Nanoarchitectonics meets cell surface engineering: shape recognition of human cells by halloysite-doped silica cell imprints

• Elvira Rozhina,
• Ilnur Ishmukhametov,
• Svetlana Batasheva,
• Farida Akhatova and
• Rawil Fakhrullin

Beilstein J. Nanotechnol. 2019, 10, 1818–1825, doi:10.3762/bjnano.10.176

• equipped with a fluorite 100× objective and Dage xL (Dage-MTI) CCD camera. Images were obtained using Exponent 7 software (Dage-MTI). The dark-field images were overlapped with transmission fluorescence images using the image processing software GIMP. Cell proliferative activity was examined by flow

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• examination and the images were recorded with a Spot RT Slider camera (Diagnostic Instruments, Sterling Heights, MI, USA). The image processing was performed in Adobe Photoshop 6.0. Statistical analysis Statistical analysis was carried out using DellTM StatisticaTM 13.2 software (StatSoft, Tulsa, USA). The

Lipid nanostructures for antioxidant delivery: a comparative preformulation study

• Elisabetta Esposito,
• Maddalena Sguizzato,
• Markus Drechsler,
• Paolo Mariani,
• Federica Carducci,
• Claudio Nastruzzi,
• Giuseppe Valacchi and
• Rita Cortesi

Beilstein J. Nanotechnol. 2019, 10, 1789–1801, doi:10.3762/bjnano.10.174

• e/nm2 at 200 kV. The images were digitally recorded by a CCD camera (Ultrascan 1000, Gatan) using an image processing system (GMS 1.9 software, Gatan). In addition, the size distribution of the nanoparticles was performed by measuring 1000 nanoparticles for each cryo-TEM image by the digital

Ultraviolet patterns of flowers revealed in polymer replica – caused by surface architecture

• Anna J. Schulte,
• Matthias Mail,
• Lisa A. Hahn and
• Wilhelm Barthlott

Beilstein J. Nanotechnol. 2019, 10, 459–466, doi:10.3762/bjnano.10.45

• injection resin (RECKLI Injektionsharz EP, RECKLI GmbH, Herne, Germany). Scanning electron microscopy (SEM) Images were recorded using a Cambridge Stereoscan 200 SEM (Zeiss GmbH, Oberkochen, Germany). A digital image processing system (DISS 5, Version 5.4.17.0, Point Electronic GmbH, Halle, Germany) was

In situ characterization of nanoscale contaminations adsorbed in air using atomic force microscopy

• Jesús S. Lacasa,
• Lisa Almonte and
• Jaime Colchero

Beilstein J. Nanotechnol. 2018, 9, 2925–2935, doi:10.3762/bjnano.9.271

• kHz. Further details of how DAFM and Kelvin probe microscopy (KPM) [14] are implemented in our experiments is described elsewhere [42][50][51][52]. WSxM software was used for image processing [56]. Typically, a plane filter was applied to topography images; no filter is applied to the electrostatic

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• cells were treated with 2.5 to 50 µg mL−1 for 24 h under cell culture conditions. Viable cells were quantified by digital image processing (phase analysis). The data are expressed as mean ± SD (n = 5 independent experiments) given as the percentage of the control (cells cultured without nanoparticles

High-throughput micro-nanostructuring by microdroplet inkjet printing

• Hendrikje R. Neumann and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2018, 9, 2372–2380, doi:10.3762/bjnano.9.222

• .). Image processing was carried out with JPK SPM Data Processing. (A) Schematic representation of the operational principle of the Fuji Dimatix DMP-2800 printing head. 4 mL of gold-loaded micelle solution (BMCL) is stored in the cartridge storage unit that is connected to the cartridge head. Via the

• all nanoparticles, the particle analyzer was used (included in ImageJ) and the image was converted to a binary image. Finally, a freely accessible nearest-neighbor detection algorithm was employed for the determination of the nanoparticle distances [36]. Atomic force microscopy (AFM) imaging and image

• processing Atomic force microscopy (AFM) topographic imaging was employed to measure the roughness of the samples. Imaging was performed on a JPK NanoWizard 3 (JPK Instruments AG) operated in ac mode using ACTA cantilevers (spring constant ≈40 N/m, resonance frequency ≈300 kHz; Applied NanoStructuresInc

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

• Birgit Lengerer,
• Marie Bonneel,
• Mathilde Lefevre,
• Elise Hennebert,
• Philippe Leclère,
• Emmanuel Gosselin,
• Peter Ladurner and
• Patrick Flammang

Beilstein J. Nanotechnol. 2018, 9, 2071–2086, doi:10.3762/bjnano.9.196

• pixels/line using the Nanoscope software (Version 9.4). The scan rate was kept at 0.5 Hz. The Nanoscope Analysis image processing software (Version 1.90) was used for image analysis. The images were not filtered and only a 2nd order flattening procedure was applied to the raw data. Immunohistochemistry

Fabrication of photothermally active poly(vinyl alcohol) films with gold nanostars for antibacterial applications

• Mykola Borzenkov,
• Maria Moros,
• Claudia Tortiglione,
• Serena Bertoldi,
• Nicola Contessi,
• Silvia Faré,
• Angelo Taglietti,
• Agnese D’Agostino,
• Piersandro Pallavicini,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2018, 9, 2040–2048, doi:10.3762/bjnano.9.193

• , open source image processing software). By taking into account 20 consecutive planes, the total number of spots contained in a volume V = 25.8 × 25.8 × 1 μm × 20 = 13313 μm3 is obtained. A correction was applied since each spot can be counted for four consecutive planes due to the extension of the

Direct AFM-based nanoscale mapping and tomography of open-circuit voltages for photovoltaics

• Katherine Atamanuk,
• Justin Luria and
• Bryan D. Huey

Beilstein J. Nanotechnol. 2018, 9, 1802–1808, doi:10.3762/bjnano.9.171

• current (Figure 2a) and open-circuit voltage (Figure 2b) images. These signals are uniquely colored for simultaneous visualization when superimposed (Figure 2c), based on a simple 1:1 overlay of the two distinct color images using conventional image processing software (FIJI). Viewed in this manner, most

Evaluation of replicas manufactured in a 3D-printed nanoimprint unit

• Manuel Caño-García,
• Morten A. Geday,
• Manuel Gil-Valverde,
• Xabier Quintana and
• José M. Otón

Beilstein J. Nanotechnol. 2018, 9, 1573–1581, doi:10.3762/bjnano.9.149

• specific procedures for measuring these magnitudes. The underlying clue is to gather some simple numbers qualifying the samples. The original idea was to propose a single figure of merit, but this turned out unfeasible, since many parameters are derived from numerical data through image processing of an

• parameters proposed in this work, taken from AFM image processing, are a map of RMS differences from theoretical to actual heights, a histogram of oriented gradients (HOG), the average height, and the average height-to-pitch ratio. All these parameters are explained in the next sections. Numerical Parameters

• , as shown in Figure 2. Analogously to the pitch case, the mean master angle is considered the maximum achievable value. The lowest value is 0°. Image Processing Parameters The second script is written and executed on a MatLab interface employing the MatLab Computer Vision Toolbox. It utilizes the

Graphene composites with dental and biomedical applicability

• Sharali Malik,
• Felicite M. Ruddock,
• Adam H. Dowling,
• Kevin Byrne,
• Wolfgang Schmitt,
• Ivan Khalakhan,
• Yoshihiro Nemoto,
• Hongxuan Guo,
• Lok Kumar Shrestha,
• Katsuhiko Ariga and
• Jonathan P. Hill

Beilstein J. Nanotechnol. 2018, 9, 801–808, doi:10.3762/bjnano.9.73

• of 40 N/m. Image processing was carried out using the Nanoscope software. The X-ray photoelectron spectroscopy (XPS) measurements were performed on a Theta Probe spectrometer (Thermo Electron Co., Germany) using monochromatic Al Kα radiation (photon energy of 15 keV with maximum energy resolution of

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• tomography (CT) was performed at 130 kV that led to a slice thickness of 5 mm. Three-dimensional images were iteratively reconstructed based on four iterations and eight subsets (Flash 3D). Afterwards, the images were transferred into image processing software (Siemens Syngo.Via, Siemens Healthcare GmbH

Exploring wear at the nanoscale with circular mode atomic force microscopy

• Olivier Noel,
• Aleksandar Vencl and
• Pierre-Emmanuel Mazeran

Beilstein J. Nanotechnol. 2017, 8, 2662–2668, doi:10.3762/bjnano.8.266

• . Finally, we describe the advantages of this method and we report a relevant application example addressing a Cu/Al2O3 nanocomposite material used in industrial applications. Keywords: circular mode atomic force microscopy; composite materials; image processing; nanowear; wear mechanisms; Introduction

• track. The minimum and maximum wear volume values of these different results are determined in order to estimate the uncertainties. The image processing is fundamental for obtaining a clear description of the wear process. It is clearly illustrated in Figure 4 where the wear track on the image before

• the image processing (image B in Figure 4) is hardly distinguishable from the background. After having applied the image processing (image C), the circular wear track clearly contrasts with the background and details such as wear debris accumulation (not visible on the topographic image A before the

Comparing postdeposition reactions of electrons and radicals with Pt nanostructures created by focused electron beam induced deposition

• Julie A. Spencer,
• Michael Barclay,
• Miranda J. Gallagher,
• Robert Winkler,
• Ilyas Unlu,
• Yung-Chien Wu,
• Harald Plank,
• Lisa McElwee-White and
• D. Howard Fairbrother

Beilstein J. Nanotechnol. 2017, 8, 2410–2424, doi:10.3762/bjnano.8.240

• (L) = 10−6 Torr·s. Atomic force microscopy Deposits created by FEBID from cis-Pt(CO)2Cl2 were imaged before and after AH cleaning by atomic force microscopy (AFM) in noncontact mode with a 75 ± 15 kHz HQ:NCS18 probe (Mikromasch USA, Watsonville, CA) on a PicoSPM SE AFM. Image processing of line-by

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• nm laser lines. The analysis was performed using a 63×/NA 1.4 immersion oil lens. Image processing routines Analytical data were retrieved from the laser scanning micrographs (stacks of bright field and fluorescence channels) by means of dedicated ImageJ macro scripts (see Supporting Information File

Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications

• Arūnas Jagminas,
• Agnė Mikalauskaitė,
• Vitalijus Karabanovas and
• Jūrate Vaičiūnienė

Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174

• resolution. Image processing included flattening (2nd order) to remove the background slope caused by the irregularities of the piezoelectric scanner. The analysis was performed using the SpmLabAnalysis software (Veeco Instruments Inc., USA). Magnetization measurements were accomplished using a vibrating

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• . 12 micrographs were taken for each sample. The cell nuclei were automatically counted using open source ImageJ image processing software. Photoluminescence microphotographs of fixed cells co-cultivated with photoluminescent NDs (AR-40), 100 µg/mL (30 µg/cm2), were also taken using an IX71 microscope

Synthesis of [Fe(L_eq)(L_ax)]_n coordination polymer nanoparticles using blockcopolymer micelles

• Christoph Göbel,
• Ottokar Klimm,
• Florian Puchtler,
• Sabine Rosenfeldt,
• Stephan Förster and
• Birgit Weber

Beilstein J. Nanotechnol. 2017, 8, 1318–1327, doi:10.3762/bjnano.8.133

• with a MegaView III/iTEM image acquiring and processing system from Olympus Soft Imaging Systems (OSIS, Münster, Germany) and an Orius 830 SC200W/DigitalMicrograph system from Gatan (Munich, Germany). Particles size measurements were done with “ImageJ” image processing software by Wayne Rasband

Preparation of thick silica coatings on carbon fibers with fine-structured silica nanotubes induced by a self-assembly process

• Benjamin Baumgärtner,
• Hendrik Möller,
• Thomas Neumann and
• Dirk Volkmer

Beilstein J. Nanotechnol. 2017, 8, 1145–1155, doi:10.3762/bjnano.8.116

• equipped with a Gatan image filter. Holey carbon-coated copper grids were used for sample preparation. Atomic force topographic images were sampled by means of an Agilent 5500 AFM with MAC III controller operating in tapping mode. A polynomial background subtraction was applied for image processing

Bio-inspired micro-to-nanoporous polymers with tunable stiffness

• Julia Syurik,
• Ruth Schwaiger,
• Prerna Sudera,
• Stephan Weyand,
• Siegbert Johnsen,
• Gabriele Wiegand and
• Hendrik Hölscher

Beilstein J. Nanotechnol. 2017, 8, 906–914, doi:10.3762/bjnano.8.92

• . In order to account for this minimum pore size, Ramesh et al. [28] introduced a cut-off to a log-normal distribution of pore sizes, so the resulting distribution is cut around zero-values. As the pores were analysed based on automated image processing, we introduced a minimum detectable pore size of

Impact of air exposure and annealing on the chemical and electronic properties of the surface of SnO₂ nanolayers deposited by rheotaxial growth and vacuum oxidation

• Monika Kwoka and
• Maciej Krzywiecki

Beilstein J. Nanotechnol. 2017, 8, 514–521, doi:10.3762/bjnano.8.55

• using the PSIA XE-70 scanning microscope working in contact mode. Budget Sensors monolithic silicon probes ContAl-G (resonance frequency 13 kHz, force constant 0.2 N·m−1) were used. The XEI®, PSIA and Gwyddion® image processing software allowed us to correct sample inclination and distortions caused by

Structural and tribometric characterization of biomimetically inspired synthetic "insect adhesives"

• Matthias W. Speidel,
• Malte Kleemeier,
• Andreas Hartwig,
• Klaus Rischka,
• Angelika Ellermann,
• Rolf Daniels and
• Oliver Betz

Beilstein J. Nanotechnol. 2017, 8, 45–63, doi:10.3762/bjnano.8.6

Grazing-incidence optical magnetic recording with super-resolution

• Gunther Scheunert,
• Sidney. R. Cohen,
• René Kullock,
• Ryan McCarron,
• Katya Rechev,
• Ifat Kaplan-Ashiri,
• Ora Bitton,
• Paul Dawson,
• Bert Hecht and
• Dan Oron

Beilstein J. Nanotechnol. 2017, 8, 28–37, doi:10.3762/bjnano.8.4

• ). The laser focus, slightly different for each image, might also contribute to differences in the feature intensity, as well as standard AFM image processing. The scale bars in (a) and (b) are chosen to be 3 μm to show the correlation to the laser beam, as in Figure 3. Power absorption per volume within

Nanostructured SnO₂–ZnO composite gas sensors for selective detection of carbon monoxide

• Paul Chesler,
• Cristian Hornoiu,
• Susana Mihaiu,
• Cristina Vladut,
• Jose Maria Calderon Moreno,
• Mihai Anastasescu,
• Carmen Moldovan,
• Bogdan Firtat,
• Costin Brasoveanu,
• George Muscalu,
• Ion Stan and
• Mariuca Gartner

Beilstein J. Nanotechnol. 2016, 7, 2045–2056, doi:10.3762/bjnano.7.195

• were analyzed directly (samples were immobilized on a double-sided carbon tape, without coating). AFM measurements were carried in noncontact mode with an XE-100 apparatus from Park Systems (2011), using sharp tips (<8 nm tip radius; PPP-NCHR type from Nanosensors). The XEI (v.1.8.0) image processing