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Search for "protein corona" in Full Text gives 61 result(s) in Beilstein Journal of Nanotechnology.
Effect of different silica coatings on the toxicity of upconversion nanoparticles on RAW 264.7 macrophage cells
Beilstein J. Nanotechnol. 2021, 12, 35–48, doi:10.3762/bjnano.12.3
- -average values of the samples after redispersion in DMEM were lower than in water, except for the samples UC@thin_NH2, UC@thick_RBITC_NH2, and SiO2@RBITC_NH2. The lower Z-average values of these samples may indicate an increased stabilization by a protein corona [52][53][54][55][56]. However, the high
- aggregation of silica nanoparticles that occurred after redispersion in buffered solution and in physiological medium [54]. They reported that various proteins in a medium containing FBS were adsorbed onto the surface of bare SiO2 and amine-functionalized SiO2 nanoparticles, forming a protein corona with a
- new surface charge, which depended on the type of proteins that built the corona. The adsorbed protein corona, consisting of the proteins present in FBS, could increase or reduce the stability of the particles and, consequently, their hydrodynamic diameter [53][54][55][56][57]. The non-functionalized
Key for crossing the BBB with nanoparticles: the rational design
Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72
- protein corona forming around them, by investigating the particle–cell interactions or by looking for biomimetic solutions. Blood–brain barrier anatomy. Inspired by [5]. Brain delivery routes. A) Local delivery. Drugs can reach the brain by direct injection through the meninges. B) Intranasal delivery
Identification of physicochemical properties that modulate nanoparticle aggregation in blood
Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44
- purpose of investigating the role of surface curvature and chemistry on platelet aggregation, activation and adhesion. Substantial differences were found in the composition of the protein corona depending on the chemical nature of the nanoparticles, while the surface curvature was found to play a minor
- –protein interaction may lead to bridging among particles, thus promoting agglomeration [23]. In the present study, a set of six silica and carbon NPs of known size and morphology was used to evaluate the effect of the size and surface properties on the protein corona composition, platelet activation and
- polystyrene cuvette, at 25 °C. PBS 0.01 M, pH 7.4, Sigma-Aldrich, was used as the diluent in the case of the evaluation of the size after the protein corona formation. Nanoparticle tracking analysis (NTA) An analysis of the size distribution and concentration of CNPs and SNPs were performed by NTA using a
Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine
Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25
- to predict the evolution of their protein corona [60][61][198]. Other studies are trying to understand not only whether certain biomolecules are present on the nanoparticle surface, but also their orientation, which might influence their recognition by cell receptors [17][199]. In order to take into
Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy
Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20
- . Specifically, the recently proposed protein corona sensor arrays in which the composition of protein corona reflects the presence of a given cancer enabled new venues in detecting diseases directly from a blood sample [140]. Moreover, the simultaneous detection of genetic mutations and disease-specific
- ]. Several limitations need to be addressed as well. One of the known issues in colloidal biosensing is the spontaneous formation of a protein corona on the surface of the particles in physiological media [146], inhibiting the interaction of the biomarkers with the colloid, thereby altering the sensitivity
Rational design of block copolymer self-assemblies in photodynamic therapy
Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15
Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells
Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246
- polydisperse proteins of different sizes [27]. Indeed, the surface properties of various nanoparticles have been shown to change dramatically in the presence of plasma or serum [28] with the establishment of an adsorbed protein corona around the nanoparticle. It is now widely accepted that the particle protein
- corona presents a new biomolecular interface that underpins the dynamic interactions of nanosystems and their biological targets. The presentation of a high affinity GRPR antagonist peptide on the liposomal surface is expected to maintain liposomal cell-binding affinity by virtue of its high affinity for
Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting
Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181
- proteins, resulting in the formation of protein corona [48][49]. To minimize the adverse effects of the presence of the protein corona in vivo, the surface coating using PEG can endow the NPs with so-called “stealth” properties to reduce the adsorption of high molecular weight proteins, allowing them to
Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles
Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101
- /bjnano.10.101 Abstract Background: When nanoparticles (NPs) are applied into a biological fluid, such as blood, proteins bind rapidly to their surface forming a so-called “protein corona”. These proteins are strongly attached to the NP surface and confers them a new biological identity that is crucial
- for the biological response in terms of body biodistribution, cellular uptake, and toxicity. The corona is dynamic in nature and it is well known that the composition varies in dependence of the physicochemical properties of the NPs. In the present study we investigated the protein corona that forms
- assay, zeta potential measurements, and liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS). Additionally, the time-dependent cell interaction of PLGA NPs in the absence or presence of a preformed protein corona was assessed by in vitro incubation experiments with the human liver cancer
Engineering of oriented carbon nanotubes in composite materials
Beilstein J. Nanotechnol. 2018, 9, 415–435, doi:10.3762/bjnano.9.41
- the CNT diameter and length are critical parameters in protein corona formation and biocompatibility [11][12]. In another research investigating the effect of aligning CNTs in composite material structures, a remarkable improvement in the electrical properties of CNT composites was observed, as
Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications
Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174
- have a dramatic effect on the formation of the surface protein corona in the bloodstream that affects CoFe2O4@Met–Au NPs passive targeting and uptake into tumor cells. The elaborated functionalization of magnetic NPs with gold QDs represents a promising multi-task platform for linking magnetic NPs with
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130
- amounts of salts, amino acids and proteins. In aqueous media, electrostatic repulsion contributes to particle stabilisation. In presence of salts at neutral pH values, this type of stabilisation might be ineffective. In contrast, the formation of a protein corona at the interface between particle surface
Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets
Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125
- between Qdots and functional antibodies, the overall surface charge of Qdot-Ab conjugates, and whether there is a presence of a protein corona. The labelling ratio of Qdots to antibodies present within commercially available Qdot-Ab conjugates has been previously evaluated, where the non-specific
- protein adsorption and thus the formation of a protein corona [38]. Qdots with a zwitterionic surface have a zeta potential of near zero, are resistant to non-specific binding onto cells, and have a high colloidal stability [39]. Commercially available Qdot-Abs evaluated in this report were used in fixed
Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery
Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123
- , which could be a desirable event in targeted tumour therapy. The optimal uptake conditions for NPs could depend on the particle size, surface modifications, protein corona, and recipient cell line. Previous studies have suggested the incubation of NIH3T3 mouse fibroblasts with 16 nM QDs for 6 h as the
- varies depending on the cell and particle type. One of the factors affecting uptake is the protein corona that forms around NPs in serum-containing medium. Protein aggregates decrease gold NP uptake depending on size and cell type [41]. In the present study, we analysed the QD uptake pathways under both
- QDs possessing a protein corona are differently recognized by NIH3T3 cells and internalized by different pathways [23], consistent with the data from the present study. Interestingly, MSCs showed more effective internalization of QDs under serum-free conditions, as the protein corona interferes with
Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40
- dispersion in biological media containing proteins (like RPMI/10% FCS) is much better due to the formation of a protein corona [48][49]. The concentration of the labelled proteins was determined by UV–vis spectroscopy (Table 2). Table 3 gives the quantification data of the functionalized nanoparticles, where
- also influence their uptake [64][65][66][67]. Krais et al. have shown that serum proteins were necessary for cancer cells to take up folic acid-conjugated iron oxide nanoparticles [68]. The nature of the protein corona on the protein-loaded calcium phosphate nanoparticles after immersion in cell
On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121
- dependent on particle size and the serum content in the culture medium [11][12]. In serum-free conditions the uptake of SiNPs has been determined to be much higher than in the presence of fetal calf serum (FCS), which is attributed to the fact that SiNPs build up a protein corona and tend to agglomerate in
Surface coating affects behavior of metallic nanoparticles in a biological environment
Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23
- may serve as desirable barriers preventing NPs from agglomeration in biomedical applications [18]. Moreover, when NPs enter a biological fluid, electrostatic, dispersive, and covalent interactions cause proteins to adsorb on NP surfaces, leading to the formation of a dynamic protein corona [30][36][37
- uptake and toxicity of metallic NPs [41][42], whereas differences in surface coatings influence cytotoxicity and surface charge [43]. However, it is still unclear how different surface coatings affect the interaction of NPs with biological environments and the formation of the protein corona. Because
- evaluation of metallic NPs in biological environments. Effect of albumin on the dispersibility of AgNPs and SPIONs in biological media When suspended in biological fluids, NPs rapidly interact with proteins that form a dynamical layer all over the NP surface, known as a protein corona (PC) [36][37][39
The eNanoMapper database for nanomaterial safety information
Beilstein J. Nanotechnol. 2015, 6, 1609–1634, doi:10.3762/bjnano.6.165
- templates (e.g., Protein Corona CSV files or ModNanoTox Excel files), and custom formats, provided by partners (e.g., the NanoWiki RDF dump [27]). ISA-Tab [15] files are converted by compressing the chain of protocols into a single entry, yet retaining all the protocol parameters and recording the material
- allowing parsing of the entire ModNanoTox complex organisation into the internal eNanoMapper data model. Protein Corona The demonstration data set, extracted from [28], focuses on the biological identity of ENMs. The authors used the composition of the protein corona “fingerprint” to predict the cell
- . Among the factors in play in protein corona, biological interaction was chosen to be represented by cell association because of its relevance to biodistribution, inflammatory response potential, and in vivo toxicity. The eNanoMapper prototype described in this paper is able to capture this protein
Protein corona – from molecular adsorption to physiological complexity
Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88
- Hospital of Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany 10.3762/bjnano.6.88 Abstract In biological environments, nanoparticles are enshrouded by a layer of biomolecules, predominantly proteins, mediating its subsequent interactions with cells. Detecting this protein corona, understanding its
- formation with regards to nanoparticle (NP) and protein properties, and elucidating its biological implications were central aims of bio-related nano-research throughout the past years. Here, we discuss the mechanistic parameters that are involved in the protein corona formation and the consequences of this
- involvement of Coulomb-type interactions between NPs and charged patches on the protein surface. Moreover, we discuss novel aspects related to the complexity of the protein corona forming under physiological conditions in full serum. Specifically, we address the relation between particle size and corona
Biological responses to nanoscale particles
Beilstein J. Nanotechnol. 2015, 6, 380–382, doi:10.3762/bjnano.6.37
- , their surface-active components (i.e., proteins) will adhere to the nanoparticles and form a so-called “protein corona”. As a result, biological systems never come into contact with bare nanoparticles, but rather with their protein-coated analogues. Since this discovery, a large variety of experimental
- preferred as a function of shape, size, and most importantly, surface functionalization and protein corona, could thus far not be established. The SPP1313 research network resulted from an open national call after an intensive evaluation of proposals by an international group of experts. It operated for a
Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques
Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25
- their toxicity is of prime interest in the safety evaluation of NP. Importantly, for a precise understanding of the biological and toxicological effects of complex NP it is important to understand which part of the NP induces the observed effects, e.g., the inorganic core, the ligand shell, the protein
- corona or even the drug or label inside or associated with the particle. Therefore, more sophisticated imaging methods are needed that allow one to distinguish different parts of a NP within tissues or cells. Furthermore, the expertise of specially trained toxicological pathologists is essential in any
The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice
Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11
- to play important roles in endocytosing processes and are known to be among the most prominent cell types which take up protein-corona-covered nanoparticles [22][40]. The confocal images of the cryosections exhibit the cell distribution within the liver and confirm this hypothesis. Internalized Qdots
The fate of a designed protein corona on nanoparticles in vitro and in vivo
Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5
- back into the plasma and into tissue. This study confirms that adsorbed transferrin from a preformed protein corona is efficiently taken up by cells. It is also highlighted that a radiolabelling technique described in this study may be of value to investigate the role of protein corona formation in
- vivo for the respective nanoparticle uptake. Keywords: albumin; 59Fe; 125I; organ uptake; protein corona; SPIOs; transferrin; Introduction Nanoparticles (NPs) have unique capabilities to interact with cells and organs which mark them as attractive working material in nanobioscience and nanomedicine
- with biological matrices such as the blood, are immediately coated by a layer of proteins, resulting in a so called „protein corona“ [8][9][10][11][12][13][14]. The protein corona modifies and shields the surface of the xenobiotic particles and may subsequently influence or even determine their
Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects
Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248
- machinery. An interesting aspect in this process is that, in the biological milieu, the NPs typically adsorb dissolved biomolecules, so that they are enshrouded by a so-called ‘protein corona’ [6][7][8]. NPs interact with cells via this layer of biomolecules, at least during the initial encounter, so that
- [24][25][26][27]. Because of the inevitable protein corona formation in any biological environment, one has also to be aware that experiments on cultured cells may yield results different from in vivo studies. Over the past few years, we have used spinning disk confocal fluorescence microscopy to
- cells and the bare NP surfaces rather than NPs carrying a protein corona of unknown composition. By means of inhibitory drugs that specifically interfere with the one or the other endocytosis pathway, the endocytosis pathways involved in the uptake of small and large NPs have been revealed. Finally
Inorganic Janus particles for biomedical applications
Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244
- activity, the magnetic properties, as well as their biocompatibility and interaction with human blood serum. Keywords: bioimaging (CT; MRI; Multi-photon); hetero-nanoparticles; Janus particles; protein corona; synthesis; Introduction In the recent years, there has been an increasing interest in design
- nanoparticles, while the composition of the protein corona is responsible for the fate of the nanoparticles in the organism. Up to now, the formation and the composition of the protein corona is far from being completely understood, but the importance is obvious for any further application of nanoparticles in
- theranostics [113]. The analysis of the protein corona of nanoparticles shows that the binding profiles do not reflect the relative protein concentrations of the plasma. Recently, Tenzer et al. showed that there is no direct correlation of the surface charge and the isoelectric point of proteins enriched in
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