Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

• Shijie Ye,
• Allison Ann Berger,
• Dominique Petzold,
• Oliver Reimann,
• Benjamin Matt and
• Beate Koksch

Beilstein J. Org. Chem. 2010, 6, No. 40, doi:10.3762/bjoc.6.40

• ][25][26][27]. We chose a combination of chemical and enzymatic aminoacylation which relies on the hybrid dinucleotide pdCpA and the T4-RNA ligase-mediated coupling to it to give a truncated suppressor tRNA. The Nα-amino groups of the amino acids were protected with the 4-pentenoyl group and the amino

• and incorporated into proteins [29][30]. pdCpA bearing Abu or the fluorinated amino acid analogue were efficiently ligated to the truncated suppressor tRNAPheCUA by treatment with T4-RNA ligase and were analyzed using denatured acidic PAGE (Figure 3) [31]. Thus, both our mono- and bis-acylated tRNAs

• . (RS)-2-amino-2-methyl-3,3,3-trifluoropropanoic acid. Denaturing PAGE of ligation products of truncated suppressor tRNA and fluorinated aminoacyl-pdCpAs and Abu-pdCpA. Lane 1: RNA Marker 100 bp, Lane 2: transcribed full-length tRNACUA, Lane 3: transcribed truncated tRNACUA-COH, Lane 4: Abu-tRNACUA

(Pseudo)amide-linked oligosaccharide mimetics: molecular recognition and supramolecular properties

• José L. Jiménez Blanco,
• Fernando Ortega-Caballero,
• Carmen Ortiz Mellet and
• José M. García Fernández

Beilstein J. Org. Chem. 2010, 6, No. 20, doi:10.3762/bjoc.6.20

• other, a high degree of diversity can be achieved by employing only a small set of different SDAs. Moreover, oligomeric SDAs with unprotected groups represent a novel type of aminoglycoside mimetics with potential recognition properties towards new RNA targets emerging in the post-genome era. Cyclic SAA

• negatively charged functional groups such as phosphate or carboxylate groups in DNA, RNA and proteins. Tóth and co-workers [90] synthesised a series of guanidine-linked sugars as a new class of oligosaccharide mimics to study their interactions with proteins. Thus, reaction of the carbodiimide-linked

• [83][84]. Numerous structural analogues of DNA/RNA designed to be effective antisense/antigene agents have been reported. An interesting approach involves replacing the negatively charged phosphodiester linkages of RNA/DNA by positively charged guanidinium linkages. The guanidinium linkage is

A hydrogen- bonded channel structure formed by a complex of uracil and melamine

• Reji Thomas and
• G. U. Kulkarni

Beilstein J. Org. Chem. 2007, 3, No. 17, doi:10.1186/1860-5397-3-17

• channel structure with 4,4'-bipyridyl. [5] Although hydrogen bonding aspects of cyanuric acid have been studied in detail, similar structures formed by uracil possessing two imide groups has not been studied adequately, except for its hydrogen bonding with adenine in RNA. [6] In RNA, uracil forms cyclic

An efficient synthesis of tetramic acid derivatives with extended conjugation from L-Ascorbic Acid

• Biswajit K. Singh,
• Surendra S. Bisht and
• Rama P. Tripathi

Beilstein J. Org. Chem. 2006, 2, No. 24, doi:10.1186/1860-5397-2-24

• natural products of terrestrial and marine origin. [1][2][3][4][5][6] They exhibit a wide range of biological activities including antibiotic, antiviral, antifungal, cytotoxic and enzyme inhibitory activities against bacterial DNA-directed RNA polymerases. [7][8][9] A few of the recently discovered

The first salen- type ligands derived from 3',5'-diamino- 3',5'-dideoxythymidine and -dideoxyxylothymidine and their corresponding copper(II) complexes

• Daniel Koth,
• Michael Gottschaldt,
• Helmar Görls and
• Karolin Pohle

Beilstein J. Org. Chem. 2006, 2, No. 17, doi:10.1186/1860-5397-2-17

• containing three (DNA nucleosides) and four (RNA nucleosides) chiral centers respectively. Though the coordination of metal ions to nucleosides is well known, mostly the nucleobase interacts with the metal centre, and the chiral part of the nucleoside is not involved in the coordination. [1][2] Although a