Synthesis, docking study and biological evaluation of ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones as potential inhibitors of the mycobacterial galactan synthesis targeting the galactofuranosyltransferase GlfT2

  1. Marek Baráth1ORCID Logo,
  2. Jana Jakubčinová1,
  3. Zuzana Konyariková2,
  4. Stanislav Kozmon1ORCID Logo,
  5. Katarína Mikušová2ORCID Logo and
  6. Maroš Bella1ORCID Logo

1Institute of Chemistry Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38 Bratislava, Slovakia
2Department of Biochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovičova 6, SK-842 15 Bratislava, Slovakia

  1. Corresponding author email

The article is dedicated to Prof. Ladislav Petruš on the occasion of his 70th birthday.

Associate Editor: J. S. Dickschat
Beilstein J. Org. Chem. 2020, 16, 1853–1862. doi:10.3762/bjoc.16.152
Received 30 Apr 2020, Accepted 09 Jul 2020, Published 27 Jul 2020

Abstract

A series of ten novel ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones bearing a 1-O-phosphono moiety and three different substituents at C-2 has been prepared. Due to the structural similarities of these scaffolds to the native substrate of mycobacterial galactofuranosyltransferase GlfT2 in the transition state, we evaluated these compounds by computational methods, as well as in an enzyme assay for the possible inhibition of the mycobacterial galactan biosynthesis. Our data show that despite favorable docking scores to the active site of GlfT2, none of these compounds serve as efficient inhibitors of the enzymes involved in the mycobacterial galactan biosynthesis.

Keywords: GlfT2; molecular modeling; mycobacterium tuberculosis; synthesis; transition state inhibitors

Introduction

Tuberculosis (TB) is one of the most prevalent infectious diseases, with estimated 10 million new cases each year. It causes about 1.5 million deaths annually and is amongst the top 10 causes of death worldwide. The emergence of multidrug-resistant and extensively drug-resistant TB has become a major public health concern as the success rate of the treatment in these cases is only 56% and 39%, respectively [1]. Clearly, the need for new and more efficient TB drugs is pressing. Among the novel therapeutic agents developed for TB, there are several compounds that target the cell wall of Mycobacterium tuberculosis, the causative agent of the disease [2]. The mycobacterial cell wall core is formed by the mycolyl–arabinogalactan–peptidoglycan (mAGP) complex, which provides a highly hydrophobic and resistant barrier surrounding the bacterium [3]. The galactan component of the cell wall consists of polysaccharide chains composed of approximately 22 galactofuranose (Galf) residues linked by alternating β-(1→5)- and β-(1→6)-glycosidic bonds [4]. The Galf monomer is restricted to some bacteria, fungi and a few protozoan species, and it seems to be absent in humans [5]. The enzymes participating in the galactan build-up could thus be considered as potential targets for new antitubercular drug developments [6]. Mycobacterial galactan is synthesized by two bifunctional galactofuranosyltransferases, GlfT1 and GlfT2 (Supporting Information File 1, Figure S1). The former one initiates the galactan biosynthesis by addition of the first two Galf residues to decaprenyl-P-P-GlcNAc-Rha, (glycolipid 2, GL2), which serves as a lipid carrier for arabinogalactan polymerization [7,8]. The latter enzyme, GlfT2, extends the product of GlfT1-catalyzed reaction, decaprenyl-P-P-GlcNAc-Rha-Galf2 (glycolipid 4, GL4), producing the lipid-linked galactan polymer [8]. Both enzymes require an activated sugar donor uridine diphosphate (UDP)-Galf, which is synthesized from UDP-galactopyranose (UDP-Galp) by the enzyme UDP-Galp mutase [9] (Supporting Information File 1, Figure S1).

The recently published GlfT2 X-ray structure with a UDP donor part [10] was used in the reaction mechanism studies using computational chemistry methods. The probable reaction mechanisms were studied by hybrid DFT QM/MM molecular dynamics simulations [11] where the possible transition state (TS) structures were localized. The observation of the possible TS structure opens the opportunities for the in silico based design of possible GlfT2 inhibitors that mimic the TS structure.

The galactofuranosyltransferase GlfT2 is a bisubstrate enzyme with a single catalytic domain and its catalytic reaction transition state shares some structural similarities with the previously modeled N-acetylglucosaminyltransferase I (GnT-I) reaction transition state [11]. Previously, we have designed 2-thiohexofuranoside skeletons bearing a phosphate group in position 1 by molecular modeling as potential transition state inhibitors of human glycosyltransferase I (GnT-I) [12], the bisubstrate enzyme requiring a metal co-factor for the proper synthesis of N-glycans [13]. Subsequently, pioneer structures based on ᴅ-fructofuranose [14], ᴅ-tagatofuranose or ᴅ-psicofuranose [15,16] have been prepared, but their inhibitory activity could not have been tested due to their low stability under standard conditions [16]. Further investigations on their more stable analogs resulted in the synthesis of stable 1-O-phoshono-β-ᴅ-psicofuranosyl sulfone, which was prepared by a simultaneous phosphorylation and oxidation of ethyl 2-thio-β-ᴅ-psicofuranoside with dibenzyl N,N-dimethylphosphoramidite catalyzed by 1H-tetrazole and followed by treatment with an excess of 3-chloroperbenzoic acid [16]. Fortunately, the molecular modeling studies did not bring any significant contention about the negative influence of the SO2 group in the target molecule on the standard molecular docking parameters [16]. Based on this fact, a new generation of stable possible inhibitors of GnT-I, comprising structures derived from the ᴅ-fructofuranose skeleton bearing an SO2 group and sulfated at the position C-1 were prepared [17]. However, these compounds did not show any significant inhibitory activity [17]. Therefore, we decided to prepare structures originally suggested by molecular modeling having the 1-O-phosphate group stabilized by anomeric sulfones on the ᴅ-fructofuranose and ᴅ-tagatofuranose skeleton (13, Figure 1). Similarities in the TS structures of GnT-I and GlfT2 prompted us to examine these molecules against GlfT2, a possible target for the development of the drugs against tuberculosis. Despite that the studied structures do not contain the galacto configuration on the furanose ring, the studied compounds mimic the TS structure of the GlfT2 catalytic reaction. The TS structure mimetics are stable compounds designed to mimic the unstable TS complex structure and also mimic its partial charges and charge distribution. Herein, the molecular docking, synthesis and inhibitory activity of ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones 13 against GlfT2 are discussed. Moreover, we extended the scope of the simultaneous phosphorylation and oxidation to 2-thio-ᴅ-fructo- and 2-thio-ᴅ-tagatofuranosides as the key step in the synthesis of target structures.

[1860-5397-16-152-1]

Figure 1: Target ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones 13.

Results and Discussion

Docking study

In the presented study, the compounds based on the fructofuranose and tagatofuranose derivatives (Figure 1) were docked into the GlfT2 structure. The proposed structures nicely follow the probable TS structure of the GlfT2 catalytic reaction as is shown in Supporting Information File 1, Figure S2. The available GlfT2 X-ray structure contains only the UDP part of the native UDP-Galf donor substrate (PDB ID: 4FIY). The structure of the UDP-Galf was docked into the GlfT2 structure to obtain the docking score of the native substrate. The observed UDP-Galf docking score serves as a reference for the studied compounds. The obtained UDP-Galf docking pose showed almost the same binding mode of the UDP moiety as UDP present in the X-ray structure (Supporting Information File 1, Figure S3). A small movement was observed only for the diphosphate part due to the proper accommodation of the Galf moiety within the GlfT2 binding pocket. This suggested that the UDP-Galf docked binding pose was reliable and could be used as a reference for the docking of the studied structures. All fructofuranose and tagatofuranose derivatives were docked into the GlfT2 structure obtained by QM/MM molecular dynamics simulations, which representes the structure close to the transition state structure of the GlfT2 catalytic reaction. The best ten docking poses of each docked molecule were saved for further analyses.

The docking predicted one or two main binding modes for each docked molecule. The most populated binding mode also contained the binding pose with the lowest docking score (for which the highest affinity was predicted). Only the binding modes with the lowest docking score will be discussed below. The observed binding modes of the docked molecules revealed some common interaction patterns. Interactions of the PO4 group with a metal ion and arginine R171 were observed in almost all docking poses and in some cases the C2 hydroxy group also interacted with a metal ion. The C5 hydroxy group very often interacts with the catalytic base aspartate D372 side chain. Moreover, in the poses where this interaction has been found, the C5 hydroxy group creates a hydrogen bond with the ammonium group of the lysine K369 residue. In many docking poses the interaction of the C3 hydroxy group with the aspartate D256 has also been seen. The SO2 unit attached to alkyl/aryl substituents have been found in two main binding modes. In one binding mode the alkyl/aryl part is bound towards the uridine binding pocket of the UDP-Galf. The second binding mode was found in the place where the acyclic tail of the native Galf is bound. The binding poses with the highest docking score of the four compounds with the highest predicted affinity are shown in Figure 2. The highest binding affinity was predicted for compounds 1bα, 2c, 3c and 1cβ. It can be seen that the compounds 2c, 3c and 1cβ share the common binding interactions described above. However, the 1bα binding mode slightly differed from the modes of the other compounds. In this case the PO4 and hydroxy group on C5 interacted with a metal ion and the hydroxy groups on C2 and C3 created the hydrogen bond with the tyrosine Y236 hydroxy group and the phenyl ring created a cation–π stacking interaction with the arginine R171 side chain (Figure 2).

[1860-5397-16-152-2]

Figure 2: Molecular representation of the best binding poses of the four compounds with the predicted highest affinity. (A) 1bα, (B) 2c, (C) 3c and (D) 1cβ. The molecule–GlfT2 interactions are depicted as dashed lines (yellow – hydrogen bonds, magenta – salt bridge, green – cation/π interaction). The Mg2+ ion is shown as a purple sphere.

The observed theoretical binding energy in kcal/mol represented by the docking scores and the predicted binding affinities for the UDP-Galf, fructofuranose, and tagatofuranose compounds are listed in Table 1.

Table 1: Observed docking score and predicted Ki values of the docked compounds 13.

Compound Docking score [kcal/mol] Predicted Ki [µM]
UDP-Galf −9.192 0.333a
1aα −7.505 5.148
1bα −8.678 0.767
1cα −7.952 2.492
1cβ −8.648 0.805
2a −7.701 3.745
2b −8.388 1.228
2c −8.389 1.226
3a −6.382 31.839
3b −6.900 13.739
3c −8.549 0.946

aExperiment: 250 or 380 µM at 37 °C [10,18,19].

Firstly, the UDP-Galf native donor substrate was docked into the GlfT2 crystal structure to observe the reference docking score and to predict the Ki value for the native donor substrate. The predicted Ki value of the UDP-Galf is 0.333 µM, however, the experimentally observed Km values of the UDP-Galf binding were 250 or 380 µM at 37 °C [10,18,19]. All the predicted values were calculated for this temperature. This observation suggested that the calculated docking score overestimated the predicted binding affinities by three orders of magnitude. Unfortunately, the experimental Km or Ki values of the structures similar to the studied compounds were not available and therefore we were not able to create a more precise linear regression model for the binding affinity prediction. For this reason, we could use only a coarse correction for the calculated Ki values and the experimental Ki values can be expected in the mM and sub-mM range rather than in the µM and sub-µM range. However, the obtained predicted Ki values were reliable for the comparison of the fructofuranose and tagatofuranose compounds binding affinities with the UDP-Galf binding affinity. Moreover, the Ki values gave a more realistic image about the affinity differences between the compounds than only the predicted binding energies. The observed docking score of the studied compounds ranged from −6.4 to −8.7 kcal/mol. All these values were higher than the observed docking score for the UDP-Galf. This means that the docking predicted a lower affinity for the studied compound than for the native substrate. Recalculation of the observed docking scores to the Ki values showed that the predicted affinity of the studied compounds ranged from 0.7 µM to 32 µM. In case of the best four compounds with the highest affinity (1bα, 2c, 3c, 1cβ) the Ki values ranged up to 1.2 µM. The predicted affinity of these compounds was just three to four times lower than the predicted affinity for the UDP-Galf. Such observation is very promising, due to the fact that the studied molecules were smaller, they did not show as many interactions as the native donor substrate and created only one strong interaction with the metal ion cofactor. So further modification of these four molecules can lead to the compounds with similar or higher affinities to the GlfT2 than UDP-Galf.

Chemistry

Within the structure of target compounds 1–3, the carbohydrate skeletons resembled the donor substrate: various RSO2 aglycons mimic an approaching acceptor substrate while the 1-O-phosphono moiety represents the leaving UDP [20].

The synthesis of target compounds 13 consisted of two different strategies. The first one was applied to a series of ᴅ-fructofuranose derivatives 1 and started from the four known alcohols , , and [17]. Initial treatment of these alcohols with commercially available dibenzyl N,N-dimethylphosphoramidite as phosphorylating agent catalyzed by 1H-tetrazole and followed by direct oxidation with an excess of 3-chloroperbenzoic acid (m-CPBA, 12 equiv) led to the simultaneous phosphorylation of the hydroxy group and to the oxidation of the sulfide to give 1-O-dibenzyloxyphosphoryl-ᴅ-fructofuranosyl sulfones , , and in excellent yields (Scheme 1). Final removal of benzyl protecting groups by catalytic hydrogenation provided target 1-O-phosphono-ᴅ-fructofuranosyl sulfones 1aα, 1bα, 1bβ and 1cα as white powders after freeze drying.

[1860-5397-16-152-i1]

Scheme 1: Reagents and conditions: a) 1. (BnO)2P-NMe2, 1H-tetrazole, 0 °C→rt, 1 h, 2. m-CPBA, 0 °C→rt, 1.5‒2 h; b) H2, 10% Pd/C, MeOH, rt, 3‒4 h.

Based on the molecular docking results, the series of ᴅ-tagatofuranose compounds can be divided into two groups. The first group represented the more flexible structures 2 with free hydroxy groups in positions C3 and C4, while the second group of target compounds 3 has hydroxy groups on C3 and C4 protected as acetonides which made their structure more rigid. The synthesis started from the well-known diacetonide 10 [17]. Pivaloylation of the free hydroxy group at C-6 of 10 smoothly gave 11 in good yield. Likewise, 6-O-benzyl derivative 12 was prepared to reduce reaction steps and to ensure a clean progress of the benzyl protecting group removal by catalytic hydrogenation (Scheme 2).

[1860-5397-16-152-i2]

Scheme 2: Reagents and conditions: a) PivCl, pyridine, CH2Cl2, rt, overnight; b) BnBr, NaOH, TBAB, THF, reflux, 3 h.

The direct thioglycosylation of 11 with ethanethiol in the presence of BF3∙OEt2 afforded 2-thio-ᴅ-tagatofuranoside 13 in satisfactory yield (Scheme 3). In the course of the thioglycosylation, only the α-anomer of 13 was detected and isolated as the product. In general, the formation of α-anomers during the thioglycosylation of di-O-isopropylidene-ᴅ-tagatofuranoses 11 and 12 was controlled by the approach of a thiol from exo-face of the bicyclic skeleton. Subsequent simultaneous phosphorylation and oxidation of ethyl 2-thio-ᴅ-tagatofuranoside 13 under identical conditions as used for the fructofuranose pathway provided 1-O-dibenzyloxyphosphoryl-ᴅ-tagatofuranosyl sulfone 14 (Scheme 3). Removal of the pivaloyl protecting group in derivative 14 led to alcohol 15 as the common intermediate for the synthesis of target structures 2a and 3a. The fully deprotected target compound 2a was obtained in good yield by acidic hydrolysis of the acetonide protecting group with 3 M HCl in THF followed by catalytic hydrogenation of 16. On the other hand, direct catalytic hydrogenation of 15 furnished target structure 3a with the hydroxy groups protected on C3 and C4 as an acetonide.

[1860-5397-16-152-i3]

Scheme 3: Reagents and conditions: a) EtSH, BF3∙OEt2, CH2Cl2, 0 °C, 2 h; b) 1. (BnO)2P-NMe2, 1H-tetrazole, 0 °C→rt, 1 h, 2. m-CPBA, 0 °C→rt, 1.5‒2 h; c) MeONa, MeOH, rt, 1.5 h; d) H2, 10% Pd/C, MeOH, rt, 3‒4 h; e) 3 M HCl, THF, 40 °C, 8 h.

Direct thioglycosylation of diacetonide 12 with corresponding thiols (PhSH, iPrSH) led to expected 2-thio-ᴅ-tagatofuranosides 17a and 17b in 44% and 54% yield, respectively. Moderate yields of 2-thio-ᴅ-tagatofuranosides 17 can be explained by cleavage of the benzyl protecting group [21] as well as further side reactions (Scheme 4).

[1860-5397-16-152-i4]

Scheme 4: Reagents and conditions: a) PhSH, or iPrSH, BF3·OEt2, CH2Cl2, −5 °C, 1 h; b) BF3·OEt2, Ac2O, 0 °C, 1.5 h; c) PhSH, BF3·OEt2, CHCl3, −20 °C, 1.5 h.

In order to increase the yield of 2-thio-ᴅ-tagatofuranosides 17, compound 12 was converted to the more reactive diacetate 18 under acetolysis conditions (Ac2O/BF3∙OEt2) [22]. However, besides the expected diacetate 18, 1,2,6-tri-O-acetate 19 was observed and isolated as a byproduct (Scheme 4). The cleavage of the O-benzyl ethers followed by acetylation of the liberated hydroxy groups under acidic conditions was described previously, however, concentrated H2SO4 is usually employed as a promotor in acetic anhydride [23]. Although thioglycosylation of diacetate 18 with PhSH provided 2-thio-ᴅ-tagatofuranoside 20 in 72% yield, the overall synthesis of 17a would contain two more reaction steps and, thus, be less effective. As the yields of 2-thio-ᴅ-tagatofuranosides 17 were comparable to the yield of 2-thio-ᴅ-tagatofuranoside 13 obtained by thioglycosylation of 11, derivative 12 proved to be a more suitable substrate for the synthesis of target compounds 2 and 3 due to the possibility of simultaneous deprotection of the benzyl protecting groups in later stages of the synthesis. This advantage makes the overall synthesis of the target molecules one reaction step shorter in comparison with the synthesis starting from pivalate 11 (Scheme 3). With alcohols 17 in hand, the synthesis continued with their simultaneous phosphorylation and oxidation under the above-mentioned conditions to give 1-O-dibenzyloxyphosphoryl-ᴅ-tagatofuranosyl sulfones 21 (Scheme 5). Next, catalytic hydrogenation of 21 afforded the final acetonides 3b and 3c in excellent yields. The fully deprotected target structures 2b and 2c were obtained by acetonide hydrolysis under acidic conditions followed by the final catalytic hydrogenation of the benzyl protecting groups in derivatives 22.

[1860-5397-16-152-i5]

Scheme 5: Reagents and conditions: a) 1. (BnO)2P-NMe2, 1H-tetrazole, 0 °C→rt, 1 h, 2. m-CPBA, 0 °C→rt, 1.5‒2 h; b) H2, 10% Pd/C, MeOH, rt, 3‒4 h; c) 3 M HCl, THF, 40 °C, 8 h.

Evaluation of the effects of the target compounds on the synthesis of lipid-linked galactan precursors

The availability of a series of compounds with different predicted affinities towards GlfT2 according to the docking studies (Table 1) encouraged us to evaluate them experimentally. The compounds were tested in an assay using an enzymatically active fraction of cell envelope from Mycobacterium smegmatis mc2155 and UDP-[14C]Galp as a tracer of the build-up of lipid-linked galactan precursors. The crude enzymes used in the assay allow for in situ synthesis of the acceptor for galactan polymerization, decaprenyl-P-P-GlcNAc-Rha (GL2), from endogenous decaprenyl phosphate and sugar nucleotides UDP-GlcNAc and TDP-Rha supplied in the reaction mixture. Conversion of UDP-[14C]Galp to UDP-[14C]Galf, which is required by mycobacterial galactofuranosyl transferases, was enabled by trace amounts of the cytosolic UDP-Galp mutase present in the prepared enzyme fraction. Consequently, this assay monitored not only GlfT2, but also activities of other enzymes employed in the synthesis of its acceptor substrate, decaprenyl-P-P-GlcNAc-Rha-Galf2 (GL4). Based on the previous experimental data, which established Km values for GlfT2 250 or 380 µM at 37 °C [10,18,19], we tested the whole set of target compounds at a concentration of 500 µM. TLC analysis of the lower galactan precursors, decaprenyl-P-P-GlcNAc-Rha-Galf1-3 (GL3-5) shows that under our experimental conditions none of the enzymes involved in the synthesis of lipid-linked galactan, including the galactofuranosyl transferases GlfT1 (produces GL3 and GL4) and GlfT2 (produces GL5), were affected (Figure 3).

[1860-5397-16-152-3]

Figure 3: TLC analysis of the effects of the target compounds at 500 μM on the production of lower lipid-linked galactan precursors. CON, control; GL3–5, glycolipids 3–5. GL3 and GL4 are products of GlfT1, GL5 is produced by GlfT2.

Despite the predicted low docking scores for the compounds 1bα, 2c, 3c and 1cβ, the quantification of the incorporation of the radioactive label to the more polymerized lipid-linked galactan precursors did not reveal inhibitory effects (Supporting Information File 1, Table S1). Nevertheless, our recent summary of the efforts towards identification of GlfT1 and GlfT2 inhibitors revealed that most compounds were reported to have effects on these enzymes at low mM concentrations (up to 8 mM) [6]. These molecules were designed as substrate mimics or transition state analogs and their IC50 values, ranging between 0.332–3.85 mM were established by a spectrophotometric assay with a purified recombinant GlfT2 enzyme, UDP-Galf as a donor substrate and analogs of the acceptor substrate [24-30]. The best reported IC50 value (0.180 mM) for a putative GlfT2 inhibitor was obtained for a fluorinated exo-glycal analogue of UDP-Galf in a radiometric assay with a glycolipid acceptor substrate and crude mycobacterial enzymes [31]. Therefore, we decided to examine compounds 1bα and 3a, representing the best and the worst inhibitors, respectively, according to the docking study, in the range of 1–10 mM. Interestingly, while compound 1bα showed a dose-dependent inhibitory effect in this experiment, compound 3a did not (Table 2). Although the crude system that we used in the enzymology experiments precludes precise kinetic characterization of the studied inhibitors on the target enzyme, GlfT2, it does have some advantages compared to the spectrophotometric GlfT2 assay [19]. Firstly, in the crude assay GlfT2 uses its natural acceptor, GL4, which could affect its activity and thus the outcome of the inhibition studies. Secondly, it allows to evaluate inhibition of the enzymes involved in GL4 production (WecA, WbbL, UGM, GlfT1; Supporting Information File 1, Figure S1) [6] by the tested compounds, which could indicate the unspecific effects. These cannot be precluded, especially if relatively high concentrations of the studied molecules are required for achieving substantial enzyme inhibition, which is the case for the reported GlfT2 inhibitors [6,24]. In our experiment TLC examination of the lower galactan precursors (GL3–5) points to subtle changes in their relative amounts in the presence of 1bα in the reaction mixture (Figure 4). In the control reaction, the major radiolabeled glycolipid species are GL4, the product of GlfT1 and GL5, the product of GlfT2. In the presence of 10 mM 1bα the predominant bands are GL3 and GL4, the products of GlfT1, which could point to a GlfT2 inhibition.

Table 2: Evaluation of the effects of compounds 1bα and 3a on the synthesis of the lipid-linked galactan polymera.

Compound Set1 (dpm) Set2 (dpm) Average % of inhibition
control 11,449 9,587 10,518 0
1 mM 1bα 10,041 11,384 10,712 −2
2 mM 1bα 9,912 8,937 9,425 10
5 mM 1bα 7,124 6,354 6,739 36
10 mM 1bα 3,051 2,887 2,969 72
1 mM 3a 8,602 8,638 8,620 18
2 mM 3a 8,580 6,498 7,539 28
5 mM 3a 6,054 6,029 6,041 43
10 mM 3a 7,631 7,063 7,347 30

aThe values in the table represent total incorporation of radioactive galactose from UDP-[14C]-Gal into the lipid-linked galactan polymer extracted by the solvents TT3 and E-soak (see Experimental part in Supporting Information File 1) in duplicate sets. The experiments were performed with 250 μg of the cell envelope protein in the reaction mixtures.

[1860-5397-16-152-4]

Figure 4: TLC analysis of the dose effects of the compounds 1bα and 3a on production of lower lipid-linked galactan precursors. CON, control; GL3–GL5, glycolipids 3–5. GL3 and GL4 are the products of GlfT1, GL5 is produced by GlfT2.

Conclusion

In summary, a small library of ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones 13 was synthesized. We performed docking studies of these compounds into the active site of GlfT2 by computational chemistry methods. Although the docking study showed good binding affinities of the prepared compounds towards the GlfT2 active site, their biological evaluation revealed a very poor effect on the synthesis of lipid-linked galactan. However, the experiments evaluating the dose effects of the predicted best (1bα) and the worst (3a) inhibitor on the mycobacterial galactan synthesis indicated the possibility of GlfT2 inhibition by 1bα at the highest tested concentration (10 mM). However, it is clear that given the high concentration of this compound required to achieve inhibitory effects on the galactan synthesis, this approach requires further optimization. Indeed, our pilot docking study was performed with compounds initially targeted at the transition state of GnT-I. Moreover, presented structures do not include the uridine mimicking part which can increase the binding affinity significantly. Attaching of this part is a topic of further synthesis and second-generation compound library preparation. We believe that our further efforts, both in synthetic and computational fields, will point to the potential of the in silico methods for the design of new GlfT2 inhibitors.

Supporting Information

The Supporting Information provides the biochemical pathway for the biosynthesis of mycobacterial galactan, superimposition of docked structures and evaluation of the effects of the target compounds on the synthesis of the lipid-linked galactan polymer. Complete experimental procedures, spectral characterization of all prepared compounds and copies of their 1H NMR and 13C NMR spectra are also provided.

Supporting Information File 1: Experimental and analytical data.
Format: PDF Size: 5.8 MB Download

Funding

The presented work was supported by the project VEGA 2/0024/16 and by the Slovak Research and Development Agency (APVV-15-0515). The research has also been financed by program SASPRO (ArIDARuM, 0005/01/02) - co-funded by the People Programme (Marie Curie Actions 7FP, grant agreement REA no. 609427) and co-financed by the Slovak Academy of Sciences (S.K.).

References

  1. Global TB Report 2019; WHO: Geneva, Switzerland, 2019. https://apps.who.int/iris/bitstream/handle/10665/329368/9789241565714-eng.pdf?ua=1 (accessed Feb 13, 2020).
    Return to citation in text: [1]
  2. Tiberi, S.; du Plessis, N.; Walzl, G.; Vjecha, M. J.; Rao, M.; Ntoumi, F.; Mfinanga, S.; Kapata, N.; Mwaba, P.; McHugh, T. D.; Ippolito, G.; Migliori, G. B.; Maeurer, M. J.; Zumla, A. Lancet Infect. Dis. 2018, 18, e183–e198. doi:10.1016/s1473-3099(18)30110-5
    Return to citation in text: [1]
  3. Daffé, M.; Marrakchi, H. Microbiol. Spectrum. 2019, 7, No. 4. doi:10.1128/microbiolspec.gpp3-0027-2018
    Return to citation in text: [1]
  4. Bhamidi, S.; Scherman, M. S.; Jones, V.; Crick, D. C.; Belisle, J. T.; Brennan, P. J.; McNeil, M. R. J. Biol. Chem. 2011, 286, 23168–23177. doi:10.1074/jbc.m110.210534
    Return to citation in text: [1]
  5. Richards, M. R.; Lowary, T. L. ChemBioChem 2009, 10, 1920–1938. doi:10.1002/cbic.200900208
    Return to citation in text: [1]
  6. Konyariková, Z.; Savková, K.; Kozmon, S.; Mikušová, K. Antibiotics (Basel, Switz.) 2020, 9, No. 20. doi:10.3390/antibiotics9010020
    Return to citation in text: [1] [2] [3] [4]
  7. Mikušová, K.; Mikuš, M.; Besra, G. S.; Hancock, I.; Brennan, P. J. J. Biol. Chem. 1996, 271, 7820–7828. doi:10.1074/jbc.271.13.7820
    Return to citation in text: [1]
  8. Beláňová, M.; Dianišková, P.; Brennan, P. J.; Completo, G. C.; Rose, N. L.; Lowary, T. L.; Mikušová, K. J. Bacteriol. 2008, 190, 1141–1145. doi:10.1128/jb.01326-07
    Return to citation in text: [1] [2]
  9. Weston, A.; Stern, R. J.; Lee, R. E.; Nassau, P. M.; Monsey, D.; Martin, S. L.; Scherman, M. S.; Besra, G. S.; Duncan, K.; McNeil, M. R. Tubercle Lung Dis. 1998, 78, 123–131. doi:10.1016/s0962-8479(98)80005-1
    Return to citation in text: [1]
  10. Wheatley, R. W.; Zheng, R. B.; Richards, M. R.; Lowary, T. L.; Ng, K. K. S. J. Biol. Chem. 2012, 287, 28132–28143. doi:10.1074/jbc.m112.347484
    Return to citation in text: [1] [2] [3] [4]
  11. Janoš, P.; Kozmon, S.; Tvaroška, I.; Koča, J. Chem. – Eur. J. 2018, 24, 7051–7059. doi:10.1002/chem.201800558
    Return to citation in text: [1] [2]
  12. Raab, M.; Kozmon, S.; Tvaroška, I. Carbohydr. Res. 2005, 340, 1051–1057. doi:10.1016/j.carres.2005.01.041
    Return to citation in text: [1]
  13. Varki, A.; Cummings, R. D.; Esko, J. D.; Freeze, H. H.; Stanley, P.; Bertozzi, C. R.; Hart, G. W.; Etzler, M. E. Essentials of Glycobiology, 2nd ed.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, NY, U.S.A., 2009.
    Return to citation in text: [1]
  14. Baráth, M.; Koóš, M.; Tvaroška, I.; Hirsch, J. Chem. Pap. 2015, 69, 339–347. doi:10.1515/chempap-2015-0017
    Return to citation in text: [1]
  15. Baráth, M.; Lin, C.-H.; Tvaroška, I.; Hirsch, J. Chem. Pap. 2015, 69, 348–357. doi:10.1515/chempap-2015-0063
    Return to citation in text: [1]
  16. Bella, M.; Yan, S.; Šesták, S.; Kozmon, S.; Lin, C.-H.; Mucha, J.; Koóš, M. Eur. J. Org. Chem. 2017, 6179–6191. doi:10.1002/ejoc.201701102
    Return to citation in text: [1] [2] [3] [4]
  17. Jakubčinová, J.; Kozmon, S.; Šesták, S.; Baráth, M. ChemistrySelect 2020, 5, 4967–4972. doi:10.1002/slct.202001098
    Return to citation in text: [1] [2] [3] [4]
  18. Poulin, M. B.; Zhou, R.; Lowary, T. L. Org. Biomol. Chem. 2012, 10, 4074–4087. doi:10.1039/c2ob25159k
    Return to citation in text: [1] [2] [3]
  19. Rose, N. L.; Zheng, R. B.; Pearcey, J.; Zhou, R.; Completo, G. C.; Lowary, T. L. Carbohydr. Res. 2008, 343, 2130–2139. doi:10.1016/j.carres.2008.03.023
    Return to citation in text: [1] [2] [3] [4]
  20. Tvaroška, I. Carbohydr. Res. 2015, 403, 38–47. doi:10.1016/j.carres.2014.06.017
    Return to citation in text: [1]
  21. Fletcher, H. G., Jr.; Diehl, H. W. Carbohydr. Res. 1971, 17, 383–391. doi:10.1016/s0008-6215(00)82546-x
    Return to citation in text: [1]
  22. Joseph, C. C.; Regeling, H.; Zwanenburg, B.; Chittenden, G. J. F. Carbohydr. Res. 2002, 337, 1083–1087. doi:10.1016/s0008-6215(02)00100-3
    Return to citation in text: [1]
  23. Elchert, B.; Li, J.; Wang, J.; Hui, Y.; Rai, R.; Ptak, R.; Ward, P.; Takemoto, J. Y.; Bensaci, M.; Chang, C.-W. T. J. Org. Chem. 2004, 69, 1513–1523. doi:10.1021/jo035290r
    Return to citation in text: [1]
  24. Vembaiyan, K.; Pearcey, J. A.; Bhasin, M.; Lowary, T. L.; Zou, W. Bioorg. Med. Chem. 2011, 19, 58–66. doi:10.1016/j.bmc.2010.11.044
    Return to citation in text: [1] [2]
  25. Lowary, T. L.; Li, J. Molecules 2016, 21, 1053. doi:10.3390/molecules21081053
    Return to citation in text: [1]
  26. Cocaud, C.; Maujoin, A.; Zheng, R. B.; Lowary, T. L.; Rodrigues, N.; Percina, N.; Chartier, A.; Buron, F.; Routier, S.; Nicolas, C.; Martin, O. R. Eur. J. Org. Chem. 2017, 6192–6201. doi:10.1002/ejoc.201701283
    Return to citation in text: [1]
  27. Cocaud, C.; Zheng, R. B.; Lowary, T. L.; Poisson, T.; Pannecoucke, X.; Nicolas, C.; Martin, O. R. Carbohydr. Res. 2018, 461, 45–50. doi:10.1016/j.carres.2018.03.009
    Return to citation in text: [1]
  28. Frigell, J.; Pearcey, J. A.; Lowary, T. L.; Cumpstey, I. Eur. J. Org. Chem. 2011, 1367–1375. doi:10.1002/ejoc.201001380
    Return to citation in text: [1]
  29. Li, J.; Lowary, T. L. Med. Chem. Commun. 2014, 5, 1130–1137. doi:10.1039/c4md00067f
    Return to citation in text: [1]
  30. Frédéric, C. J.-M.; Tikad, A.; Fu, J.; Pan, W.; Zheng, R. B.; Koizumi, A.; Xue, X.; Lowary, T. L.; Vincent, S. P. Chem. – Eur. J. 2016, 22, 15913–15920. doi:10.1002/chem.201603161
    Return to citation in text: [1]
  31. Dumitrescu, L.; Eppe, G.; Tikad, A.; Pan, W.; El Bkassiny, S.; Gurcha, S. S.; Ardá, A.; Jiménez-Barbero, J.; Besra, G. S.; Vincent, S. P. Chem. – Eur. J. 2014, 20, 15208–15215. doi:10.1002/chem.201404180
    Return to citation in text: [1]

Interesting articles

Sumudu P. Leelananda and Steffen Lindert

Tai Nguyen Van, Audrey Hospital, Corinne Lionne, Lars P. Jordheim, Charles Dumontet, Christian Périgaud, Laurent Chaloin and Suzanne Peyrottes

Rodrigo Abonia, Luisa F. Gutiérrez, Braulio Insuasty, Jairo Quiroga, Kenneth K. Laali, Chunqing Zhao, Gabriela L. Borosky, Samantha M. Horwitz and Scott D. Bunge

© 2020 Baráth et al.; licensee Beilstein-Institut.
This is an Open Access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0). Please note that the reuse, redistribution and reproduction in particular requires that the authors and source are credited.
The license is subject to the Beilstein Journal of Organic Chemistry terms and conditions: (https://www.beilstein-journals.org/bjoc)

 
Back to Article List