Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

• Gennady L. Burygin,
• Polina I. Abronina,
• Nikita M. Podvalnyy,
• Sergey A. Staroverov,
• Leonid O. Kononov and
• Lev A. Dykman

Beilstein J. Nanotechnol. 2020, 11, 480–493, doi:10.3762/bjnano.11.39

• glyco-GNPs 3 and 4. The TEM data of the prepared Ara6-GNPs 3 and 4 (Figure 2) clearly suggest that coupling of the Ara6 glycosides 1 and 2 with GNPs did not change the size of nanoparticles (d = 15 nm) and that aggregation is absent. The addition of NaCl solution to conjugates of GNPs with Ara6

• or 4, which were purified from the excess of Ara6 glycosides 1 and 2 by centrifugation, or (3) were prepared in 0.9% NaCl from glyco-GNPs 3 or 4, which were purified by centrifugation, dissolved in 5% sucrose solution and then lyophilized. The absorption spectra of these solutions were virtually

• nanoparticles (d = 15 nm) to give the corresponding glyco-GNPs with pendant Ara6 moieties (Ara6C2NH2-GNPs (3) and Ara6C2EG7NH2-GNPs (4), respectively), which were found to be stable in the presence of 0.9% NaCl only in alkaline media (pH 8–10, Figure 3). Noteworthy is the better stabilization of GNPs by Ara6

Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes

• Alfredo Nuñez-Rivera,
• Pierrick G. J. Fournier,
• Danna L. Arellano,
• Ana G. Rodriguez-Hernandez,
• Rafael Vazquez-Duhalt and
• Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28

• ′-GACAAGGACGGGCACAUUAUU-3′. BMV VLPs with siRNA were synthetized as described previously [22] using a mass ratio of 1:6 (siRNA/capsid protein). The disassembled BMV capsid protein and siRNA were mixed and dialyzed overnight in an assembly buffer (50 mM NaCl, 10 mM KCl, 5 mM MgCl2, 1 mM DTT, 50 mM Tris-HCl, pH 7.2

Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications

• Eike Folker Busmann,
• Dailén García Martínez,
• Henrike Lucas and
• Karsten Mäder

Beilstein J. Nanotechnol. 2020, 11, 213–224, doi:10.3762/bjnano.11.16

• the point of forming stable but nonisotonic nanoemulsions, the conductivity of a medium chain triglyceride (MCT)/Kolliphor® HS 15/NaCl solution (20:20:60) was measured as shown in Figure 1. Increasing the salinity of the aqueous phase resulted in a significant decrease of the temperature at the

• incipient phase inversion from 81.5 °C at 0.4 wt % NaCl concentration to 65.4 °C at 5 wt % NaCl concentration. The particles in the nanoemulsion had an average diameter (zave) of 56–59 nm and a remarkably narrow polydispersity index (PDI) distribution of 0.03–0.06. The salinity did not have a significant

• influence on the particle diameter and the PDI of the nanoemulsion formed by shock dilution. Figure 2 shows the particle diameters and the PDIs of different nanoemulsions prepared using 1.75 wt % and 5 wt % NaCl solutions. Nanoemulsions were successfully formed using solutions of the compositions indicated

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• % loading with respect to chitosan, a high siRNA loading for precise gel analysis) or dissolved in water at the same concentration (40 µg/mL) were incubated with 22 µL of a solution of RNAse I (15 mM Tris buffer, 0.3 M NaCl, pH 7.0) at a concentration of 0, 0.33 and 3.33 U (corresponding to 0, 0.5, and 5 U

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

• Dávid Juriga,
• Evelin Sipos,
• Orsolya Hegedűs,
• Gábor Varga,
• Miklós Zrínyi,
• Krisztina S. Nagy and
• Angéla Jedlovszky-Hajdú

Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249

• of the release of metoprolol is diffusion. Moreover, metoprolol can be measured easily beside DTT by UV–vis spectroscopy. The kinetics of the metoprolol release from the different PASP-XCYS-LYS gels was studied in both physiological saline (NaCl) and DTT solution (Figure 7). The graph depicts the

• concentration after ca. 50 min in every measurement. The saturation concentration is highest for the PASP-100CYS-LYS sample reaching a concentration of 11 g/L∙ggel in physiological NaCl. Upon decreasing the density of CYS cross-links in the hydrogels, the saturation concentration is reduced to around 7.5 g/L

Long-term stability and scale-up of noncovalently bound gold nanoparticle-siRNA suspensions

• Anna V. Epanchintseva,
• Julia E. Poletaeva,
• Dmitrii V. Pyshnyi,
• Elena I. Ryabchikova and
• Inna A. Pyshnaya

Beilstein J. Nanotechnol. 2019, 10, 2568–2578, doi:10.3762/bjnano.10.248

• from the gel with water and precipitated as the sodium salt in acetone. The siRNA samples containing complementary oligoribonucleotides (25 µM each) and 350 mM NaCl were subjected to heating for 3 min at 95 °С, followed by cooling to 25 °С. The samples of native siRNA (1) and fluorescently labeled

Microfluidics as tool to prepare size-tunable PLGA nanoparticles with high curcumin encapsulation for efficient mucus penetration

• Nashrawan Lababidi,
• Valentin Sigal,
• Aljoscha Koenneke,
• Konrad Schwarzkopf,
• Andreas Manz and
• Marc Schneider

Beilstein J. Nanotechnol. 2019, 10, 2280–2293, doi:10.3762/bjnano.10.220

• simple model Mucin (1%) was dissolved in water containing 1% NaCl and left overnight under stirring at room temperature to form a kind of artificial mucus (AM). The sample was stored in the refrigerator (4 °C) until use. The AM was incubated with the suspension of PLGA NPs that were stabilized with

Mannosylated brush copolymers based on poly(ethylene glycol) and poly(ε-caprolactone) as multivalent lectin-binding nanomaterials

• Stefania Ordanini,
• Wanda Celentano,
• Anna Bernardi and
• Francesco Cellesi

Beilstein J. Nanotechnol. 2019, 10, 2192–2206, doi:10.3762/bjnano.10.212

• polymers and their glyco-derivatives, once dispersed in aqueous solution. Firstly, the non-mannosylated polymers were tested in three different media (H2O, 10 mM PBS, 0.9% w/v NaCl) and at three different temperatures (10, 25 and 37 °C; concentration of 1 mg/mL, see Supporting Information File 1, Figures

• . Turbidimetric assay This assay was performed in filtered (PTFE, 0.2 μm) HEPES-buffered saline (HBS), containing 10 mM HEPES, 150 mM NaCl and 1 mM CaCl2 at pH 7.4, according to a procedure already reported in the literature [38]. The stock solution of Con A (6 μM with respect to the tetramer, MW = 104000 Da) was

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• chloride hexahydrate (FeCl3·6H2O) with ≥98% purity was purchased from Sigma-Aldrich. Hydrochloric acid (HCl, 36.5–38.0% w/w) and NaCl with 99% purity were acquired from Labsynth (São Paulo, Brazil). Sodium citrate (Na3C6H5O7) with 99.8% purity was purchased from J.T Baker Chemical Company. All aqueous

Microbubbles decorated with dendronized magnetic nanoparticles for biomedical imaging: effective stabilization via fluorous interactions

• Da Shi,
• Justine Wallyn,
• Dinh-Vu Nguyen,
• Francis Perton,
• Delphine Felder-Flesch,
• Sylvie Bégin-Colin,
• Mounir Maaloum and
• Marie Pierre Krafft

Beilstein J. Nanotechnol. 2019, 10, 2103–2115, doi:10.3762/bjnano.10.205

• )piperazine-N′-2-ethanesulfonic acid) were purchased from Sigma-Aldrich (Lyon, France). A HEPES buffer solution (20 mmol L−1) in a 150 mmol L−1 NaCl solution was prepared, and its pH was adjusted to 7.4 with 1 N NaOH. Water was purified using a Millipore system (surface tension 71.4 mN m−1 at 20 °C

Synthesis of highly active ETS-10-based titanosilicate for heterogeneously catalyzed transesterification of triglycerides

• Muhammad A. Zaheer,
• David Poppitz,
• Khavar Feyzullayeva,
• Marianne Wenzel,
• Jörg Matysik,
• Radomir Ljupkovic,
• Aleksandra Zarubica,
• Alexander A. Karavaev,
• Andreas Pöppl,
• Roger Gläser and
• Muslim Dvoyashkin

Beilstein J. Nanotechnol. 2019, 10, 2039–2061, doi:10.3762/bjnano.10.200

• solution was prepared by dilution of the 9.8 mL aqueous solution of 51–55 wt % Na2SiO3 with 45.2 mL of DDW in a 250 mL flask. An aqueous solution of NaCl (3.4 g of NaCl dissolved in 10.0 mL of DDW) was subsequently added into the flask with the silica-containing solution in it. A titania-containing

• solution was further added into 87.5 mL of the titania/silica-containing solution. The resulting molar composition of the obtained 90 mL solution is the following SiO2/TiO2/HCl/Na2O/NaCl/KF/H2O = 5.56:1:1.94:2.64:3.22:0.09:216. The solution was then further aged for 16 h in a flask at room temperature for

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• (1 mg/mL; supplemented with 250 mM NaCl) over 5–10 min under continuous stirring at 500–800 rpm at ambient temperature. PAHCPMV particles were washed four times with DD water (15 mL each) on 50 kDa cut-off Millipore filter membranes, followed by dialysis with 12,400 molecular weight cut-off (MWCO

• NaCl; pH 7.4) was added to a solution of carboxyl-PEG 5000-SH (spacer arm length 15.8 Å, 10 mg) dissolved in DMSO (1 mL). The reaction was stirred for ca. 12 h at ambient temperature. Carboxyl-PEG5000Au-CPMV particles were dialyzed for 24 h against 100 mM sodium phosphate, 0.15 M NaCl, buffer pH 7.4

Nanoporous smartPearls for dermal application – Identification of optimal silica types and a scalable production process as prerequisites for marketed products

• David Hespeler,
• Sanaa El Nomeiri,
• Jonas Kaltenbach and
• Rainer H. Müller

Beilstein J. Nanotechnol. 2019, 10, 1666–1678, doi:10.3762/bjnano.10.162

• rutin active agent powder and the rutin smartPearls was monitored over one hour in 0.15 molar NaCl solution using in situ UV–vis measurements (Sirius inForm®, UK) with a fiber optic collector and a path length of 5 mm. The 0.15 molar NaCl solution was placed in a beaker and the aqueous phase was

• baseline correction and the 0.15 molar NaCl solution used in this study also led to differences from the values reported in the literature. The assumption that nanometer-sized rutin particles are present in the raw material is supported by the fact that the measured Cs value increased from 30 to 70 µg/mL

Stationary beam full-field transmission helium ion microscopy using sub-50 keV He⁺: Projected images and intensity patterns

• Michael Mousley,
• Santhana Eswara,
• Olivier De Castro,
• Olivier Bouton,
• Nico Klingner,
• Christoph T. Koch,
• Gregor Hlawacek and
• Tom Wirtz

Beilstein J. Nanotechnol. 2019, 10, 1648–1657, doi:10.3762/bjnano.10.160

• configuration. Here, we report projection imaging and intensity patterns obtained from powder and bulk crystalline samples using stationary broad-beam as well as convergent-beam illumination conditions in THIM. The He+ ions formed unexpected spot patterns in the far field for MgO, BN and NaCl powder samples

• ] and can open new imaging modalities using transmitted He+ ions. To understand the different processes contributing to the deflected intensity of transmitted He+ ions, we investigated BN, MgO, NaCl and Si samples using an in-house built transmission helium ion microscope (THIM). In this instrument the

• #549649) and NaCl (Plano Product #46-2). A small amount (≈1 mg) of the powder was deposited either from a spatula (BN and MgO) or scratched with a scalpel blade (NaCl) onto TEM grids without any support membrane (finder grids from Ted Pella Product #79750, Maxtaform™ Style H2, 200 mesh Cu grids) moistened

Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidation

• Dominik Wrana,
• Karol Cieślik,
• Wojciech Belza,
• Christian Rodenbücher,
• Krzysztof Szot and
• Franciszek Krok

Beilstein J. Nanotechnol. 2019, 10, 1596–1607, doi:10.3762/bjnano.10.155

• show the properties of a bulk-like crystalline rock-salt TiO phase, unlike previous studies on the crystallography and electronic structure of TiO, which were based on defective thin films formed on various surfaces, e.g., TiC(100) [23] or TiO2(110) [24]. γ-TiO is the high-temperature phase with a NaCl

Janus-micromotor-based on–off luminescence sensor for active TNT detection

• Ye Yuan,
• Changyong Gao,
• Daolin Wang,
• Chang Zhou,
• Baohua Zhu and
• Qiang He

Beilstein J. Nanotechnol. 2019, 10, 1324–1331, doi:10.3762/bjnano.10.131

• oleic acid (OA, 90%) were obtained from Aladdin Chemistry Co. Ltd. NaOH, NH4F, ethanol, methanol, cyclohexane, trichloromethane, acetonitrile, NaCl, Na2HPO4, and NaH2PO4 were purchased from Tianjin Tianli Chemical Reagent Co. Ltd. Hydrofluoric acid (HF) and hydrogen peroxide (H2O2) were obtained from

Synthesis and characterization of quaternary La(Sr)S–TaS₂ misfit-layered nanotubes

• Marco Serra,
• Erumpukuthickal Ashokkumar Anumol,
• Dalit Stolovas,
• Iddo Pinkas,
• Ernesto Joselevich,
• Reshef Tenne,
• Andrey Enyashin and
• Francis Leonard Deepak

Beilstein J. Nanotechnol. 2019, 10, 1112–1124, doi:10.3762/bjnano.10.111

• distorted NaCl structure and the TaS2 can be indexed in a pseudo-hexagonal unit cell [35][38]. Figure 4 shows low-magnification and high-resolution HAADF-STEM images of two LaS–TaS2 (10 atom % Sr in the precursor) nanotubes. One can see the stacking of the SrxLa1−xS (indicated by green lines) and TaS2

Enhanced inhibition of influenza virus infection by peptide–noble-metal nanoparticle conjugates

• Zaid K. Alghrair,
• David G. Fernig and
• Bahram Ebrahimi

Beilstein J. Nanotechnol. 2019, 10, 1038–1047, doi:10.3762/bjnano.10.104

• NaCl, 3 mM KCl, l.8 mM Na2HPO4, 15 mM KH2PO4) with Tween-20 (0.1% (v/v)) pH 7.4 was added to the gold nanoparticles [26] and 10× (100 mM NaNO3, 20 mM HEPES with Tween-20 (0.1% (v/v)) pH 7.4 to the silver nanoparticles [17], vortex-mixed and the (gold or silver) nanoparticles were placed on a rotating

• nanoparticles were then applied to the column, the unbound fraction was recovered. Columns were washed with PBS and eluted with 1 M NaCl and then 2 M NaCl in 8 mM Na2HPO4, 15 mM KH2PO4, pH 7.4. Calculation of the aggregation parameter (AP) The surface plasmon absorption peak of 8.8 nm diameter gold

• with 2 M NaCl) fractions as a percentage of total nanoparticles applied to the column. Results are the mean ± SD (n = 3). Determination of the half maximal inhibitory concentration (IC50) of FluPep and and FluPep ligand in a plaque assay. Inhibition of plaque formation as a function of the

Correlation of surface-enhanced Raman scattering (SERS) with the surface density of gold nanoparticles: evaluation of the critical number of SERS tags for a detectable signal

• Vincenzo Amendola

Beilstein J. Nanotechnol. 2019, 10, 1016–1023, doi:10.3762/bjnano.10.102

• plate placed at the bottom of a cell containing a 10−4 M NaCl solution in double distilled water [18][19][41]. Dye solutions of either hexacyanin 3 (HITC, perchlorate, Exciton), malachite green (MG, oxalate salt, Sigma-Aldrich) or malachite green isothiocyanate (MGITC, Invitrogen) were added to a 2 nM

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• NPs incubated with 500 µL serum was measured in pure water and 0.2 mM NaCl, respectively. After water dilution PLGA NPs and NPs incubated with 500 µL FBS or human serum show zeta potentials of −43.2 mV, −34.0 mV and −21.4 mV, respectively. As could be expected the higher ionic background of 0.2 mM

• NaCl leads to a moderate reduction of the zeta potential to −33.0 mV, −30.5 mV, and −19.4 mV, respectively, but the graduation of the zeta potential values between the different samples remains the same. In brief, the incubation of PLGA NPs with increasing concentrations of FBS enabled us to modulate

Concurrent nanoscale surface etching and SnO₂ loading of carbon fibers for vanadium ion redox enhancement

• Jun Maruyama,
• Shohei Maruyama,
• Tomoko Fukuhara,
• Toru Nagaoka and
• Kei Hanafusa

Beilstein J. Nanotechnol. 2019, 10, 985–992, doi:10.3762/bjnano.10.99

• carbon cloth (ElectroChem). The reference electrode was Ag/AgCl/NaCl (3 M) (0.212 V vs standard hydrogen electrode). The electrolytes were H2SO4 (2 M) and VOSO4 (1 M)/H2SO4 (2 M). The measurements were carried out under Ar atmosphere at 25 °C. A flow cell test was performed using three layers of 3 cm2 of

• -550Air, and TGP-550Air. Cyclic voltammograms in Ar-saturated 2 M H2SO4 at 25 °C for TGP, TGP-550Air, and TGP-CSnPc-TAir (T = 500, 550, 600 and 650 °C). The reference electrode was Ag/AgCl/NaCl (3 M). The counter electrode was carbon cloth. Scan rate: 50 mV·s−1. Cyclic voltammograms in 1 M VOSO4 + 2 M

• H2SO4 at 25 °C for (a,b) VO2+/VO2+, (c) V2+/3+ redox reactions at TGP, TGP-550Air, TGP-CSnPc-TAir (T =500, 550, 600 and 650 °C). The reference electrode was Ag/AgCl/NaCl (3 M). The counter electrode was carbon cloth. Scan rates were (a,b) 1 mV·s−1 and (c) 50 mV·s−1. Charge–discharge curves and cycling

An iridescent film of porous anodic aluminum oxide with alternatingly electrodeposited Cu and SiO₂ nanoparticles

• Menglei Chang,
• Huawen Hu,
• Haiyan Quan,
• Hongyang Wei,
• Zhangyi Xiong,
• Jiacong Lu,
• Pin Luo,
• Yaoheng Liang,
• Jianzhen Ou and
• Dongchu Chen

Beilstein J. Nanotechnol. 2019, 10, 735–745, doi:10.3762/bjnano.10.73

• test sample was immobilized onto a PVC tube with epoxy resin, and then naturally dried for 12 h. After that, a NaCl solution (3.5%) was poured into the PVC tube, and the level of the NaCl solution was in the range of a half to two-thirds of the tube volume. The exposed area of the PVC tube was

Widening of the electroactivity potential range by composite formation – capacitive properties of TiO₂/BiVO₄/PEDOT:PSS electrodes in contact with an aqueous electrolyte

• Konrad Trzciński,
• Mariusz Szkoda,
• Andrzej P. Nowak,
• Marcin Łapiński and
• Anna Lisowska-Oleksiak

Beilstein J. Nanotechnol. 2019, 10, 483–493, doi:10.3762/bjnano.10.49

• anions originating from the electrolyte during electrooxidation/electroreduction cycles [35]. Moreover, the overpotential of EDOT oxidation is lower in NaPSS aqueous electrolyte (in comparison with, e.g., NaCl) [36]. Electrodepositon was performed using 400, short (0.2 s) potentiostatic pulses (E = 1 V

Non-agglomerated silicon–organic nanoparticles and their nanocomplexes with oligonucleotides: synthesis and properties

• Asya S. Levina,
• Marina N. Repkova,
• Nadezhda V. Shikina,
• Zinfer R. Ismagilov,
• Svetlana A. Yashnik,
• Dmitrii V. Semenov,
• Yulia I. Savinovskaya,
• Natalia A. Mazurkova,
• Inna A. Pyshnaya and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2018, 9, 2516–2525, doi:10.3762/bjnano.9.234

• conjugated with oligodeoxyribonucleotides (ODN) through the binding to the negatively charged internucleotide phosphate groups to form Si–NH2·ODN complexes. Like Si–NH2, the Si–NH2·ODN nanocomplexes are dissolved in aqueous solutions. The addition of NaCl did not lead to precipitation. Si–NH2 and Si–NH2·ODN

• these supernatants were precipitated by the addition of 1 µL of 3 M LiClO4 (precipitate 2). The precipitates 1 and 2 were dried at 60 °C to remove the acetone and were dissolved in 1 M NaCl. The amount of the bound (precipitate 1) and unbound (precipitate 2) oligonucleotide was evaluated

• were visualized by StainsAll (Figure 1b). In our experiments, the Si–NH2·ODN nanocomplexes were obtained by mixing 0.46 M Si–NH2 (2 µL) and ODN in 0.16 M NaCl or water (98 µL) while keeping the ratio between the amino and phosphate groups at NH2/p ≥ 10, unless otherwise specified. Formation of

SERS active Ag–SiO₂ nanoparticles obtained by laser ablation of silver in colloidal silica

• Cristina Gellini,
• Francesco Muniz-Miranda,
• Alfonso Pedone and
• Maurizio Muniz-Miranda

Beilstein J. Nanotechnol. 2018, 9, 2396–2404, doi:10.3762/bjnano.9.224

• , Ludox TM-40, pH ≈9 at 25 °C, NaCl content: 0.03 wt %) was diluted with distilled water in order to have a sample with 10 wt % as SiO2 content. Laser ablation of silver in colloidal silica was performed with the fundamental wavelength of a Q-switched Nd:YAG ns-pulsed laser (Quanta System G90-10: rep