Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model

• Jennifer Y. Kasper,
• Lisa Feiden,
• Maria I. Hermanns,
• Christoph Bantz,
• Michael Maskos,
• Ronald E. Unger and
• C. James Kirkpatrick

Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54

• (3.7%) in CS buffer (PIPES 0.1 M, EGTA 1 mM, 4% polyethylene glycol 800, NaOH 0.1 M) for 20 min at room temperature, and washed three times with PBS. Cell membranes were then permeabilized with 0.2% Triton X-100 in PBS for 10 min. After washing three times in PBS cells were stained with primary

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• cetyltrimetylammonium bromide (CTAB) in water. Furthermore, we investigated the effects of GNP that were linked to thiol-terminated polyethylene glycol (SH-PEG, MW of PEG: 5 kDa) with different reactive groups: cationic (-NH2), anionic (-COOH) or neutral (-OCH3). 2) Asymmetric Au@metal oxide nanoparticles have an

Mechanical properties of MDCK II cells exposed to gold nanorods

• Anna Pietuch,
• Bastian Rouven Brückner,
• David Schneider,
• Marco Tarantola,
• Christina Rosman,
• Carsten Sönnichsen and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

• either cetyl-trimethylammonium bromide (CTAB) or biocompatible polyethylene glycol (PEG) coated gold nanoparticles in different concentrations. We also examined structural rearrangement of the cytoskeleton via fluorescence microscopy and by that tried to gain a deeper understanding of how gold

• of gold nanorods were obtained from UV–vis spectroscopy using their optical extinction value at 400 nm and a molar extinction coefficient of 1.1 × 109 Lmol−1cm−1 assuming the aforementioned particle size found by TEM. In order to replace the CTAB, functionalized polyethylene glycol thiols (X-PEG-SH

Electrostatic interplay: The interaction triangle of polyamines, silicic acid, and phosphate studied through turbidity measurements, silicomolybdic acid test, and ²⁹Si NMR spectroscopy

• Anne Jantschke,
• Katrin Spinde and
• Eike Brunner

Beilstein J. Nanotechnol. 2014, 5, 2026–2035, doi:10.3762/bjnano.5.211

• negatively charged silica particles [18][20]. Subsequent in vitro studies support the idea of polyamine-stabilized sols [21][22]. In contrast to the charged PAH, uncharged polymers such as polyvinylpyrrolidone or polyethylene glycol cannot undergo such a self-assembly process driven by electrostatic

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• localization of silver in the perinuclear region was observed (Figure 5A). In none of the investigated cells an indication for uptake into the nucleus was found, which is in agreement with a quantitative TEM analysis of citrate- or polyethylene glycol (PEG)-stabilized gold nanoparticles, in which no particles

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• [91] and drug delivery to substrates for biomolecular imaging [92][93], and from tissue scaffolds [94] to electrodes for neural stimulation [95]. For instance, Yang and colleagues [96] investigated the possibility of using polyethylene glycol (PEG)-coated nanographene sheets showing absorption in the

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• groups, but also with polyethylene glycol (PEG) or other polymers or polyelectrolytes and, finally, with tightly grafted plasma proteins (albumin or apolipoprotein E). All AuNP had been radioactively labeled with 198Au by neutron activation in a nuclear research reactor. Most of these studies were

Non-covalent and reversible functionalization of carbon nanotubes

• Antonello Di Crescenzo,
• Valeria Ettorre and
• Antonella Fontana

Beilstein J. Nanotechnol. 2014, 5, 1675–1690, doi:10.3762/bjnano.5.178

• still under investigation. Polyethylene glycol (PEG) chains with PEG repeating units as long as 8, have been demonstrated [52] to favor both self-assembly and dispersion of SWCNTs in chloroform and 1,2-dichlorobenzene once chosen a hydrophobic domain B (i.e., hexa-p-phenylene) able to establish π–π

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

• Moritz Nazarenus,
• Qian Zhang,
• Mahmoud G. Soliman,
• Pablo del Pino,
• Beatriz Pelaz,
• Susana Carregal-Romero,
• Joanna Rejman,
• Barbara Rothen-Rutishauser,
• Martin J. D. Clift,
• Reinhard Zellner,
• G. Ulrich Nienhaus,
• James B. Delehanty,
• Igor L. Medintz and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161

• efficiently [34]. Non-fouling polyethylene glycol (PEG)-modified NPs, for example, stick less to the cell plasma membrane and are, therefore, incorporated by cells less efficiently than other NPs [35][36][37] (this is also true in vivo as manifested by enhanced retention times [38]). It is clear that, while

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

• Paula M. Castillo,
• Mario de la Mata,
• Maria F. Casula,
• José A. Sánchez-Alcázar and
• Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

• surface modification of the nanoparticles by polyethylene glycol enables loading a large amount of camptothecin. While the unloaded nanoparticles do not induce apoptosis in the H460 lung cancer cell line, the camptothecin-loaded nanoparticle formulations exhibit remarkable pro-apoptotic activity. These

• ; cancer therapy; iron oxide superparamagnetic nanoparticles; polyethylene glycol; Introduction Camptothecin (CPT) is a quinoline based alkaloid, which exhibits a potent cytotoxic activity against a broad spectrum of tumours [1][2][3]. While most antineoplastic agents inhibit cancer cell proliferation by

• polyethylene glycol (PEG) as coating polymer in nano-formulations. PEG has been widely used in the formulation of nanoparticles for biomedical applications, both because of its biocompatibility and its effectiveness in camouflaging nanoparticles from opsonins [35]. Recently, it has been described that PEG

Preparation of poly(N-vinylpyrrolidone)-stabilized ZnO colloid nanoparticles

• Tatyana Gutul,
• Emil Rusu,
• Nadejda Condur,
• Veaceslav Ursaki,
• Evgenii Goncearenco and
• Paulina Vlazan

Beilstein J. Nanotechnol. 2014, 5, 402–406, doi:10.3762/bjnano.5.47

• widely investigated in recent years in connection with a possible employment in biology [6]. ZnO nanoparticles have been synthesized in conjunction with different polymers such as polyethylene glycol (PEG) and poly(N-vinylpyrrolidone) (PVP). Nanoparticles of various morphologies were formed, and the

One pot synthesis of silver nanoparticles using a cyclodextrin containing polymer as reductant and stabilizer

• Arkadius Maciollek and
• Helmut Ritter

Beilstein J. Nanotechnol. 2014, 5, 380–385, doi:10.3762/bjnano.5.44

• described which act simultaneously as reducing and stabilization agent in aqueous medium. Typical examples are polyacrylamide, poly(sodium acrylate), polyethylene glycol, chitosan or soluble starch [11][17][18][19]. However, up to now polymers bearing CD attached have not been used for the preparation of

Study of mesoporous CdS-quantum-dot-sensitized TiO₂ films by using X-ray photoelectron spectroscopy and AFM

• Mohamed N. Ghazzal,
• Robert Wojcieszak,
• Gijo Raj and
• Eric M. Gaigneaux

Beilstein J. Nanotechnol. 2014, 5, 68–76, doi:10.3762/bjnano.5.6

• solution was obtained by the addition of dissolved polyethylene glycol hexadecyl ether (denoted Brij 56, C16H33(OCH2CH2)nOH, n ≈ 10, Aldrich) into 1-butanol (BuOH, >99.4%, Alfa Aesar). The final molar ratio of the solution was TEOT/HCl/1-butanol/Brij 56 1:2–4:9:0.05. The solutions were subsequently aged

Energy transfer in complexes of water-soluble quantum dots and chlorin e6 molecules in different environments

• Irina V. Martynenko,
• Anna O. Orlova,
• Vladimir G. Maslov,
• Alexander V. Baranov,
• Anatoly V. Fedorov and
• Mikhail Artemyev

Beilstein J. Nanotechnol. 2013, 4, 895–902, doi:10.3762/bjnano.4.101

• -terminated polyethylene glycol (PEG-OH)thiol and amino-terminated polyethylene glycol (PEG-NH2)thiol with a ratio of 3 to 1. This enables to obtain stable colloidal solutions of quantum dots. Then the covalent binding of the QD surface amino groups with the Ce6 carboxyl functional groups using EDAC as a

Polymer blend lithography: A versatile method to fabricate nanopatterned self-assembled monolayers

• Cheng Huang,
• Markus Moosmann,
• Jiehong Jin,
• Tobias Heiler,
• Stefan Walheim and
• Thomas Schimmel

Beilstein J. Nanotechnol. 2012, 3, 620–628, doi:10.3762/bjnano.3.71

• platform for constructive lithography [1], cell adhesion studies, or the study of other template-induced phenomena. The FDTS-SAM could be replaced by other silanes, such as octadecyltrichlorosilane (OTS) or polyethylene glycol (PEG) silane, for desired applications [63][64]. The bare silicon surface at the

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• biocompatible hydrophilic functional groups to SN38 through chemical modification. A 40 kDa polyethylene glycol has been linked to the SN38 [16]. The highly water-soluble PEGylated SN38 (EZN-2208) demonstrated both drastic enhancement of its circulating half-life and preferential accumulation in solid tumors

• dopamine to SN38 Dopamine has been reported as a robust anchor to immobilize functional groups on the surface of iron oxide nanoparticles [39][40][41][42][43][44][45]. Introducing polyethylene glycol to the dopamine anchor can greatly improve both the solubility and biocompatibility of iron oxide

Sensing surface PEGylation with microcantilevers

• Natalija Backmann,
• Natascha Kappeler,
• Thomas Braun,
• François Huber,
• Hans-Peter Lang,
• Christoph Gerber and
• Roderick Y. H. Lim

Beilstein J. Nanotechnol. 2010, 1, 3–13, doi:10.3762/bjnano.1.2

• example is polyethylene glycol (PEG), which is widely used as a protein-resistant coating given its low toxicity and biocompatibility. We report here a microcantilever-based sensor for the in situ characterization of PEG monolayer formation on Au using the “grafting to” approach. Moreover, we demonstrate

• reversible collapse when switching between good and poor solvent conditions, respectively. Keywords: AFM; cantilever sensor; polyethylene glycol; polymer brush; reversible collapse; static mode; Introduction Polyethylene glycol (PEG) is often used as a protein-resistant surface layer in biomedicine and

• (polyethylene glycol) thiol 20000 (mPEG–SH, MW ~ 20000) was purchased from Laysan Bio Inc (Arab, AL). (1-Mercapto-11-undecyl)tetra(ethylene glycol) (EG4–C11–SH, MW ~ 380.5) was obtained from Asemblon Inc (Seattle, WA), diluted in ethanol to an end concentration of 10 mM and stored at +4 °C. Coating of