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Search for "enzymology" in Full Text gives 15 result(s) in Beilstein Journal of Organic Chemistry.
Activity assays of NnlA homologs suggest the natural product N-nitroglycine is degraded by diverse bacteria
Beilstein J. Org. Chem. 2024, 20, 830–840, doi:10.3762/bjoc.20.75
- protect bacteria from exposure to NNG. Keywords: enzymology; natural products; nitramine; N–N bond; Introduction Degradation of nitramines (R–N(R′)NO2; R′ = H or alkyl) has been well studied in the context of the environmental degradation of explosive cyclic nitramines [1][2]. The cyclic nitramines
Chemoenzymatic synthesis of macrocyclic peptides and polyketides via thioesterase-catalyzed macrocyclization
Beilstein J. Org. Chem. 2024, 20, 721–733, doi:10.3762/bjoc.20.66
- aforementioned NPRS macrocycles, the synthesis of macrocylic polyketides and PKS/NRPS hybrids is more challenging due to the absence of a streamlined preparation strategy such as solid-phase peptide synthesis (SPPS). In enzymology studies, it was common to hydrolyze the cyclic natural products in order to obtain
Development of a chemical scaffold for inhibiting nonribosomal peptide synthetases in live bacterial cells
Beilstein J. Org. Chem. 2024, 20, 445–451, doi:10.3762/bjoc.20.39
- Japan Foundation for Applied Enzymology and the Research Foundation for Pharmaceutical Sciences to F.I. and a Grant-in Aid for Scientific Research on Innovative Areas (17H06401 to H.K.) and for Transformative Research Area (A) (23H04882 to H.K.) from the Ministry of Education Culture, Sports, Science
Recommendations for performing measurements of apparent equilibrium constants of enzyme-catalyzed reactions and for reporting the results of these measurements
Beilstein J. Org. Chem. 2023, 19, 303–316, doi:10.3762/bjoc.19.26
- general discussion of various aspects of these equilibrium measurements as well as STRENDA (Standards for Reporting Enzymology Data) recommendations regarding the measurements and the reporting of results. Keywords: enzyme-catalyzed reactions; equilibrium constant; standards; thermodynamics; Perspective
- 1. Introduction and motivation for recommendations The aim of the STRENDA (Standards for Reporting Enzymology Data) Commission [1] is to establish standards for reporting the results of measurements related to enzymology with the aim to improve the quality and the findability, accessibility
Enzymes in biosynthesis
Beilstein J. Org. Chem. 2022, 18, 1131–1132, doi:10.3762/bjoc.18.116
- , requiring a sophisticated enzymology [4]. Recent developments show us that there is still much more to discover, e.g., altemicidin was shown to be enzymatically constructed from NAD+ and SAM that usually serve as enzyme cosubstrates in redox transformations and methylations but are rarely used to construct
Synthesis, docking study and biological evaluation of ᴅ-fructofuranosyl and ᴅ-tagatofuranosyl sulfones as potential inhibitors of the mycobacterial galactan synthesis targeting the galactofuranosyltransferase GlfT2
Beilstein J. Org. Chem. 2020, 16, 1853–1862, doi:10.3762/bjoc.16.152
- –10 mM. Interestingly, while compound 1bα showed a dose-dependent inhibitory effect in this experiment, compound 3a did not (Table 2). Although the crude system that we used in the enzymology experiments precludes precise kinetic characterization of the studied inhibitors on the target enzyme, GlfT2
Opening up connectivity between documents, structures and bioactivity
Beilstein J. Org. Chem. 2020, 16, 596–606, doi:10.3762/bjoc.16.54
- . However, in the broader context of bioactive chemistry, it becomes indivisible from the related domains of chemical biology (directed towards mechanistic insight rather that direct drug discovery), enzymology, pharmacology, and toxicology in addition to the development of insecticides or herbicides
Molecular basis for the plasticity of aromatic prenyltransferases in hapalindole biosynthesis
Beilstein J. Org. Chem. 2019, 15, 1545–1551, doi:10.3762/bjoc.15.157
- within the PTases, as the natural metalloprotein has been utilized as a model to create an artificial metalloprotein [36][37]. The increasing knowledge obtained from the X-ray structural studies of the PTases will contribute to the development of the enzymology and the chemoenzymatic syntheses of
Back to the future: Why we need enzymology to build a synthetic metabolism of the future
Beilstein J. Org. Chem. 2019, 15, 551–557, doi:10.3762/bjoc.15.49
- to build synthetic metabolism. Here I discuss the current challenges and limitations in synthetic metabolic engineering and elucidate how modern day enzymology can help to build a synthetic metabolism of the future. Keywords: enzymes; in vitro biochemistry; metabolic engineering; synthetic biology
- bottom-up might represent a valuable alternative strategy [6]. Linking enzymology and synthetic biology In summary, synthetic biology can develop its full potential, if it becomes able to harness the diversity of the millions of different enzyme variants and homologs that naturally exist. While such
- information is collected and made available by many enzymology and biochemistry laboratories worldwide in a community effort, it is not provided in an optimal way so that it can be used for the synthetic metabolism community. How could this apparent gap be bridged? First, it will be necessary to collect
New standards for collecting and fitting steady state kinetic data
Beilstein J. Org. Chem. 2019, 15, 16–29, doi:10.3762/bjoc.15.2
- quantitative analysis, fulfilling the major goal of their work [1][2]. Estimating the binding affinity for the substrate as KS was an added bonus. These were profound discoveries that laid the foundation for enzymology throughout the 20th century. The Michaelis–Menten equation was originally derived assuming
- parameters takes on new significance in the current era of enzymology where the emphasis is on relating the parameters to individual rate constants and to structural and chemical transitions for each reaction in the pathway. While kcat/Km can be directly interpreted in terms of enzyme specificity, it also
Pd-Catalyzed microwave-assisted synthesis of phosphonated 13α-estrones as potential OATP2B1, 17β-HSD1 and/or STS inhibitors
Beilstein J. Org. Chem. 2018, 14, 2838–2845, doi:10.3762/bjoc.14.262
- and Economics, H-1521 Budapest, Hungary 1st Department of Medicine, University of Szeged, Korányi fasor 8–10, H-6720 Szeged, Hungary Membrane protein research group, Institute of Enzymology, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Magyar tudósok körútja 2, H-1117 Budapest
Synthesis and in vitro biochemical evaluation of oxime bond-linked daunorubicin–GnRH-III conjugates developed for targeted drug delivery
Beilstein J. Org. Chem. 2018, 14, 756–771, doi:10.3762/bjoc.14.64
- , Hungary Research Centre for Natural Sciences, Institute of Enzymology, Hungarian Academy of Sciences, 1117 Budapest, Hungary MTA-ELTE Protein Modelling Research Group, Hungarian Academy of Sciences, Eötvös L. University, 1117 Budapest, Hungary Department of Biopharmacy, Faculty of Pharmacy, University of
Sulfation and amidinohydrolysis in the biosynthesis of giant linear polyenes
Beilstein J. Org. Chem. 2017, 13, 2408–2415, doi:10.3762/bjoc.13.238
- in a genome-led approach to the uncovering of novel enzymology in the biosynthetic pathways to antibiotic natural products [23][24][25][26]. Here, we have used in-house whole-genome sequencing to characterise the closely-related gene clusters to the sulfated antifungal linear polyenes clethramycin
Biosynthesis of oxygen and nitrogen-containing heterocycles in polyketides
Beilstein J. Org. Chem. 2016, 12, 1512–1550, doi:10.3762/bjoc.12.148
- all classes and they often contribute significantly to their biological activity. Progress in recent years has led to a much better understanding of their biosynthesis. In this context, plenty of novel enzymology has been discovered, suggesting that these pathways are an attractive target for future
- studies. Keywords: biosynthesis; chemoenzymatic synthesis; enzymology; heterocycles; polyketides; Introduction Heterocycles Heterocycles are important structural elements, which are present in natural products from all classes and also in many biologically active synthetic compounds. They often
- cyclisation modes triggers the interest on the responsible enzymes. Due to the relevance of heterocycles, understanding the enzymology of heterocycle formation is also an important milestone on the way to using the enzymes as chemoenzymatic tools in natural product synthesis and medicinal chemistry [6][7
OligArch: A software tool to allow artificially expanded genetic information systems (AEGIS) to guide the autonomous self-assembly of long DNA constructs from multiple DNA single strands
Beilstein J. Org. Chem. 2014, 10, 1826–1833, doi:10.3762/bjoc.10.192
- pairs, respectively. The availability of this new concept for the assembly of large DNA constructs from multiple inexpensive single-stranded oligonucleotides, together with the chemistry and enzymology needed to convert unnatural assemblies into entirely natural assemblies, creates the need for a
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