Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

• derived products cannot provide adequate amounts of active VB12 for the human body, the supplementation of plant products such as breakfast cereals and nondairy milk is considered a possible dietary strategy for preventing its deficiency among vegetarians and vegans [2][6][7]. The deficiency of VB12 can

• + concentrations from 1.0 × 10−9 to 3.0 × 10−7 g/mL and an LOD of 4.4 × 10−10 g/mL with 2.3% RSD [105]. Andrade et al. designed a flow–batch methodology paired with a large-area silicon photodiode instead of a photomultiplier tube, resulting in a convenient and automated luminometer for CL analysis. In this

Electromagnetic study of a split-ring resonator metamaterial with cold-electron bolometers

• Ekaterina A. Matrozova,
• Alexander V. Chiginev,
• Leonid S. Revin and
• Andrey L. Pankratov

Beilstein J. Nanotechnol. 2025, 16, 2199–2206, doi:10.3762/bjnano.16.152

• Ekaterina A. Matrozova Alexander V. Chiginev Leonid S. Revin Andrey L. Pankratov Nizhny Novgorod State Technical University n.a. R.E. Alekseev, MininStreet, 24, Nizhny Novgorod, 603155, Russia Institute for Physics of Microstructures of the Russian Academy of Sciences, Akademicheskaya Street, 7

• , enabling operation in a wide frequency range from gigahertz frequencies to X-rays [6][7][8] due to a normal-metal absorber. CEBs offer several advantages over other types of receivers such as transition edge sensors [9][10][11]. These advantages include their micrometer-scale size, which facilitates direct

• increase in the absorbed power (Figure 5). Figure 5 shows the AFC of the SRR metamaterial with a different number of elements. For the large-scale design (period P = 86 μm, rings: dout,1/din,1 = 80/70 μm, dout,2/din,2 = 40/30 μm), doubling the number of elements increases the absorbed power by about 7

Ultrathin water layers on mannosylated gold nanoparticles

• Maiara A. Iriarte Alonso,
• Jorge H. Melillo,
• Silvina Cerveny,
• Yujin Tong and
• Alexander M. Bittner

Beilstein J. Nanotechnol. 2025, 16, 2183–2198, doi:10.3762/bjnano.16.151

• properties on particle size and shape has been demonstrated for particle sizes in the 1–100 nm range and on biological interfaces [7]. Limited biocompatibility and high tendency to aggregate in solution inspired new mechanisms of particle biofunctionalization with proteins, lipids, or carbohydrates. Coupling

• high and the shape cannot be identical (hemagglutinin is roughly a triangular 7 nm prism of 15 nm length), the size is in the typical NP range, and a dense coating with oligomannoside should mimic surface physics. Accordingly, we note that the adsorption of AuNPs on surfaces would mimic the survival of

• -AuNPs. In both cases, the NP surface charge in water at pH ~7 was approx. −20 mV. The isoelectric point of carboxylate PEG-capped particles is ~2.5; hence, around pH 7, they should exhibit a negative ZP. This is also compatible with a low carboxylate content, as citrate-capped AuNPs (with a higher

Hartree–Fock interaction in superconducting condensate fractals

• Edward G. Nikonov,
• Yajiang Chen,
• Mauro M. Doria and
• Arkady A. Shanenko

Beilstein J. Nanotechnol. 2025, 16, 2177–2182, doi:10.3762/bjnano.16.150

• ]. Nevertheless, the HF potential cannot be neglected in the presence of external fields [4], such as impurity potentials [5][6], quantum confinement in nanoscale superconductors [7], and potential barriers at interfaces [8]. Such external fields break the translational invariance, which is the condition for the

• the quasiparticle states in ascending energy order. In our study, we employ the open-boundary conditions [5][8][22] for the quasiparticle wavefunctions uν(i) and vν(i), which corresponds to the physical scenario of electrons being quantum-confined within the chain. When using Equation 5 and Equation 7

• the entire procedure is repeated until convergence is achieved. The calculation is considered converged when the relative changes in the order parameter and the HF field are below 10−7. To model quasicrystal superconducting properties, as the first step, we consider a finite Fibonacci sequence

Missing links in nanomaterials research impacting productivity and perceptions

• Santosh K. Tiwari and
• Nannan Wang

Beilstein J. Nanotechnol. 2025, 16, 2168–2176, doi:10.3762/bjnano.16.149

• acceleration after the discovery of fullerene by Kroto and co-workers in 1985 and witnessed an even more rapid surge following the discovery of graphene and the award of the Physics Nobel Prize in 2010 [6][7]. Graphene is often referred to as a “wonder material” due to its seemingly infinite potential in

• capital by training engineers specialized in nanotechnology, while nurturing interdisciplinary research to drive fundamental discoveries. (7) Integration of artificial intelligence, machine learning, and digital twins can accelerate materials discovery, predict safety and performance, and guide scale-up

Microplastic pollution in Himalayan lakes: assessment, risks, and sustainable remediation strategies

• Sameeksha Rawat,
• S. M. Tauseef and
• Madhuben Sharma

Beilstein J. Nanotechnol. 2025, 16, 2144–2167, doi:10.3762/bjnano.16.148

• to MP pollution [4][7][8][9]. MPs enter these ecosystems due to glacier melting, tourism, agricultural runoff, and inadequate waste management. Anchar Lake and Dal Lake in Kashmir, for instance, exhibit high MP levels owing to household waste and touristic activities [10]. Recreational activities and

• key mechanism in remote lakes besides direct input [7]. Groundwater modeling methods have been instrumental in describing the transport and fate of contaminants, including MPs, in subsurface environments. Numerical methods such as MODFLOW coupled with transport methods such as MT3DMS and RT3D are

• climates represent a promising direction for future trials. 7 Ecotoxicological impact and risk assessment MP pollution of high-altitude ecosystems is a new issue with significant ecological and health effects. Because of low temperature, high levels of UV irradiation, and slow biodegradation rates, MPs

Electron transport through nanoscale multilayer graphene and hexagonal boron nitride junctions

• Aleksandar Staykov and
• Takaya Fujisaki

Beilstein J. Nanotechnol. 2025, 16, 2132–2143, doi:10.3762/bjnano.16.147

• sites and hinders the electron mobility [7]. In addition, zigzag and armchair edges in graphene nanoribbons will open the bandgap and create trapping sites. Thus, small graphene nanoribbons will show higher electrical resistance compared to larger nanoribbons and infinite two-dimensional sheets. The in

• defects in two-dimensional graphene, that is, N-substitution of carbon atoms and Stone–Wales defects [7]. Nitrogen substitution leads to three distinct states, namely, pyridinic, pyrrolic, and graphitic [14]. While pyridinic and pyrrolic nitrogen involves graphene edges, the graphitic nitrogen can be seen

• as a bulk defect. In this work, we deal with periodic graphene models, and we consider only graphitic nitrogen substitutions. The graphitic nitrogen substitution in graphene changes its properties from a zero-bandgap semiconductor to metallic character and decreases its work function [7]. Stone–Wales

Quality by design optimization of microemulsions for topical delivery of Passiflora setacea seed oil

• Daniel T. Pereira,
• Douglas Dourado,
• Danielle T. Freire,
• Dayanne L. Porto,
• Cícero F. S. Aragão,
• Myla L. de Souza,
• Guilherme R. S. de Araujo,
• Ana Maria Costa,
• Wógenes N. Oliveira,
• Anne Sapin-Minet,
• Éverton N. Alencar and
• Eryvaldo Sócrates T. Egito

Beilstein J. Nanotechnol. 2025, 16, 2116–2131, doi:10.3762/bjnano.16.146

• solubilization capacity, ability to enhance dermal permeation, and cost-effectiveness make microemulsions attractive carriers compared with other delivery systems [6][7]. Nevertheless, conventional microemulsions typically require high concentrations of surfactants, which may raise safety concerns such as

• of the physicochemical properties and interactions of their constituents [7][9]. In this context, the quality by design (QbD) framework provides a systematic and scientifically grounded approach for pharmaceutical formulation development. QbD emphasizes predefined quality objectives, product and

• effects of OPS concentration, the surfactants mixture ratio (Smix), and Smix concentration on the formation of a microemulsified system with desirable hydrodynamic diameter and transparency (Table 7). Initially, a full factorial design (23) with three central points was employed to explore the

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• study is the first to investigate the cytotoxic effects of these NCs on human breast adenocarcinoma (MCF-7), colon cancer cell lines (HT-29), and fibroblast cell lines (L929). Additionally, the antibacterial properties of the NCs against gram-positive (Staphylococcus aureus and Bacillus subtilis) and

• , particularly on cancer cell lines (MCF-7 and HT-29), in a dose-dependent manner over a 24 h period. These findings highlight the potential of the NCs as anticancer agents. Furthermore, the synthesized NCs demonstrated potent antibacterial properties against the tested microorganisms and notable antioxidant

• from antibacterial activity to tumor therapy activity [5][6][7]. The escalating global concern regarding antimicrobial resistance presents a considerable threat to public health. Researchers are persistently exploring alternative strategies to address this pressing issue and the critical demand for

Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation

• Michał Bartkowski,
• Francesco Calzaferri and
• Silvia Giordani

Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144

• energy sectors (Figure 1). NMs have seen use as antimicrobial agents [1], catalysts [2], bioimaging agents [3][4][5][6], magnetic particle imaging agents [7], nanofluids [8], antiviral agents [9], photothermal convertors [10], and in environmental remediation [11]. Topically, the biomedical applications

Calibration of piezo actuators and systems by dynamic interferometry

• Knarik Khachatryan and
• Michael Reichling

Beilstein J. Nanotechnol. 2025, 16, 2086–2091, doi:10.3762/bjnano.16.143

• Knarik Khachatryan Michael Reichling Institut für Physik, Universität Osnabrück, Barbarastr. 7, 49076 Osnabrück, Germany 10.3762/bjnano.16.143 Abstract To achieve precise measurements of small displacements in non-contact atomic force microscopy, it is crucial to control the position of moving

• microscopy (NC-AFM) operation in an ultrahigh-vacuum (UHV) environment at low temperature and has been developed tremendously over the last three decades [7][8][9][10][11][12]. In frequency-modulated NC-AFM, the cantilever is kept at oscillation with constant amplitude, yielding an interferometric signal

• interferometer, various voltages are applied to the fiber tube piezo, and, after each step of voltage change, the cantilever excitation voltage is varied from 1 V to 7 V in steps. For each step, the interferometer signal is analyzed to extract the oscillation amplitude A corresponding to the respective voltage

Multifrequency AFM integrating PeakForce tapping and higher eigenmodes for heterogeneous surface characterization

• Yanping Wei,
• Jiafeng Shen,
• Yirong Yao,
• Xuke Li,
• Ming Li and
• Peiling Ke

Beilstein J. Nanotechnol. 2025, 16, 2077–2085, doi:10.3762/bjnano.16.142

• interacting intermittently with the sample surface. By monitoring and controlling the cantilever’s amplitude and phase, topographical and phase images can be generated, providing insights into material properties and enabling the differentiation of regions or components within heterogeneous samples [7][8][9

Molecular and mechanical insights into gecko seta adhesion: multiscale simulations combining molecular dynamics and the finite element method

• Yash Jain,
• Saeed Norouzi,
• Tobias Materzok,
• Stanislav N. Gorb and
• Florian Müller-Plathe

Beilstein J. Nanotechnol. 2025, 16, 2055–2076, doi:10.3762/bjnano.16.141

• structures on their feet [1][2]. This bioadhesion mechanism has been studied extensively, especially for biomimetic adhesive applications [3][4][5][6][7][8][9]. Understanding these interactions presents a formidable challenge in biophysics and materials science due to the extremely small length and time

• multiscale model, there were 16 bridging domains (BDs), one for each spatula. Each BD needed to be of a size that could be described by both (MD and FEM) methods. The FE portion of each BD was roughly 79 × 75 × 50 nm3 in size, and the overlapping MD portion was roughly 40 × 36 × 39 nm3 (Figure 7). The number

• springs with stiffness (FE-coupling spring constant) 0.08 nN/nm. Figure 7 illustrates a typical BD configuration, showing the overlapping domains, anchor points, and harmonic spring connections. Simulation Details and Parameters Iteration details Molecular dynamics We used the velocity-Verlet algorithm

Stereodiscrimination of guests in chiral organosilica aerogels studied by ESR spectroscopy

• Sebastian Polarz,
• Yasar Krysiak,
• Martin Wessig and
• Florian Kuhlmann

Beilstein J. Nanotechnol. 2025, 16, 2034–2054, doi:10.3762/bjnano.16.140

• , chiral materials are crucial in asymmetric synthesis, where the selective production of a desired enantiomer is needed, particularly in pharmaceuticals [7]. Chiral metal-organic frameworks [8] and nanoporous materials [9][10] are emerging as vital components in enantioselective catalysis due to their

Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• advancements in the controlled release of diverse bioactive compounds [5][6][7][8][9][10]. Among the various nanostructured platforms explored for these purposes, nanofibers have gained attention due to their high surface area, adjustable porosity, and robust mechanical properties, which set them apart from

• profile can be modulated, and the initial burst release minimized [7]. Beyond drug delivery, an ideal biomedical scaffold should support cellular attachment while providing effective drug release; balancing these functions is crucial for promoting tissue formation. Achieving this balance in electrospun

• -FTIR analysis was performed on PLA (monolithic), HA/PLA, and HA+NE2/PLA nanofiber samples, along with pure HA powder. The obtained spectra (Figure 7) confirmed effective shell coating, as only the characteristic bands of PLA were detected in the core–shell nanofibers. All three nanofiber membranes (NF

The cement of the tube-dwelling polychaete Sabellaria alveolata: a complex composite adhesive material

• Emilie Duthoo,
• Aurélie Lambert,
• Pierre Becker,
• Carla Pugliese,
• Jean-Marc Baele,
• Arnaud Delfairière,
• Matthew J. Harrington and
• Patrick Flammang

Beilstein J. Nanotechnol. 2025, 16, 1998–2014, doi:10.3762/bjnano.16.138

• tube-dwelling worms that build their tube using a specialized building organ located near the mouth. The two finger-like lobes of this organ allow them to manipulate sand grains or shell fragments and to glue them together with several spots of a strong proteinaceous cement [6][7][8][9]. The building

• sequences from P. californica proteins were used as queries for BLAST searches in the transcriptome of S. alveolata to identify new adhesive proteins. However, as previous studies have shown, limited percentages of identity in the alignment of these proteins made it difficult to find homologues [7][31

• . alveolata definitively confirms the presence of two types of cement cells in S. alveolata, namely, cement cells containing homogeneous granules and those containing heterogeneous granules (Figure 7). These cells are located in the three parathoracic segments, around the digestive tract and at the base of

Laser ablation in liquids for shape-tailored synthesis of nanomaterials: status and challenges

• Natalie Tarasenka

Beilstein J. Nanotechnol. 2025, 16, 1963–1997, doi:10.3762/bjnano.16.137

• . Combining both top-down and bottom-up strategies, laser-assisted methods are demonstrating the prospects to become a versatile nanoscale manufacturing strategy based on clean, sustainable, and large-scale approach applicable to a broad range of nanomaterials [1][2][3][4][5][6][7][8][9][10][11]. The current

• closer to the target surface. It is typically accepted that release of NPs from the cavitation bubble into a liquid occurs during later stages of cavitation bubble collapse. The latest reports by Dell’Aglio et al. [7], however, demonstrate that particle ejection into a liquid occurs mainly in two steps

• : in the beginning of the bubble expansion stage, and in the period between bubble collapse and rebound [7]. The major part of the ejected material is released during the second stage of cavitation bubble collapse. The charging of NPs has an impact at this stage, changing the electrostatic pressure

Mechanical property measurements enabled by short-term Fourier-transform of atomic force microscopy thermal deflection analysis

• Thomas Mathias,
• Roland Bennewitz and
• Philip Egberts

Beilstein J. Nanotechnol. 2025, 16, 1952–1962, doi:10.3762/bjnano.16.136

• of the overall structure. Analytical models for interpreting the vibrational modes of cantilevers were developed prior to the invention of the technique [7][8]. This model or variations of it are often presented in manuscripts to explain the interpretation of experimental data, but are not used to

• quantitative measurements from the frequency of the AFM cantilever’s bending mode. In this manuscript, we bring together the analytical models that describe cantilever oscillations in AFM experiments where a tip is oscillated and pressed into contact with a solid surface [7][8] with the spectral analysis of

• models originating from the work by Rabe et al. [7], and are schematically shown in Figure 1a(i) and Figure 1a(ii). More advanced models that include the tilt angle of the cantilever relative to the surface [23], to better reflect the typical 12.5° or 22.5° angles of the cantilever relative to the

Evaluating metal-organic precursors for focused ion beam-induced deposition through solid-layer decomposition analysis

• Benedykt R. Jany,
• Katarzyna Madajska,
• Aleksandra Butrymowicz-Kubiak,
• Franciszek Krok and
• Iwona B. Szymańska

Beilstein J. Nanotechnol. 2025, 16, 1942–1951, doi:10.3762/bjnano.16.135

• Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland 10.3762/bjnano.16.135 Abstract The development of modern metal deposition techniques like focused ion/electron beam-induced deposition (FIBID/FEBID) relies heavily on the availability of metal-organic precursors of particular properties. To

• or ion beam-induced deposition (FEBID/FIBID) allows for the precise fabrication of two- and three-dimensional nanostructures with well-defined shapes and dimensions ranging from 5 to 10 nm [1][5][6][7][8][9][10]. This high spatial resolution is achieved by precisely controlling the position and

• technique, which is a high-resolution analytical method that can provide elemental information at the nanoscale. This step ensures precise identification and quantification of all elements present in the grown structures. (7) The final step involves examining the chemical composition of the resulted

Quantum circuits with SINIS structures

• Mikhail Tarasov,
• Mikhail Fominskii,
• Aleksandra Gunbina,
• Artem Krasilnikov,
• Maria Mansfeld,
• Dmitrii Kukushkin,
• Andrei Maruhno,
• Valeria Ievleva,
• Mikhail Strelkov,
• Daniil Zhogov,
• Konstantin Arutyunov,
• Vyacheslav Vdovin,
• Vladislav Stolyarov and
• Valerian Edelman

Beilstein J. Nanotechnol. 2025, 16, 1931–1941, doi:10.3762/bjnano.16.134

• devices are manufactured on their basis [1][2][3]. These extend from cryogenic thermometers [4][5][6] and electron coolers [7][8][9][10] to various detectors such as Andreev bolometers [11][12][13], cold electron bolometers [14][15], superconductor–insulator–normal metal–insulator–superconductor (SINIS

• thermometers is the ratio of the resistance at zero bias to the asymptotic one (Equation 7): obtained from the general relation in Equation 3. For I(V) = I0exp[(eV − Δ)/kTe] the temperature sensitivity at low bias current can be approximated as dV/dTe = (k/e)ln(I/I0) ≈ −VΔ/Te. To reduce the influence of

• measurements with a room-temperature JFET readout system and can be improved by upgrading the readout electronics). As an example, some measured characteristics of the SINIS detectors are given in Figure 7. The amplifier voltage noise is 20 nV·Hz−1/2, the optimal input impedance for this amplifier is 500 kΩ

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• strategies in LNP-based drug delivery systems, approaches are being explored. These include integrating functional groups into PEG lipids for ligand conjugation and improved cell-specific targeting, as well as developing PEG alternatives to mitigate anti-PEG antibody associated immunogenicity [7][8]. This

• molar ratio of 50:10:38.5:1.5, SANS analysis revealed a core–shell architecture with an average particle diameter of approximately 75 nm and a LNP shell thickness of 5 to 7 nm. Quantitative modeling of shell composition further demonstrated that the DMPE-PEG2k lipid accounted for 3.0 ± 0.5% of the shell

• volume, alongside 38 ± 7% MC3, 33 ± 3% cholesterol, and 26 ± 4% DSPC. Notably, PEG lipids were found exclusively in the shell region, with no detectable presence in the core area [10]. This localization of PEG lipids aligns with their intended function to form a sterically repulsive layer that stabilizes

Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties

• Laryssa Ferreira do Nascimento Silva,
• Douglas Dourado,
• Thayse Silva Medeiros,
• Mariana Alice Gonzaga Gabú,
• Maria Cecilia Queiroga dos Santos,
• Daiane Rodrigues dos Santos,
• Mylena Lemos dos Santos,
• Gabriel Bezerra Faierstein,
• Rosângela Maria Rodrigues Barbosa and
• Fabio Rocha Formiga

Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132

• vector competence [5][6]. However, Ae. albopictus has been reported as the main or even the sole vector responsible for arbovirus transmission in certain regions of the world, demonstrating significant epidemiological relevance under specific ecological conditions [7]. Therefore, these arthropods play a

Programmable soliton dynamics in all-Josephson-junction logic cells and networks

• Vsevolod I. Ruzhickiy,
• Anastasia A. Maksimovskaya,
• Sergey V. Bakurskiy,
• Andrey E. Schegolev,
• Maxim V. Tereshonok,
• Mikhail Yu. Kupriyanov,
• Nikolay V. Klenov and
• Igor I. Soloviev

Beilstein J. Nanotechnol. 2025, 16, 1883–1893, doi:10.3762/bjnano.16.131

• ; superconducting neural networks; Introduction The rapid advancement of Josephson junction (JJ) logic circuits [1][2][3][4][5] and neuromorphic networks [6][7][8][9] holds transformative potential for ultra-low-power computing. However, achieving scalable integration remains a critical bottleneck, as conventional

• , which can be represented in matrix form: The resulting system of N ordinary differential equations is expressed in the matrix form shown in Equation 7. In this equation, is the vector of nodal phases, N is the total number of non-ground nodes, and is the N × N mass matrix (also known as the

Low-temperature AFM with a microwave cavity optomechanical transducer

• Ermes Scarano,
• Elisabet K. Arvidsson,
• August K. Roos,
• Erik Holmgren,
• Riccardo Borgani,
• Mats O. Tholén and
• David B. Haviland

Beilstein J. Nanotechnol. 2025, 16, 1873–1882, doi:10.3762/bjnano.16.130

• ], tests of quantum gravity [3][4][5], force microscopy [6][7][8], magnetometry [9][10], and quantum state transfer [11][12][13]. In some cases, further improvement on fundamental figures of merit is required, while, in other cases, the difficulty lies in balancing trade-offs to find an optimal design that

Self-assembly and adhesive properties of Pollicipes pollicipes barnacle cement protein cp19k: influence of pH and ionic strength

• Shrutika Sawant,
• Anne Marie Power and
• J. Gerard Wall

Beilstein J. Nanotechnol. 2025, 16, 1863–1872, doi:10.3762/bjnano.16.129

• bond formation, metal chelation, and covalent interactions [6]. The byssal threads display a mechanical gradient, with regions proximal to the animal more elastic and distal regions stiffer and tougher [7]. This design allows for effective dissipation of hydrodynamic forces and maintains attachment of

• the threads under wave-induced stress, thereby allowing the byssus to function as both an adhesive and a shock-absorbing tether [7]. Mussel adhesives are difficult to re-create either synthetically or recombinantly [8], the latter due largely to difficulties associated with post-translational