Ultrathin water layers on mannosylated gold nanoparticles

• Maiara A. Iriarte Alonso,
• Jorge H. Melillo,
• Silvina Cerveny,
• Yujin Tong and
• Alexander M. Bittner

Beilstein J. Nanotechnol. 2025, 16, 2183–2198, doi:10.3762/bjnano.16.151

• good contrast [25]. The STEM images in dark-field mode (imaging scattered electrons) show the gold cores as bright structures, whereas the thin organic layers are darker. The carbon grid yields practically no scattering and appears essentially black, such that both the core and shell of the particles

• sensing, imaging, or drug delivery systems. The success of these platforms stems from their dispersion in water, stability, and biocompatibility in fully hydrated states, as well as in biological fluids. Our investigation shows a novel approach to these particles by testing the hydration properties under

• for ppp polarization (all beams polarized normal to the surface) and 2 min for ssp (laser beams polarized parallel to the surface, sum frequency emission and up-conversion polarized perpendicular and infrared parallel to the plane of incidence). Electron microscopy imaging SEM and STEM images were

Microplastic pollution in Himalayan lakes: assessment, risks, and sustainable remediation strategies

• Sameeksha Rawat,
• S. M. Tauseef and
• Madhuben Sharma

Beilstein J. Nanotechnol. 2025, 16, 2144–2167, doi:10.3762/bjnano.16.148

• than SEM. It is particularly useful in morphological examination, size grading, and rapid particle identification. The combination of optical microscopes and modern digital imaging techniques allows for automatic particle classification and counting, which greatly decreases the extent of human

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• , these materials have also been used in various imaging applications for the detection of cancer cells. Ag2Te and Ag2S nanocrystals (NCs) were used in cancer imaging studies by Nieves and colleagues. In this study, computed tomography contrasts changes of NCs injected into mice were examined at 2 and 24

• findings were consistent across various imaging modalities. Analyzing the HRTEM image in Figure 2c revealed a lattice d-spacing of 3.214 Å, corresponding precisely to the (111) crystallographic planes. The SAED patterns in Figure 2d exhibited distinct, discontinuous diffraction rings with sharp points

Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation

• Michał Bartkowski,
• Francesco Calzaferri and
• Silvia Giordani

Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144

• energy sectors (Figure 1). NMs have seen use as antimicrobial agents [1], catalysts [2], bioimaging agents [3][4][5][6], magnetic particle imaging agents [7], nanofluids [8], antiviral agents [9], photothermal convertors [10], and in environmental remediation [11]. Topically, the biomedical applications

Multifrequency AFM integrating PeakForce tapping and higher eigenmodes for heterogeneous surface characterization

• Yanping Wei,
• Jiafeng Shen,
• Yirong Yao,
• Xuke Li,
• Ming Li and
• Peiling Ke

Beilstein J. Nanotechnol. 2025, 16, 2077–2085, doi:10.3762/bjnano.16.142

• ) technique that synergistically integrates PeakForce tapping mode with higher eigenmode vibrations to achieve simultaneous high-resolution topographical imaging and to access additional contrast channels for distinguishing material regions or compositions. Unlike conventional multimodal AFM, our method

• signals can be correlated with specific interaction types, such as conservative and dissipative processes [10][11][12]. Despite these advances, critical limitations persist. The inherent nonlinearity of tip–sample dynamics in tapping and multimodal AFM often introduces imaging artifacts. Typical examples

• traditional dynamic modes, PFT eliminates the need for intricate cantilever tuning by real-time regulation of the interaction force at each scan point. This innovation not only simplifies operation but also enables high-resolution topographic imaging with exceptional stability, even in challenging

Stereodiscrimination of guests in chiral organosilica aerogels studied by ESR spectroscopy

• Sebastian Polarz,
• Yasar Krysiak,
• Martin Wessig and
• Florian Kuhlmann

Beilstein J. Nanotechnol. 2025, 16, 2034–2054, doi:10.3762/bjnano.16.140

• measurements more difficult. There are other valuable methods for investigating transport in porous media such as gas-adsorption methods, X-ray tomography [24][25], neutron imaging techniques [26][27], optical imaging techniques [27][28], or impedance spectroscopy [29][30]. For all of them, it is difficult to

• ), quantitative evaluation of the spectra allowed for investigating various confinement effects. Our focus was on understanding how a particular functionalization of the surfaces in combination with 3CP and the solvent influences transport properties, and novel ESR imaging techniques were used to determine

Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• -sectional imaging, and attenuated total reflectance with Fourier transform infrared (ATR-FTIR) spectroscopy provided compelling evidence for the successful formation of the intended core–shell structure. The resulting nanofibers exhibited surface hydrophobicity, suggesting potential for anti-adhesive

• SEM imaging of the cross-section of the HA+NE2/PLA sample was performed before and after core removal to observe the core–shell structure. For core removal, a portion of the sample was washed multiple times with distilled water, followed by ethanol, and then dried at room temperature. Samples, both

• distribution of the core within nanofibers produced via coaxial electrospinning. A disk scanning unit confocal microscope (Olympus, Japan) was utilized for this analysis. Fluorescein was added to the core solution (1% HA + 2% NE) to enable fluorescence imaging during microscopic observation. Thermogravimetric

The cement of the tube-dwelling polychaete Sabellaria alveolata: a complex composite adhesive material

• Emilie Duthoo,
• Aurélie Lambert,
• Pierre Becker,
• Carla Pugliese,
• Jean-Marc Baele,
• Arnaud Delfairière,
• Matthew J. Harrington and
• Patrick Flammang

Beilstein J. Nanotechnol. 2025, 16, 1998–2014, doi:10.3762/bjnano.16.138

• electron imaging, the anterior parts of a few worms as well as some reconstructed tube fragments were fixed in Bouin’s fluid for 24 h, dehydrated in graded ethanol, dried by the critical-point method, and mounted on aluminium stubs using carbon adhesive tabs. The samples were then coated with gold

• unfixed, air-dried tube fragment that was subsequently broken, SEM imaging and microanalysis of a fractured cement spot showed one NaCl crystal within each of the pores (Figure 2E, Supporting Information File 1, Figure S1). A decalcified cement spot that held two glass beads together was also observed in

Laser ablation in liquids for shape-tailored synthesis of nanomaterials: status and challenges

• Natalie Tarasenka

Beilstein J. Nanotechnol. 2025, 16, 1963–1997, doi:10.3762/bjnano.16.137

• , Figure 2 demonstrates the correlation of the shape of laser-induced plasma generated in the electric field applied directly to a Zn target with NP structure. The plasma imaging (Figure 2b,c) demonstrates clear differences in shape depending on the applied field direction. In case of cathode ablation, the

Mechanical property measurements enabled by short-term Fourier-transform of atomic force microscopy thermal deflection analysis

• Thomas Mathias,
• Roland Bennewitz and
• Philip Egberts

Beilstein J. Nanotechnol. 2025, 16, 1952–1962, doi:10.3762/bjnano.16.136

• ); mechanical property measurements; surface science; Introduction Atomic force microscopy (AFM) has become an indispensable tool for imaging the surface topography on a variety of surfaces [1]. Since the invention of the AFM [2], several other modes of AFM have been developed, including friction force

Evaluating metal-organic precursors for focused ion beam-induced deposition through solid-layer decomposition analysis

• Benedykt R. Jany,
• Katarzyna Madajska,
• Aleksandra Butrymowicz-Kubiak,
• Franciszek Krok and
• Iwona B. Szymańska

Beilstein J. Nanotechnol. 2025, 16, 1942–1951, doi:10.3762/bjnano.16.135

• the thickness of the precursor layer on the substrate. (2) Performing SEM imaging of the growth layers, which provide detailed information about the surface structure and composition of the precursor layer. They are essential for understanding how the precursors are decomposed under the following FIB

• island-like features become more apparent. The precursor layer underwent decomposition leading to the development of surface features enriched with metallic element of the primary film. The quantitative changes are presented in Figure 2b, which shows the mean BSE signal intensity, acquired while imaging

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• , respectively, 18.9-fold and 17.3-fold higher luciferase expression compared to 5 mol % PEG LNPs. Fundus imaging and immunohistochemistry confirmed robust tdTomato fluorescence in the retinal pigment epithelium (RPE), with a twofold to 3.4-fold increase in signal, showing the 0.5% PEG LNPs consistently

• . Imaging and histological analysis showed that maleimide-LNPs remained localized near the injection site, implying enhanced interaction with the tumor microenvironment. In contrast, carboxylic acid-LNPs showed a reduced cellular association, likely due to electrostatic repulsion [52]. PDP-LNPs had minimal

Low-temperature AFM with a microwave cavity optomechanical transducer

• Ermes Scarano,
• Elisabet K. Arvidsson,
• August K. Roos,
• Erik Holmgren,
• Riccardo Borgani,
• Mats O. Tholén and
• David B. Haviland

Beilstein J. Nanotechnol. 2025, 16, 1873–1882, doi:10.3762/bjnano.16.130

• /bjnano.16.130 Abstract We demonstrate atomic force microscopy (AFM) imaging with a microcantilever force transducer where an integrated superconducting microwave resonant circuit detects cantilever deflection using the principles of cavity optomechanics. We discuss the detector responsivity and added

• refrigerator (DR). We operate the microscope with surface-tracking feedback using the two most common imaging modes of dynamic AFM: amplitude modulation (AM-AFM) and frequency modulation (FM-AFM) [19]. One of the biggest challenges of operating an AFM in a closed-cycle DR is the pulse tube cryogenic head

• can therefore be made light and compact, significantly reducing their susceptibility to external vibrations, the complexity of vibration isolation, and their thermal mass. For this prototype, we implement a rather simple vibration isolation which, as we will show, is sufficient to demonstrate imaging

Self-assembly and adhesive properties of Pollicipes pollicipes barnacle cement protein cp19k: influence of pH and ionic strength

• Shrutika Sawant,
• Anne Marie Power and
• J. Gerard Wall

Beilstein J. Nanotechnol. 2025, 16, 1863–1872, doi:10.3762/bjnano.16.129

• study, we investigated the influence of environmental parameters on the self-assembly of recombinant cp19k, a key adhesive protein in Pollicipes pollicipes. Using TEM imaging, a low pH (4.0) and high salt concentration (600 mM NaCl) environment, mimicking P. pollicipes gland conditions, was identified

• washes in dH2O. Negative staining with uranyl acetate was performed by incubating 5 µL of R1000 UA-Zero EM stain (Agar Scientific) on grids for 3 min. Grids were washed five times with dH2O and air-dried overnight. Bright-field TEM imaging was carried out using a Hitachi H7000 microscope operated at an

• ) rPolcp19k-his incubated at pH 4.0, 150 mM NaCl for 21 days, on hydrophilic surface, at 11.5× magnification. Supporting Information Supporting Information File 22: Additional figures. Acknowledgements The authors acknowledge the facilities of the Anatomy Imaging and Microscopy Facility at the University of

Phytol-loaded soybean oil nanoemulsion as a promising alternative against Leishmania amazonensis

• Victória Louise Pinto Freire,
• Mariana Farias Alves-Silva,
• Johny W. de Freitas Oliveira,
• Matheus de Freitas Fernandes-Pedrosa,
• Alianda Maira Cornélio,
• Marcelo de Souza-Silva,
• Thayse Silva Medeiros and
• Arnóbio Antônio da Silva Junior

Beilstein J. Nanotechnol. 2025, 16, 1826–1836, doi:10.3762/bjnano.16.126

• Company, Hillsboro, OR, USA). The samples were diluted in purified water at a 1:20 (v/v) ratio, and a drop of the diluted suspension was deposited onto square-mesh copper grids and allowed to adsorb for 2 min. The grids were then air-dried at room temperature prior to imaging. Cytocompatibility Mammalian

Exploring the potential of polymers: advancements in oral nanocarrier technology

• Rousilândia de Araujo Silva,
• Igor Eduardo Silva Arruda,
• Luise Lopes Chaves,
• Mônica Felts de La Roca Soares and
• Jose Lamartine Soares Sobrinho

Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122

Advances of aptamers in esophageal cancer diagnosis, treatment and drug delivery

• Yang Fei,
• Hui Xu,
• Chunwei Zhang,
• Jingjing Wang and
• Yong Jin

Beilstein J. Nanotechnol. 2025, 16, 1734–1750, doi:10.3762/bjnano.16.121

• obtain nucleic acid aptamer S3-2-3 with binding specificity to ESCC cells. After labeled with Cy5 dye, it can yield highly specific fluorescence imaging for ESCC tissues, providing accurate display tools for clinical diagnosis. The remarkably short 18-nucleotide length of aptamer S3-2-3 enables its

• aptamers. After Cy3 staining, the results showed that the two staining results were similar. The imaging method of the aptamer is simpler, indicating that the aptamer SYL3C can be used as a molecular diagnostic tool instead of antibody. Although there are many potential biomarkers for ESCC, and noninvasive

• single-stranded DNA aptamer Te4, which specifically binds to TE-1 cells, through Cell-SELEX and further formed aptamer–DOX complexes to deliver DOX directly to cancer cells. In vivo imaging showed that the fluorescence signal of the complex weakened after 150 min, and the circulation time was short

Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• was analyzed using transmission electron microscopy (TEM) with a MORGAGNI 268D (FEI Company, USA), operated at 80 kV. Before imaging, the samples were sonicated in an ultrasound bath for 15 min, and a drop of the suspension was placed onto a copper grid (200 mesh) coated with formvar/carbon. Excess

Beyond the bilayer: multilayered hygroscopic actuation in pine cone scales

• Kim Ulrich,
• Max David Mylo,
• Tom Masselter,
• Fabian Scheckenbach,
• Sophia Fischerbauer,
• Martin Nopens,
• Silja Flenner,
• Imke Greving,
• Linnea Hesse and
• Thomas Speck

Beilstein J. Nanotechnol. 2025, 16, 1695–1710, doi:10.3762/bjnano.16.119

• dependent hygroscopic tissue shrinkage The general imaging and analysis protocol followed the procedure presented in Ulrich and colleagues [29]. A pine cone scale was isolated from the second of the collected cones and transported to Hamburg, Germany, where the scale was soaked in water overnight to improve

• imaging beamline P05 operated by Helmholtz-Zentrum Hereon at PETRA III (DESY Deutsches Elektronen Synchrotron, Hamburg, Germany). Phase contrast-based near-field holotomography was used to image our samples, utilizing a 300 µm gold Fresnel zone plate to focus the monochromatic beam with an energy of 11

• measurements should include more cells to avoid conflicting results for the radial shrinkage. In addition, the original orientation during specimen preparation and imaging should remain traceable in order to differentiate between ab-/adaxial and lateral directions in the resulting images. The tissue average

Cross-reactivities in conjugation reactions involving iron oxide nanoparticles

• Shoronia N. Cross,
• Katalin V. Korpany,
• Hanine Zakaria and
• Amy Szuchmacher Blum

Beilstein J. Nanotechnol. 2025, 16, 1504–1521, doi:10.3762/bjnano.16.106

• [51][52]. In the context of magnetic resonance imaging, small molecule ligands also offer advantages over thicker, polymeric coatings as superior contrast agents [53][54][55]. To investigate amine cross-reactivities, we use propargylamine (PPA, Figure 1c) and a series of primary amine-containing dyes

Nanomaterials for biomedical applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Filippo Pierini,
• Seda Kizilel and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 1499–1503, doi:10.3762/bjnano.16.105

• this progress. The use of nanomaterials has notably contributed to advancements in the fields of disease diagnosis, treatment, and prevention. They can closely interact with cells and molecules of the body due to their small size, which helps to diagnose, enhance imaging, and repair damaged tissues

• adverse effects and increasing the success rate of the delivery. Since nanomaterials can be tunable, the vast majority of health sectors are investigating their potential in a wide range of applications, such as targeted drug delivery, gene therapy, tissue regeneration, imaging, and diagnostic tools [2

• diagnostics and medical imaging. Using these materials, physicians diagnose diseases earlier and more accurately than before [4]. In tissue engineering, nanofibers are being used to develop scaffolds to promote the proliferation of cells. These scaffolds aid patients suffering from chronic wounds as they

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

• mechanism of NPs in PLAL has been investigated extensively, primarily through the use of laser-induced fluorescence analysis and shadowgraph analysis [17][18][19][20][21]. Use of small-angle X-ray scattering, wide-angle X-ray scattering, and X-ray imaging techniques has enabled a more comprehensive

Photochemical synthesis of silver nanoprisms via green LED irradiation and evaluation of SERS activity

• Tuan Anh Mai-Ngoc,
• Nhi Kieu Vo,
• Cong Danh Nguyen,
• Thi Kim Xuan Nguyen and
• Thanh Sinh Do

Beilstein J. Nanotechnol. 2025, 16, 1417–1427, doi:10.3762/bjnano.16.103

• (TEM, JEM-1400, Japan). For TEM analysis, a droplet of the nanoparticle dispersion was deposited onto a 3 mm copper grid and allowed to dry at room temperature. For FESEM analysis, the dried sample was mounted on conductive carbon tape and imaged at an accelerating voltage of 10 kV. TEM imaging was

Parylene-coated platinum nanowire electrodes for biomolecular sensing applications

• Chao Liu,
• Peker Milas,
• Michael G. Spencer and
• Birol Ozturk

Beilstein J. Nanotechnol. 2025, 16, 1392–1400, doi:10.3762/bjnano.16.101

• complete as intended and these electrodes were discarded. A thin layer of copper deposition on the exposed platinum nanowire tip was further examined using SEM imaging and energy-dispersive X-ray spectroscopy (EDS) elemental composition analysis. Figure 2b presents an SEM image of the copper-coated

Automated collection and categorisation of STM images and STS spectra with and without machine learning

• Dylan Stewart Barker and
• Adam Sweetman

Beilstein J. Nanotechnol. 2025, 16, 1367–1379, doi:10.3762/bjnano.16.99

• Dylan Stewart Barker Adam Sweetman School of Physics and Astronomy, University of Leeds, Leeds LS2 9JT, United Kingdom 10.3762/bjnano.16.99 Abstract Atomic resolution scanning probe microscopy, and in particular scanning tunnelling microscopy (STM) allows for high-spatial-resolution imaging and

• accomplished via assessment of the imaging quality on the target molecule and also the characteristics of the scanning tunnelling spectra (STS) on clean metal surfaces. Critically for spectroscopic experiments, assessment of the spatial resolution of the image is not sufficient to ensure a high-quality tip for

• spectroscopic measurements. The ability to automate this process is a key aim in development of high resolution scanning probe materials characterisation. In this paper, we assess the feasibility of automating the assessment of imaging quality, and spectroscopic tip quality, via both machine learning (ML) and