increased from 30% to 70% within the time interval from 0 to 8 min, then further increased to 90%; this concentration is maintained

Colloidal few layered graphene–tannic acid preserves the biocompatibility of periodontal ligament cells

• Teissir Ben Ammar,
• Naji Kharouf,
• Dominique Vautier,
• Housseinou Ba,
• Nivedita Sudheer,
• Philippe Lavalle and
• Vincent Ball

Beilstein J. Nanotechnol. 2025, 16, 664–677, doi:10.3762/bjnano.16.51

• microhardness without compromising biocompatibility [7]. In another work, the formulation of an injectable calcium phosphate cement–chitosan–graphene oxide (GO) composite was found to be effective. This composite fostered the proliferation of human dental pulp stem cells [8]. Despite these promising findings

• Hoechst 33258 were obtained from MERCK Sigma-Aldrich Reagent (Darmstadt, Germany). α-MEM cell culture medium was purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Fetal bovine serum (FBS) and phosphate-buffered saline (PBS) were acquired from Dominique Dutscher (Brumath, France). The

Aprepitant-loaded solid lipid nanoparticles: a novel approach to enhance oral bioavailability

• Mazhar Hussain,
• Muhammad Farooq,
• Muhammad Asad Saeed,
• Muhammad Ijaz,
• Sherjeel Adnan,
• Zeeshan Masood,
• Muhammad Waqas,
• Wafa Ishaq and
• Nabeela Ameer

Beilstein J. Nanotechnol. 2025, 16, 652–663, doi:10.3762/bjnano.16.50

• microscopy (SEM), X-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA), in vitro drug release in 0.1 M HCl (pH 1.2) and phosphate-buffered saline (PBS, pH 7.4), and pharmacokinetic studies. The optimal formulation (APT-CD-NP4) containing the highest

• than the poloxamer 407-based formulations in phosphate-buffered saline (pH 7.4) and 0.1 N HCl (pH 1.2). The optimal SLN formulation APT-CD-NP4 is a promising tool for oral sustained-release dosage in order to improve the bioavailability of the BCS class-IV drug APT. Solubility analysis of APT and APT

• -loaded SLNs in 0.1 N HCl (pH 1.2) and phosphate-buffered saline (pH 7.4). (a) Zeta Potential and (b) particle size of APT-CD-NP4. SEM photomicrographs of (a, b) APT-CD-NP4 and (c, d) APT-PX-NP8. (a) FTIR spectra and (b) X-ray diffractograms of APT, poloxamer 407, β-CD, APT-CD-NP4, and APT-PX-NP8. (a) TGA

A formulation containing Cymbopogon flexuosus essential oil: improvement of biochemical parameters and oxidative stress in diabetic rats

• Ailton Santos Sena-Júnior,
• Cleverton Nascimento Santana Andrade,
• Pedro Henrique Macedo Moura,
• Jocsã Hémany Cândido dos Santos,
• Cauãn Torres Trancoso,
• Eloia Emanuelly Dias Silva,
• Deise Maria Rego Rodrigues Silva,
• Ênio Pereira Telles,
• Luiz André Santos Silva,
• Isabella Lima Dantas Teles,
• Sara Fernanda Mota de Almeida,
• Daniel Alves de Souza,
• Jileno Ferreira Santos,
• Felipe José Aidar Martins,
• Ana Mara de Oliveira e Silva,
• Sandra Lauton-Santos,
• Guilherme Rodolfo Souza de Araujo,
• Cristiane Bani Correa,
• Rogéria De Souza Nunes,
• Lysandro Pinto Borges and
• Ana Amélia Moreira Lira

Beilstein J. Nanotechnol. 2025, 16, 617–636, doi:10.3762/bjnano.16.48

• (surfactants + water, without EOCF), EOCF, and the selected microemulsion containing EOCF (M7-EOCF), at concentrations of 50, 100, and 200 µg/mL, and cultured under the previous conditions for further 24 h. At the end of this phase, the cell monolayer was washed twice with phosphate-buffered saline, and then