Microwave synthesis of high-quality and uniform 4 nm ZnFe₂O₄ nanocrystals for application in energy storage and nanomagnetics

• Christian Suchomski,
• Ben Breitung,
• Ralf Witte,
• Michael Knapp,
• Sondes Bauer,
• Tilo Baumbach,
• Christian Reitz and
• Torsten Brezesinski

Beilstein J. Nanotechnol. 2016, 7, 1350–1360, doi:10.3762/bjnano.7.126

• in a Rh matrix. The center shifts are quoted relative to α-Fe foil at room temperature. The spectra were analyzed using the WinNORMOS software [24]. Transmission electron microscopy was performed on a Tecnai G2-F20ST microscope (FEI) operated at 200 keV. The bright-field images were analyzed using

• shape of the ZFO nanoparticles was investigated by means of transmission electron microscopy (TEM). The low-magnification bright-field TEM image in Figure 1a shows that they are spherical in shape with a narrow size distribution around 4 nm. Both high-resolution TEM (HRTEM, Figure 1b) and selected-area

• conclude that the particles are of good quality and thus hold promise for application in various fields of nanotechnology. Electron microscopy of as-prepared ZFO nanoparticles. (a) Bright-field TEM image. (b) HRTEM image and (c) SAED pattern demonstrating the crystallinity. Note that only the most intense

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

• Claudia Messerschmidt,
• Daniel Hofmann,
• Anja Kroeger,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

• . The particle diameters specified by the manufacturer were 7 nm, 12 nm and 22 nm and will be referred to as SiNP-7, SiNP-12 and SiNP-22, respectively. Figure 1 shows the measured size distributions and representative TEM bright field micrographs of the respective SiNPs. Table 1 gives the average radius

• tridiem image filter (Gatan Inc., USA) and an energy dispersive X-ray fluorescence (EDX) detector (EDAX Inc., USA) for analytical element measurements. Conventional bright field images were acquired using a Gatan US1000 slow scan CCD camera (Gatan Inc., USA). Inelastic dark field imaging for NP

• identification Since the contrast of the silica particles in bright field imaging was too low for unambiguous identification, we applied inelastic dark field imaging techniques for the visualization of silica nanoparticles. Inelastic dark field imaging was conducted using the image filter/electron energy loss

Straightforward and robust synthesis of monodisperse surface-functionalized gold nanoclusters

• Silvia Varela-Aramburu,
• Richard Wirth,
• Chian-Hui Lai,
• Guillermo Orts-Gil and
• Peter H. Seeberger

Beilstein J. Nanotechnol. 2016, 7, 1278–1283, doi:10.3762/bjnano.7.118

• bright-field image of Glc-NCs showing monodisperse nanoclusters, scale bar 2 nm; D) size distribution of 110 nanoclusters of Glc-NCs yielding diameters of 2.02 ± 0.18 nm. A) Functionalization of Glc-NCs by coupling trifluoroethanol to the carboxylic acid groups on the surface of the nanocluster to yield

Functional diversity of resilin in Arthropoda

• Jan Michels,
• Esther Appel and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2016, 7, 1241–1259, doi:10.3762/bjnano.7.115

Manufacturing and investigation of physical properties of polyacrylonitrile nanofibre composites with SiO₂, TiO₂ and Bi₂O₃ nanoparticles

• Tomasz Tański,
• Wiktor Matysiak and
• Barbara Hajduk

Beilstein J. Nanotechnol. 2016, 7, 1141–1155, doi:10.3762/bjnano.7.106

• taken in bright field and dark field mode and using an HAADF detector. The results of the diffraction studies, obtained using analytical electron microscopy in nanoareas in the STEM mode, confirmed the phase composition and the crystalline structure from the earlier X-ray study. The diffraction pattern

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• surface of the carbon film. The particles were dried at room temperature for more than 1 h, and TEM micrographs were obtained at an accelerating voltage of 120 kV by Tecnai Spirit G2 (FEI, Brno, Czech Republic). Bright field imaging (BF) and selected area electron diffraction (SAED) were used to visualize

• counterstained with 0.1% Nuclear Fast Red (Sigma-Aldrich) for 1 min, mounted with HistoMount (Invitrogen) and covered using coverslip. After drying, the cells were analyzed under bright field using light microscope (ECLIPSE E200, Nikon Instruments, Japan). MTT cell viability assay After NSC labeling MTT (methyl

Templated green synthesis of plasmonic silver nanoparticles in onion epidermal cells suitable for surface-enhanced Raman and hyper-Raman scattering

• Marta Espina Palanco,
• Klaus Bo Mogensen,
• Marina Gühlke,
• Zsuzsanna Heiner,
• Janina Kneipp and
• Katrin Kneipp

Beilstein J. Nanotechnol. 2016, 7, 834–840, doi:10.3762/bjnano.7.75

• high non-linearity makes SEHRS a very sensitive method to probe spatial variations in local fields and to localize plasmonic nanostructures, surpassing also SERS. Here we compare SEHRS images and bright field microscopy of the onion cell layers. Additionally, our SERS and SEHRS experiments give

• bright field picture of the onion layer. The image displays the 1175 cm−1 SEHRS band (see spectrum in panel b). The color code represents the signal from lowest (blue) to highest (red). Acknowledgements We thank Harald Kneipp for support of the experiments and useful discussion. MG, ZH and JK

High-resolution noncontact AFM and Kelvin probe force microscopy investigations of self-assembled photovoltaic donor–acceptor dyads

• Benjamin Grévin,
• Pierre-Olivier Schwartz,
• Laure Biniek,
• Martin Brinkmann,
• Nicolas Leclerc,
• Elena Zaborova and
• Stéphane Méry

Beilstein J. Nanotechnol. 2016, 7, 799–808, doi:10.3762/bjnano.7.71

• subsequent recovery on TEM copper grids. TEM was performed in bright field, high-resolution and diffraction modes using a CM12 Philips microscope equipped with a MVIII (Soft Imaging System) CCD camera. In order to avoid beam damage to the thin films, after focusing and correction of astigmatism, the electron

• domains. The first hypothesis is particularly supported by the existence of cross-hatch patterns in the TEM images (Figure 6b), clearly revealing that lamellae with different π-stacking directions overlap in some parts of the film. The TEM bright field images correspond to 2D projections of a 3D film

• , AVib = 14 nm). (b,c) nc-AFM topographic (b) and damping (c) images (1000 × 1000 nm) of the AD3 film on ITO/PEDOT:PSS (Δf = −20 Hz, AVib = 20 nm). (d) Bright field TEM image of an AD3 film. The area corresponding to some flat-on lamellae are highlighted by black contours in (a–d). (a) 300 × 300 nm nc

Assembling semiconducting molecules by covalent attachment to a lamellar crystalline polymer substrate

• Rainhard Machatschek,
• Patrick Ortmann,
• Renate Reiter,
• Stefan Mecking and
• Günter Reiter

Beilstein J. Nanotechnol. 2016, 7, 784–798, doi:10.3762/bjnano.7.70

Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles

• Dulce G. Romero-Urbina,
• Humberto H. Lara,
• J. Jesús Velázquez-Salazar,
• M. Josefina Arellano-Jiménez,
• Eduardo Larios,
• Anand Srinivasan,
• Jose L. Lopez-Ribot and
• Miguel José Yacamán

Beilstein J. Nanotechnol. 2015, 6, 2396–2405, doi:10.3762/bjnano.6.246

• emission transmission electron microscope operated at 200 kV. HAADF-STEM bacterial cell images were obtained using Cs-corrected JEOL JEM-ARM-200F microscope in both bright-field (BF) and dark-field (DF) modes. The microscope was operated at 200 kV using a convergence angle of 26 mrad and collection semi

Silica-coated upconversion lanthanide nanoparticles: The effect of crystal design on morphology, structure and optical properties

• Uliana Kostiv,
• Miroslav Šlouf,
• Hana Macková,
• Alexander Zhigunov,
• Hana Engstová,
• Katarína Smolková,
• Petr Ježek and
• Daniel Horák

Beilstein J. Nanotechnol. 2015, 6, 2290–2299, doi:10.3762/bjnano.6.235

• . Characterization of the nanoparticles The nanoparticles were visualized and analyzed on a Tecnai G2 Spirit Twin transmission electron microscope (TEM; FEI; Brno, Czech Republic) equipped with an energy dispersive spectrometer (EDX; Mahwah, NJ, USA). Bright field TEM imaging (BF), electron diffraction (ED) and

Possibilities and limitations of advanced transmission electron microscopy for carbon-based nanomaterials

• Xiaoxing Ke,
• Carla Bittencourt and
• Gustaaf Van Tendeloo

Beilstein J. Nanotechnol. 2015, 6, 1541–1557, doi:10.3762/bjnano.6.158

• the specimen. When the correction is used in bright-field imaging using a parallel beam the corrector is referred to as “image corrector”. When the Cs corrector is applied to the electron beam before interacting with the specimen, and forms a highly converged electron probe, it is referred to as

Formation of pure Cu nanocrystals upon post-growth annealing of Cu–C material obtained from focused electron beam induced deposition: comparison of different methods

• Aleksandra Szkudlarek,
• Alfredo Rodrigues Vaz,
• Yucheng Zhang,
• Andrzej Rudkowski,
• Czesław Kapusta,
• Rolf Erni,
• Stanislav Moshkalev and
• Ivo Utke

Beilstein J. Nanotechnol. 2015, 6, 1508–1517, doi:10.3762/bjnano.6.156

• precipitate. The inset shows the SAD pattern of Cu fcc nanocrystals. TEM in situ annealing of FEBID rods grown from (hfac)Cu(VTMS). a) Dark field image of an as-deposited freestanding rod. Inset: rod apex with small Cu nanocrystals in carbonaceous matrix. b) Bright-field image of same rod after 270 °C

• annealing and continuous TEM observation (200 keV). Large Cu nanocrystals form inside the rod. c) Bright field image of another rod not observed during the same annealing process. Cu nanocrystals form at the outside surface of the rod. Calculated resistivity from the resistance measurement of a Cu–C line

Influence of the shape and surface oxidation in the magnetization reversal of thin iron nanowires grown by focused electron beam induced deposition

• Luis A. Rodríguez,
• Lorenz Deen,
• Rosa Córdoba,
• César Magén,
• Etienne Snoeck,
• Bert Koopmans and
• José M. De Teresa

Beilstein J. Nanotechnol. 2015, 6, 1319–1331, doi:10.3762/bjnano.6.136

• microstructure were determined by bright field (BF) TEM and high resolution TEM (HRTEM) imaging, and chemical composition of the sections was determined by combining high angle annular dark field (HAADF) imaging and electron energy loss spectroscopy (EELS) in scanning transmission electron microscopy (STEM) mode

Structural transitions in electron beam deposited Co–carbonyl suspended nanowires at high electrical current densities

• Gian Carlo Gazzadi and
• Stefano Frabboni

Beilstein J. Nanotechnol. 2015, 6, 1298–1305, doi:10.3762/bjnano.6.134

• measurements is reported in Figure 3. The bright-field image of SNW 1 is completely changed from the grainy structure observed after deposition. Now it has the typical appearance of a polycrystalline material, with regions of well-defined contrast extending for tens of nanometers along the wire, and separated

• structural transformation that was indeed confirmed by SEM analysis, in Figure 4c. The left-hand half of the wire looks brighter while the right-hand one has become transparent. To deeper investigate the nature of this transition we turned to TEM analysis. As shown by the bright-field image in Figure 5, the

• )–voltage(V) measurements on SNW 1. In the inset, the first I–V measurement taken on the wire as shown in Figure 1a is magnified; (b) SEM image (at 52° tilt angle) of SNW 1 after I–V 1. Bright-field TEM image of SNW 1 after electrical measurements. In the inset, the SAED pattern taken on the circled area

Addition of Zn during the phosphine-based synthesis of indium phospide quantum dots: doping and surface passivation

• Natalia E. Mordvinova,
• Alexander A. Vinokurov,
• Oleg I. Lebedev,
• Tatiana A. Kuznetsova and
• Sergey G. Dorofeev

Beilstein J. Nanotechnol. 2015, 6, 1237–1246, doi:10.3762/bjnano.6.127

• scattering region. This discrepancy can be explained by fact that the contrast in bright-field low-magnification TEM is a mass-thickness contrast, which arises from Rutherford elastic scattering of electrons, rather than a diffraction or an amplitude contrast in the case of dislocations and high resolution

• . In the low-magnification HAADF-STEM image (Figure 4a), the size of the QDs is close to that of the bright-field TEM images (Figure 3). However, upon close inspection using high resolution HAADF-STEM (Figure 4b–g), the core–shell structure of Zn/InP QDs can be clearly distinguished and confirmed. The

• InP QDs. Experimental X-ray powder diffractogram for synthesized InP QDs with different amounts of Zn dopant. (a) Bright-field low-magnification TEM image of non-doped InP QDs and its number-weighted size distribution (upper insert). Ring electron diffraction pattern (lower insert) confirming zinc

Structure and mechanism of the formation of core–shell nanoparticles obtained through a one-step gas-phase synthesis by electron beam evaporation

• Andrey V. Nomoev,
• Sergey P. Bardakhanov,
• Makoto Schreiber,
• Dashima G. Bazarova,
• Nikolai A. Romanov,
• Boris B. Baldanov,
• Bair R. Radnaev and
• Viacheslav V. Syzrantsev

Beilstein J. Nanotechnol. 2015, 6, 874–880, doi:10.3762/bjnano.6.89

• ) detail of the core and shell structure. TEM micrograph of a Cu@silica nanoparticle. a) Bright field, b) dark field. TEM micrograph of a Cu particle with an incomplete shell demonstrating moiré patterns where Cu2O has grown on the Cu. a) View of whole particle, b) detail of the moiré patterns. TEM

Mandibular gnathobases of marine planktonic copepods – feeding tools with complex micro- and nanoscale composite architectures

• Jan Michels and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2015, 6, 674–685, doi:10.3762/bjnano.6.68

• material composition than the rest of the gnathobases. This different appearance can easily be shown in an ordinary way by bright-field microscopy, and it becomes clearly evident when the gnathobases are visualized with scanning electron microscopy (e.g., Figures 2c–e, 3a, 3c–e, 5a, 6a). Already several

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• microscopic analysis of the cells. Three different field-of-views, with a surface area of 74 mm2 each, were randomly selected and the number of all visible cells was counted. Afterwards, fluorescence (FITC) or bright-field microscopy (Prussian blue) was completed to measure the presence of cells loaded with

The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

• Vladimir V. Breus,
• Anna Pietuch,
• Marco Tarantola,
• Thomas Basché and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26

• enhanced fluorescent signal due to moving species in the cell, while the green channel corresponds to the amplified cell autofluorescence (Supporting Information File 1, Figure S4). Additionally, the corresponding transmission bright-field micrographs are shown below each fluorescence image (Figure 3). For

• cadmium acetate (c). Composite images of QD fluorescence (red) and cell autofluorescence (green) together with corresponding transmission bright-field micrographs. The fluorescence signals from QDs and cells are extracted from the overall fluorescent signal by applying standard deviation (for QDs) and

Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• the same tissue is possible in direct context of the autoradiographic NP signals [56]. Fluorescence microscopy Conventional fluorescence microscopy is an essential tool in countless biomedical research applications and possesses a resolution similar to that of bright-field light microscopy [35][73

• which is insufficient for a complete histological assessment by a pathologist. Another approach is to compare and merge images of fluorescence microscopy and bright field microscopy by illuminating the same slide and location used for fluorescence microscopy with transmitted light [20][77]. As a further

Multifunctional layered magnetic composites

• Maria Siglreitmeier,
• Baohu Wu,
• Tina Kollmann,
• Martin Neubauer,
• Gergely Nagy,
• Dietmar Schwahn,
• Vitaliy Pipich,
• Damien Faivre,
• Dirk Zahn,
• Andreas Fery and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2015, 6, 134–148, doi:10.3762/bjnano.6.13

• )-loaded matrix was washed with water and placed in 0.1 M NaOH solution for 150 min. Sample characterizations Samples of Coomassie-stained thin cuts were observed under bright field transmission mode by using a Zeiss optical microscope equipped with a video camera (AxioCam MRc5). Fluorescent labeled

Inorganic Janus particles for biomedical applications

• Isabel Schick,
• Steffen Lorenz,
• Dominik Gehrig,
• Stefan Tenzer,
• Wiebke Storck,
• Karl Fischer,
• Dennis Strand,
• Frédéric Laquai and
• Wolfgang Tremel

Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244

• of Chemistry. a) UV–vis spectra of Au@Fe3O4 nanoparticles corresponding to schematic representations in b). The scheme illustrates the shape evolution of the particles during heating (Au: dark gray, Fe3O4: bright gray). Reproduced with permission from [72]. Copyright 2008 WILEY-VCH. TEM bright field

• images of Au nanoparticles with different diameters (a) 4 nm, (b) 8 nm, and (c) 15 nm; (d) 2D superlattice of 8 nm Au nanoparticles. TEM bright field images of Au@MnO and Au@Fe3O4 heterodimer-nanoparticles: (a) 9@18 nm Au@MnO, (b) 4@22 nm Au@MnO, (c) 9@15 nm Au@Fe3O4, and (d) 7@20 nm Au@Fe3O4. Domain

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• cultured in the presence of RPMI/FCS (A) or in the presence of adipogenic-differentiation medium (B). Influence of Ag-NP/ Ag+ ions on the adipogenic differentiation of hMSCs. After 14 d of cell culture (bright-field and fluorescence images), Bodipy493/503 staining was used to visualize lipid vacuoles in

• (Adiponectin 191 ± 13 ng·mL−1; cells cultured without silver, dashed line). The asterisks (*) indicate significant differences with respect to the control (*p < 0.05, **p < 0.01, ***p < 0.001). Influence of Ag-NP/ Ag+ ions on hMSCs during osteogenic differentiation. After 21 d of cell culture (bright-field

• cultured without silver, dashed line). The asterisks (*) indicate significant differences with respect to the control (*p < 0.05, ***p < 0.001). Influence of Ag-NP/Ag+ ions on the chondrogenic differentiation of hMSCs. After 21 d of cell culture under chondrogenic conditions (bright-field images), alcian