Temperature-tunable lasing from dye-doped chiral microdroplets encapsulated in a thin polymeric film

• Gia Petriashvili,
• Mauro Daniel Luigi Bruno,
• Maria Penelope De Santo and
• Riccardo Barberi

Beilstein J. Nanotechnol. 2018, 9, 379–383, doi:10.3762/bjnano.9.37

• goes in solution and the pitch of the cholesteric helix shortens. For lasing experiments, the dye fluorescence spectrum must overlap the whole shifting interval of the PBG. The selected fluorescent dye is 2-[2-[4-(dimethylamino)phenyl]ethenyl]-6-methyl-4H-pyran-4-ylidene]propanedinitrile (DCM) from

• Exciton. It is added to the chiral mixture with a concentration of 0.3 wt %. Figure 2 shows the fluorescence spectrum of the DCM dye. First, the dye-doped CLC mixture is used to prepare the emulsion. With respect to [17], the emulsion is prepared with the same procedure, but it contains only one type of

• the temperature and on the spectral position of the dye fluorescence with respect to that of PBG, laser emission can be observed at the right and/or left edge of the PBG. Then, it is impossible to provide an estimate of the droplets temperature using the spectral position of the laser wavelength

Photocatalytic and adsorption properties of TiO₂-pillared montmorillonite obtained by hydrothermally activated intercalation of titanium polyhydroxo complexes

• Mikhail F. Butman,
• Nikolay L. Ovchinnikov,
• Nikita S. Karasev,
• Nataliya E. Kochkina,
• Alexander V. Agafonov and
• Alexandr V. Vinogradov

Beilstein J. Nanotechnol. 2018, 9, 364–378, doi:10.3762/bjnano.9.36

• intercalation the solutions were diluted by slowly adding deionized water to yield solutions with a residual Ti4+ concentration of 0.56 M. Prior to use the intercalation solutions were aged for 3 hours at 20 °C, resulting in the formation of titanium polyhydroxo complexes. Figure 14 shows the fluorescence

• TiO2-pillared MM with the commercial photocatalyst Degussa P25 upon degradation of methyl orange (MO) and rhodamine B (RhB) dyes in aqueous solution. SEM image of the surface of original MM. The fluorescence spectrum of an intercalating solution of titanium polyhydroxo complexes. The dynamic light

Sugarcane juice derived carbon dot–graphitic carbon nitride composites for bisphenol A degradation under sunlight irradiation

• Lan Ching Sim,
• Jing Lin Wong,
• Chen Hong Hak,
• Jun Yan Tai,
• Kah Hon Leong and
• Pichiah Saravanan

Beilstein J. Nanotechnol. 2018, 9, 353–363, doi:10.3762/bjnano.9.35

• -green fluorescence under excitation at 365 nm with a UV lamp. The CD solution was tested with different excitation wavelengths to evaluate the ideal excitation spectrum that initiates the photoluminescence (PL) properties. Figure 3b displays wide, broad PL emission peak across the UV to visible spectrum

• consistent with previous reports [61]. A recent study by Wen and co-workers suggested that most of the CDs and graphene quantum dots (GQDs) do not have detectable upconversion fluorescence. The frequently cited UCPL properties could originate from the normal fluorescence excited by the leaking component from

• the second diffraction in the monochromater of the fluorescence spectrophotometer [62]. As shown in Figure 3c, the pure g-C3N4 showed expected absorption peaks at 265 nm and 340 nm due to the π–π* or n–π* electronic transitions in the conjugated aromatic s-heptazine ring system of g-C3N4 [63]. The

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• more QDs compared with cells incubated in complete medium. At a QD concentration of 2 nM, 100% of the cells were labelled with QDs in serum-free medium, whereas a 100% positive cell population was achieved after incubation with 16 nM QDs in complete medium (Figure 1A). Fluorescence intensity analysis

• h was confirmed by fluorescence intensity analysis (Figure 3B). On the contrary, 100% of MSCs that were labelled in serum-free conditions remained QD positive until 72 h of incubation (Figure 3C). Despite the fact that 100% of the MSC population was QD positive in serum-free medium until 72 h, we

• observed a 5.5-fold/5-fold decrease in the fluorescence intensity, respectively, indicating that QD elimination occurs (Figure 3D). As mentioned previously, a significantly increased intracellular accumulation of QDs was observed in serum-free medium (Figure 1A,B), and the QD elimination effect was

Wafer-scale bioactive substrate patterning by chemical lift-off lithography

• Chong-You Chen,
• Chang-Ming Wang,
• Hsiang-Hua Li,
• Hong-Hseng Chan and
• Wei-Ssu Liao

Beilstein J. Nanotechnol. 2018, 9, 311–320, doi:10.3762/bjnano.9.31

• thiolate probe solutions were quickly dropped onto the surfaces to anchor the biological probes into the post-chemical lift-off regions. After typically 1 h of incubation, the substrates were gently rinsed by deionized water, immersed in buffer solution, and stored at 4 °C in the dark. Fluorescence image

• recording. An epifluorescence microscope (Axio Imager, M2, Carl Zeiss Microscopy, Jena, Germany) equipped with an X-Cite® 120 LED (Lumen Dynamics Group Inc., Mississauga, Canada) lamp and a fluorescence filter set with excitation and emission wavelengths of 480 ± 15 nm and 535 ± 20 nm, respectively, was

• used. The relative fluorescence intensity was processed with the rectangle function in ZEN 2012 (blue edition) Service Pack 2 software (Carl Zeiss Microscopy, Jena, Germany). Atomic force microscopy characterization. The bioactive substrate fabrication process was step-wisely characterized by the

Co-reductive fabrication of carbon nanodots with high quantum yield for bioimaging of bacteria

• Jiajun Wang,
• Xia Liu,
• Gesmi Milcovich,
• Tzu-Yu Chen,
• Edel Durack,
• Sarah Mallen,
• Yongming Ruan,
• Xuexiang Weng and
• Sarah P. Hudson

Beilstein J. Nanotechnol. 2018, 9, 137–145, doi:10.3762/bjnano.9.16

• 2% to 24% following reduction with sodium borohydride (NaBH4). The same results were confirmed by Shen and Tian's group [15][16]. It was also reported that the fluorescence intensity of graphene quantum dots reduced by hydrazine hydrate (N2H4) can be enhanced to more than two times that of the

• sp2-bonded carbon atoms [36], which agrees well with the proposed co-reduction pathway to effectively produce more reduced carbon with a higher C-dot QY. UV–vis absorption and fluorescence properties were studied at room temperature to explore the optical properties of the three optimized C-dots. As

• time-resolved decay curves of Sa and Sb are well fitted with a tri-exponential function, while that of Se is fitted with a bi-exponential function. The fluorescence characteristic parameters, including the excitation (λex) and emission (λem) wavelengths, quantum yield (Φ), lifetime components (τ1, τ2

Atomic layer deposition and properties of ZrO₂/Fe₂O₃ thin films

• Kristjan Kalam,
• Helina Seemen,
• Peeter Ritslaid,
• Mihkel Rähn,
• Aile Tamm,
• Kaupo Kukli,
• Aarne Kasikov,
• Joosep Link,
• Raivo Stern,
• Salvador Dueñas,
• Helena Castán and
• Héctor García

Beilstein J. Nanotechnol. 2018, 9, 119–128, doi:10.3762/bjnano.9.14

• modelling of samples in the range of 300–1000 nm. An X-ray fluorescence (XRF) spectrometer (Rigaku, ZSX 400) with the software program ZSX (version 5.55) was used to evaluate the elemental composition of the films. The surface morphology of the films and a cross-section of an ALD coated stack were evaluated

• between 15 and 40 nm. Regarding the composition, the cation ratio of mixed films in the as-deposited state, measured by X-ray fluorescence spectrometry, varied from Zr/Fe 0.15–50. Table 1 gives a list of the samples with a description of the growth cycle sequences as well as the observed range of

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• spectrometry, scintigraphic peritoneography and fluorescence microscopy. All experiments were performed contemporaneous with conventional PIPAC for the purpose of comparison. Furthermore, a first proof of concept was simulated in anesthetised German Landrace pigs. Results: HINAT provides a nanometre-sized (63

Patterning of supported gold monolayers via chemical lift-off lithography

• Liane S. Slaughter,
• Kevin M. Cheung,
• Sami Kaappa,
• Huan H. Cao,
• Qing Yang,
• Thomas D. Young,
• Andrew C. Serino,
• Sami Malola,
• Jana M. Olson,
• Stephan Link,
• Hannu Häkkinen,
• Anne M. Andrews and
• Paul S. Weiss

Beilstein J. Nanotechnol. 2017, 8, 2648–2661, doi:10.3762/bjnano.8.265

• supported Au–thiolate layers. The patterning of these layers laterally encodes their functionality, as demonstrated by a fluorescence-based approach that relies on dye-labeled complementary DNA hybridization. Supported thin Au films can be patterned via features on PDMS stamps (controlled contact), using

• the nanometer-scale heights of the supported Au monolayers through scanning probe microscopy, as well as the exploration of spatially encoded functionality using fluorescence microscopy. Otherwise, when topographically patterned PDMS stamps are used to pattern Au monolayers, the traits of the latter

• regions containing Au–alkanethiolates appeared bright in fluorescence microscopy images (Figure 2). Using this straightforward functionalization and visualization method, we investigated patterns of lifted-off Au monolayers on PDMS as substrates for DNA recognition. Upon hybridization of dye-labeled

PTFE-based microreactor system for the continuous synthesis of full-visible-spectrum emitting cesium lead halide perovskite nanocrystals

• Chengxi Zhang,
• Weiling Luan,
• Yuhang Yin and
• Fuqian Yang

Beilstein J. Nanotechnol. 2017, 8, 2521–2529, doi:10.3762/bjnano.8.252

• nanocrystals are continuously synthesized via a microreactor system consisting of poly(tetrafluoroethylene) (PTFE) capillaries. The synthesized nanocrystals possess excellent optical properties, including a full width at half maximum of 19–35 nm, high fluorescence quantum yield of 47.8–90.55%, and

• have constructed a PTFE-based microreactor system in which CsPbX3 nanocrystals are continuously synthesized. The fluorescence characteristics of the CsPbX3 nanocrystals can be tuned via the changes in the synthesis temperature and the halide composition (Br, I). The photoluminescence (PL) emission

• spectra of the CsPbX3 nanocrystals cover the range of 450–700 nm. The prepared nanocrystals possess excellent optical properties with a FWHM of 19–35 nm and a high fluorescence quantum yield (FLQY) of 47.8–90.55%. The CsPbX3 nanocrystals prepared by the PTFE-based microreactor system exhibit three

Interface conditions of roughness-induced superoleophilic and superoleophobic surfaces immersed in hexadecane and ethylene glycol

• Yifan Li,
• Yunlu Pan and
• Xuezeng Zhao

Beilstein J. Nanotechnol. 2017, 8, 2504–2514, doi:10.3762/bjnano.8.250

• Table 1. For the influence of roughness, the Léger group [15] studied slip lengths on octadecyltrichlorosilane (OTS) and incomplete self-assembled monolayers (SAM) immersed in hexadecane by using total internal reflection and fluorescence recovery after photo-bleaching (TIR-FRAP). They reported that the

Strategy to discover full-length amyloid-beta peptide ligands using high-efficiency microarray technology

• Clelia Galati,
• Natalia Spinella,
• Lucio Renna,
• Danilo Milardi,
• Francesco Attanasio,
• Michele Francesco Maria Sciacca and
• Corrado Bongiorno

Beilstein J. Nanotechnol. 2017, 8, 2446–2453, doi:10.3762/bjnano.8.243

• concentrations of peptide are used achieving a relatively inexpensive assay. Here, the HES is a silicon-based substrate able to act as a fluorescence amplifier. The intensity of the fluorescence is increased by exploiting the constructive interference phenomena of the electric field of light in the near-surface

• residues of the peptide. The propensity of the epoxysilane HESs to immobilize peptides was investigated by measuring the fluorescence of 1 μM Cy3-Aβ40 spot arrays previously assayed until the blocking step. For comparison, reference HESs properly prepared with an O2-plasma treatment have been tested. In

• standard microarray protocol (described in the Experimental section). After BSA blocking, the slides were scanned in order to check the fluorescence of the amyloid spots after the extensive washing involved in the blocking process. As shown in Figure 2A, bright amyloid spots are observed on the

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• intracellularly within minutes (Figure 3). The uptake rate, encoded in the slope of the intracellular mean fluorescence intensity over time (Δ), was comparable between the single exposure experiments but was lower in the co-exposure experiment: roughly half for both AuNPs and FeOxNPs (Figure 3). However, the

• cases. These positive correlations between uptake and time are confirmed by the LSM data. A continuous increase in the number of voxels associated with a fluorescence signal (i.e., the uptake load) within the investigated time window was found, independent of particle type or exposure mode (Figure 5

• to single-exposure experiments after the same time. The intracellular mean fluorescence intensity did not change over the course of the experiment: no significant difference (p < 0.05) in intracellular mean fluorescent intensity between 2 h, 6 h and 24 h for any of the NP types nor for any exposure

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2017, 8, 2296–2306, doi:10.3762/bjnano.8.229

• YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization of a few fluorescent events per DNA per frame of the video. Thus, controlling the fluorescence on

• theoretically predicted with the proposed method in this report. Keywords: diffusion; PAINT; single-molecule photophysics; super-resolution imaging; Introduction Fluorescence imaging of DNA with intercalating dyes is important for DNA sensing [1][2], nucleic acid imaging inside cells and viruses [3][4][5

• YOYO-1, low laser power is also desired to reduce photodamage to the immobilized DNA and to the YOYO-1 molecules in the bulk solution [39][41]. Thus, the effect of laser power is an important parameter to control during imaging to maintain single-molecule fluorescence while also preserving the DNA from

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• boron dipyrromethene (BODIPY) dye with on/off modulated fluorescence emission activated by an acidic environment. The switching properties are linked to the photoinduced electron transfer (PET) characteristics of the dimethylamino functionalities attached to the BODIPY core. The on/off emission of the

• fluorescent CNOs is fast and reversible both in solution and in vitro, making this nanomaterial suitable as pH-dependent probes for diagnostic applications. Keywords: carbon nanomaterials; fluorescence; imaging; nanomedicine; nano-onion; Introduction Nanomaterial-based probes (nano-probes) that are able to

• , Scheme 1), which are capable of introducing photoinduced electron transfer (PET) [27] properties to the fluorophore, the dye molecule exhibited a bright red fluorescence with maximum emission centered at 637 nm in chloroform (Figure 1). These PET groups were activated in neutral or basic environment

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• biochemical properties of tissues. The different methods, including spectroscopic (diffuse reflectance spectroscopy, induced fluorescence and autofluorescence spectroscopy, Raman spectroscopy) and imaging techniques (confocal microscopy, optical coherence tomography, Mueller matrix imaging polarimetry

• such as diffuse reflectance spectroscopy, fluorescence spectroscopy, Raman spectroscopy, in vivo confocal microscopy, optical coherence tomography and multi-wavelength imaging Mueller polarimetry, as well as the combination of different techniques have been explored to improve the detection of cervical

• parameters are linked to the size and density of the scatterers, total hemoglobin (Hb) concentration and Hb saturation with oxygen, which can be used as optical markers to assess and grade CIN lesion. The principle of using diffuse reflectance and fluorescence spectroscopy for tissue diagnostics is

Self-assembly of chiral fluorescent nanoparticles based on water-soluble L-tryptophan derivatives of p-tert-butylthiacalix[4]arene

• Pavel L. Padnya,
• Irina A. Khripunova,
• Olga A. Mostovaya,
• Timur A. Mukhametzyanov,
• Vladimir G. Evtugyn,
• Vyacheslav V. Vorobev,
• Yuri N. Osin and
• Ivan I. Stoikov

Beilstein J. Nanotechnol. 2017, 8, 1825–1835, doi:10.3762/bjnano.8.184

• feature of the compounds is the pronounced dependence of their spectral (emission and chiroptical) properties on the polarity of the solvent and the length of the linker between the macrocyclic and fluorophore parts of the molecule. Keywords: fluorescence; nanoparticles; self-assembly; thiacalixarene

• acid with significant fluorescence [43]. The ability to fluoresce is due to the presence of the indole groups in the structure of the compounds 8–11. Currently, fluorescently active, water-insoluble tryptophan derivatives of the calix[4]arene, capable of selective binding of fluoride anions, some metal

• region in comparison with the compounds 10 and 11 (349 nm) and the initial ester 2 (Figure 3A, Supporting Information File 1, Figure S29). The dependence of fluorescence on the conformation of macrocycles was virtually absent for both pairs of the compounds 8, 9 and 10, 11. The intensity of the emission

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• (UC) emission intensity of Tween 80-coated nanoparticles in comparison with oleic acid coated UCNPs. In addition, the nonspecific uptake and distribution of non-targeted Tween 80-coated UCNPs in human MCF-7 and MDB-MA-231 breast cancer cells was visualized by using confocal fluorescence microscopy

Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications

• Arūnas Jagminas,
• Agnė Mikalauskaitė,
• Vitalijus Karabanovas and
• Jūrate Vaičiūnienė

Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174

• a reddish-pink solution was obtained after 20 min processing (see inset in Figure 3). This process is most likely due to the stronger capping of Au NPs with methionine molecules than with CoFe2O4@Met/Au NPs. Note that no fluorescence was seen under UV and blue-light excitation of this solution

Collembola cuticles and the three-phase line tension

• Håkon Gundersen,
• Hans Petter Leinaas and
• Christian Thaulow

Beilstein J. Nanotechnol. 2017, 8, 1714–1722, doi:10.3762/bjnano.8.172

• the dye. The parts of the cuticle that were wetted by the dye can then be visualized with fluorescence microscopy. Results and Discussion Collembola cuticles were dyed with a water–acetone solution of Nile Red and imaged with fluorescence microscopy, a selection of cuticles are shown in Figure 4. The

• model of Zheng et al. [16] for . A system with θ0 = 105°, S = 0.1 μm and f = 0.25 is shown as a red line in each graph. Stained samples imaged with fluorescence microscopy, showing the tops of primary and (present in d and g) secondary granules. The light areas are those where the lipophilic dye has

• bonded with the surface, indicating wetting contact between the dye solution and a lipid layer. The images were obtained with confocal fluorescence microscopy using incidental light of 488 nm wavelength and a bandpass filter (565–615 nm). a) C. clavatus (winter-acclimated), b) C. clavatus (summer

Three-in-one approach towards efficient organic dye-sensitized solar cells: aggregation suppression, panchromatic absorption and resonance energy transfer

• Jayita Patwari,
• Samim Sardar,
• Bo Liu,
• Peter Lemmens and
• Samir Kumar Pal

Beilstein J. Nanotechnol. 2017, 8, 1705–1713, doi:10.3762/bjnano.8.171

• , using time-resolved fluorescence decay measurements. The electron transfer time scales from the dyes to TiO2 have also been characterized for each dye. The current–voltage (I–V) characteristics and the wavelength-dependent photocurrent measurements of the co-sensitized DSSCs reveal that FRET between the

• the time-resolved fluorescence decay of the mixture of SQ2 and PPIX, FRET from PPIX to SQ2 has been confirmed. The DSSCs were co-sensitized with different molar ratios of the two dyes. The FRET-enhanced photocurrent and the anti-aggregating properties of PPIX have been experimentally proven to be the

• proximity. To create this proximity between these two dyes Al2O3 has been used. The excited-state lifetime of PPIX (attached to Al2O3) was measured in presence and absence of SQ2 by fitting the time-resolved fluorescence decays (Figure 2b). It can be noted from the lifetime components summarized in Table 1

Group-13 and group-15 doping of germanane

• Nicholas D. Cultrara,
• Maxx Q. Arguilla,
• Shishi Jiang,
• Chuanchuan Sun,
• Michael R. Scudder,
• R. Dominic Ross and
• Joshua E. Goldberger

Beilstein J. Nanotechnol. 2017, 8, 1642–1648, doi:10.3762/bjnano.8.164

• are retained on the germanane lattice after topotactic deintercalation. Using X-ray fluorescence (XRF) and X-ray photoelectron spectroscopy (XPS) we show that up to 1.1% and 2.3% of As and Ga, respectively, can be substituted onto the germanane lattice. In contrast to pristine GeH, these materials

• measured using X-ray fluorescence on an Olympus X-5000 Mobile XRF System. SEM and EDX were performed using a FEI Helios Nanolab 600 dual beam focussed ion beam/scanning electron microscope. X-ray photoelectron spectroscopy was performed using a Kratos Axis ultra X-ray photoelectron spetrometer with a

Development of an advanced diagnostic concept for intestinal inflammation: molecular visualisation of nitric oxide in macrophages by functional poly(lactic-co-glycolic acid) microspheres

• Kathleen Lange,
• Christian Lautenschläger,
• Maria Wallert,
• Stefan Lorkowski,
• Andreas Stallmach and
• Alexander Schiller

Beilstein J. Nanotechnol. 2017, 8, 1637–1641, doi:10.3762/bjnano.8.163

• Salmonella typhimurium. After treatment with NO550 microparticles, only activated cells caused a green particle fluorescence and could be detected by laser scanning microscopy. NO release was confirmed indirectly with Griess reaction. Our functional NO550 particles enable a simple and early evaluation of

• abiotic and biotic experiments. To our knowledge, this is the first time of visualisation of NO at different concentrations via fluorescence-emitting NO550-PLGA microspheres. Results and Discussion Physicochemical characterisation of microspheres The preparation of NO550-loaded microspheres was a reliable

• an up to 8-fold increased fluorescence signal at 550 nm compared to inactive NO550-loaded microspheres (see Section 3 of Supporting Information File 1 for further experimental data). Thus, NO550-loaded particles could sense abiotic NO by reacting with the encapsulated NO550. The conversion into the

Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots

• Maria C. Cringoli,
• Slavko Kralj,
• Marina Kurbasic,
• Massimo Urban and
• Silvia Marchesan

Beilstein J. Nanotechnol. 2017, 8, 1553–1562, doi:10.3762/bjnano.8.157

• excellent water solubility, ease of functionalization, high chemical stability and resistance to photo-bleaching. In particular, CNDs have attracted particular interest in light of their biocompatibility, combined with their fascinating fluorescence properties, such as excitation-dependent emission range

• interesting method to control fluorescence quenching upon application of a specific trigger, and to introduce new physical and optical properties of interest [7][8]. Such systems can be useful in several applications, such as bacteria detection [9], sensing of reactive oxygen species (ROS) and screening for

• address demanding therapeutic challenges [18]. In 2015, Steed et al. reported CND–hydrogel hybrids obtained from bis(urea) derivatives used as LMWGs [19] that displayed considerable fluorescence enhancement relative to CNDs alone and showed promising performance in silver ion selective determination [20

A biofunctionalizable ink platform composed of catechol-modified chitosan and reduced graphene oxide/platinum nanocomposite

• Peter Sobolewski,
• Agata Goszczyńska,
• Małgorzata Aleksandrzak,
• Karolina Urbaś,
• Joanna Derkowska,
• Agnieszka Bartoszewska,
• Jacek Podolski,
• Ewa Mijowska and
• Mirosława El Fray

Beilstein J. Nanotechnol. 2017, 8, 1508–1514, doi:10.3762/bjnano.8.151

• of concept, we reacted our amine-terminated DNA oligonucleotide probes for Group B streptococcus (GBS) to the printed, annealed nanocomposite ink stripes using glutaraldehyde as a linker [15]. Next, the modified structures were allowed to hybridize with Cy3-fluorescence-labeled, PCR-amplified

• use in non-viral gene delivery [16]. As this is a proof-of-concept study, we utilized confocal microscopy in order to assess the hybridization of Cy3 fluorescence-labeled complementary PCR product (Figure 4). This method, while not practical for real-world biosensing applications, allowed us to