Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

Ultrathin water layers on mannosylated gold nanoparticles

• Maiara A. Iriarte Alonso,
• Jorge H. Melillo,
• Silvina Cerveny,
• Yujin Tong and
• Alexander M. Bittner

Beilstein J. Nanotechnol. 2025, 16, 2183–2198, doi:10.3762/bjnano.16.151

• light scattering, and infrared spectroscopy. We probed particles adsorbed on hydrophilic and hydrophobic surfaces with atomic force microscopy (AFM) and vibrational sum frequency generation (VSFG) spectroscopy, both operated under variable air humidity. For AFM, we additionally tested hydrophilic and

• short oligo(ethylene glycol) chains. The particles were first characterized by dynamic light scattering (DLS) and zeta potential (ZP) measurements in solution, and by scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM) in vacuum. Samples were adsorbed on flat

• PEG thiol with carboxylic acid termination was used. To obtain the dimanno-AuNPs, 50 mol % of the same carboxylic acid ligand was mixed with 50 mol % of a dimannoside (Manα1-2Man) thiourea PEG thiol. After purification and lyophilization, the nanoparticles were obtained as brown powders. Dynamic light

Quality by design optimization of microemulsions for topical delivery of Passiflora setacea seed oil

• Daniel T. Pereira,
• Douglas Dourado,
• Danielle T. Freire,
• Dayanne L. Porto,
• Cícero F. S. Aragão,
• Myla L. de Souza,
• Guilherme R. S. de Araujo,
• Ana Maria Costa,
• Wógenes N. Oliveira,
• Anne Sapin-Minet,
• Éverton N. Alencar and
• Eryvaldo Sócrates T. Egito

Beilstein J. Nanotechnol. 2025, 16, 2116–2131, doi:10.3762/bjnano.16.146

• desirable nanostructural features over time [39]. It is important to note, however, that NaCMC forms polymeric networks when dispersed in aqueous media, which scatter light and may artificially increase the measured hydrodynamic diameter [40]. Consequently, dynamic light scattering (DLS) data may not

• microemulsions were determined by dynamic light scattering using a Zetasizer® Nano ZS (Malvern Panalytical Ltd. – Malvern, United Kingdom) at 25 °C with a fixed backscattering angle of 173°. Prior to analysis, samples were diluted in purified water (1:40 v/v) [13]. All measurements were performed in triplicate

Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation

• Michał Bartkowski,
• Francesco Calzaferri and
• Silvia Giordani

Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144

• instance, small carbon nanoparticles (CNPs) are readily cleared through renal pathways, whereas larger or irregularly shaped CNPs tend to accumulate in organs such as the liver and spleen. Characterisation of these properties is typically performed using dynamic light scattering, electron microscopy, or

Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• using dynamic light scattering (DLS) with a Zetasizer Nano ZS (Malvern Instruments, UK) at 25 °C. The measurements were based on a refractive index of 1.34 for the material. Prior to analysis, the samples were diluted at a ratio of 1:10 (βCp/distilled water). The reported parameters represent the

Phytol-loaded soybean oil nanoemulsion as a promising alternative against Leishmania amazonensis

• Victória Louise Pinto Freire,
• Mariana Farias Alves-Silva,
• Johny W. de Freitas Oliveira,
• Matheus de Freitas Fernandes-Pedrosa,
• Alianda Maira Cornélio,
• Marcelo de Souza-Silva,
• Thayse Silva Medeiros and
• Arnóbio Antônio da Silva Junior

Beilstein J. Nanotechnol. 2025, 16, 1826–1836, doi:10.3762/bjnano.16.126

• performed. The results revealed nanodroplets with spherical and oval shapes and a homogeneous size distribution, with diameters up to 250 nm, consistent with the dynamic light scattering (DLS) data (Figure 1c,d). Colloidal and physicochemical stability The colloidal and physicochemical stability of the NEs

• analysis. All samples were prepared in triplicate and data were expressed as mean ± standard deviation. The NEs composition was described in Table 3. Physicochemical characterization Droplet size, polydispersity index and zeta potential evaluation The droplet size was determined by dynamic light scattering

Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• mL·min−1, using sealed aluminum pans (T181206 and T181128). Droplet size, polydispersity index, zeta potential, and conductivity Droplet size and polydispersity index (PdI) of the formulations were measured 24 h after preparation using dynamic light scattering with a Zetasizer Nano-ZS ZEN 3600 (Malvern

• . This difference can be attributed to two main factors: (1) the significant reduction in droplet size and (2) the improved uniformity of the nanodroplets. These factors minimize light scattering, resulting in a more transparent nanoemulsion. The macroscopic difference between the ophthalmic

Prospects of nanotechnology and natural products for cancer and immunotherapy

• Jan Filipe Andrade Santos,
• Marcela Bernardes Brasileiro,
• Pamela Danielle Cavalcante Barreto,
• Ligiane Aranha Rocha and
• José Adão Carvalho Nascimento Júnior

Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116

• the development of reactive oxygen species (ROS) [118]. Hyaluronic acid can inhibit local recurrence and distant tumor growth when used in immunotherapy scenarios in conjuction with CAR-T cells and anti-PDL1-conjugated platelets [119]. Transmission electron microscopy (TEM) and dynamic light

• scattering (DLS), and UV–visible (UV–vis) spectroscopy were used for characterization. The nanoparticles showed increased cellular uptake compared to free PD-L1, suppression of the NF-κB pathway as indicated by reduced PHO-P65 protein expression, and enhanced tumor inhibition due to immune activation and

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• polyethylenimine for loading peptides and proteins of T. serrulatus venom, and their use as a potential immunoadjuvant was evaluated. The protein loading efficiency of about 100% and the polyacrylamide gel electrophoresis assay confirmed the success of venom loading. Dynamic light scattering and zeta potential

• shape, uniform aspects, and sizes compatible with the results obtained by dynamic light scattering. These morphological features are known to influence the biological activity of nanoparticles loaded with bioactive molecules, directly affecting their release. The release profile of a substance depends

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• by dynamic light scattering. The antibacterial activity of these nanoparticles was evaluated against Gram-positive and Gram-negative bacterial strains (S. aureus, S. epidermidis, E. coli, and P. aeruginosa) and compared to the free complex 18. While neither free drug 18 nor the encapsulated

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• light scattering (DLS) is commonly used to determine liposome size and size distribution. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) can be used to image liposome morphology and determine lamellarity. Zeta potential measurements assess the surface charge of liposomes, which

• liposomes is preserved [110]. The surface charge of liposomes, often expressed as zeta potential, is an important parameter that influences their stability and interaction with biological systems. Electrophoretic light scattering is often used to measure the zeta potential of liposomes [110][125]. Cryo-TEM

Enhancing the photoelectrochemical performance of BiOI-derived BiVO₄ films by controlled-intensity current electrodeposition

• Huu Phuc Dang,
• Khanh Quang Nguyen,
• Nguyen Thi Mai Tho and
• Tran Le

Beilstein J. Nanotechnol. 2025, 16, 1289–1301, doi:10.3762/bjnano.16.94

• spectrum, (b) bandgap energies of BiVO4(146), BiVO4(224), BiVO4(226), BiVO4(324), and BiVO4(326) photoanodes. Minor fluctuations above 500 nm are due to light scattering from porous films. Raman spectrum of BiVO4(146), BiVO4(224), BiVO4(226), BiVO4(324), and BiVO4(326) photoanodes. (a) LSV curves, (b) ABPE

Acrocomia aculeata oil-loaded nanoemulsion: development, anti-inflammatory properties, and cytotoxicity evaluation

• Verónica Bautista-Robles,
• Hady Keita,
• Edgar Julián Paredes Gamero,
• Layna Tayná Brito Leite,
• Jessica de Araújo Isaías Muller,
• Mônica Cristina Toffoli Kadri,
• Ariadna Lafourcade Prada and
• Jesús Rafael Rodríguez Amado

Beilstein J. Nanotechnol. 2025, 16, 1277–1288, doi:10.3762/bjnano.16.93

• , maintaining consistent zeta potential and droplet size parameters over a 180-day storage period at 25 ± 2 °C. Dynamic light scattering analysis revealed a mean nanodroplet size (by intensity) of 173.6 ± 0.70 nm (Figure 2A). The nanoemulsion, composed of 0.28 parts of Span 80® and 0.72 parts of Tween 80

• the smallest droplet size was selected as the (HLBr) to emulsify bocaiúva oil [63]. Droplet size and zeta potential Droplet size and polydispersity index (PDI) were measured by dynamic light scattering (DLS) with a Zetasizer Nano-ZS instrument (Malvern, UK). Zeta potential was determined by

• electrophoretic light scattering with a Zetasizer Nano-ZS instrument (Malvern, UK). AANE was diluted with Milli-Q water (1:25, v/v). All measurements were performed in triplicate, and results were presented as the mean ± standard deviation [42]. Shelf stability The selected AANE was transferred to an amber vial

Investigation of the solubility of protoporphyrin IX in aqueous and hydroalcoholic solvent systems

• Michelly de Sá Matsuoka,
• Giovanna Carla Cadini Ruiz,
• Marcos Luciano Bruschi and
• Jéssica Bassi da Silva

Beilstein J. Nanotechnol. 2025, 16, 1209–1215, doi:10.3762/bjnano.16.89

• , complementary methods may be performed together, such as dynamic light scattering [26] and turbidimetry [27]. Furthermore, TEM analysis can provide a more accurate understanding of the incorporation of PpIX into the micellar systems, overcoming the limitations associated with spectrophotometric estimations

• . These limitations include potential overestimation of solubility due to light scattering by aggregates, turbidity-related interference, and the inability to distinguish between molecularly dissolved and aggregated species [28]. Therefore, TEM micrographs of PpIX samples in different media containing

Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti

• Maria A. A. Bertonceli,
• Vitor D. C. Cristo,
• Ivo J. Vieira,
• Francisco J. A. Lemos,
• Arnoldo R. Façanha,
• Raimundo Braz-Filho,
• Gustavo V. T. Batista,
• Luis G. M. Basso,
• Sérgio H. Seabra,
• Thalya S. R. Nogueira,
• Felipe F. Moreira,
• Arícia L. E. M. Assis,
• Antônia E. A. Oliveira and
• Kátia V. S. Fernandes

Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88

• , controls), while the other corresponded to nanocomposites containing rotenoids (CS/TPP rot and CS/TPP–β-CD rot). Size, polydispersity, and zeta potential The average size (in nm) and PDI of the nanoparticles were measured using dynamic light scattering (DLS) on the Litesizer DLS 500 instrument (Anton Paar

Mechanical stability of individual bacterial cells under different osmotic pressure conditions: a nanoindentation study of Pseudomonas aeruginosa

• Lizeth García-Torres,
• Idania De Alba Montero,
• Eleazar Samuel Kolosovas-Machuca,
• Facundo Ruiz,
• Sumati Bhatia,
• Jose Luis Cuellar Camacho and
• Jaime Ruiz-García

Beilstein J. Nanotechnol. 2025, 16, 1171–1183, doi:10.3762/bjnano.16.86

• . Using forward light scattering experiments, Çetiner et al. reported that the time required for PA to reach an equilibrium state after osmolyte release when exposed to drastic osmotic downshocks was about 150 ms [14]. In our experiments, PA was tested in different conditions after the solution was

Towards a quantitative theory for transmission X-ray microscopy

• James G. McNally,
• Christoph Pratsch,
• Stephan Werner,
• Stefan Rehbein,
• Andrew Gibbs,
• Jihao Wang,
• Thomas Lunkenbein,
• Peter Guttmann and
• Gerd Schneider

Beilstein J. Nanotechnol. 2025, 16, 1113–1128, doi:10.3762/bjnano.16.82

• anomalous diffraction solution of the Mie theory is equivalent to a simplified form of the parabolic wave equation known as the eikonal approximation in light scattering [28]: The eikonal approximation SEA(ψ) for the parabolic wave equation holds under the same two conditions required for the Mie theory to

Influence of ion beam current on the structural, optical, and mechanical properties of TiO₂ coatings: ion beam-assisted vs conventional electron beam evaporation

• Agata Obstarczyk and
• Urszula Wawrzaszek

Beilstein J. Nanotechnol. 2025, 16, 1097–1112, doi:10.3762/bjnano.16.81

• high refractive index and thickness up to 400 nm are required. Although the density and the refractive index of crystalline TiO2 are higher than those of the amorphous phase, it is often preferred in optical devices due to its lower surface roughness and light scattering [65]. Nanoindentation

Fabrication of metal complex phthalocyanine and porphyrin nanoparticle aqueous colloids by pulsed laser fragmentation in liquid and their potential application to a photosensitizer for photodynamic therapy

• Taisei Himeda,
• Risako Kunitomi,
• Ryosuke Nabeya,
• Tamotsu Zako and
• Tsuyoshi Asahi

Beilstein J. Nanotechnol. 2025, 16, 1088–1096, doi:10.3762/bjnano.16.80

• within the nanoparticles. The hydrodynamic diameter of the colloidal dispersion was examined by dynamic light scattering (DLS) measurements, and the Z-average value was 96 nm (see Figure S2, Supporting Information File 1). The fabricated nanoparticles remained stably dispersed over a period of one month

• microcrystalline powder into small nanoparticles with high efficiency. The particle size was determined by dynamic light scattering measurements (Zetasizer nanoS, Malvern Instruments). In this analysis, the prepared colloids were diluted more than tenfold with ion-exchanged water. Dispersion stability Dispersion

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• -Vis-NIR) measured optical properties across the 200–800 nm range, while dynamic light scattering (DLS, Malvern Panalytical) provided data on hydrodynamic size and zeta potential after dispersing 1 mg of material in 1 mL of deionized water and sonicating for 30 min. Finally, piezoresponse force

Soft materials nanoarchitectonics: liquid crystals, polymers, gels, biomaterials, and others

• Katsuhiko Ariga

Beilstein J. Nanotechnol. 2025, 16, 1025–1067, doi:10.3762/bjnano.16.77

A calix[4]arene-based supramolecular nanoassembly targeting cancer cells and triggering the release of nitric oxide with green light

• Cristina Parisi,
• Loredana Ferreri,
• Tassia J. Martins,
• Francesca Laneri,
• Samantha Sollima,
• Antonina Azzolina,
• Antonella Cusimano,
• Nicola D’Antona,
• Grazia M. L. Consoli and
• Salvatore Sortino

Beilstein J. Nanotechnol. 2025, 16, 1003–1013, doi:10.3762/bjnano.16.75

• typical features of the ABF chromophore [55][56][57][58][59][62][63][64] with a maximum at ca. 480 nm (Figure 1A). However, dynamic light scattering (DLS) measurements evidenced that, in line with its amphiphilic character, compound 1 is not present in the monomeric form but as nanoaggregates ca. 180 nm

• equation: Dynamic light scattering measurements were performed on a ZetaSizer NanoZS90 (Malvern Instrument, UK), equipped with a 633 nm laser, at a scattering angle of 90° and at 25 °C. In a manner analogous to [70], photolysis experiments were performed by irradiating the samples in solution in a

Time-resolved probing of laser-induced nanostructuring processes in liquids

• Maximilian Spellauge,
• David Redka,
• Mianzhen Mo,
• Changyong Song,
• Heinz Paul Huber and
• Anton Plech

Beilstein J. Nanotechnol. 2025, 16, 968–1002, doi:10.3762/bjnano.16.74

• ]. Light scattering becomes relevant if the structures exceed a size of a fraction of the wavelength [126][127][128]. Figure 8A shows several schemes of exciting NPs by focused laser beams for excitation and probing. Both coaxially and side illumination allow to image the scattering emitted from induced

Morphology and properties of pyrite nanoparticles obtained by pulsed laser ablation in liquid and thin films for photodetection

• Akshana Parameswaran Sreekala,
• Bindu Krishnan,
• Rene Fabian Cienfuegos Pelaes,
• David Avellaneda Avellaneda,
• Josué Amílcar Aguilar-Martínez and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 785–805, doi:10.3762/bjnano.16.60

• a very reflective surface, the percentage reflectance was measured and is given in Supporting Information File 1, Figure S2c. Estimating the bandgap values of the films was challenging due to light scattering [8]. For homogenous and smooth deposition by EPD, it is crucial that the particles in the

Serum heat inactivation diminishes ApoE-mediated uptake of D-Lin-MC3-DMA lipid nanoparticles

• Demian van Straten,
• Luuk van de Schepop,
• Rowan Frunt,
• Pieter Vader and
• Raymond M. Schiffelers

Beilstein J. Nanotechnol. 2025, 16, 740–748, doi:10.3762/bjnano.16.57

• Slide-A-Lyzer, MWCO 20,000, 0.5–3 mL, dark, 4 °C). LNP stocks were stored at 4 °C in the dark. LNP characterization The hydrodynamic diameter of LNPs was determined via dynamic light scattering (DLS). LNP stocks were diluted 1:20 in PBS and analyzed at 25 °C using a ZetaSizer Nano ZS 90 (Malvern