Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• -regulated. The role of TFF1 in cancer remains controversial but many reports have demonstrated that TFF1 serves as a tumor suppressor gene that inhibits cancer cell proliferation and migration in epithelial cancers such as gastric, breast and pancreatic cancer [36]. Recent experimental evidence has revealed

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• plates and left to adhere overnight (5% v/v CO2 in air, 37 °C). Cells were then exposed to 0.25 mL of nanoparticle suspensions in full medium (concentration: 0.01–0.5 mg/mL) for 24 h, then determining viability using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS assay). Briefly

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

• Dávid Juriga,
• Evelin Sipos,
• Orsolya Hegedűs,
• Gábor Varga,
• Miklós Zrínyi,
• Krisztina S. Nagy and
• Angéla Jedlovszky-Hajdú

Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249

• established. Using metoprolol as a model drug, cell proliferation and drug release kinetics were studied at different LYS contents and in the presence of thiol groups. The optimal ratio of cross-linkers for the proliferation of periodontal ligament cells was found to be 60−80% LYS and 20−40% CYS. The

• day one, the cell viability index was lower for the 20CYS-LYS gel and the 40CYS-LYS gel than for the 100CYS-LYS gel. Still, for both gels, it had doubled after three days of cell growth suggesting that a lower CYS concentration favors cell proliferation. This result is presumably related to the

• role as a cross-linker. This function of LYS in scaffolds, which is related to cell proliferation, has not been investigated before. Nevertheless, the positive effect of LYS is highly concentration-dependent. According to the literature, a high amount of LYS has a cytotoxic effect, while a lower amount

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• (Figure 1c). In contrast, exposure to cystabn, reduced cell proliferation over a 5 day period (Figure 1d), thus confirming that the addition of an N terminal cysteine did not interfere with GRPR binding. Exposure of NCI-H82 cells to the Tyr4-Bn agonist (Figure 1e) and cystabn antagonist peptide (Figure 1f

• ) caused no change in cell growth. These cell proliferation studies were performed in serum-free conditions in line with previous studies [24][25] for two principle reasons. Firstly, the removal of serum from the culture medium depletes bovine bombesin-like peptides that could otherwise stimulate cell

• GRPR depleted NCI-H82 cells. In contrast to results from NCI-H345 cells, the addition of Tyr4-Bn to NCI-H82 caused no noticeable increase in cell proliferation and cystabn effected no reduction in proliferation. This demonstrated that cystabn functionally targeted GRPR expressing cells in a specific

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• or better antiproliferative effects after a 72 h incubation. The DiP and P2P were made up of drugs and phosphatidylcholine. The antiproliferative activities of DiP and P2P against A549 and PC9 cells originated from the drugs. Therefore, there is no huge difference in cell proliferation inhibition

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

• Xianping Liu,
• Chengjuan Du,
• Haichun Li,
• Ting Jiang,
• Zimiao Luo,
• Zhiqing Pang,
• Daoying Geng and
• Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

• Sigma (USA) and Cy7.5 NHS ester was purchased from Nanocs (USA). Fetal bovine serum (FBS), phosphate buffered saline (PBS), trypsin-EDTA (0.25%), high glucose Dulbecco’s modified Eagle’s medium (DMEM) and penicillin-streptomycin were purchased from Gibco (CA, USA). The MTT cell proliferation and

Nanoarchitectonics meets cell surface engineering: shape recognition of human cells by halloysite-doped silica cell imprints

• Elvira Rozhina,
• Ilnur Ishmukhametov,
• Svetlana Batasheva,
• Farida Akhatova and
• Rawil Fakhrullin

Beilstein J. Nanotechnol. 2019, 10, 1818–1825, doi:10.3762/bjnano.10.176

• occurs. However, flow cytometry-based cell proliferation monitoring performed with cells stained with 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFSE) dye has confirmed that cells coated with pure silica or silica/halloysite display a similar cell proliferation pattern as intact HeLa cells

Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• –brain barrier using rat brain capillary endothelial cells (rBCEC4). All types of nanoparticles were taken up time-dependently by the rBCEC4 cells, albeit to a different extent, causing a time- and concentration-dependent decrease in cell viability. Nanoparticle exposure did not change cell proliferation

• cytotoxicity in HUVECs. Furthermore, Si-NPs were shown to induce oxidative stress and inflammation mediated by mitogen-activated protein kinase (MAPK) and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) [21] pathways that are related to cell proliferation and differentiation but also to

• had a significant effect on the expression levels of Akt or NF-κB or their respective phosphorylated forms indicating that the NPs do not modulate cell proliferation and inflammation. The fact that the MTT assay measures the metabolic activity of a cell might explain the lack of alterations in markers

Biocompatible organic–inorganic hybrid materials based on nucleobases and titanium developed by molecular layer deposition

• Leva Momtazi,
• Henrik H. Sønsteby and
• Ola Nilsen

Beilstein J. Nanotechnol. 2019, 10, 399–411, doi:10.3762/bjnano.10.39

• of the same samples was measured after each period of water treatment. The three hour and four day time periods are the durations in which cells were cultured on these substrates for cell attachment and cell proliferation assays, respectively, in our previous study [23]. Unlike for the Ti-amino acids

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• GmbH, Heidelberg, Germany). The cells were maintained at 37 °C in a humidified 5% CO2 atmosphere and sub-cultivated every 7–14 days, depending on the cell proliferation. Adherent cells were washed with phosphate-buffered saline solution (PBS, GIBCO, Invitrogen GmbH) and detached from the culture flasks

Size-selected Fe₃O₄–Au hybrid nanoparticles for improved magnetism-based theranostics

• Maria V. Efremova,
• Yulia A. Nalench,
• Eirini Myrovali,
• Anastasiia S. Garanina,
• Ivan S. Grebennikov,
• Polina K. Gifer,
• Maxim A. Abakumov,
• Marina Spasova,
• Makis Angelakeris,
• Alexander G. Savchenko,
• Michael Farle,
• Natalia L. Klyachko,
• Alexander G. Majouga and
• Ulf Wiedwald

Beilstein J. Nanotechnol. 2018, 9, 2684–2699, doi:10.3762/bjnano.9.251

• , and 20 μL of MTS reagent (CellTiter 96 aqueous non-radioactive cell proliferation assay, Promega, USA) was added to each well. Following 4 h of incubation at 37 °C in darkness, the wells were placed on a permanent magnet to remove the NPs from solution, and 100 μL of the obtained solution was

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• Zdenek Plichta Yulia Kozak Rostyslav Panchuk Viktoria Sokolova Matthias Epple Lesya Kobylinska Pavla Jendelova Daniel Horak Institute of Macromolecular Chemistry CAS, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• degradation pattern of which is appropriate for this application, contributed positively to the anti-inflammatory action of dexamethasone and not to its immunosuppressive action. Cytocompatibility behavior The MTT assay shows the quantitative analysis of cell proliferation as a function of the time. The MTT

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1

• . Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning. Keywords: cell attachment; cell proliferation; dental implants; gelatin; genipin; nanopatterning; Introduction Topography on the micro- and nanoscale is an important property

• nanogroove, with widths ranging from 333 to 650 nm, increased cell proliferation, as well as the initial cell attachment of smooth muscle cells, better than a planar collagen surface. These findings indicate that cell type, as well as pattern shape and size, can influence the efficiency of cell attachment

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• instance, piperine, a major chemical compound present in black pepper, has shown anti-inflammatory, anti-angiogenic, and anti-arthritic effects [15]. Moreover, it has been reported that black pepper is capable to reduce breast cancer cell proliferation [16][17][18]. Turmeric is an abundant medicinal herb

Humidity-dependent wound sealing in succulent leaves of Delosperma cooperi – An adaptation to seasonal drought stress

• Olga Speck,
• Mark Schlechtendahl,
• Florian Borm,
• Tim Kampowski and
• Thomas Speck

Beilstein J. Nanotechnol. 2018, 9, 175–186, doi:10.3762/bjnano.9.20

• , reversible cross-linking) are involved, a transfer to technology is especially promising. However, if biological mechanisms such as metabolism-driven modifications (e.g., biosynthesis, cell proliferation) are present, the technical application is much more difficult. A clear distinction must also be made

• mechanically driven mechanism. Subsequent to the relatively fast initial self-sealing processes, self-healing including biosynthesis and cell proliferation starts, and hence the formation of a specific wound healing tissue. After a few weeks, self-healing is completed showing pronounced healed tissue in the

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• changes in the cells and tissues. Some of these events include cell proliferation and apoptosis [1], ion transport [2] and other cellular process and diseases such as cancer [3][4][5], Parkinson's, and Alzheimer's disease [6]. Despite the large number of nanotechnology platforms available to date for

• (Figure 8B,C), a progressive accumulation of fluo-CNOs was observed during the cell proliferation. Additionally, the efficient uptake was supported by a three dimensional reconstruction of cells treated with CNOs (Figure 9). Finally, we demonstrated that the on/off fluorescent emission properties of the

• a positive control for cytotoxicity, the cells were incubated with 5% DMSO. Cell viability was determined using the cell proliferation reagent WST-1 (Roche Applied Sciences). After aspiration of the culture medium, a mixture of DMEM and WST1 reagent (1/10 volume) was added to each well. After 2 h of

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• human breast cancer cell line. For this purpose, MCF-7 cells were incubated with different concentrations of PCX in dimethyl sulfoxide (DMSO). Non-treated cells were incubated with DMEM alone and were used as control group. Cell proliferation was determined and the IC50 value of PCX was calculated and

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• Cellular viability was quantified in quintuplicate in 96-well culture plates using the cell proliferation reagent WST-1 (Roche, Mannheim, Germany), for which no interaction between the water-soluble formazan dye and CNTs has been observed [32]. WST-1 reagent was added to the cells 72 h after end of

• treatment according to the instructions of the manufacturer. Absorbance was measured with a spectrophotometer (Anthos labtec, Krefeld, Germany) at 450 nm with 620 nm as reference wavelength and was directly proportional to the viability of the cells. Cell proliferation, clonogenic survival and cell death

• rate For examination of cell proliferation, cell colony formation and apoptosis cells were seeded into 6-well plates and incubated as indicated above. Seventy two hours after the end of treatment cells were harvested by trypsin/EDTA treatment and submitted to the aforementioned assays. Cell

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• observations have been reported in the study of mouse embryonic stem cells, where QD loss was still detected after the inhibition of cell proliferation, suggesting that QDs might be excreted from cells [50]. Indeed, we demonstrated that MSCs could be repetitively labelled by the removal of supernatants from QD

Silicon microgrooves for contact guidance of human aortic endothelial cells

• Sara Fernández-Castillejo,
• Pilar Formentín,
• Úrsula Catalán,
• Josep Pallarès,
• Lluís F. Marsal and
• Rosa Solà

Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72

• not observed and the cell spreading seems lower than after 2 days of incubation (data not shown). Taking all these data together, it seems safe to assure that the presence of microstructure affects cell morphology, irrespective of the patterned employed. Cell proliferation The effect of surface

• alignment in the direction of the groove pattern, accordingly to the concept of contact guidance, and circularity and filopodia were reduced on patterned substrates when compared to flat substrates. Lastly, cell proliferation was found to be lower on patterned substrates, surely because of the

• presence of filopodia. Circularity was calculated as the ratio between the minimum and maximum diameters. Values range from 0 to 1, where 0 represents an elongated cell and 1 a perfect circular shape. Alignment and presence of filopodia was estimated by visual assessment. Cell proliferation was calculated

Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring

• Niina Halonen,
• Joni Kilpijärvi,
• Maciej Sobocinski,
• Timir Datta-Chaudhuri,
• Antti Hassinen,
• Someshekar B. Prakash,
• Peter Möller,
• Pamela Abshire,
• Sakari Kellokumpu and
• Anita Lloyd Spetz

Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179

• cell proliferation thus seemed to be normal, the use of the LTCC package for the sensor chip was regarded as promising [23]. Here we have tested version 2 of the LTCC package made from DupontTM 951 LTCC tape instead of the Heraeus HeraLock® Tape HL2000 (no longer produced) used in our earlier version

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• and their biological responses to micro- and nanostructured surfaces are reviewed. Emphasis is given to studies of cell morphology and motility, cell proliferation, the cytoskeleton and cell-matrix adhesions, and signal transduction pathways of vascular cells. We finalize with a short outlook on

• proliferation rate of ECs on colloidal silica-coated surfaces with nanoroughness was decreased compared to ECs on flat surfaces [43]. For both types of vascular cells it is not clear through which mechanism the cell proliferation is influenced by the surface topography. Therefore, more research has still to be

• performed in these cell types to determine how substrate shape and feature dimensions correlate with cell proliferation. 2.2 Structured surfaces influence the morphology, adhesion, and motility of vascular cells Cells cultured on micro- and nanostructured substrates tend to change their morphology and their

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• biocompatibility was satisfactory. No detrimental effect on viability or proliferation of NSCs was observed, as compared with unlabeled control cells. Contrarily to that, the efficient concentration of nanomag®-D-spio (4 mg/mL) needed to achieve cell labeling, scored low (<80%) in a cell proliferation rate as