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Search for "overproduction" in Full Text gives 8 result(s) in Beilstein Journal of Organic Chemistry.
Discovery and biosynthesis of bacterial drimane-type sesquiterpenoids from Streptomyces clavuligerus
Beilstein J. Org. Chem. 2024, 20, 815–822, doi:10.3762/bjoc.20.73
- File 1). To validate the function of the presumed DMS, we cloned its gene into the pETDuet-1 vector to form plasmid pLD10050, which was transformed into our truncated artificial FPP-overproduction system in E. coli to yield strain DL10092 [34]. Analysis of the fermentation extracts revealed that
Synthesis of C6-modified mannose 1-phosphates and evaluation of derived sugar nucleotides against GDP-mannose dehydrogenase
Beilstein J. Org. Chem. 2022, 18, 1379–1384, doi:10.3762/bjoc.18.142
- pathogen in these cases is mucoid Pseudomonas aeruginosa. Such infections are characterised by overproduction of the exopolysaccharide alginate. We present herein the design and chemoenzymatic synthesis of sugar nucleotide tools to probe a critical enzyme within alginate biosynthesis, GDP-mannose
- of alginate overproduction contributes to the PA biofilm environment and the resultant and deleterious bacterial resistance to current antibiotic treatments [2]. Alginate production is therefore established as a major virulence factor within PA respiratory tract infections for CF sufferers, and
Efficient production of clerodane and ent-kaurane diterpenes through truncated artificial pathways in Escherichia coli
Beilstein J. Org. Chem. 2022, 18, 881–888, doi:10.3762/bjoc.18.89
- -kaurane skeletons outlined here may provide an attractive route to prepare other privileged diterpene scaffolds. Keywords: artificial pathway; ent-kaurene; Escherichia coli; overproduction; terpentetriene; Introduction Diterpenoids, of which there are over 34,000 members (http://terokit.qmclab.com
- produce IPP and DMAPP [19][20][21]. Notably, this pathway successfully bypassed the limitations of native isoprenoid biosynthetic pathways, resulting in the overproduction of multiple (mero)terpenoids such as lycopene, cis-abienol, and prenylated tryptophan [15][19][22][23]. The clerodane and ent-kaurane
β-Lactamase inhibition profile of new amidine-substituted diazabicyclooctanes
Beilstein J. Org. Chem. 2021, 17, 711–718, doi:10.3762/bjoc.17.60
- case of the ceftazidime/avibactam combination due to overproduction of AmpC cephalosporinases [26]. In another report it has been concluded that ESBLs of the GES, PER and BEL types in E. coli and P. aeruginosa conferred resistance against sulbactam and avibactam combinations [27]. Therefore, it is
Fabclavine diversity in Xenorhabdus bacteria
Beilstein J. Org. Chem. 2020, 16, 956–965, doi:10.3762/bjoc.16.84
- via conjugation, with Escherichia coli as a donor strain, followed by homologous recombination as described previously [14][22]. This led to a formal ‘knock out’ of the BGC and no production of the respective natural product without induction, whereas induced mutants showed mostly an overproduction of
Genome mining in Trichoderma viride J1-030: discovery and identification of novel sesquiterpene synthase and its products
Beilstein J. Org. Chem. 2019, 15, 2052–2058, doi:10.3762/bjoc.15.202
- terpenoids have rarely been studied and the low concentrations of products under natural conditions have limited the pace of research in this field. Metabolic engineering makes the overproduction of different terpenoids from T. viride possible [21][24]. To increase the discovery efficiency of terpenoid
- metabolic engineering strategy was used to reconstruct an FPP overproduction platform in S. cerevisiae in order to obtain sufficient quantities of the products of Tvi09626 for chemical structural characterisation. S. cerevisiae YZL141, engineered previously [21], was used owing to its ability to provide
- synthase, Tvi09626, from T. viride using a strong sesquiterpene overproduction platform with S. cerevisiae YZL141; it is the first biochemically identified and characterised sesquiterpene synthase from this filamentous fungus. In an analysis of its relative structure, the product of this enzyme was
Intermediates in monensin biosynthesis: A late step in biosynthesis of the polyether ionophore monensin is crucial for the integrity of cation binding
Beilstein J. Org. Chem. 2014, 10, 361–368, doi:10.3762/bjoc.10.34
- of product in unoptimised 50 mL shake flask cultures, over 100-fold more than the wild type strain. This overproduction greatly facilitated the characterisation of the products of the ΔmonD and ΔmonE mutants. For polyether production the mutant strains were initially cultivated in the SM-16 medium
Activation of cryptic metabolite production through gene disruption: Dimethyl furan-2,4-dicarboxylate produced by Streptomyces sahachiroi
Beilstein J. Org. Chem. 2013, 9, 1768–1773, doi:10.3762/bjoc.9.205
- inactivation led to the abolishment of azinomycin production, confirming the involvement of aziA2 in the biosynthesis of the antitumor agent [11][13] and also led to overproduction of a compound, dimethyl furan-2,4-dicarboxylate. The metabolite represents one of many cryptic pathway metabolites from the S
- overproduction of the cryptic metabolite dimethyl furan-2,4-dicarboxylate and led to complete abolishment of azinomycin production. It is the first non-azinomycin related metabolite to be reported from the S. sahachiroi strain, a microorganism that has been mined for its natural product constituents since 1954
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