On the road to sustainability – application of metallic nanoparticles obtained by green synthesis in dentistry: a scoping review

• Lorena Pinheiro Vasconcelos Silva,
• Joice Catiane Soares Martins,
• Israel Luís Carvalho Diniz,
• Júlio Abreu Miranda,
• Danilo Rodrigues de Souza,
• Éverton do Nascimento Alencar,
• Moan Jéfter Fernandes Costa and
• Pedro Henrique Sette-de-Souza

Beilstein J. Nanotechnol. 2025, 16, 1851–1862, doi:10.3762/bjnano.16.128

• into scaffolds and regenerative membranes designed to stimulate osteogenesis and promote periodontal tissue regeneration [16][17]. These nanoparticles not only provide antimicrobial protection but also actively modulate cellular behavior, such as proliferation and differentiation, thereby enhancing the

Nanomaterials for biomedical applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Filippo Pierini,
• Seda Kizilel and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 1499–1503, doi:10.3762/bjnano.16.105

• , Istanbul, Turkey 10.3762/bjnano.16.105 Keywords: biomedical applications; drug delivery; nanocarriers; nanomaterials; nanomedicine; nanoparticles; polymeric nanoparticles; tissue regeneration; Medicine has rapidly advanced over the last few decades, and nanotechnology has played a significant role in

• adverse effects and increasing the success rate of the delivery. Since nanomaterials can be tunable, the vast majority of health sectors are investigating their potential in a wide range of applications, such as targeted drug delivery, gene therapy, tissue regeneration, imaging, and diagnostic tools [2

• used in the future for tissue recovery, which was previously thought to be beyond repair [31]. In addition to diagnosis and tissue regeneration, nanomaterials can create new treatment methods and enhance the design and performance of medical implants. An area that is getting a lot of attention is

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• , hybrid systems can overcome the disadvantages of each component of the hybrid system. Hydrogel systems included with liposomes demonstrate exceptional chemical tunability, biocompatibility, and biodegradability. These hybrid platforms offer enormous potential for facilitating tissue regeneration or for

Hydrogels and nanogels: effectiveness in dermal applications

• Jéssica da Cruz Ludwig,
• Diana Fortkamp Grigoletto,
• Daniele Fernanda Renzi,
• Wolf-Rainer Abraham,
• Daniel de Paula and
• Najeh Maissar Khalil

Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90

• recent years, such as tissue regeneration or tumor models in vivo. For example, 3D-printed scaffolds have been employed and shown to be effective in bone regeneration [226] and in promoting the restoration of craniofacial cartilage defects [227]. Also, in in vivo breast cancer models, doxorubicin-loaded

• vehicle [169][170][179]. Hence, these are the basic principles for designing a new topical DDS, and all of them should be addressed to reach the goal, whether it is a nanostructured system or not. Hydrogel dermal applications When obtained from natural polymers, hydrogels are efficient in tissue

• regeneration due to their environment, which allows the modulation of cellular behavior [181][182][183]. The three-dimensional structure, along with cell permeability and mechanical stability, also qualifies hydrogels as useful drug carriers. Hydrogels have been used for dermal applications due to their

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• potential to accelerate bone tissue regeneration and promote bone healing. These findings offer a promising avenue for developing new therapies for bone-related injuries and conditions requiring significant bone remodeling. Keywords: bone healing; hexagonal boron nitrides; human osteoblasts; nanomaterials

• biocompatible wireless tissue stimulation applications but also envision a future where these materials could revolutionize noninvasive therapeutic strategies. The biocompatibility and effectiveness of hBNs make them a promising candidate for future therapeutic applications aimed at enhancing tissue

• regeneration and bone healing, potentially ushering in a new era of medical advancements. PRFM amplitude and phase images of hBNs and BaTiO3. Viability of HOb cells treated with hBNs, hBNs+US, BaTiO3, and BaTiO3+US for (a) 24 h and (b) 48 h. Statistically significant change compared to the control group were

Colloidal few layered graphene–tannic acid preserves the biocompatibility of periodontal ligament cells

• Teissir Ben Ammar,
• Naji Kharouf,
• Dominique Vautier,
• Housseinou Ba,
• Nivedita Sudheer,
• Philippe Lavalle and
• Vincent Ball

Beilstein J. Nanotechnol. 2025, 16, 664–677, doi:10.3762/bjnano.16.51

• transformative approach to enhance their efficacy [2]. Actually, incorporating nanomaterials into dental biomaterials has already offered advantages like enhanced tissue regeneration, increased mechanical strength of composites, and improved sealing of filler materials [3]. Graphene-based materials (GBMs) stand

A formulation containing Cymbopogon flexuosus essential oil: improvement of biochemical parameters and oxidative stress in diabetic rats

• Ailton Santos Sena-Júnior,
• Cleverton Nascimento Santana Andrade,
• Pedro Henrique Macedo Moura,
• Jocsã Hémany Cândido dos Santos,
• Cauãn Torres Trancoso,
• Eloia Emanuelly Dias Silva,
• Deise Maria Rego Rodrigues Silva,
• Ênio Pereira Telles,
• Luiz André Santos Silva,
• Isabella Lima Dantas Teles,
• Sara Fernanda Mota de Almeida,
• Daniel Alves de Souza,
• Jileno Ferreira Santos,
• Felipe José Aidar Martins,
• Ana Mara de Oliveira e Silva,
• Sandra Lauton-Santos,
• Guilherme Rodolfo Souza de Araujo,
• Cristiane Bani Correa,
• Rogéria De Souza Nunes,
• Lysandro Pinto Borges and
• Ana Amélia Moreira Lira

Beilstein J. Nanotechnol. 2025, 16, 617–636, doi:10.3762/bjnano.16.48

• indicate that, despite the significant damage observed in the MET group, there are signs of tissue regeneration, showing a dynamic balance between inflammatory aggression and the liver’s reparative response. Histopathological analysis of the CONTROL group (Figure 13) revealed less inflammation and vascular

Polyurethane/silk fibroin-based electrospun membranes for wound healing and skin substitute applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Muhammad Waqas,
• Ahsan Nazir and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 591–612, doi:10.3762/bjnano.16.46

• transport and gas exchange, which supports tissue regeneration and cell proliferation [22]. Furthermore, they have mechanical qualities that are similar to those of natural tissues, promoting and protecting the healing process [23]. Review Wound healing and skin regeneration The skin is a vital, protective

• more effective wound-healing substitutes [50]. Recent advancements in tissue regeneration have further led to the development of innovative wound dressings such as hydrogels and electrospun scaffolds [51]. Electrospinning is an extremely flexible technique that can convert liquids, suspensions, or

• functional similarities as both serve as supportive framework. Electrospun membranes are specifically designed to replicate the fibrous architecture and functional properties of the ECM, thereby promoting cellular activity and facilitating tissue regeneration in the same way the natural matrix does within

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

Nanocarriers and macrophage interaction: from a potential hurdle to an alternative therapeutic strategy

• Naths Grazia Sukubo,
• Paolo Bigini and
• Annalisa Morelli

Beilstein J. Nanotechnol. 2025, 16, 97–118, doi:10.3762/bjnano.16.10

• inflammation through the secretion of IL-10 and low levels of TNF-α. M2c macrophages, stimulated by IL-10 and glucocorticoids, resolve inflammation and promote tissue regeneration. Last, M2d macrophages, known as tumor-associated macrophages (TAMs), are activated by adenosine and IL-6 and characterized by

• factors such as PPARγ has shown the potential to promote M2 polarization, thereby supporting tissue regeneration and reducing chronic inflammation. These systems demonstrate the versatility of mRNA-based therapies in addressing various pathological conditions. However, translating RNA-based therapeutics

Natural nanofibers embedded in the seed mucilage envelope: composite hydrogels with specific adhesive and frictional properties

• Agnieszka Kreitschitz and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2024, 15, 1603–1618, doi:10.3762/bjnano.15.126

• tasteless [11][21]. Its chemical composition and special physical properties allow many applications of mucilage, for example, as thickening and structuring (gel-forming) agent, emulsifier or stabiliser for food products, scaffold for tissue regeneration, additive in formation of medicinal tablets, and for

Electrospun nanofibers: building blocks for the repair of bone tissue

• Tuğrul Mert Serim,
• Gülin Amasya,
• Tuğba Eren-Böncü,
• Ceyda Tuba Şengel-Türk and
• Ayşe Nurten Özdemir

Beilstein J. Nanotechnol. 2024, 15, 941–953, doi:10.3762/bjnano.15.77

• mixed polymers, and the formation of highly porous and continuous fibers are the remarkable features of this method. The importance of nanofiber-based scaffolds in bone tissue regeneration is increasing because of suitable pore size, high porosity, osteoinduction, induction of bone growth with

• , antibiotics, anticancer agents, proteins, DNA, RNA, and growth factors for tissue regeneration [6][7][8]. In addition, nanofibers as drug delivery systems provide rapid or delayed and controlled release of pharmaceuticals. Apart from being implantable drug delivery systems, nanofiber scaffolds can contribute

• -based nanofiber scaffolds for tissue regeneration (Stellenbosch Nanofiber Company, South Africa) [45]. ReBOSSIS consists of β-tricalcium phosphate, PLGA, and silicon-doped calcium carbonate to support bone formation. ReBOSSIS electrospun fibers are distinguished from other market products by a cotton

Electrospun polysuccinimide scaffolds containing different salts as potential wound dressing material

• Veronika Pálos,
• Krisztina S. Nagy,
• Rita Pázmány,
• Krisztina Juriga-Tóth,
• Bálint Budavári,
• Judit Domokos,
• Dóra Szabó,
• Ákos Zsembery and
• Angela Jedlovszky-Hajdu

Beilstein J. Nanotechnol. 2024, 15, 781–796, doi:10.3762/bjnano.15.65

• strategies to fight bacterial pathogens with similar effects to antibiotics and silver is essential. In our study, inorganic salts, namely Zn(O2CCH3)2 and Sr(NO3)2, were added to the polymer fibers. These salts possess antibacterial properties and stimulate cell proliferation as well as tissue regeneration

Biomimetic chitosan with biocomposite nanomaterials for bone tissue repair and regeneration

• Se-Kwon Kim,
• Sesha Subramanian Murugan,
• Pandurang Appana Dalavi,
• Sebanti Gupta,
• Sukumaran Anil,
• Gi Hun Seong and
• Jayachandran Venkatesan

Beilstein J. Nanotechnol. 2022, 13, 1051–1067, doi:10.3762/bjnano.13.92

• and physically comparable microenvironment to that of the natural extracellular matrix, containing healing and stimulating components necessary for bone repair, making them a potential option for bone tissue regeneration. As carbon nanotubes are combined with natural polymers, such as chitosan and

• , polyhydroxyethylmethacrylate, and polyethylene glycol were used in composite preparation for bone tissue engineering applications [10]. To create a fibrous scaffold for bone tissue regeneration, zein and chitosan were combined with polyurethane and functionalized multiwalled carbon nanotubes. The developed scaffolds have

• approach has been used to create a graphene oxide with chitosan and hydroxyapatite nanocomposite film for a possible use in bone tissue regeneration. As per study results, the combined chitosan and hydroxyapatite nanocomposite film provides an excellent substrate for the growth of mouse mesenchymal stem

Bioselectivity of silk protein-based materials and their bio-inspired applications

• Hendrik Bargel,
• Vanessa T. Trossmann,
• Christoph Sommer and
• Thomas Scheibel

Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81

• biomedicine and tissue engineering, since they exhibit promising chemical and physical properties, such as bioactivity, structural integrity, and cell stimulation [29][30]. Biomimetic materials modulating specific cellular responses and tissue regeneration have been developed by adjusting and modifying

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• for cartilage tissue regeneration because it can respond to external magnetic fields and specifically relocate to the defective cartilage to resurface the injured site [62]. Initial OA is a slow, complex process characterized by the inflammation and subsequent production of cartilage degradation

• traditionally incorporated in scaffolds to provide proper cell–matrix interactions for tissue regeneration [149]. Among these, cell adhesion motifs including RGD peptide and fibronectin fragments at the nanoscale are widely used to enhance the cell motility and adhesion functionality of hydrogels [178]. For

Wet-spinning of magneto-responsive helical chitosan microfibers

• Dorothea Brüggemann,
• Johanna Michel,
• Naiana Suter,
• Matheus Grande de Aguiar and
• Michael Maas

Beilstein J. Nanotechnol. 2020, 11, 991–999, doi:10.3762/bjnano.11.83

• immunogenicity [17] and, therefore, has become highly used in tissue regeneration [18][19]. Chitosan fibers are particularly well-suited for tissue engineering due to their highly porous scaffold architecture [20]. Using electrospinning, chitosan fibers can be produced with a diameter ranging from several tens

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• for the tissue regeneration therapy in biomedicine. Keywords: atomic force acoustic microscopy (AFAM); cell growth; nanopattern; stiffness; SU-8 photoresist; topography; Introduction The interactions of cells with extracellular matrices (ECMs) play important roles in regenerative medicine and tissue

Nanocellulose: Recent advances and its prospects in environmental remediation

• Katrina Pui Yee Shak,
• Yean Ling Pang and
• Shee Keat Mah

Beilstein J. Nanotechnol. 2018, 9, 2479–2498, doi:10.3762/bjnano.9.232

Engineering of oriented carbon nanotubes in composite materials

• Razieh Beigmoradi,
• Abdolreza Samimi and
• Davod Mohebbi-Kalhori

Beilstein J. Nanotechnol. 2018, 9, 415–435, doi:10.3762/bjnano.9.41

• oxides for metal-free catalysis [15] or in synergy with metal oxides [16][17], especially for sustainable energy applications [18][19]. Because of their electronic properties, CNT composites offer unmatched opportunities for conductive tissue regeneration [20], particularly if alignment, and thus 3D

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• a strongly demanded feature in every kind of templates and scaffolds for tissue regeneration [21][22]. A biologically derived or biocompatible synthetic LC material can provide close resemblance to those in native tissues, by forming self-assembled 3D nanoarchitectures. Nevertheless, this aspect has

• for applying LC nanoarchitectures to tissue regeneration purposes is discussed. Liquid crystalline phases of biological polymers and colloids in vitro Geometry in biological LCs Lyotropic LC phases are observed in the aqueous dispersions of various types of biological rod-like building blocks, such as

• [36] and drug delivery systems [37]. For tissue regeneration, the mostly studied biomaterials are collagen and chitin, which are, respectively, protein-based and glucose-based biopolymers [38][39]. When denatured, collagen and chitin can be transformed into gelatin and chitosan, respectively, which

Surface functionalization of 3D-printed plastics via initiated chemical vapor deposition

• Christine Cheng and
• Malancha Gupta

Beilstein J. Nanotechnol. 2017, 8, 1629–1636, doi:10.3762/bjnano.8.162

• hydrophobic surface properties and do not promote cellular differentiation [3][18]. Surface modification of printed scaffolds can allow for the tuning of surface functionalization to promote vascularization and tissue regeneration while maintaining control over the mechanical robustness of the bulk structure

Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots

• Maria C. Cringoli,
• Slavko Kralj,
• Marina Kurbasic,
• Massimo Urban and
• Silvia Marchesan

Beilstein J. Nanotechnol. 2017, 8, 1553–1562, doi:10.3762/bjnano.8.157

• long term, a supramolecular hydrogel composed of a peptide and luminescent nanodots could be valuable for tissue regeneration based on a bioactive scaffold that can be also visualized in vivo by fluorescence microscopy. Alternatively, other potential applications could be developed in the future for

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

Fabrication of hybrid nanocomposite scaffolds by incorporating ligand-free hydroxyapatite nanoparticles into biodegradable polymer scaffolds and release studies

• Balazs Farkas,
• Marina Rodio,
• Ilaria Romano,
• Alberto Diaspro,
• Romuald Intartaglia and
• Szabolcs Beke

Beilstein J. Nanotechnol. 2015, 6, 2217–2223, doi:10.3762/bjnano.6.227

• similarity to the mineral constituent of human bones, are bioactive and can be fairly easily bioconjugated [1]. HA NPs can enhance cell proliferation in bone tissue regeneration [2]. Tissue engineering is an interdisciplinary field that combines the principles of life sciences and engineering to improve

• tissue growth and functions. Whenever the need arises for a certain type of scaffold to be produced, all these fields have to be utilized together to get an appropriate solution. HA is an essential ingredient of normal bone and teeth and is widely used for bone tissue regeneration. Given the high degree