Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

Biocompatible organic–inorganic hybrid materials based on nucleobases and titanium developed by molecular layer deposition

• Leva Momtazi,
• Henrik H. Sønsteby and
• Ola Nilsen

Beilstein J. Nanotechnol. 2019, 10, 399–411, doi:10.3762/bjnano.10.39

• films were relatively hydrophilic (Ti-thymine (deposited at 225 °C) = 19 ± 2°, Ti-uracil (deposited at 225 °C) = 19 ± 1°, and Ti-adenine (deposited at 250 °C) = 45 ± 3°), Figure 6. This hydrophilicity is highly suitable for cell growth purposes. A similar hydrophilic nature was observed for Ti-glycine

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• vectorization for enhancing the cell growth inhibition. In these cases, the goal is to drive the payload to the nucleus after selective internalization in the cytoplasm. Nuclear delivery with the HIV trans-activator of transcription (TAT) peptoid in combination with the vasculature and tumor cell membrane

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• ; the particle size associated with the controlled reaction time and the annealing of the material at 375 °C slightly negatively influenced the interaction with cells, but no toxic effect was observed. Most of the materials induced cell growth at low concentration levels. The overdose effect is best

• seen at 300 µg/mL. For A549 cells, no toxicity was noted in the tested concentration range and there was no strong cell-growth inhibition. BEAS-2B cells were the least affected by the presence of HAp nanoparticles, and no strong growth inhibitions were noted in this case as well. A toxic effect on the

• ][64][65]. Cell-growth inhibition could be related to the presence of a slight excess of calcium ions inside the cytoplasm, which disrupts the intracellular calcium homeostasis and inhibits cells transcriptional and translational processes [66][67], slowing down proliferation and cell growth. Calcium

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• streptomycin at 37 °C in humidified atmosphere containing 5% CO2, while hMSCs were cultured in mesenchymal stem cell growth medium (MSCGM BulletKitTM; Lonza, Italy). All other cells were cultured in RPMI medium supplemented with 10% fetal calf serum, streptomycin (50 µg/mL), and penicillin (50 units/mL) at 37

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• (NIH 3T3). We observed an IC50 value of 4.5 µg/mL and 6.5 µg/mL in A549 and HCT-15 cell lines, respectively (Figure 4B,C). Furthermore, upon administration of 5-FU at a concentration of 100 µg/mL, a 67.02% and 81.5% reduction in cell growth in HCT-15 and A549 cell lines was observed. CaP@5-FU NPs

• population corroborates the previous results of the ROS induction assay, AO/EB observations, and SEM micrographs. The results infer the detrimental effect of 5-FU-induced toxicity on cell growth and multiplication [50]. The change in the mitochondrial membrane potential over the course of a 24 h treatment

Fabrication of photothermally active poly(vinyl alcohol) films with gold nanostars for antibacterial applications

• Mykola Borzenkov,
• Maria Moros,
• Claudia Tortiglione,
• Serena Bertoldi,
• Nicola Contessi,
• Silvia Faré,
• Angelo Taglietti,
• Agnese D’Agostino,
• Piersandro Pallavicini,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2018, 9, 2040–2048, doi:10.3762/bjnano.9.193

• explored in numerous studies to eradicate cancer cells, controlled drug delivery and enhancement of cell growth [1][2][3][4][5]. Among the many types of such nanoparticles, gold nanostars (GNSs) (having a well-tunable LSPR position in the biotransparent NIR window (750–1300 nm) and ease of surface

• irradiation with a 1064 nm laser, as registered by a thermocamera (laser intensity 4 W/cm2). (b) After 5 minutes of laser irradiation (or simply exposure to the air for the control samples) the films were transferred to LB broth and left at 37 °C for cell growth. The number of bacteria per mL was calculated

Synthesis of carbon nanowalls from a single-source metal-organic precursor

• André Giese,
• Sebastian Schipporeit,
• Volker Buck and
• Nicolas Wöhrl

Beilstein J. Nanotechnol. 2018, 9, 1895–1905, doi:10.3762/bjnano.9.181

• superhydrophilic properties, which has a significant effect on cell growth, making CNWs an interesting material for biotechnology (bio-sensors) and medical technology (implants, diagnostics) [13][17]. The growth of CNWs is often referred to as a three-stage process that was originally proposed by Kondo and co

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• show any significant toxicity in either cancerous or non-cancerous cells, implying that the tumour cell growth inhibition properties observed in the spice-derived C-dots can be attributed to the starting material employed for their fabrication. These results evidence that functional groups in the

• significant effect on cell viability neither in LN-229 nor in HK-2 cells (Figure 8) suggests that the inhibition effect on the cell growth of LN-229 cells can be attributed to the nature of the spice-based C-dots, indeed depending on the starting material employed for the C-dots synthesis. Considering the

• reduction in cell viability was observed for LN-229 cells after 24 h of exposure to increasing concentrations of each C-dot type. In fact, results obtained showed that 2 mg/mL of cinnamon, red chilli, turmeric and black pepper C-dots yielded cancer cell growth inhibition efficiencies of 35, 50, 50 and 75

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• [14][15][16]. Moreover, in vitro 3D cultures could fill the gap between 2D in vitro testing and in vivo animal models [17][18]. Spheroid-based 3D models are widely used to test cancer cell growth/proliferation, invasion, angiogenesis, and immune interactions and have been employed in drug screening

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• organizations in living tissues [85]. For instance, Giraud–Guille and colleagues investigated hierarchically ordered surfaces of dense collagen in the cholesteric phase for the use of tissue regeneration templates for aligned cell growth with controlled functions [86]. The collagen fibrils, which are animal

• induced 2D and 3D aligned cell growth in response to the lamellar molecular structure of LCE matrices, with tunable porosity and crosslinking density [99][100][101]. In addition, the 3D anisotropic cell scaffolds can be fabricated by using nematic LCE microspheres as building blocks by tuning elastic

• production of physiologically functional tissue replicas of nerves by controlling 3D cell alignment and growth direction. When cardiomyocytes are embedded in the anisotropic gel scaffolds, the guided cell growth parallel to the major axis of the bundle structure enables the long-range propagation of action

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• ). It is known that progression of CIN from mild dysplasia to invasive cancer is accompanied by the increase in level of epidermal growth factor receptor (EGFR). The overexpression of EGFR has been correlated to uncontrolled cell growth and inhibition of cell apoptosis. Hence, EGFR can be used as a

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• : Human breast cancer cell lines MDA-MB-231 and MCF-7 were obtained from the American Type Culture Collection (ATCC HTB-26™; ATCC HTB-22™). MDA-MB-231 and MCF-7 cells were cultured in cell growth medium (DMEM, Gibco, US), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, US), 100 U/mL

• wells were left without upconverting particles to serve as the control group. After 24 h of treatment, the cell growth medium with nanoparticles was aspirated and cells were washed with DPBS (pH 7.0) three times. To prepare an XTT solution, 0.1 mL activation solution (N-methyl dibenzopyrazine methyl

Laser processing of thin-film multilayer structures: comparison between a 3D thermal model and experimental results

• Babak B. Naghshine and
• Amirkianoosh Kiani

Beilstein J. Nanotechnol. 2017, 8, 1749–1759, doi:10.3762/bjnano.8.176

• can have many potential applications including patterning the cell growth for biomedical applications and controlling the grain size in fabrication of polycrystalline silicon (poly-Si) thin-film transistors (TFTs). Keywords: 3D transient modelling; heat transfer; laser materials processing

• shape of the heat affected zone and ablated area can be significantly altered by applying a thin film on the surface. As mentioned earlier these are the areas that become less and more biocompatible respectively. As a result, controlling the size of these areas makes the concept of patterning the cell

• growth by laser processing even more attractive. Conclusion The model introduced in this paper is able to precisely predict the ablation depth and temperature field of the thin-film coated samples processed by a single nanosecond pulse. Silicon substrates coated with gold and aluminium thin films and

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• Republic 10.3762/bjnano.8.165 Abstract Diamond nanoparticles, known as nanodiamonds (NDs), possess several medically significant properties. Having a tailorable and easily accessible surface gives them great potential for use in sensing and imaging applications and as a component of cell growth scaffolds

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• approximately a third of the chemotherapeutic concentration when carbon nanomaterials were used in combination (Table 2). Effect on short- and long-term cellular proliferation Next, the influence of the single and combinatory treatments on cell growth was evaluated. Similar to the previous study [27], CNFs and

• expected, DTX (2.5 ng/mL) and MMC (0.5 µg/mL) alone promoted a significant inhibition by about 30% and 75%, respectively, of both short- and long-term proliferation (Figure 2). This inhibitory effect on cell growth was further enhanced when the chemotherapeutics were administered in combination with CNFs

• viability and apoptosis following treatment. An enhanced inhibition of tumor cell growth mediated by DTX–CNT complexes could also be observed in in vivo animal models for various tumor entities [18][22]. These studies suggested that DTX–CNT complexes possess a prolonged half-life and higher accumulation

Silicon microgrooves for contact guidance of human aortic endothelial cells

• Sara Fernández-Castillejo,
• Pilar Formentín,
• Úrsula Catalán,
• Josep Pallarès,
• Lluís F. Marsal and
• Rosa Solà

Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72

• aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour. Results: The influence of groove-shaped substrates on

Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring

• Niina Halonen,
• Joni Kilpijärvi,
• Maciej Sobocinski,
• Timir Datta-Chaudhuri,
• Antti Hassinen,
• Someshekar B. Prakash,
• Peter Möller,
• Pamela Abshire,
• Sakari Kellokumpu and
• Anita Lloyd Spetz

Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179

• Eitel reported a LTCC material as a substrate for biosensors that is regarded as biocompatible [22]. Also, from our experience, cell growth, at least over 24 h, seems to be fully compatible with the LTCC material [23]. We suggest that the previous statement about LTCC material being non-biocompatible

• [23]. Human lung epithelial cells (BEAS2B) were cultivated on the chip to verify the biocompatibility of the package material. Responses were obtained on dry chips, chips covered with only cell growth medium (DMEM), and chips covered with a solution of cells in DMEM, demonstrating the robustness and

• baseline to be recorded. A sensor chip with no fluid in the well was placed inside the incubator and data was recorded for 12 min. Then 300 µL of cell growth medium (DMEM) was added into the well, and data was recorded for 50 min with data points taken every 2 min. The maximum rate of the system was 60

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

• Huijuan Zhang,
• Fuqiang Wu,
• Yazhen Li,
• Xiping Yang,
• Jiamei Huang,
• Tingting Lv,
• Yingying Zhang,
• Jianzhong Chen,
• Haijun Chen,
• Yu Gao,
• Guannan Liu and
• Lee Jia

Beilstein J. Nanotechnol. 2016, 7, 1861–1870, doi:10.3762/bjnano.7.178

• hepatocellular carcinoma) cells (Figure 7). CNs demonstrated no obvious toxicity to the four cancer cells under all the tested concentrations, indicating that Cs could be used as a safe drug carrier. However, MCNs could inhibit cell growth in a dose-dependant manner. In this experiment, we found that MIF had no

Viability and proliferation of endothelial cells upon exposure to GaN nanoparticles

• Tudor Braniste,
• Ion Tiginyanu,
• Tibor Horvath,
• Simion Raevschi,
• Serghei Cebotari,
• Marco Lux,
• Axel Haverich and
• Andres Hilfiker

Beilstein J. Nanotechnol. 2016, 7, 1330–1337, doi:10.3762/bjnano.7.124

• toxic Zn from the nanostructures is unlikely. Firstly, the effect of an increasing concentration of free GaN nanoparticles on porcine aortic endothelial cell growth was determined (Figure 2). As a positive control for toxicity, the cells were also exposed to corresponding concentrations of free ZnO

Reasons and remedies for the agglomeration of multilayered graphene and carbon nanotubes in polymers

• Rasheed Atif and
• Fawad Inam

Beilstein J. Nanotechnol. 2016, 7, 1174–1196, doi:10.3762/bjnano.7.109

• osteoblasts (bone-forming cells) and neurons, and found to be effective nano-carriers for several biomolecules such as proteins, DNA and carbohydrates [4]. Recently, MLG/CNT–polymer nanocomposites have been explored as scaffolds for cell growth and load-bearing implant materials for replacing defective human

Comparison of the interactions of daunorubicin in a free form and attached to single-walled carbon nanotubes with model lipid membranes

• Dorota Matyszewska

Beilstein J. Nanotechnol. 2016, 7, 524–532, doi:10.3762/bjnano.7.46

• to the concentrations used in the in vitro studies. The IC50 value, which is defined as the concentration of a drug that inhibits cell growth by 50%, given in the literature usually varies from 10−6 M to 10−5 M depending on the type of cell lines [32][33][34]. In the next step, voltammograms obtained

Influence of calcium on ceramide-1-phosphate monolayers

• Joana S. L. Oliveira,
• Gerald Brezesinski,
• Alexandra Hill and
• Arne Gericke

Beilstein J. Nanotechnol. 2016, 7, 236–245, doi:10.3762/bjnano.7.22

• kinase [1][2]. Several reviews about the function of C1P describe its role in a number of biological functions as cell growth, survival and mediation of macrophage migration and control of inflammatory responses [3][4][5]. According to the work of Chalfant and co-workers [6][7], C1P mediates inflammatory

The eNanoMapper database for nanomaterial safety information

• Nina Jeliazkova,
• Charalampos Chomenidis,
• Philip Doganis,
• Bengt Fadeel,
• Roland Grafström,
• Barry Hardy,
• Janna Hastings,
• Markus Hegi,
• Vedrin Jeliazkov,
• Nikolay Kochev,
• Pekka Kohonen,
• Cristian R. Munteanu,
• Haralambos Sarimveis,
• Bart Smeets,
• Pantelis Sopasakis,
• Georgia Tsiliki,
• David Vorgrimmler and
• Egon Willighagen

Beilstein J. Nanotechnol. 2015, 6, 1609–1634, doi:10.3762/bjnano.6.165

• average, though this is not always specified), a minimum and maximum value, or a single value and a standard deviation. Biological measurements are linked to assays (such as cytotoxicity, cell growth, cell viability, genotoxicity, and oxidative stress), endpoints measured on that assay (e.g., ROS