Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy

• María Sanromán Iglesias and
• Marek Grzelczak

Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20

• single-base changes associated with the breast cancer gene BRCA1. The detection limit was 0.26 nM, corresponding to 31.2 fmol of a target. Recently, advances in the development of DNA circuits based on hybridization and strand-exchange reactions have attracted much attention. Two examples of DNA circuits

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

Molecular architectonics of DNA for functional nanoarchitectures

• Debasis Ghosh,
• Lakshmi P. Datta and
• Thimmaiah Govindaraju

Beilstein J. Nanotechnol. 2020, 11, 124–140, doi:10.3762/bjnano.11.11

• the ECL quenching via formation of hydrogen peroxide. Kim and co-workers developed an innovative approach of intercalation of the anticancer drug doxorubicin within the DNA tetrahedron that showed improved therapeutic efficacy in drug-resistant breast cancer cells [70]. The doxorubicin-encapsulated

Advanced hybrid nanomaterials

• Andreas Taubert,
• Fabrice Leroux,
• Pierre Rabu and
• Verónica de Zea Bermudez

Beilstein J. Nanotechnol. 2019, 10, 2563–2567, doi:10.3762/bjnano.10.247

• is simultaneously employed as a contrast agent in magnetic resonance imaging (MRI) and for local heating therapy using magnetic particle hyperthermia [33]. In vitro hyperthermia tests showed efficiency in inoculating mouse breast cancer cells. Another study reports the use of alendronate-coated gold

Targeted therapeutic effect against the breast cancer cell line MCF-7 with a CuFe₂O₄/silica/cisplatin nanocomposite formulation

• B. Rabindran Jermy,
• Vijaya Ravinayagam,
• Widyan A. Alamoudi,
• Dana Almohazey,
• Hatim Dafalla,
• Lina Hussain Allehaibi,
• Abdulhadi Baykal,
• Muhammet S. Toprak and
• Thirunavukkarasu Somanathan

Beilstein J. Nanotechnol. 2019, 10, 2217–2228, doi:10.3762/bjnano.10.214

• /HYPS was applied as the support. An in vitro experiment was conducted to determine the potential of CuFe2O4/HYPS as an anticancer agent against the human breast cancer cell line MCF-7. The results show that the nanoparticle formulation can effectively target cancerous cells and could be an effective

• ), scanning electron microscopy (SEM) equipped with energy dispersive X-ray (EDX) spectroscopy and transmission electron microscopy (TEM) techniques. The study showed the high cisplatin release capability and targeted anticancer efficiency demonstrated in vitro in the breast cancer cell line MCF-7. Materials

• effectively reduce the viability of the human breast cancer cell line MCF7. Thus, cisplatin/CuFe2O4/HYPS nanoparticles can be considered an interesting option for drug delivery (Scheme 1). Conclusion An effective multifunctional CuFe2O4/HYPS nanocomposite system has been developed to deliver cisplatin

Review of advanced sensor devices employing nanoarchitectonics concepts

• Katsuhiko Ariga,
• Tatsuyuki Makita,
• Masato Ito,
• Taizo Mori,
• Shun Watanabe and
• Jun Takeya

Beilstein J. Nanotechnol. 2019, 10, 2014–2030, doi:10.3762/bjnano.10.198

• could be applicable for demands requiring the evaluation of small normal pressures even on dynamic surfaces such as natural tissues and is expected to be useful for in situ biomedical digital monitoring, such as palpation for breast cancer. Triboelectric nanogenerators to convert mechanical energy to

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• anticancer activities, such as restraining the hTERT gene expression in A549 lung cancer cells [3], inhibiting breast cancer stem-like cells via Wnt β-catenin signaling [4], impeding hepatocellular carcinoma cells by increasing DDX3 expression [5], and inducing apoptosis of prostate cancer cells through

• [30]. Phytosomes have been used as drug delivery systems of several insoluble natural drugs in recent years. Sinigrin [31] and epigallocatechin-3-O-gallate [32] loaded in phytosomes showed stronger antiproliferative activity than free drugs against melanoma cells and breast cancer cells. In this work

Tungsten disulfide-based nanocomposites for photothermal therapy

• Tzuriel Levin,
• Hagit Sade,
• Rina Ben-Shabbat Binyamini,
• Maayan Pour,
• Iftach Nachman and
• Jean-Paul Lellouche

Beilstein J. Nanotechnol. 2019, 10, 811–822, doi:10.3762/bjnano.10.81

• (cervical cancer) and MCF7 cells (breast cancer), in higher percentages compared to non-functionalized nanotubes. In addition, WS2-CAN-mag nanocomposites with two types of organic polymers were successfully prepared and characterized. Indeed, functionalization with CAN-mag gives WS2 nanotubes the added

• human cancer cells – HeLa (cervical cancer) and MCF7 (breast cancer). The cells were cultured on 24-well plates. When the cells reached 80% confluence, freshly prepared aqueous dispersions of WS2-NT or WS2-NT-CM (45 µL, 1 mg/mL) were added to two of the plates, and a third plate, with no additives, was

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• against specific cell populations. As example, Herceptin is an antibody that recognizes the human epidermal growth factor receptor 2 (HER2) overexpressed in breast cancer cells (HER2+). This antibody has been attached on the surface of poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with the

Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots

• Siyi Hu,
• Yu Ren,
• Yue Wang,
• Jinhua Li,
• Junle Qu,
• Liwei Liu,
• Hanbin Ma and
• Yuguo Tang

Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3

• as an optical process for MCF-7 breast cancer cells. Keywords: bioimaging; gold nanorods; photoluminescence enhancement; quantum dots; Introduction In the past decades, quantum dots (QDs) have proven to be increasingly useful for their unique features [1][2][3][4][5]. The light emission from QDs

• @CdSe/ZnS can be used in cell imaging studies, after conjugation with FA, we performed a MCF-7 breast cancer cell targeting imaging study, and the results as shown in Figure 8. Robust cellular uptake could be obtained from the CdSe/ZnS@FA and GNR@CdSe/ZnS@FA treated samples. The obvious luminescence and

• staining appearing in Figure 8 was due to the accumulation of functionalized nanoparticles in the cells, and there was no sign of any damage to the cell, demonstrating passive uptake in MCF-7 breast cancer cells using CdSe/ZnS@FA and GNR@CdSe/ZnS@FA. However, because the PL intensity and cell morphology of

Size-selected Fe₃O₄–Au hybrid nanoparticles for improved magnetism-based theranostics

• Maria V. Efremova,
• Yulia A. Nalench,
• Eirini Myrovali,
• Anastasiia S. Garanina,
• Ivan S. Grebennikov,
• Polina K. Gifer,
• Maxim A. Abakumov,
• Marina Spasova,
• Makis Angelakeris,
• Alexander G. Savchenko,
• Michael Farle,
• Natalia L. Klyachko,
• Alexander G. Majouga and
• Ulf Wiedwald

Beilstein J. Nanotechnol. 2018, 9, 2684–2699, doi:10.3762/bjnano.9.251

• extraordinarily high r2-relaxivity of 495 mM−1·s−1 along with a specific loss power of 617 W·gFe−1 and 327 W·gFe−1 for hyperthermia in aqueous and agarose systems, respectively. The functional in vitro hyperthermia test for the 4T1 mouse breast cancer cell line demonstrated 80% and 100% cell death for immediate

• diameter from 6 to 25 nm. The 25 nm and 44 nm diameter NPs show similar theranostic performance. In in vitro experiments we detected the death of 4T1 mouse breast cancer cells at a rate of 79 ± 8% after exposure to 25 nm Fe3O4–Au hybrids for 30 min in an ac magnetic field (AMF) with 261–393 kHz and 25 mT

• mouse breast cancer cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). They were cultured in RPMI-1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 2 mM L-glutamine (Gibco) at 37 °C in a humidified incubator supplied with 5% CO2. MTS

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• instance, piperine, a major chemical compound present in black pepper, has shown anti-inflammatory, anti-angiogenic, and anti-arthritic effects [15]. Moreover, it has been reported that black pepper is capable to reduce breast cancer cell proliferation [16][17][18]. Turmeric is an abundant medicinal herb

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• metastatic breast cancer cell line MDA-MD-231 and primary breast cancer cell line MCF7 to explore the transfer of quantum dots (QDs) to cancer cells. First, the optimal conditions for high-content QD loading in MSCs were established. Then, QD uptake in breast cancer cells was assessed after 24 h in a 3D co

• -culture with nanoengineered MSCs. We found that incubation of MSCs with QDs in a serum-free medium provided the best accumulation results. It was found that 24 h post-labelling QDs were eliminated from MSCs. Our results demonstrate that breast cancer cells efficiently uptake QDs that are released from

• nanoengineered MSCs in a 3D co-culture. Moreover, the uptake is considerably enhanced in metastatic MDA-MB-231 cells compared with MCF7 primary breast cancer cells. Our findings suggest that nanoengineered MSCs could serve as a vehicle for targeted drug delivery to metastatic cancer. Keywords: cancer

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• , Tween 80-coated core–shell nanoparticles presented enhanced optical and MR signal intensity, good colloidal stability, low cytotoxicity and nonspecific internalization into two different breast cancer cell lines, which indicates that these nanoparticles could be applied as an efficient, dual-modal

• (UC) emission intensity of Tween 80-coated nanoparticles in comparison with oleic acid coated UCNPs. In addition, the nonspecific uptake and distribution of non-targeted Tween 80-coated UCNPs in human MCF-7 and MDB-MA-231 breast cancer cells was visualized by using confocal fluorescence microscopy

• were studied to evaluate their application to biological imaging using MDA-MB-231 breast cancer cells. The confocal image of MDA-MB-231 breast cancer cells after 24 h incubation with UCNPs is shown in Figure 6A. The scatter of excitation light by intracellular cell structures was marked with red color

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• -cytotoxic against L929 mouse fibroblast cell line. In addition, paclitaxel-loaded nanoparticles have a significant anticancer effect against MCF-7 human breast cancer cell line as compared with a paclitaxel solution in DMSO. Conclusion: According to the results of this study, both amphiphilic cyclodextrin

• of chemotherapy with PCX. A promising step was taken with the FDA approval of albumin nanoparticle bound PCX (Abraxane®) in 2005 for breast cancer treatment with a significantly lower dose [8]. This was considered a breakthrough in PCX formulation development as it avoided the use of solubilizers

• their drug encapsulation, release profile and anticancer activity on MCF-7 human breast cancer cell line in particular. Safety and apoptotic efficacy of blank and PCX-loaded cationic or anionic amphiphilic CD nanoparticles were evaluated with cell culture studies against a series of healthy and cancer

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• . There were successful attempts to use a similar quantum dot–chlorin e6 complex in photodynamic cancer therapy [15]. Another study has shown that QDs, conjugated with antibodies against CD44, a marker of cancer stem-like cells, can be selectively engulfed by breast cancer cells [16]. Such surface

• therapeutic nano-agents [24]. Studies on melanoma [25], prostate cancer [26], breast cancer [6] and lung cancer [27] have shown the ability of MSCs to home to cancer sites in vivo. In the tumour microenvironment, MSCs play a role in the formation of the tumour stroma and support cancer metastasis [28

• propose that cancer cell and MSC co-culture model could be used to demonstrate the applicability of QD-labelled MSCs for cancer theranostics. For example, Pietila et al. have demonstrated that direct cell–cell contact is required for QD–mortalin antibody transfer from nano-engineered MSCs to the breast

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• male patient with well differentiated hepatocellular carcinoma and SK-Hep1 received from ascetic fluid of a patient with adenocarcinoma of the liver), one breast cancer cell line (MCF-7 obtained from a female patient) and one human pancreatic ductal adenocarcinoma cell line (Capan-1). A protocol was

• RPMI 1640 liquid medium with 20 mM HEPES, stable glutamine (FG 1235, Biochrom AG, Germany), 10% fetal bovine serum (10099-133, Life Technologies, Germany) together with 100 units/mL of penicillin and 100 µg/mL of streptomycin (15140, Gibco®, Life Technologies GmbH, Germany). Cultivation of MCF-7 breast

• cancer cells was performed in RPMI 1640 with 2.0 g/L NaHCO3 (F 1215, Biochrom AG, Germany) and 40 mg/L folic acid (F7876, Sigma–Aldrich, Germany) with the same amount of fetal bovine serum, penicillin and streptomycin as described above for liver cells. The pancreatic cancer cell line Capan-1 was

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• of prostate cancer [19]. AR contributes to breast cancer progression and development [20] and its mutation is involved in the progression of neurodegenerative diseases, such as the X-linked spinal and bulbar muscular atrophy [21]. In order to better understand and characterize the behavior and

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• literature, as published by Ambrosia and co-workers in studying the effect of AuNP complexes in enhanced ELISA for the detection of breast cancer biomarkers [12]. In this work, the authors assessed three different concentrations of AuNP probes (ranging approximately from 1.4 to 0.014 AU) discarding the

Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling

• Nadezhda M. Zholobak,
• Anton L. Popov,
• Alexander B. Shcherbakov,
• Nelly R. Popova,
• Mykhailo M. Guzyk,
• Valeriy P. Antonovich,
• Alla V. Yegorova,
• Yuliya V. Scrypynets,
• Inna I. Leonenko,
• Alexander Ye. Baranchikov and
• Vladimir K. Ivanov

Beilstein J. Nanotechnol. 2016, 7, 1905–1917, doi:10.3762/bjnano.7.182

• -fixed ST-cells to be obtained (Supporting Information File 1, Figure S21). The findings suggest the possibility of the use of O-dots for the visual differentiation of normal cells and cells in a state of oxidative stress, as well as actively metabolizing cells. Non-fixed malignant breast cancer cells

• centrifuged and rinsed three times with EDTA, and then three times with pure water. Cell cultures The effects of O-dots on living cells were studied using reference diploid epithelial swine testicular cell line (ST-cells), from the collection of the Institute of Veterinary Medicine, UAAS, and malignant breast

• cancer cells (MCF-7S), from the collection of the Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NASU. We used a one-day culture of cells that were grown in a DMEM + RPMI medium (Sigma, USA) containing 7% FBS (fetal bovine serum; Sigma, USA), in the presence of kanamycin and

Comparison of the interactions of daunorubicin in a free form and attached to single-walled carbon nanotubes with model lipid membranes

• Dorota Matyszewska

Beilstein J. Nanotechnol. 2016, 7, 524–532, doi:10.3762/bjnano.7.46

• in the treatment of various types of cancer including leukemia, breast cancer, ovarian cancer, lung carcinoma and several sarcomas (Figure 1). Its mode of action consists in the intercalation into DNA double strand, which leads to the inhibition of the process of duplication and transcription of mRNA

Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection

• Dinusha N. Udukala,
• Hongwang Wang,
• Sebastian O. Wendel,
• Aruni P. Malalasekera,
• Thilani N. Samarakoon,
• Asanka S. Yapa,
• Gayani Abayaweera,
• Matthew T. Basel,
• Pamela Maynez,
• Raquel Ortega,
• Yubisela Toledo,
• Leonie Bossmann,
• Colette Robinson,
• Katharine E. Janik,
• Olga B. Koper,
• Ping Li,
• Massoud Motamedi,
• Daniel A. Higgins,
• Gary Gadbury,
• Gaohong Zhu,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2016, 7, 364–373, doi:10.3762/bjnano.7.33

• the central nanoparticle by means of a protease-cleavable consensus sequence and a second dye (Cy 5.5) that is directly linked. Based on the protease activities of urokinase plasminogen activator (uPA), MMPs 1, 2, 3, 7, 9, and 13, as well as CTS B and L, human breast cancer can be detected at stage I

• by means of a simple serum test. By monitoring CTS B and L stage 0 detection may be achieved. This initial study, comprised of 46 breast cancer patients and 20 apparently healthy human subjects, demonstrates the feasibility of protease-activity-based liquid biopsies for early cancer diagnosis

• . Keywords: biophotonics; breast cancer; iron/iron oxide nanoparticle; liquid biopsy; nanodiagnostics detection; nanomedicine; sub-femtomolar limit of detection; Introduction We have detected stage I breast cancer in human patients with statistical significance by means of a simple serum test using highly

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• . prepared a nanocomposite consisting of reduced graphene oxide hybridized with electrochemically co-reduced gold nanoparticles and ferrocene as a sensitive immunosensor of breast cancer biomarkers [14]. Very recently, Mars et al. showed that the aggregation of gold nanoparticles through a ferrocene-based

PLGA nanoparticles as a platform for vitamin D-based cancer therapy

• Maria J. Ramalho,
• Joana A. Loureiro,
• Bárbara Gomes,
• Manuela F. Frasco,
• Manuel A. N. Coelho and
• M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

• inhibition when compared to free calcidiol, and the PLA NPs enhanced the intracellular delivery of vitamin in breast cancer cells [14]. In another work, Bonor et al. [23] developed calcitriol-conjugated quantum dots to analyze calcitriol distribution and dynamics in mouse myoblast cells. The authors

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• liver cancer cells, NIH-3T3 mouse fibroblast cells, and 4T1 mouse breast cancer cells. Confocal laser scanning microscopy (CLSM) images indicated a successful bioconjugation of silica-coated QDs and MQDs with a bio-anchored membrane. Again, Salgueiriño-Maceira et al. [44] reported a new class of

• hypointense spot with excellent contrast against the surrounding tissues. Again, Corsi et al. [58] reported the synthesis of a modified hybrid nanocomposite material consisting of magnetic fluorescent NPs for bimodal diagnosis of breast cancer cells. The magnetic Fe3O4 NPs were prepared by using a co