Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• Zdenek Plichta Yulia Kozak Rostyslav Panchuk Viktoria Sokolova Matthias Epple Lesya Kobylinska Pavla Jendelova Daniel Horak Institute of Macromolecular Chemistry CAS, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• structure, 5-FU enables the misincorporation of the fluoro-nucleotides into RNA and DNA and effectively inhibits synthetic enzyme (i.e., thymidylate) synthase, which further results in the arrest of cellular growth and proliferation. However, due to the increased rate of metabolism in the blood, 5-FU has a

Nanocellulose: Recent advances and its prospects in environmental remediation

• Katrina Pui Yee Shak,
• Yean Ling Pang and
• Shee Keat Mah

Beilstein J. Nanotechnol. 2018, 9, 2479–2498, doi:10.3762/bjnano.9.232

Fabrication of photothermally active poly(vinyl alcohol) films with gold nanostars for antibacterial applications

• Mykola Borzenkov,
• Maria Moros,
• Claudia Tortiglione,
• Serena Bertoldi,
• Nicola Contessi,
• Silvia Faré,
• Angelo Taglietti,
• Agnese D’Agostino,
• Piersandro Pallavicini,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2018, 9, 2040–2048, doi:10.3762/bjnano.9.193

• local temperature increase was enough to inhibit bacterial growth viability and proliferation. Further studies will be performed whereby both the laser intensity and irradiation period are tuned, leading to improved antibacterial efficiency of the prepared films. Conclusion In the present study PVA

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• . Cytotoxicity studies were performed by using MTT assay, methylene-blue staining and SEM fixation and showed very good cell adhesion and proliferation, indicating the cytocompatibility of these fibrous scaffolds. Drug-release kinetics was measured for the evaluation of a controllable and sustained release of

• three-dimensional (3D) polymeric scaffolds acting as substrates for the growth, differentiation and proliferation of biological cells for their successful implementation in medical devices. This has led to the development of a new generation of diagnostic and therapeutic approaches [12][13]. Although

• degradation pattern of which is appropriate for this application, contributed positively to the anti-inflammatory action of dexamethasone and not to its immunosuppressive action. Cytocompatibility behavior The MTT assay shows the quantitative analysis of cell proliferation as a function of the time. The MTT

A visible-light-controlled platform for prolonged drug release based on Ag-doped TiO₂ nanotubes with a hydrophobic layer

• Caihong Liang,
• Jiang Wen and
• Xiaoming Liao

Beilstein J. Nanotechnol. 2018, 9, 1793–1801, doi:10.3762/bjnano.9.170

• essential to human being, previously published work [26] showed that Zn can greatly improve cell functionality by enhancing osteoblast proliferation and mineralization and promoting mesenchymal stem cell osteogenic differentiation. Meanwhile, its anti-inflammatory properties have also drawn wide attention

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• showed that the number of surface-attached cells increased with increasing patterning of the gelatin surface. Unlike the cell attachment assay, the results of a cell proliferation assay showed that Saos-2 cells prefer grooves with diameters of approximately 2 µm and 1 µm and pillars with diameters of 1

• µm and heights of 500 nm. The number of cells on pillars with heights of 2 µm was larger than those of the other gelatin surface patterns tested. Conclusion: These data support that a detailed design of the gelatin surface pattern can control both cell attachment and proliferation of Saos-2 cells

• . Thus, gelatin surfaces patterned using genipin crosslinking are now an available option for biocompatible material patterning. Keywords: cell attachment; cell proliferation; dental implants; gelatin; genipin; nanopatterning; Introduction Topography on the micro- and nanoscale is an important property

Bioinspired self-healing materials: lessons from nature

• Joseph C. Cremaldi and
• Bharat Bhushan

Beilstein J. Nanotechnol. 2018, 9, 907–935, doi:10.3762/bjnano.9.85

• mainly rely on high reproductive (cell division) rates and short life spans (fast evolution) for proliferation and survival. This review focuses on the multicellular plant and animal organisms, whose healing goal is homeostasis, or the body’s attempt to maintain itself. Homeostasis is defined as a

• the body. Inflammation initiates the innate immune response, often seen as a swelling redness as an increase in cells occurs at the site of the wound. In proliferation, fibroblasts (counterparts to the osteoblasts in hard tissue) secrete extracellular matrix, fibrinogen, and collagen to remodel tissue

Noble metal-modified titania with visible-light activity for the decomposition of microorganisms

• Maya Endo,
• Zhishun Wei,
• Kunlei Wang,
• Baris Karabiyik,
• Kenta Yoshiiri,
• Paulina Rokicka,
• Bunsho Ohtani,
• Agata Markowska-Szczupak and
• Ewa Kowalska

Beilstein J. Nanotechnol. 2018, 9, 829–841, doi:10.3762/bjnano.9.77

• albicans (C. albicans)) activity under visible-light irradiation and in the dark using disk diffusion, suspension, colony growth (“poisoned food”) and sporulation methods. It was found that silver-modified titania, besides remarkably high antibacterial activity (inhibition of bacterial proliferation

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• instance, piperine, a major chemical compound present in black pepper, has shown anti-inflammatory, anti-angiogenic, and anti-arthritic effects [15]. Moreover, it has been reported that black pepper is capable to reduce breast cancer cell proliferation [16][17][18]. Turmeric is an abundant medicinal herb

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• [14][15][16]. Moreover, in vitro 3D cultures could fill the gap between 2D in vitro testing and in vivo animal models [17][18]. Spheroid-based 3D models are widely used to test cancer cell growth/proliferation, invasion, angiogenesis, and immune interactions and have been employed in drug screening

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• alignment of ECM fibers is known to control the cell shape and motion [53]. During development, growth, and proliferation, the 2D/3D topography of ECM affects the cell spreading, focal adhesion strength, and intercellular mechanical signaling [54]. For example, the guided migration of cells in the ECM was

• architectures of hydrated dense collagen matrices allowed for the penetration of human dermal fibroblast from the surface into the matrix through the expression of metalloproteinases (collagenase I and gelatinase A), in which long-term migration and high-yield proliferation of cells were achieved, comparable to

• purposes, LCEs need to undergo additional physicochemical modifications. For example, Abbott and co-workers developed a cell-culture monitoring tool with which the orientational order of ECM-decorated nematic LCE substrates could be utilized for sensing ECM-attachment and proliferation of human embryonic

Humidity-dependent wound sealing in succulent leaves of Delosperma cooperi – An adaptation to seasonal drought stress

• Olga Speck,
• Mark Schlechtendahl,
• Florian Borm,
• Tim Kampowski and
• Thomas Speck

Beilstein J. Nanotechnol. 2018, 9, 175–186, doi:10.3762/bjnano.9.20

• , reversible cross-linking) are involved, a transfer to technology is especially promising. However, if biological mechanisms such as metabolism-driven modifications (e.g., biosynthesis, cell proliferation) are present, the technical application is much more difficult. A clear distinction must also be made

• mechanically driven mechanism. Subsequent to the relatively fast initial self-sealing processes, self-healing including biosynthesis and cell proliferation starts, and hence the formation of a specific wound healing tissue. After a few weeks, self-healing is completed showing pronounced healed tissue in the

Tailoring the nanoscale morphology of HKUST-1 thin films via codeposition and seeded growth

• Landon J. Brower,
• Lauren K. Gentry,
• Amanda L. Napier and
• Mary E. Anderson

Beilstein J. Nanotechnol. 2017, 8, 2307–2314, doi:10.3762/bjnano.8.230

• 25 °C samples. The 1.5 h sample at 25 °C had the highest thickness and roughness values. The 25 °C sample submerged for 5 h had a marked decrease in thickness and was the initial time point at which the proliferation of small particles was observed. In addition, durations of 1.5 and 5 h were

Bi-layer sandwich film for antibacterial catheters

• Gerhard Franz,
• Florian Schamberger,
• Hamideh Heidari Zare,
• Sara Felicitas Bröskamp and
• Dieter Jocham

Beilstein J. Nanotechnol. 2017, 8, 1982–2001, doi:10.3762/bjnano.8.199

• against solutions with Ag+ ions releasing catheters, the raising absorption is caused by exponential bacteria growth, which eventually becomes saturated. With this method, however, a decision is not possible whether the bacteria are already dead or still alive but have lost their ability for proliferation

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• changes in the cells and tissues. Some of these events include cell proliferation and apoptosis [1], ion transport [2] and other cellular process and diseases such as cancer [3][4][5], Parkinson's, and Alzheimer's disease [6]. Despite the large number of nanotechnology platforms available to date for

• (Figure 8B,C), a progressive accumulation of fluo-CNOs was observed during the cell proliferation. Additionally, the efficient uptake was supported by a three dimensional reconstruction of cells treated with CNOs (Figure 9). Finally, we demonstrated that the on/off fluorescent emission properties of the

• a positive control for cytotoxicity, the cells were incubated with 5% DMSO. Cell viability was determined using the cell proliferation reagent WST-1 (Roche Applied Sciences). After aspiration of the culture medium, a mixture of DMEM and WST1 reagent (1/10 volume) was added to each well. After 2 h of

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• characterized by loss of layered tissue architecture and an increase in tissue microstructural disorder in OCT images (Figure 12 b,c). The stromal layer demonstrates columnar proliferation towards the surface of tissue in the OCT image of a CIN 3 lesion (Figure 12b). The invasive carcinoma manifests itself in

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• compared with the ND-free living control (i.e., cells grown in polystyrene wells in a medium without diamond nanoparticles). Similar results were also obtained in a study by Vaijayanthimala et al. [11], in which the proliferation of HeLa cells and 3T3-L1 pre-adipocytes exhibited no significant difference

Surface functionalization of 3D-printed plastics via initiated chemical vapor deposition

• Christine Cheng and
• Malancha Gupta

Beilstein J. Nanotechnol. 2017, 8, 1629–1636, doi:10.3762/bjnano.8.162

• . Hong et al. demonstrated that simply dipping polycaprolactone/poly(lactic-co-glycolic acid) 3D scaffolds in mussel adhesive proteins promoted cellular adhesion, proliferation and differentiation, showing that a facile surface modification improved the viability of using 3D-printed scaffolds for tissue

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• human breast cancer cell line. For this purpose, MCF-7 cells were incubated with different concentrations of PCX in dimethyl sulfoxide (DMSO). Non-treated cells were incubated with DMEM alone and were used as control group. Cell proliferation was determined and the IC50 value of PCX was calculated and

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• an intracranial U87 glioma model. They demonstrated that active targeted and cationic core–shell nanoparticles could be effective in inhibition of tumor proliferation with higher accumulation in tumor area when they are administered intravenously [67]. Different studies also showed that nanoparticles

Micro- and nano-surface structures based on vapor-deposited polymers

• Hsien-Yeh Chen

Beilstein J. Nanotechnol. 2017, 8, 1366–1374, doi:10.3762/bjnano.8.138

• proliferation (FGF-2) and osteogenic differentiation (BMP-2) for adipose-derived stem cells [76]. Selective deposition The aforementioned methods rely on physical means to obtain spatially controlled surface modifications and patterned structures. A simpler approach is the selective inhibition of the vapor

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• combination with the carbon nanomaterials. The impact of the monotreatments and the combinatory treatments on cellular function was then systematically analyzed by using different experimental approaches (viability, short-term and long-term proliferation, cell death rate). DTX and MMC alone reduced the

• contrast, the dose of platinum-based chemotherapeutics could only be reduced by up to 3-fold by combination with carbon nanomaterials. Furthermore, combinatory treatments with CNFs led mostly to an additive inhibition of short- and long-term proliferation compared to the individual treatments. Also, higher

• proliferation as well as by an increased induction of apoptosis [27][28]. In order to further verify this concept and to avoid a bias that might be caused by the structurally similar platinum-based chemotherapeutics, we investigated other chemotherapeutics relevant for urological cancers, namely DTX and

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• in skin MSCs was clathrin-mediated endocytosis. QDs were mainly localized in early endosomes after 6 h as well as in late endosomes and lysosomes after 24 h. QDs in concentrations ranging from 0.5 to 64 nM had no effect on cell viability and proliferation. The expression of MSC markers, CD73 and CD90

• affect the osteogenesis and chondrogenesis capacities of bone marrow MSCs [17]. The impact of QD labelling on the biological properties of targeted stem cells, such as proliferation, cell cycle, and apoptosis, remains elusive. Therefore, further research on MSCs with regard to the delivery of QDs for

• daughter cells (Figure 3c). Inhibition of proliferation was additionally confirmed by analysing the cell number in the respective medium (data not shown). The addition of QDs did not change the expression of Ki67 (data not shown). QD effect on immunophenotype, proliferation and differentiation of MSCs The

Recombinant DNA technology and click chemistry: a powerful combination for generating a hybrid elastin-like-statherin hydrogel to control calcium phosphate mineralization

• Mohamed Hamed Misbah,
• Mercedes Santos,
• Luis Quintanilla,
• Christina Günter,
• Matilde Alonso,
• Andreas Taubert and
• José Carlos Rodríguez-Cabello

Beilstein J. Nanotechnol. 2017, 8, 772–783, doi:10.3762/bjnano.8.80

• specific surface area and three-dimensional porous structures. In addition, osteoblast proliferation and apoptosis are affected by the size and shape of CP. Despite this interest, control of the formation of CP nanostructures with specific characteristics remains a challenge. Although dry methods and high

• , ELR membranes with SNA15 epitopes and/or surface topographies have been conducted on the cell morphology, adhesion, proliferation and differentiation, and their potential for dental and orthopedic implants integration during the mineralization process. Although the mineralization activity of ELRs