Biocatalytic synthesis of the Green Note trans-2-hexenal in a continuous-flow microreactor

Scholarly Works

• Ascaso-Alegre, C.; Cinca-Fernando, P.; Roberts, T. L.; López-Fernández, P.; Herrera, R. P.; Cosgrove, S. C.; Ferreira, P.; Mangas-Sánchez, J. Scope and Synthetic Applications of the Aryl-Alcohol Oxidase from Streptomyces hiroshimensis (ShAAO). Organic letters 2025, 27, 12086–12091. doi:10.1021/acs.orglett.5c03814
• Bai, J.; Huang, C.; Liu, Y.; Zheng, X.; Liu, J.; Zhou, L.; Liu, J.; Jiang, Y. Integrating biocatalysis with continuous flow: current status, challenges, and future perspectives. Journal of advanced research 2025. doi:10.1016/j.jare.2025.07.010
• Cinca-Fernando, P.; Vázquez-Rodríguez, A.; Mangas-Sánchez, J.; Ferreira, P. Aryl-alcohol oxidases: catalysis, diversity, structure-function and emerging biotechnological applications. Applied microbiology and biotechnology 2025, 109, 151. doi:10.1007/s00253-025-13538-7
• Yang, Y.-L. Mushroom-Mediated Redox Reactions. Chemistry (Weinheim an der Bergstrasse, Germany) 2024, 31, e202403010. doi:10.1002/chem.202403010
• Cinca-Fernando, P.; Ascaso-Alegre, C.; Sevilla, E.; Martínez-Júlvez, M.; Mangas-Sánchez, J.; Ferreira, P. Discovery, characterization, and synthetic potential of two novel bacterial aryl-alcohol oxidases. Applied microbiology and biotechnology 2024, 108, 498. doi:10.1007/s00253-024-13314-z
• Cinca-Fernando, P.; Ascaso-Alegre, C.; Sevilla, E.; Martínez-Júlvez, M.; Mangas-Sánchez, J.; Ferreira, P. Discovery, Characterization and Synthetic Potential of Two Novel Bacterial Alcohol Oxidases. Cold Spring Harbor Laboratory 2024. doi:10.1101/2024.03.22.586125
• Bora, P. K.; Kemprai, P.; Hussain, S.; Baishya, R.; Jadhav, D.; Haldar, S. Mushroom‐based biocatalysts for the synthesis of aroma and flavours from exogenous organic molecules: a review of two decades. International Journal of Food Science & Technology 2023, 59, 84–94. doi:10.1111/ijfs.16829
• Babkina, V. V.; Albuquerque, W.; Haiduk, Y. M.; Michalak, W.; Ghezellou, P.; Zorn, H.; Zhuk, T. S. Fungal lyophilisates as catalysts for organic synthesis: Preparative oxidations with the white-rot fungus Bjerkandera adusta. Molecular Catalysis 2023, 549, 113451. doi:10.1016/j.mcat.2023.113451
• Schober, L.; Dobiašová, H.; Jurkaš, V.; Parmeggiani, F.; Rudroff, F.; Winkler, M. Enzymatic reactions towards aldehydes: An overview. Flavour and fragrance journal 2023, 38, 221–242. doi:10.1002/ffj.3739
• Hecko, S.; Schiefer, A.; Badenhorst, C. P. S.; Fink, M. J.; Mihovilovic, M. D.; Bornscheuer, U. T.; Rudroff, F. Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity. Chemical reviews 2023, 123, 2832–2901. doi:10.1021/acs.chemrev.2c00304
• Ma, Y.; Li, Z.; Zhang, H.; Wong, V. K. W.; Hollmann, F.; Wang, Y. Bienzymatic Cascade Combining a Peroxygenase with an Oxidase for the Synthesis of Aromatic Aldehydes from Benzyl Alcohols. Catalysts 2023, 13, 145. doi:10.3390/catal13010145
• Kon, Y.; Nakashima, T.; Onozawa, S.; Sato, K.; Kobayashi, S. Switchable Synthesis of Aldehydes and Carboxylic Acids from Alcohols by Platinum‐Catalysed Hydrogen Peroxide Oxidation Using Flow Reactors. Advanced Synthesis & Catalysis 2022, 364, 3372–3377. doi:10.1002/adsc.202200632
• Jankowski, N.; Koschorreck, K.; Urlacher, V. B. Aryl‐Alcohol‐Oxidase‐Mediated Synthesis of Piperonal and Other Valuable Aldehydes. Advanced Synthesis & Catalysis 2022, 364, 2364–2372. doi:10.1002/adsc.202200381
• Heath, R. S.; Ruscoe, R. E.; Turner, N. J. The beauty of biocatalysis: sustainable synthesis of ingredients in cosmetics. Natural product reports 2022, 39, 335–388. doi:10.1039/d1np00027f
• Lorente-Arevalo, A.; Ladero, M.; Bolivar, J. M. Intensification of oxygen-dependent biotransformations catalyzed by immobilized enzymes. Current Opinion in Green and Sustainable Chemistry 2021, 32, 100544. doi:10.1016/j.cogsc.2021.100544
• Lappe, A.; Jankowski, N.; Albrecht, A.; Koschorreck, K. Characterization of a thermotolerant aryl-alcohol oxidase from Moesziomyces antarcticus oxidizing 5-hydroxymethyl-2-furancarboxylic acid. Applied microbiology and biotechnology 2021, 105, 8313–8327. doi:10.1007/s00253-021-11557-8
• Naramittanakul, A.; Buttranon, S.; Petchsuk, A.; Chaiyen, P.; Weeranoppanant, N. Development of a continuous-flow system with immobilized biocatalysts towards sustainable bioprocessing. Reaction Chemistry & Engineering 2021, 6, 1771–1790. doi:10.1039/d1re00189b
• Jankowski, N.; Urlacher, V. B.; Koschorreck, K. Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris. Applied microbiology and biotechnology 2021, 105, 7743–7755. doi:10.1007/s00253-021-11585-4
• Sánchez-Ruiz, M. I.; Martínez, Á. T.; Serrano, A. Optimizing operational parameters for the enzymatic production of furandicarboxylic acid building block. Microbial cell factories 2021, 20, 180. doi:10.1186/s12934-021-01669-1
• Urlacher, V. B.; Koschorreck, K. Pecularities and applications of aryl-alcohol oxidases from fungi. Applied microbiology and biotechnology 2021, 105, 4111–4126. doi:10.1007/s00253-021-11337-4

Explore citations to this article at