Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• delivery of doxorubicin into PC-3 prostate cells using a modified bombesin targeting peptide [30]. The authors showed a reduction in mouse PC-3 xenograft size compared to non-targeted doxorubicin liposomes and saline control, consistent with tumour accumulation of the delivery system. In summary, an

Frontiers in pharmaceutical nanotechnology

• Matthias G. Wacker

Beilstein J. Nanotechnol. 2019, 10, 2538–2540, doi:10.3762/bjnano.10.244

• active transport of nanoparticles into the central nervous system using the low-density lipoprotein receptor family [3][4][5][6], provided an entry route for the cytostatic drug doxorubicin into the brain. The drug delivery system has been tested in a phase II clinical trial and hopefully will make its

Incorporation of doxorubicin in different polymer nanoparticles and their anticancer activity

• Sebastian Pieper,
• Hannah Onafuye,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Martin Michaelis and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 2062–2072, doi:10.3762/bjnano.10.201

• . To investigate whether easy-to-prepare nanoparticles made of well-tolerated polymers may circumvent transporter-mediated drug efflux, we prepared poly(lactic-co-glycolic acid) (PLGA), polylactic acid (PLA), and PEGylated PLGA (PLGA-PEG) nanoparticles loaded with the ABCB1 substrate doxorubicin by

• solvent displacement and emulsion diffusion approaches and assessed their anticancer efficiency in neuroblastoma cells, including ABCB1-expressing cell lines, in comparison to doxorubicin solution. Results: The resulting nanoparticles covered a size range between 73 and 246 nm. PLGA-PEG nanoparticle

• preparation by solvent displacement led to the smallest nanoparticles. In PLGA nanoparticles, the drug load could be optimised using solvent displacement at pH 7 reaching 53 µg doxorubicin/mg nanoparticle. These PLGA nanoparticles displayed sustained doxorubicin release kinetics compared to the more burst

Doxorubicin-loaded human serum albumin nanoparticles overcome transporter-mediated drug resistance in drug-adapted cancer cells

• Hannah Onafuye,
• Sebastian Pieper,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Klaus Langer and
• Martin Michaelis

Beilstein J. Nanotechnol. 2019, 10, 1707–1715, doi:10.3762/bjnano.10.166

• . Here, we tested doxorubicin-loaded human serum albumin (HSA) nanoparticles in the neuroblastoma cell line UKF-NB-3 and its ABCB1-expressing sublines adapted to vincristine (UKF-NB-3rVCR1) and doxorubicin (UKF-NB-3rDOX20). Doxorubicin-loaded nanoparticles displayed increased anticancer activity in UKF

• -NB-3rVCR1 and UKF-NB-3rDOX20 cells relative to doxorubicin solution, but not in UKF-NB-3 cells. UKF-NB-3rVCR1 cells were re-sensitised by nanoparticle-encapsulated doxorubicin to the level of UKF-NB-3 cells. UKF-NB-3rDOX20 cells displayed a more pronounced resistance phenotype than UKF-NB-3rVCR1

• cells and were not re-sensitised by doxorubicin-loaded nanoparticles to the level of parental cells. ABCB1 inhibition using zosuquidar resulted in similar effects like nanoparticle incorporation, indicating that doxorubicin-loaded nanoparticles successfully circumvent ABCB1-mediated drug efflux. The

The systemic effect of PEG-nGO-induced oxidative stress in vivo in a rodent model

• Qura Tul Ain,
• Samina Hyder Haq,
• Abeer Alshammari,
• Moudhi Abdullah Al-Mutlaq and
• Muhammad Naeem Anjum

Beilstein J. Nanotechnol. 2019, 10, 901–911, doi:10.3762/bjnano.10.91

• has been utilized as a potent radiotracer and drug-delivery agent in vivo using positron emission tomography (PET) imaging by Jang and co-workers [31]. Liu et al. [32][33] used PEG for the first time to functionalize GO, which can then be use as a vehicle for anticancer drugs such as doxorubicin. The

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• anchored to different nanoparticles for enhancing their uptake into tumoral cells or for binding to tumour vessels. As an example, cyclic NGR, which binds to the aminopeptidase receptor (CD13), was grafted on the surface of temperature-sensitive liposomes loaded with doxorubicin (Dox) for the selective

• subcellular localizations [42][82]. There are many cytotoxic drugs, such as doxorubicin, that induce cell apoptosis through intercalation with nuclear DNA. Further, gene silencing therapies based on an effective delivery of short hairpin RNA (shRNA) bearing genes for small interfering RNA (siRNA) need nuclear

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• Experimental Medicine CAS, Vídeňská 1083, 142 20 Prague 4, Czech Republic 10.3762/bjnano.9.236 Abstract Doxorubicin-conjugated magnetic nanoparticles containing hydrolyzable hydrazone bonds were developed using a non-toxic poly[N-(2-hydroxypropyl)methacrylamide] (PHPMA) coating, which ensured good colloidal

• stability in aqueous media and limited internalization by the cells, however, enabled adhesion to the cell surface. While the neat PHPMA-coated particles proved to be non-toxic, doxorubicin-conjugated particles exhibited enhanced cytotoxicity in both drug-sensitive and drug-resistant tumor cells compared to

• free doxorubicin. The newly developed doxorubicin-conjugated PHPMA-coated magnetic particles seem to be a promising magnetically targeted vehicle for anticancer drug delivery. Keywords: cytotoxicity; doxorubicin; magnetic; nanoparticles; poly[N-(2-hydroxypropyl)methacrylamide]; Introduction Severe

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• both in vitro and in vivo studies [5]. Gold nanoparticles conjugated with a trans-activating transcriptional activator (TAT) peptide modification encapsulated with the drug doxorubicin showed enhanced toxicity in brain cancer models [6]. Further, mesoporous silica nanoparticles were successfully used

Nanoconjugates of a calixresorcinarene derivative with methoxy poly(ethylene glycol) fragments for drug encapsulation

• Alina M. Ermakova,
• Julia E. Morozova,
• Yana V. Shalaeva,
• Victor V. Syakaev,
• Aidar T. Gubaidullin,
• Alexandra D. Voloshina,
• Vladimir V. Zobov,
• Irek R. Nizameev,
• Olga B. Bazanova,
• Igor S. Antipin and
• Alexander I. Konovalov

Beilstein J. Nanotechnol. 2018, 9, 2057–2070, doi:10.3762/bjnano.9.195

• nanoassociates that are able to encapsulate organic substrates of different hydrophobicity, including drugs (doxorubicin, naproxen, ibuprofen, quercetin). The micelles of the macrocycle slowed down the release of the hydrophilic substrates in vitro. In physiological sodium chloride solution and phosphate

• -buffered saline, the micelles of the macrocycle acquire thermoresponsive properties and exhibit a temperature-controlled release of doxorubicin in vitro. The combination of the low toxicity and the encapsulation properties of the obtained calixresorcinarene–mPEG conjugate shows promising potential for the

• encapsulation of hydrophobic substrates The encapsulation of model compounds of different hydrophobicity by macrocycle 3 was studied (Table 2). These compounds were doxorubicin (Dox), an anthracycline antitumor antibiotic, quercetin (QC), a flavonol that exhibits decongestant, antispasmodic, antihistaminic

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• origin, such as adipogenic, osteogenic and chondrogenic cells [6]. MSC-based therapies are being increasingly investigated for their promising potential in cancer diagnostics and treatment [5][7][8]. In vivo studies have demonstrated that MSCs can effectively deliver nanorattle-encapsulated doxorubicin

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• , Erlangen, Germany) and analysed by means of suitable add-ons for planar scintigraphy (MM Oncology application) and SPECT-CT (mMR General application). Multilocal in-tissue penetration (ITP) depth analyses Multilocal in-tissue doxorubicin penetration depth analyses were performed after doxorubicin exposure

• (doxorubicin hydrochloride, Teva®, 2 mg/mL, Pharmachemie B.V., Haarlem, Netherlands) of two postmortem pig peritonea by HINAT-LAU and PIPAC-MIP. HINAT-LAU was performed with undiluted doxorubicin (drug chamber was filled with 5 mL) at a system pressure of 3 bar for 25 min that cumulated into an effective

• doxorubicin dose of 0.88 mL (i.e., 1.76 mg doxorubicin hydrochloride). PIPAC-MIP was performed with diluted doxorubicin (1 mL doxorubicin in 50 mL aqueous 0.9 wt % sodium chloride solution) at a volumetric flow rate of 30 mL/min for 100 s that led to an effective doxorubicin dose of 2.9 mL (i.e., 5.8 mg

Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications

• Arūnas Jagminas,
• Agnė Mikalauskaitė,
• Vitalijus Karabanovas and
• Jūrate Vaičiūnienė

Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174

• conjugated with targeting and chemotherapy agents, such as cancer stem cell-related antibodies and the anticancer drug doxorubicin, for early detection and improved treatment. In order to verify our findings, high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), FTIR

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• molecules with drugs is currently considered a perspective biomedical strategy. The formation of a stable non-covalent nanocomplex of С60 fullerene with doxorubicin (C60+Dox) in aqueous solution was confirmed theoretically and experimentally [23][34][36]. The antitumor action of the C60+Dox nanocomplex was

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• studied for targeting and delivery of chemotherapeutic drugs for glioma treatment. Fang et al. prepared core–shell nanocapsules for co-delivery of the hydrophilic drug doxorubicin, and the hydrophobic drug curcumin. Their results showed that the synergistic cytotoxic effect on RG2 glioma cells was

Dispersion of single-wall carbon nanotubes with supramolecular Congo red – properties of the complexes and mechanism of the interaction

• Anna Jagusiak,
• Barbara Piekarska,
• Tomasz Pańczyk,
• Małgorzata Jemioła-Rzemińska,
• Elżbieta Bielańska,
• Barbara Stopa,
• Grzegorz Zemanek,
• Janina Rybarska,
• Irena Roterman and
• Leszek Konieczny

Beilstein J. Nanotechnol. 2017, 8, 636–648, doi:10.3762/bjnano.8.68

• , antigen-bound antibodies, cell-surface receptors) [29][34][35]. Congo red supramolecular assemblies can incorporate (through intercalation) other molecules – especially those containing planar, aromatic rings – e.g., doxorubicin, rhodamine B, other bis-azo dyes [36][37][38]. Thus CR could play a role of a

Comparison of the interactions of daunorubicin in a free form and attached to single-walled carbon nanotubes with model lipid membranes

• Dorota Matyszewska

Beilstein J. Nanotechnol. 2016, 7, 524–532, doi:10.3762/bjnano.7.46

• mechanisms proposed to explain the cellular uptake of CNTs including the passive diffusion in a non-invasive manner (tiny nanoneedle mechanism) [18]. Carbon nanotubes have been successfully used to transport different types of anticancer agents including camptothecin, doxorubicin and daunorubicin [19]. The

Using natural language processing techniques to inform research on nanotechnology

• Nastassja A. Lewinski and
• Bridget T. McInnes

Beilstein J. Nanotechnol. 2015, 6, 1439–1449, doi:10.3762/bjnano.6.149

• trials investigating nano versus non-nano drugs. These include facilitating comparisons between clinical trials testing nano and non-nano drug formulations involving the same active ingredient (e.g., Doxil = pegylated liposome [nano] encapsulated doxorubicin compared to Adriamycin = doxorubicin). In

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• doxorubicin-loaded silica-encapsulated manganese oxide NPs. The TEM images showed that elemental Mn was equally distributed in the whole silica matrix. The in vitro and in vivo studies suggested that these nanocomposites can be used as a vehicle for pH-responsive intracellular release of doxorubicin. 2.5 Iron

Synthesis of boron nitride nanotubes and their applications

• Saban Kalay,
• Zehra Yilmaz,
• Ozlem Sen,
• Melis Emanet,
• Emine Kazanc and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2015, 6, 84–102, doi:10.3762/bjnano.6.9

• in the presence and absence of a magnetic field and higher cell-associated uptake was found in the presence of a magnetic field. Then, the composites were loaded with doxorubicin (dox) to investigate the viability of LNCaP prostate cancer cells in the magnetic field. It was found that dox-loaded BNNT

• –NaGdF4:Eu composites had higher toxicity in the presence of a magnetic field due to increased cellular uptake of the composites and thus increased doxorubicin delivery. It can be said that the BNNT–NaGdF4:Eu composites increase the chemotherapy efficiency by the use of an external magnetic field [84

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• efficacy of cancer treatments as stand-alone therapies and in combination with standard chemotherapeutic treatment regimens. The current standard of care for metastatic cancer, doxorubicin (DOX), is presented with challenges, namely toxicity due to a lack of specificity and targeted delivery. Nano-enabled

• ; doxorubicin; nanodiamond; prostate cancer; targeted drug delivery; Introduction Prostate cancer is the most frequently diagnosed malignancy in men [1]. Typically the disease is slow growing, but in some cases it progresses to an aggressively metastatic state. When prostate cancer becomes metastatic, the

• current standard of care is chemotherapy, which involves the use of toxic anticancer drugs, like doxorubicin (DOX), to treat cancers by inducing apoptosis. DOX has had high success rates with treating prostate cancer [2]. However, it can cause major side effects such as hair loss, nausea [2][3], and

In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

• Tianxun Gong,
• Douglas Goh,
• Malini Olivo and
• Ken-Tye Yong

Beilstein J. Nanotechnol. 2014, 5, 546–553, doi:10.3762/bjnano.5.64

• or antigens to be linked to their surface for targeted delivery and imaging [16][17]. More importantly, one is not able to use CTAB-coated AuNRs as a carrier for drug delivery of water insoluble anti-cancer agents (e.g., doxorubicin, paclitaxel) to the cancerous area since their surface is