Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• , Figure S1A) and the mRNA level (Supporting Information File 1, Figure S1B). The expression of Dyn was analyzed only at the protein level. Our results demonstrated that A549 cells are proficient in both CME and CavME pathways. The effect of endocytic inhibitors on cell proliferation and morphology The

• experiments with the positive controls confirmed the ability of endocytic inhibitors to block the specific route of endocytosis. None of the endocytic inhibitors affected cell viability and proliferation activity with the exception of nocodazole (Noc) (Supporting Information File 1, Figure S2). Short-term

• exposure of cells to surface-modified MNPs and Noc affect substantially the cell proliferation and morphology. Noc affects microtubule formation, thus interfering with cytoskeleton structure and mitosis, leading to cell cycle arrest in G2/M [26]. As MNPs interfere with tubulin polymerization as well [27

Effect of different silica coatings on the toxicity of upconversion nanoparticles on RAW 264.7 macrophage cells

• Cynthia Kembuan,
• Helena Oliveira and
• Christina Graf

Beilstein J. Nanotechnol. 2021, 12, 35–48, doi:10.3762/bjnano.12.3

• nanoparticles in polymer shells decreases with incubation time due to the occurrence of proliferation and exocytosis [65]. Such effects can be related to the functionalization and size of the nanoparticles as well as to ion release, and modulate the toxicity of lanthanide-containing particles as a function of

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• extracellular glutamate concentrations in glioma cell lines in vitro was shown to be up to 500 µM, and glutamate stimulates glioma cell proliferation in vivo. Also, glial tumor cells ex vivo generate neurotoxic quantities of glutamate [3][4][5][6]. Excessive extracellular glutamate concentrations of 100 μM were

Photothermally active nanoparticles as a promising tool for eliminating bacteria and biofilms

• Mykola Borzenkov,
• Piersandro Pallavicini,
• Angelo Taglietti,
• Laura D’Alfonso,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2020, 11, 1134–1146, doi:10.3762/bjnano.11.98

• of 0.25 W/cm2 at 808 nm, lower than the ANSI limits) and the intrinsic nanochemical Ag NP bactericidal effect was observed. In order to combat bacterial adhesion and proliferation, recent techniques have been developed to functionalize photothermally active monolayers of gold nanostars on glass with

Uniform Fe₃O₄/Gd₂O₃-DHCA nanocubes for dual-mode magnetic resonance imaging

• Miao Qin,
• Yueyou Peng,
• Mengjie Xu,
• Hui Yan,
• Yizhu Cheng,
• Xiumei Zhang,
• Di Huang,
• Weiyi Chen and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2020, 11, 1000–1009, doi:10.3762/bjnano.11.84

• concentrations of FGDA nanocubes. At that stage, the L929 cells were probably still adapting to the media containing FGDA nanocubes and therefore were not proliferating. At later time points (24 and 48 h), the L929 cells treated with the FGDA nanocubes were proliferating normally and their proliferation rate was

• conclusion, FGDA nanocubes have no negative impact on cell viability or proliferation, which suggests that these nanocubes can be used for in vivo applications. In vivo MRI with FGDA nanocubes as a contrast agent To visualize the contrast and image quality provided by the FGDA nanocubes in vivo, T1-weighted

Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy

• Mengdan Chen,
• Jinshu Zeng,
• Weiwei Ruan,
• Zhenghong Zhang,
• Yuhua Wang,
• Shusen Xie,
• Zhengchao Wang and
• Hongqin Yang

Beilstein J. Nanotechnol. 2020, 11, 568–582, doi:10.3762/bjnano.11.45

• and invasion [59]. Kinsella’s group showed that the main problem in the treatment of cancer may be the invasive behavior of cancer cells [60]. The present investigations indicate that chemotherapy drugs could alter the mechanical properties of malignant tumors cells to attenuate cell proliferation

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• application in implants or tissue engineering. Another strategy for biomolecular functionalization is covalently linking the receptor specific ligands to one of the layer components that are known to interact with cancer cell receptors. For instance, the improved cell adhesion and proliferation was observed

Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes

• Alfredo Nuñez-Rivera,
• Pierrick G. J. Fournier,
• Danna L. Arellano,
• Ana G. Rodriguez-Hernandez,
• Rafael Vazquez-Duhalt and
• Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28

• (Figure 4C), corroborating the cargo release from BMV VLP inside tumor cells and gene silencing. BMV VLPs as siAkt1 nanocarriers The anti-cancer siRNA Akt1 (siAkt1) was also encapsidated in BVM-VLPs (Figure 4B). Akt1 is a kinase involved in the processes of cell proliferation, migration and transformation

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• that have been connected to the proliferation and invasion of lung cancer cells. These results indicate that the PVI complexes can be an effective agent to counter lung cancer. Keywords: anti-VEGF siRNA; gene silencing; lung cancer; microarray; poly(1-vinylimidazole); small interfering RNA (siRNA

• and blank polymer exhibited viabilities exceeding that of the untreated control cells while those cells treated with the polyplex exhibited a decreased viability. VEGF inhibition has been earlier reported to decrease the proliferation of cancer cells due to its ability to interfere with the MAPK and

• -regulated. The role of TFF1 in cancer remains controversial but many reports have demonstrated that TFF1 serves as a tumor suppressor gene that inhibits cancer cell proliferation and migration in epithelial cancers such as gastric, breast and pancreatic cancer [36]. Recent experimental evidence has revealed

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• plates and left to adhere overnight (5% v/v CO2 in air, 37 °C). Cells were then exposed to 0.25 mL of nanoparticle suspensions in full medium (concentration: 0.01–0.5 mg/mL) for 24 h, then determining viability using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS assay). Briefly

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

• Dávid Juriga,
• Evelin Sipos,
• Orsolya Hegedűs,
• Gábor Varga,
• Miklós Zrínyi,
• Krisztina S. Nagy and
• Angéla Jedlovszky-Hajdú

Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249

• established. Using metoprolol as a model drug, cell proliferation and drug release kinetics were studied at different LYS contents and in the presence of thiol groups. The optimal ratio of cross-linkers for the proliferation of periodontal ligament cells was found to be 60−80% LYS and 20−40% CYS. The

• is that it can partially decrease the anionic character of aspartic acid. In addition, LYS also facilitates the electrostatic interaction between anionic plasma membrane sites and cationic polymer sites. Therefore, it supports cell adhesion and proliferation [47]. The swelling, mechanical and

• proliferation reagent WST-1. The reagent was diluted (1:20) with αMEM containing no phenol red (Gibco, USA). Then, 200 μL solution was applied in each well. After incubation for 2 h at 37 °C, 150 μL of the supernatant solution was transferred from each well into an empty 96-well plate. The absorbance was

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• (Figure 1c). In contrast, exposure to cystabn, reduced cell proliferation over a 5 day period (Figure 1d), thus confirming that the addition of an N terminal cysteine did not interfere with GRPR binding. Exposure of NCI-H82 cells to the Tyr4-Bn agonist (Figure 1e) and cystabn antagonist peptide (Figure 1f

• ) caused no change in cell growth. These cell proliferation studies were performed in serum-free conditions in line with previous studies [24][25] for two principle reasons. Firstly, the removal of serum from the culture medium depletes bovine bombesin-like peptides that could otherwise stimulate cell

• GRPR depleted NCI-H82 cells. In contrast to results from NCI-H345 cells, the addition of Tyr4-Bn to NCI-H82 caused no noticeable increase in cell proliferation and cystabn effected no reduction in proliferation. This demonstrated that cystabn functionally targeted GRPR expressing cells in a specific

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

• Saban Kalay,
• Yurij Stetsyshyn,
• Volodymyr Donchak,
• Khrystyna Harhay,
• Ostap Lishchynskyi,
• Halyna Ohar,
• Yuriy Panchenko,
• Stanislav Voronov and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

• . DU145 cells internalize more P(AA-co-FA)-functionalized BNNTs because of their higher nutrition requirement for their fast proliferation and growth. While only P(AA-co-FA)-functionalized BNNT labeled cell nuclei were detectable in PNT1A control cells, the cells incubated with P(AA-co-FA)-functionalized

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• engineering [1][2] as they affect many cell functions such as cell migration [3][4], attachment, proliferation [5][6] and differentiation [7][8]. Substrate stiffness and topography are two of the most important ECM physical parameters in regulating cell functions [9]. A previous study shows that cells

Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

• Sabrina Barbosa de Souza Ferreira,
• Gustavo Braga,
• Évelin Lemos Oliveira,
• Jéssica Bassi da Silva,
• Hélen Cássia Rosseto,
• Lidiane Vizioli de Castro Hoshino,
• Mauro Luciano Baesso,
• Wilker Caetano,
• Craig Murdoch,
• Helen Elizabeth Colley and
• Marcos Luciano Bruschi

Beilstein J. Nanotechnol. 2019, 10, 2304–2328, doi:10.3762/bjnano.10.222

• drug has been evidenced and shown to act on a variety of molecular targets that regulate the proliferation and apoptosis, decrease the expression of NF-κB and increase insulin-like growth factor-binding protein 5 (IGFBP-5) and cytochrome P450, family 1, member A1 (CYP1A1) [32][33][34]. Moreover, CUR

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• sterol structure similarly to cholesterol, P2 phytosomes (P2Ps) were prepared to further improve the water solubility of P2. The P2Ps exhibited a particle size of 53.6 ± 0.3 nm with oval shape and a zeta potential of −4.0 ± 0.7 mV. P2Ps could inhibit the proliferation of lung cancer cells more

• or better antiproliferative effects after a 72 h incubation. The DiP and P2P were made up of drugs and phosphatidylcholine. The antiproliferative activities of DiP and P2P against A549 and PC9 cells originated from the drugs. Therefore, there is no huge difference in cell proliferation inhibition

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

• Xianping Liu,
• Chengjuan Du,
• Haichun Li,
• Ting Jiang,
• Zimiao Luo,
• Zhiqing Pang,
• Daoying Geng and
• Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

• Sigma (USA) and Cy7.5 NHS ester was purchased from Nanocs (USA). Fetal bovine serum (FBS), phosphate buffered saline (PBS), trypsin-EDTA (0.25%), high glucose Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin were purchased from Gibco (CA, USA). The MTT cell proliferation and

Nanoarchitectonics meets cell surface engineering: shape recognition of human cells by halloysite-doped silica cell imprints

• Elvira Rozhina,
• Ilnur Ishmukhametov,
• Svetlana Batasheva,
• Farida Akhatova and
• Rawil Fakhrullin

Beilstein J. Nanotechnol. 2019, 10, 1818–1825, doi:10.3762/bjnano.10.176

• occurs. However, flow cytometry-based cell proliferation monitoring performed with cells stained with 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE) dye has confirmed that cells coated with pure silica or silica/halloysite display a similar cell proliferation pattern as intact HeLa cells

• the inhibition of proliferation by the silica shells. On day 3, however, the fluorescence intensity in silica-coated cells was even higher than in the control cells, apparently due to the partial destruction of the shells and the release of HeLa cells. Although non-compromised viability is an

Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• –brain barrier using rat brain capillary endothelial cells (rBCEC4). All types of nanoparticles were taken up time-dependently by the rBCEC4 cells, albeit to a different extent, causing a time- and concentration-dependent decrease in cell viability. Nanoparticle exposure did not change cell proliferation

• cytotoxicity in HUVECs. Furthermore, Si-NPs were shown to induce oxidative stress and inflammation mediated by mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) [21] pathways that are related to cell proliferation and differentiation but also to

• , proliferation and inflammation in rBCEC4 cells Possible changes in protein expression representing inhibition or activation of several crucial proteins of different signaling pathways involved in regulatory processes including cell survival and proliferation were investigated with western blotting. The active

Biocompatible organic–inorganic hybrid materials based on nucleobases and titanium developed by molecular layer deposition

• Leva Momtazi,
• Henrik H. Sønsteby and
• Ola Nilsen

Beilstein J. Nanotechnol. 2019, 10, 399–411, doi:10.3762/bjnano.10.39

• tetra-isopropoxide (TTIP) with thymine, uracil, and adenine (Figure 1). We have recently reported a significant increase in the proliferation rate of rat conjunctival goblet cells cultured on substrates coated with these hybrid materials based on amino acids, and also the currently presented nucleobases

• , compared to uncoated glass coverslips using alamarBlue® proliferation assay [23]. The current contribution describes the growth of the films based on nucleobases in more detail. Results The growth dynamics of all three systems were investigated using in situ QCM, as shown in Figure 2 and Figure 3 and

• of the same samples was measured after each period of water treatment. The three hour and four day time periods are the durations in which cells were cultured on these substrates for cell attachment and cell proliferation assays, respectively, in our previous study [23]. Unlike for the Ti-amino acids

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• nanoparticles are size-dependent, it might be prudent to assume the same about their biotoxicity. Because of their size, nanoobjects are able to be internalized by living cells and affect basic cellular processes such as metabolism, proliferation, differentiation or lysis [10]. Hydroxyapatite (Hap, Ca10(PO4)6

• proliferation of macrophage cells J774.1 was noticed above 200 µg/mL for HApSA+Si and at 300 µg/mL for HApSA+Si and F201. In the case of CHO cells, HAp F202 had a toxic effect at concentrations of 200 and 300 µg/mL. The presence of CaHFAP300 also caused a toxic effect at a concentration of 300 µg/mL. Particle

• ][64][65]. Cell-growth inhibition could be related to the presence of a slight excess of calcium ions inside the cytoplasm, which disrupts the intracellular calcium homeostasis and inhibits cells transcriptional and translational processes [66][67], slowing down proliferation and cell growth. Calcium

Hybrid Au@alendronate nanoparticles as dual chemo-photothermal agent for combined cancer treatment

• Anouchka Plan Sangnier,
• Romain Aufaure,
• Laurence Motte,
• Claire Wilhelm,
• Erwann Guenin and
• Yoann Lalatonne

Beilstein J. Nanotechnol. 2018, 9, 2947–2952, doi:10.3762/bjnano.9.273

• under irradiation within the first biological window (650–900 nm). The Au@alendronate nanoplatform thus provided a combined antitumor activity through drug delivery and photothermal therapy. Au@alendronate NPs inhibited in vitro the proliferation of prostate cancer cells (PC3) in a dose-dependent manner

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• GmbH, Heidelberg, Germany). The cells were maintained at 37 °C in a humidified 5% CO2 atmosphere and sub-cultivated every 7–14 days, depending on the cell proliferation. Adherent cells were washed with phosphate-buffered saline solution (PBS, GIBCO, Invitrogen GmbH) and detached from the culture flasks

Size-selected Fe₃O₄–Au hybrid nanoparticles for improved magnetism-based theranostics

• Maria V. Efremova,
• Yulia A. Nalench,
• Eirini Myrovali,
• Anastasiia S. Garanina,
• Ivan S. Grebennikov,
• Polina K. Gifer,
• Maxim A. Abakumov,
• Marina Spasova,
• Makis Angelakeris,
• Alexander G. Savchenko,
• Michael Farle,
• Natalia L. Klyachko,
• Alexander G. Majouga and
• Ulf Wiedwald

Beilstein J. Nanotechnol. 2018, 9, 2684–2699, doi:10.3762/bjnano.9.251

• , and 20 μL of MTS reagent (CellTiter 96 aqueous non-radioactive cell proliferation assay, Promega, USA) was added to each well. Following 4 h of incubation at 37 °C in darkness, the wells were placed on a permanent magnet to remove the NPs from solution, and 100 μL of the obtained solution was