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Search for "cell culture" in Full Text gives 163 result(s) in Beilstein Journal of Nanotechnology.
Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy
Beilstein J. Nanotechnol. 2021, 12, 1372–1379, doi:10.3762/bjnano.12.101
- Shijiazhuang Hongwei Biotechnology Co., Ltd. AF488-WGA and Hoechst 33342 were purchased from Thermo Fisher Scientific. All medicals and reagents were used without further treatment. Cell culture HPDE6-C7, AsPc-1 and BxPC-3 cells were cultured in RPMI-1640 with 10% fetal bovine serum and 1% double antibody. MIA
Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99
- potential values is the interaction between NPs and serum proteins present in DMEM [30]. In cell culture media, NPs agglomerate with serum proteins and are therefore recruited in cells via the protein corona effect, which increases the bioavailability of NPs by many folds [31]. PMA-coated samples have a
- calculated by the formula given in Equation 4: Cell culture The cell lines human malignant melanoma (HT144, ATCC® HTB-63™) and human hepatocellular carcinoma (HepG2, ATCC®HB-8065™) were used in this study. The cells were grown in DMEM supplemented with 10% FCS and 1% GPPS in a humidified incubator (37 °C
Identifying diverse metal oxide nanomaterials with lethal effects on embryonic zebrafish using machine learning
Beilstein J. Nanotechnol. 2021, 12, 1297–1325, doi:10.3762/bjnano.12.97
- […] with the convenience of cell culture” [25] and that “zebrafish exhibit remarkable similarity to other high-order vertebrates including humans” [23]. Hence, the Nanomaterial Biological Interactions (NBI) Knowledgebase [26], a publicly available database of ENM embryonic zebrafish test results for
Self-assembly of amino acids toward functional biomaterials
Beilstein J. Nanotechnol. 2021, 12, 1140–1150, doi:10.3762/bjnano.12.85
- physical and chemical properties, and great potential in cell culture, photocatalysis, drug delivery, and antibacterial applications [27]. The Fmoc modification of a single amino acid is the simplest building block, among which Fmoc-phenylalanine is the most studied due to its good hydrocoagulant
Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension
Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62
- , strongly slows down the dissolution [35]. In another study, Zook et al. investigated the dissolution in DMEM cell culture medium and reported the dissolution of different polymer-coated Ag-NPs. It was found that the dissolution was increased in cell culture media in comparison to inorganic salt solutions
- signaling pathway components. Experimental All cell culture plates as well as cell culture media were purchased form SPL Company Ltd. and Gibco Company (UK), respectively. Sources of other materials are mentioned in the text. The Ag-NPs used in this study were produced by an American company (US Research
- Sengstock and co-workers [11]. Sengstock et al. reported that Ag-NPs with a size of 80 nm do not enter the cell nucleus and that Ag agglomerates appeared primarily within the endo-lysosomes. In the other words, the agglomerated Ag-NPs were visible in a region close to the cell nucleus but not in the cell
Recent progress in magnetic applications for micro- and nanorobots
Beilstein J. Nanotechnol. 2021, 12, 744–755, doi:10.3762/bjnano.12.58
- in cell culture medium), if the trajectory of the target motion is preset, it could follow the path and transfer the payload with the help of the rotating magnetic field. Different from the device in [42], an electromagnetic control system composed of three pairs of Helmholtz coils was used to
Fate and transformation of silver nanoparticles in different biological conditions
Beilstein J. Nanotechnol. 2021, 12, 665–679, doi:10.3762/bjnano.12.53
- transformation, dissolution, and degradation behaviour of different types of AgNPs were evaluated in systems modelled to represent biological environments relevant for oral administration, as well as in cell culture media and tissue compartments obtained from animal models. A multimethod approach was employed by
A review on nanostructured silver as a basic ingredient in medicine: physicochemical parameters and characterization
Beilstein J. Nanotechnol. 2021, 12, 440–461, doi:10.3762/bjnano.12.36
- condition characterized by pigmentary changes secondary to exposure to silver salts which accumulate in the skin and mucous membranes. The toxicity of AgNPs is closely related to the release of Ag+ [57]. Studies with human mesenchymal stem cells (hMSCs) treated, under cell culture conditions, with different
The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors
Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31
- chitosan with the carboxylic groups of poly(glutamic acid). EDC/NHS chemistry was used for conjugating the mAbs on the surface of the nanoscale carriers. The cross-linked nanoparticles showed superior antiproliferative activity compared to NPs without ligands. In vitro cell culture studies on A549 cells
Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells
Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23
- with 10% fetal bovine serum and counted using a hemocytometer. This method led to more than 97% pure and live cells. Freshly isolated PBMCs were adjusted to 2.5 × 105 in 24 well-plates (Costar, ME, USA) for cell culture and bio-uptake experiments, and 2.5 × 104 cells in 96 well-plates (Corning
- culture plates at a density of 2.5 × 105 cells. PVP-AgNP and AgNO3 stock (25 mg·L−1) were diluted with cell-culture water (Sigma-Aldrich, Taufkirchen, Germany) and added to attain final concentrations of 10, 100, 500, and 1000 µg·L−1 prior to exposure. The AgNP solution was sonicated for 20 min to reduce
- Incorporated, NY, USA) for cell viability and metabolic activity test. Uptake of Ag into PBMCs and mass balance ICP-MS was used to quantify the uptake of Ag in PBMCs after exposure to PVP-AgNPs and AgNO3 (as control). Freshly isolated PBMCs were resuspended in RPMI medium and then seeded in 24-well cell
The nanomorphology of cell surfaces of adhered osteoblasts
Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20
- coatings of positively charged plasma-polymerized allylamine (PPAAm) or negatively charged Au were used, see below. Viability tests (MTS) were performed on glass, sputter-deposited Au, polydimethylsiloxane (PDMS), and PPAAm coating with native titanium and cell culture plastic as reference. Cell viability
- adhering directly across the border between Au and glass, the cells apparently exhibited no preference. Cell culture For the SICM experiments, human osteoblast-like cells of the cell line MG-63 (American Type Culture Collection ATCC®, CRL1427™, Bethesda, USA) were used. This cell line has been successfully
Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy
Beilstein J. Nanotechnol. 2021, 12, 172–179, doi:10.3762/bjnano.12.13
- monolayers were infected with SARS-CoV-2 at a multiplicity of infection (MOI) of approximately 1 or mock-infected using cell culture medium. Following an incubation period of 18 h in a cell culture incubator (37 °C), cells were washed with 0.1 M sodium cacodylate (NaCac, pH 7.4) and fixed in 2% (v/v
A review on the green and sustainable synthesis of silver nanoparticles and one-dimensional silver nanostructures
Beilstein J. Nanotechnol. 2021, 12, 102–136, doi:10.3762/bjnano.12.9
- synthesis of AgNPs, as they can accumulate Ag atoms on the cell walls [104]. The bacteriogenic synthesis process can be either extracellular or intracellular. AgNPs are synthesized either by the biomass [267] or the cell culture supernatant [259]. AgNPs synthesized using bacteriogenic pathogens are commonly
- amides corresponding to cellular proteins and enzymes, are responsible for the synthesis and stabilization of AgNPs (Table 2). These components are present in both the biomass and the cell culture supernatant. It was previously demonstrated that the synthesis rate can be faster for some bacterial strains
- including Escherichia coli (E. coli) and K. Pneumonia when the cell culture supernatant is used [268]. However, the main downside of bacteriogenic synthesis is the slow synthesis rate and large size distribution compared to other green methods [104][259]. The applications of bacteria-synthesized AgNPs can
Effect of different silica coatings on the toxicity of upconversion nanoparticles on RAW 264.7 macrophage cells
Beilstein J. Nanotechnol. 2021, 12, 35–48, doi:10.3762/bjnano.12.3
- inductively coupled plasma optical emission spectrometry (ICP-OES). Before the cell experiments, the stability of the particles in cell culture media was investigated via DLS and ELS. The cytotoxicity of the UCNPs was determined by MTT assays and cell cycle analysis. The UCNP uptake potential was evaluated by
- -functionalized particles is small enough for the particles to be internalized via the endocytic uptake [51]. (3-Aminopropyl)trimethoxysilane (APS) was not chosen as the amine ligand due to the increased aggregation of APS-functionalized particles in the cell culture medium [51]. In addition, particles with a
- ionic strength of the cell culture medium (I = 168 mmol/L) reduces the electrostatic stabilization. Besides, the proteins in the DMEM cell culture medium supplemented with 10% FBS contribute to the measured hydrodynamic diameter values [51]. FBS consists mostly of bovine serum albumin. The Z-average
PEG/PEI-functionalized single-walled carbon nanotubes as delivery carriers for doxorubicin: synthesis, characterization, and in vitro evaluation
Beilstein J. Nanotechnol. 2020, 11, 1728–1741, doi:10.3762/bjnano.11.155
- . Polyethyleneimine (PEI, Mw = 600 Da) was purchased from Aladdin Chemistry Co., Ltd. Doxorubicin hydrochloride (DOX) was purchased from Dalian Meilun Biological Technology Co., Ltd (Dalian, China). Cell culture medium RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin solution and fluorescent Hoechst 33342
- grade. Cell culture The human breast cancer cell line MCF-7 used in this study was obtained from American Type Culture Collection (ATCC, Manassas, VA), and cultured in RPMI 1640 medium containing 10% (v/v) fetal bovine serum (FBS) and 1% penicillin–streptomycin at 37 °C in a humidified atmosphere with 5
Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system
Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150
- of 75 kV. The particle size distribution of the HAp nanoparticles was measured via NTA (NanoSight NS10, Malvern). Cell culture HL-1 is a cell line derived from mouse atrial myocytes, which was originally isolated and characterized by Dr. Claycomb (University of Louisiana) [39]. HL-1 cells (murine
- cardiomyocytes) were seeded and grown in Claycomb culture medium (Sigma) supplemented with 10% of fetal bovine serum (Sigma), 0.1 mM of norepinephrine (Sigma), 2 mM of ʟ-glutamine (Wako), and 1% of penicillin/streptomycin (Gibco) as previously published [39]. All cell culture dishes and plates were coated with a
Antimicrobial metal-based nanoparticles: a review on their synthesis, types and antimicrobial action
Beilstein J. Nanotechnol. 2020, 11, 1450–1469, doi:10.3762/bjnano.11.129
- , eliminating an additional step to prevent particle aggregation [86]. In addition, cell culture procedures are not necessary in this case, which allows for the large-scale synthesis of nanoparticles in a non-aseptic environment [87]. Furthermore, plant-based processes are cost-effective and safe for humans and
Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements
Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94
- increase in macrophages. The general consideration is that polymer coating offers colloidal stability, but in fact PVA-coated SPIONs are only stable in water at a certain pH value. In cell culture medium they agglomerate. Studies showed that the components of the medium, and not the calf serum added to the
- medium, led to nanoparticle agglomeration. Dulbecco's Modified Eagle Medium (DMEM) with added serum yielded the highest nanoparticle stability compared to other media [54][87]. Also, PVA-coated SPIONs were not internalized by cells when the cell culture medium had serum in it. Without serum cell uptake
- stability in cell culture media, it was demonstrated that poly(methacrylic acid)-coated SPIONs and citric acid-coated SPIONs are stable in common cell culture media (DMEM, RPMI), when serum was added, but produced aggregates larger than 1 µm in simple media and in phosphate buffer [54]. Stability and
Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy
Beilstein J. Nanotechnol. 2020, 11, 568–582, doi:10.3762/bjnano.11.45
- during very early diagnosis of cancer at the level of single living cells. Experimental Cell culture Three ovarian cell lines, HOSEpiC (human ovarian epithelial cell line, BeNa Culture Collection, Beijing, China), OVCAR-3 (human cancerous ovarian cell line, BeNa Culture Collection, Beijing, China) and HO
- cells was analyzed by cell culture insert (Corning, 8.0 μm pore size) coated with a PET membrane according to the instructions of the manufacturer. A total of 1 × 104 cells suspended in 500 µL serum-free medium was loaded into the upper chambers, and the bottom chamber was filled with 500 μL medium
Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine
Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25
- uptake. For such studies, the nanoparticle dispersion, the cell culture conditions, the cell line investigated, and the methods used to characterize the uptake mechanisms are all crucial. Unfortunately, there are often no agreements on how to perform uptake studies in a standardized way. Recently, this
Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications
Beilstein J. Nanotechnol. 2020, 11, 213–224, doi:10.3762/bjnano.11.16
- triglyceride) was provided by Hansen & Rosenthal KG (Hamburg, Germany). Kolliphor HS 15 (macrogol 15 hydroxystearate) was provided by BASF SE (Ludwigshafen, Germany). Sodium chloride was purchased from Grüssing GmbH (Filsum, Germany), the components for the cell culture medium Dulbecco’s Modified Eagle Medium
- guidelines Q1A. Toxicity on NHDF and 3T3 cells Approximately 20,000 NHDF cells and 10,000 3T3 cells were seeded in 96 well plates and grown for 24 h at 37 °C and 5% CO2 in 100 µL of the corresponding cell culture media shown in Table 3. After adding 50 µL aseptic and 0.2 µm of the sterile, filtered and
- . The values in brackets refer to the concentration of MCT + Kolliphor HS15 in NE25, NE50 and NE100. Composition of the cell culture media. Supporting Information Supporting Information File 2: Additional figures.
Internalization mechanisms of cell-penetrating peptides
Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10
- contrast to the common cationic CPPs, Arukuusk et al. [88] suggest negatively charged CPPs as oligonucleotide carriers. The CPPs are not inherently anionic, but confer a negative charge after they are complexed with a nucleic acid in cell culture media. The group has already demonstrated that the uptake of
The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency
Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250
- images were analysed using ImageJ software (v1.49p, http://rsb.info.nih.gov/ij). Cell studies HCT-116 and RAW 264.7 cell lines were cultured in complete media (McCoy’s 5A or high glucose DMEM, respectively) under standard conditions for cell culture (5% v/v CO2 in air, 37 °C). Further details of the
- materials used is provided in Supporting Information File 1, Section SI1.1.2. Preparation of double-concentrated cell culture growth media. 5.95 g McCoy’s 5A powder or 6.75 g of DMEM powder, respectively, were dissolved in 175 mL of distilled water followed by addition of 3 g of HEPES. The pH value was then
Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery
Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249
- Institutional Committee of Science and Research Ethics. The number of the ethical permission is: 17458/2012/EKU. After extraction, the teeth were immediately placed into a sterile cell culture medium. The viable periodontal fibers were removed from the tooth surface, put into a sterile box with a sterile blade
- serum (FBS, Gibco, USA), 2 mM ʟ-glutamine (Gibco, USA), 100 units/mL penicillin and 100 mg/mL streptomycin (Gibco, USA). When the cell culture became subconfluent, it was passaged at a ratio of 1:20 using a 0.05% trypsin/EDTA solution. Cell viability assay Before performing the cell viability assay, the
Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells
Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246
- therapeutic outcomes. Experimental Materials Fmoc-protected amino acids, piperazine, HCTU, HOBt, DMF were all from AGTC (Hessle, UK). Rink Amide MBHA resin was from Novabiochem (UK). HPLC solvents and all cell culture media were from Sigma-Aldrich (Poole, UK). Lipids were from Avanti Polar Lipids (USA
- . Samples were incubated at 4, 25 or 37 °C. After 0, 24 and 72 hours the samples were transferred to cuvettes and measured for size and PDI as described above. Cell culture and transfection The adherent, non-small cell cancer cell line A549 was stably transfected with a plasmid encoding HA epitope-tagged
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