Methodology for awakening the potential secondary metabolic capacity in actinomycetes

• Shun Saito and
• Midori A. Arai

Beilstein J. Org. Chem. 2024, 20, 753–766, doi:10.3762/bjoc.20.69

• , and the associated undiscovered secondary metabolite biosynthesis genes are called “silent” genes. This review outlines several approaches to further activate the metabolic potential of actinomycetes. Keywords: actinomycete; co-culture; heat shock metabolites (HSMs); secondary metabolites; silent

• environments as a means of ensuring thermotolerance [100][101][102]. As this suggests that HSMs may support thermotolerance, Saito et al. are also currently trying to determine why HSMs are produced. Co-culture It is also important to maintain the culture environment in a state close to that of the natural

• as communication molecules [107][108]. Therefore, various studies have attempted to activate secondary metabolite biosynthetic genes under culture conditions that mimic the natural environment. In particular, the co-culture method, in which multiple microorganisms are cultured simultaneously, has

Chemical and biosynthetic potential of Penicillium shentong XL-F41

• Ran Zou,
• Xin Li,
• Xiaochen Chen,
• Yue-Wei Guo and
• Baofu Xu

Beilstein J. Org. Chem. 2024, 20, 597–606, doi:10.3762/bjoc.20.52

• clusters (BGCs), several methods can be utilized, for instance, epigenetic regulation, co-culture, precursor feeding, heterologous expression, and changing fermentation parameters [11][12][13][14]. In the present study, we focused on a newly identified Penicillium strain, Penicillium shentong XL-F41. To

Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents

• Mingjun Yuan,
• Song Lin Chua,
• Yang Liu,
• Daniela I. Drautz-Moses,
• Joey Kuok Hoong Yam,
• Thet Tun Aung,
• Roger W. Beuerman,
• May Margarette Santillan Salido,
• Stephan C. Schuster,
• Choon-Hong Tan,
• Michael Givskov,
• Liang Yang and
• Thomas E. Nielsen

Beilstein J. Org. Chem. 2018, 14, 3059–3069, doi:10.3762/bjoc.14.284

• macrophages were treated with the T3SS deficient ΔpscJ mutant in DMEM medium. The co-culture was incubated at 37 °C, 5% CO2 for 2 h. The extracellular bacterial cells in DMEM were removed and the infected macrophages were washed 3 times with PBS. Fresh DMEM was added to the infected macrophages and a further

Opportunities and challenges for the sustainable production of structurally complex diterpenoids in recombinant microbial systems

• Katarina Kemper,
• Max Hirte,
• Markus Reinbold,
• Monika Fuchs and
• Thomas Brück

Beilstein J. Org. Chem. 2017, 13, 845–854, doi:10.3762/bjoc.13.85

• taxadiene macrocycle [28]. Engineering one host alone can therefore sometimes be insufficient since there are specific elements of biosynthetic pathways that may have different production capacities in one organism or another. Zhou et al. [94] reported stable co-culture fermentation of specifically

Activation of cryptic metabolite production through gene disruption: Dimethyl furan-2,4-dicarboxylate produced by Streptomyces sahachiroi

• Dinesh Simkhada,
• Huitu Zhang,
• Shogo Mori,
• Howard Williams and
• Coran M. H. Watanabe

Beilstein J. Org. Chem. 2013, 9, 1768–1773, doi:10.3762/bjoc.9.205

• microorganisms communicate (within a single bacterial species or between diverse species) and alter their gene expression through production of certain signaling molecules [3]. In this regard, the culturing of microbial communities or the co-culture of two specific microbial organisms has been successfully